##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921393_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2155713
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.187954982875734	31.0	31.0	34.0	26.0	34.0
2	30.261509301098986	31.0	31.0	34.0	26.0	34.0
3	30.306312575004185	31.0	31.0	34.0	26.0	34.0
4	33.763050554503316	37.0	35.0	37.0	28.0	37.0
5	33.76360072050407	37.0	35.0	37.0	28.0	37.0
6	33.80128662767261	37.0	35.0	37.0	29.0	37.0
7	33.712475176426544	37.0	35.0	37.0	28.0	37.0
8	33.68043612484593	37.0	35.0	37.0	28.0	37.0
9	35.21494326934986	39.0	35.0	39.0	28.0	39.0
10-11	35.10749691633348	39.0	35.0	39.0	28.0	39.0
12-13	34.99518627943516	39.0	35.0	39.0	27.0	39.0
14-15	36.10627203157378	40.0	36.0	41.0	27.0	41.0
16-17	36.06016385298043	40.0	36.0	41.0	27.0	41.0
18-19	36.01853887785619	40.0	36.0	41.0	27.0	41.0
20-21	35.96837055767628	40.0	36.0	41.0	27.0	41.0
22-23	35.852537884217426	40.0	36.0	41.0	27.0	41.0
24-25	35.754959959883344	40.0	36.0	41.0	27.0	41.0
26-27	35.613919153430906	40.0	36.0	41.0	26.0	41.0
28-29	35.47395594868148	40.0	36.0	41.0	25.0	41.0
30-31	35.32762640481363	39.5	35.5	41.0	25.0	41.0
32-33	35.15112308549422	39.0	35.0	41.0	24.0	41.0
34-35	34.98473938784987	39.0	35.0	41.0	24.0	41.0
36-37	34.819844988641805	39.0	35.0	41.0	23.5	41.0
38-39	35.03206642071556	39.0	35.0	41.0	24.0	41.0
40-41	35.154928554960705	40.0	35.0	41.0	24.0	41.0
42-43	34.99669482904264	40.0	35.0	41.0	23.0	41.0
44-45	34.881023587091605	40.0	35.0	41.0	22.0	41.0
46-47	34.725907391197254	40.0	35.0	41.0	20.5	41.0
48-49	34.58302635833249	39.0	35.0	41.0	19.0	41.0
50-51	32.90028032488554	37.0	32.5	39.5	15.5	40.0
52-53	33.056863552801325	37.5	32.5	39.5	14.0	40.0
54-55	33.928034251312674	39.0	34.0	40.5	14.0	41.0
56-57	33.96218536512049	39.0	34.0	41.0	13.0	41.0
58-59	33.801217509009774	38.5	34.0	41.0	12.5	41.0
60-61	33.57676091390644	38.0	34.0	40.5	8.5	41.0
62-63	33.30359004190261	37.5	33.5	40.0	7.5	41.0
64-65	33.02792881056059	37.0	33.0	40.0	6.5	41.0
66-67	32.70078206143397	36.5	33.0	40.0	2.0	41.0
68-69	32.34386975446175	36.0	32.5	39.0	2.0	41.0
70-71	31.96824507715081	35.5	32.0	39.0	2.0	41.0
72-73	31.611567727243838	35.0	32.0	38.5	2.0	40.0
74-75	31.20443560900732	35.0	32.0	37.0	2.0	39.5
76-77	30.819664074020984	35.0	31.0	37.0	2.0	39.0
78-79	30.463547559438574	35.0	31.0	36.0	2.0	39.0
80-81	30.100955693081595	35.0	31.0	36.0	2.0	37.5
82-83	29.787565181450404	35.0	31.0	35.5	2.0	37.0
84-85	29.538673747386596	35.0	31.0	35.0	2.0	37.0
86-87	29.299685069394673	35.0	30.0	35.0	2.0	36.0
88-89	29.091451181117336	35.0	30.0	35.0	2.0	36.0
90-91	28.83446057058616	34.0	30.0	35.0	2.0	36.0
92-93	28.643060091951014	34.0	30.0	35.0	2.0	35.0
94-95	28.438129055212823	34.0	30.0	35.0	2.0	35.0
96-97	28.201955455109285	34.0	29.0	35.0	2.0	35.0
98-99	27.974855419065527	34.0	29.0	35.0	2.0	35.0
100-101	26.94002401989504	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81456.0
3	13548.0
4	9042.0
5	6783.0
6	6065.0
7	6175.0
8	6535.0
9	7192.0
10	7720.0
11	8483.0
12	8688.0
13	8713.0
14	9351.0
15	10149.0
16	10570.0
