FastQCFastQC Report
Mon 15 Aug 2016
SRR2921393_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921393_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155713
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20100.050.3651661
GTATCAA35300.041.0651
TCAACGC45300.030.6930774
CAACGCA45800.030.3085385
ATCAACG47400.029.5334853
TATCAAC48550.028.833932
AACGCAG48450.028.746916
ACGCAGA57200.023.9339187
GTACATG55500.023.5497551
CGCAGAG58800.022.6359168
TACATGG58050.022.3167742
ACATGGG56950.022.0812243
CATGGGG47050.019.6673584
AGAGTAC57150.019.42173410-11
GCAGAGT66300.018.8564349
GTACTAC5450.017.4413531
GTACACT16650.016.8154624
CAGAGTA63550.016.6804079
TACACTG24200.016.5010495
TAAGGTG10450.016.3769825
Produced by FastQC (version 0.11.5)