17	11284.0
18	10255.0
19	9383.0
20	9386.0
21	9743.0
22	10753.0
23	12224.0
24	14211.0
25	16556.0
26	19538.0
27	23306.0
28	27111.0
29	32183.0
30	38735.0
31	46589.0
32	57810.0
33	74851.0
34	102138.0
35	146641.0
36	241249.0
37	437983.0
38	502027.0
39	101274.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.12696239397561	19.519116299038096	13.995196268405602	24.358725038580694
2	15.406977418844258	19.897029669881316	40.243185247727105	24.452807663547325
3	18.720322066064572	24.288901502792413	29.97703970455618	27.013736726586835
4	12.79241452391418	16.36792603900887	37.737307217258554	33.10235221981839
5	13.765163395932356	37.50246564000124	35.034876332779945	13.697494631286453
6	32.530547004224495	36.31227424679334	17.523436575673287	13.633742173308866
7	28.106625689620213	32.14489691479771	21.6568237314259	18.091653664156173
8	26.060886700330215	35.2307320407062	20.270094081936705	18.438287177026876
9	26.372713188653325	15.787808219877238	19.837660695635147	38.001817895834286
10-11	25.01380393860008	25.63300918437515	28.585196053047955	20.767990823976813
12-13	25.636232528981672	23.2084680351302	28.267308378525236	22.887991057362896
14-15	22.893336547986706	24.81918154894159	25.526327941518062	26.761153961553642
16-17	22.96277099385236	27.920095719201406	26.297699801884267	22.81943348506197
18-19	22.91866813506104	27.275038819110254	27.645261655767	22.161031390061705
20-21	23.502408818142058	26.491732991818257	27.554861079693428	22.45099711034626
22-23	23.281720713566667	26.313060969321572	27.27704986584772	23.128168451264038
24-25	23.137222142940843	26.48163553228937	27.27717195202742	23.103970372742367
26-27	23.02187133344207	26.7091887813449	27.19073537303678	23.07820451217625
28-29	23.09070316983895	26.795274680865578	27.12882716562048	22.985194983674997
30-31	23.412804180297847	26.540813275405604	27.027348651287475	23.019033893009073
32-33	23.09820851352157	26.61488596268013	26.9628097975389	23.3240957262594
34-35	23.46503512968024	26.647177111161678	26.960977047928488	22.9268107112296
36-37	23.426957353112407	26.533820666249476	26.99142590339285	23.047796077245266
38-39	23.307131289035734	26.707946020448542	26.911095368853623	23.073827321662105
40-41	23.411684177833383	26.475297529787774	26.99431185719034	23.118706435188503
42-43	23.226738996213673	26.503629185232576	26.919944060521754	23.349687758032
44-45	23.236225422356082	26.604035143372734	26.876848298084223	23.28289113618696
46-47	23.39453443299774	26.58396716811004	26.626336445061945	23.39516195383027
48-49	23.192349320125754	26.720788252992655	26.683463555314574	23.403398871567017
50-51	23.30872238182014	26.6939618999323	26.684014951100753	23.313300767146806
52-53	23.488860492714426	26.55931952064489	26.693005693748034	23.258814292892644
54-55	23.125865739492596	26.501506038097183	26.959871521935163	23.41275670047505
56-57	23.229896014126663	26.644854666859125	26.739247816068872	23.38600150294534
58-59	23.244227613553427	26.566226135301857	26.867663944142546	23.32188230700217
60-61	23.343939724642926	26.5827825006398	26.86903651987635	23.204241254840923
62-63	23.297100480793258	26.488535689921473	26.94341236534709	23.27095146393818
64-65	23.094865529764196	26.360833579717983	26.90618044282295	23.638120447694863
66-67	23.116274194496782	26.47580590994038	26.837905557997182	23.570014337565652
68-69	23.253537942223897	26.377380968705094	26.90184076883585	23.467240320235163
70-71	23.136692653568648	26.280286746679987	26.91275604456889	23.67026455518247
72-73	23.10397885048304	26.377798956421184	26.80254153016551	23.71568066293027
74-75	23.12593550719973	26.462284462975582	26.815305170843573	23.59647485898111
76-77	23.09102891767069	26.257964455140403	26.89282024325255	23.75818638393635
78-79	23.101698500475358	26.223015646101956	26.88230904944875	23.79297680397394
80-81	23.13797755170215	26.40884188532599	26.667832095491395	23.785348467480468
82-83	23.159826879436284	26.396841093677864	26.81411391013222	23.62921811675363
84-85	23.221028544437324	26.37187453799742	26.777646467022663	23.629450450542596
86-87	23.02819409725322	26.450228588221293	26.779975002231943	23.741602312293544
88-89	23.020320423952263	26.270290955699043	26.792861312751974	23.91652730759672
90-91	23.037269388307383	26.2538250259574	26.82953763974335	23.879367945991866
92-93	23.069526099285934	26.11411923323459	27.031296172427126	23.78505849505235
94-95	23.002615883645493	26.04092404636508	26.99426830827898	23.96219176171044
96-97	22.958720166822342	25.918454140248983	27.056707830470145	24.066117862458533
98-99	22.8309798409925	25.813643396794504	27.020579834482472	24.334796927730522
100-101	22.95751972130906	25.76902990644731	27.088542783254177	24.184907588989457
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	67.0
1	51.0
2	54.0
3	80.0
4	78.0
5	94.5
6	156.0
7	334.5
8	507.0
9	527.0
10	459.0
11	410.0
12	448.0
13	497.0
14	578.5
15	666.0
16	741.0
17	869.0
18	948.5
19	1044.0
20	1180.0
21	1309.0
22	1476.0
23	1732.5
24	2103.5
25	2652.5
26	3561.5
27	4910.0
28	6713.0
29	9263.0
30	12885.5
31	17854.5
32	23963.0
33	29344.5
34	34316.5
35	40172.5
36	47406.0
37	54222.5
38	59950.0
39	65132.5
40	69006.5
41	72771.0
42	76692.5
43	80934.5
44	84727.5
45	88737.0
46	91267.0
47	91727.5
48	92712.5
49	92988.0
50	90878.0
51	87789.5
52	85206.0
53	82112.0
54	78806.0
55	74325.5
56	67375.5
57	59577.0
58	52883.5
59	45730.0
60	37428.0
61	30414.0
62	24579.5
63	18459.0
64	13067.5
65	9390.5
66	6898.5
67	5207.5
68	3996.0
69	3069.5
70	2229.5
71	1572.5
72	1123.5
73	829.0
74	630.0
75	456.5
76	322.5
77	224.0
78	164.0
79	123.5
80	94.0
81	74.5
82	64.5
83	56.0
84	43.0
85	36.5
86	28.0
87	22.5
88	23.5
89	20.0
90	13.5
91	9.0
92	12.0
93	11.0
94	10.0
95	10.5
96	8.0
97	7.5
98	4.5
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05278995858910717
2	0.06058320379382599
3	0.05214052148871394
4	0.05937710632166712
5	0.05102720074518268
6	0.019854219926307443
7	0.01669981115296888
8	0.02152420104160433
9	0.021988084684742355
10-11	0.0246322214506291
12-13	0.02987410661808877
14-15	0.04622600503870413
16-17	0.030593126264952707
18-19	0.024330697082589378
20-21	0.02832009641357639
22-23	0.04972832654439621
24-25	0.0436282566371312
26-27	0.03142811682260115
28-29	0.01994699665493505
30-31	0.012455275818255956
32-33	0.00985752741668302
34-35	0.009254478680603587
36-37	0.0015772043866692832
38-39	0.00723658483295318
40-41	0.004105370241771516
42-43	0.22345275089958638
44-45	0.19670985887267925
46-47	0.20364491933759274
48-49	0.20051370474641106
50-51	0.1994467723671936
52-53	0.20438713316661355
54-55	0.20285631714425809
56-57	0.21354883511858955
58-59	0.20981457179132845
60-61	0.21577547660565202
62-63	0.2124819027393721
64-65	0.21361841766506023
66-67	0.20278673459778734
68-69	0.2020909091330803
70-71	0.18499679688344414
72-73	0.19364822682796828
74-75	0.1920246340769852
76-77	0.19121283770149367
78-79	0.21793253554624387
80-81	0.22542425638292293
82-83	0.2246124600074314
84-85	0.22039111885487542
86-87	0.2375548136509823
88-89	0.2362791336323527
90-91	0.2353745605282336
92-93	0.23873771694098425
94-95	0.24994050692276754
96-97	0.2505667498410039
98-99	0.24054686314922258
100-101	0.2492910698223743
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2155713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.78875956416248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.02159123208071	53.96098744637376
2	10.538686064416817	13.866541672282157
3	3.1364659915940702	6.190326210064644
4	1.3668629135485542	3.596968623064657
5	0.7557809220440682	2.4860944681769115
6	0.4788061062745026	1.8900035882127648
7	0.3304260266870914	1.5216822896421014
8	0.24158239807043858	1.2714725041271902
9	0.176204399009988	1.0433041956554265
>10	0.8965682813441048	10.34050944359375
>50	0.04299093982380706	1.9116472310275492
>100	0.01304373564163989	1.4346126635645031
>500	8.494195407740348E-4	0.3651905556188303
>1k	1.4156992346231025E-4	0.12065910859579655
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	4.6388364313802443E-5
10-11	0.0	0.0	0.0	6.958254647070366E-5	1.159709107845061E-4
12-13	0.0	0.0	0.0	9.277672862760489E-5	1.6235927509830855E-4
14-15	0.0	0.0	0.0	9.277672862760489E-5	1.8555345725520977E-4
16-17	0.0	0.0	0.0	9.277672862760489E-5	2.319418215690122E-4
18-19	0.0	0.0	0.0	1.159709107845061E-4	2.319418215690122E-4
20-21	0.0	2.3194182156901222E-5	0.0	1.3916509294140732E-4	2.7833018588281465E-4
22-23	0.0	4.6388364313802443E-5	0.0	2.319418215690122E-4	2.7833018588281465E-4
24-25	0.0	4.6388364313802443E-5	0.0	3.247185501966171E-4	2.7833018588281465E-4
26-27	0.0	4.6388364313802443E-5	0.0	5.33466189608728E-4	3.0152436803971585E-4
28-29	0.0	4.6388364313802443E-5	0.0	0.0019715054833366035	3.247185501966171E-4
30-31	0.0	4.6388364313802443E-5	0.0	0.006865477918442761	3.247185501966171E-4
32-33	4.6388364313802443E-5	4.6388364313802443E-5	0.0	0.018045073718069148	3.7110691451041955E-4
34-35	1.159709107845061E-4	6.958254647070366E-5	0.0	0.03312129212005494	4.1749527882422194E-4
36-37	1.3916509294140732E-4	9.277672862760489E-5	0.0	0.05837975648892037	4.1749527882422194E-4
38-39	1.3916509294140732E-4	9.277672862760489E-5	0.0	0.10305175132311212	5.102720074518268E-4
40-41	1.3916509294140732E-4	9.277672862760489E-5	0.0	0.15250174768162553	5.102720074518268E-4
42-43	1.8555345725520977E-4	9.277672862760489E-5	0.0	0.20580197827818453	5.102720074518268E-4
44-45	1.8555345725520977E-4	9.277672862760489E-5	0.0	0.26951639666319216	5.102720074518268E-4
46-47	1.8555345725520977E-4	9.277672862760489E-5	0.0	0.3443640224835124	5.102720074518268E-4
48-49	1.8555345725520977E-4	9.277672862760489E-5	0.0	0.4272600295122774	5.102720074518268E-4
50-51	1.8555345725520977E-4	9.277672862760489E-5	0.0	0.511408522377515	5.102720074518268E-4
52-53	1.8555345725520977E-4	9.277672862760489E-5	0.0	0.6020745804288419	5.566603717656293E-4
54-55	1.8555345725520977E-4	9.277672862760489E-5	0.0	0.7035027390009709	5.566603717656293E-4
56-57	1.8555345725520977E-4	9.277672862760489E-5	0.0	0.8229527771090122	6.262429182363329E-4
58-59	1.8555345725520977E-4	1.159709107845061E-4	0.0	0.9538375470204058	6.494371003932342E-4
60-61	2.319418215690122E-4	1.8555345725520977E-4	0.0	1.0952524756310327	6.494371003932342E-4
62-63	2.319418215690122E-4	1.8555345725520977E-4	0.0	1.2398218130149978	6.958254647070366E-4
64-65	2.319418215690122E-4	1.8555345725520977E-4	0.0	1.4044541179646828	6.958254647070366E-4
66-67	2.319418215690122E-4	2.08747639412111E-4	0.0	1.5914224203314635	6.958254647070366E-4
68-69	2.319418215690122E-4	2.319418215690122E-4	0.0	1.797015650970236	6.958254647070366E-4
70-71	2.319418215690122E-4	2.319418215690122E-4	0.0	2.01599192471354	6.958254647070366E-4
72-73	2.319418215690122E-4	2.319418215690122E-4	0.0	2.2415785403715613	7.190196468639378E-4
74-75	2.319418215690122E-4	2.319418215690122E-4	0.0	2.480664170044899	7.422138290208391E-4
76-77	2.319418215690122E-4	2.319418215690122E-4	0.0	2.7544946845892753	7.422138290208391E-4
78-79	2.319418215690122E-4	2.319418215690122E-4	0.0	3.0559030817182062	7.422138290208391E-4
80-81	2.319418215690122E-4	2.319418215690122E-4	0.0	3.3865361483648333	7.654080111777403E-4
82-83	2.319418215690122E-4	2.319418215690122E-4	0.0	3.730807394119718	7.886021933346415E-4
84-85	2.319418215690122E-4	2.319418215690122E-4	0.0	4.092219140488552	7.886021933346415E-4
86-87	2.319418215690122E-4	2.7833018588281465E-4	0.0	4.485453304776656	8.117963754915426E-4
88-89	2.319418215690122E-4	2.7833018588281465E-4	0.0	4.9088399058687315	8.349905576484439E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1805	0.0	48.898	1
GTATCAA	3215	0.0	42.212437	1
TCAACGC	3950	0.0	33.27962	4
CAACGCA	4090	0.0	32.253498	5
ATCAACG	4115	0.0	31.94372	3
TATCAAC	4275	0.0	31.526655	2
AACGCAG	4325	0.0	30.382113	6
GTACATG	5500	0.0	27.867317	1
TACATGG	5755	0.0	25.810398	2
ACGCAGA	5170	0.0	24.685762	7
ACATGGG	5870	0.0	24.333464	3
CGCAGAG	5255	0.0	23.929218	8
CATGGGG	4820	0.0	22.152931	4
GTATACG	110	0.0028950754	21.569134	1
TATACCG	200	5.44318E-7	21.353441	5
GCAGAGT	5975	0.0	19.537214	9
AGAGTAC	5135	0.0	18.806131	10-11
ACTGCGT	395	1.8189894E-11	18.019783	8
ATGGGGG	3045	0.0	17.60948	5
TAAGGTG	1085	0.0	17.493858	5
>>END_MODULE