##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921393_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2155713
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.462686359455084	33.0	31.0	34.0	30.0	34.0
2	31.597930243961045	34.0	31.0	34.0	29.0	34.0
3	31.671167729656034	34.0	31.0	34.0	28.0	34.0
4	35.17003330220674	37.0	35.0	37.0	33.0	37.0
5	34.92714429054332	37.0	35.0	37.0	32.0	37.0
6	34.890159311559565	37.0	35.0	37.0	32.0	37.0
7	34.76086983749692	37.0	35.0	37.0	32.0	37.0
8	34.72430977593029	37.0	35.0	37.0	32.0	37.0
9	36.35264666493174	39.0	37.0	39.0	32.0	39.0
10-11	36.20269534952009	39.0	37.0	39.0	32.0	39.0
12-13	36.14696367280803	39.0	37.0	39.0	32.0	39.0
14-15	37.394931050654705	40.0	38.0	41.0	32.0	41.0
16-17	37.38256112942679	40.0	38.0	41.0	32.0	41.0
18-19	37.35489534089186	40.0	38.0	41.0	32.0	41.0
20-21	37.327646583752106	40.0	38.0	41.0	31.5	41.0
22-23	37.221827766497675	40.0	38.0	41.0	31.0	41.0
24-25	37.16139277352783	40.0	38.0	41.0	31.0	41.0
26-27	37.02807980468643	40.0	37.0	41.0	31.0	41.0
28-29	36.87790211405692	40.0	37.0	41.0	30.0	41.0
30-31	36.74329003907292	40.0	37.0	41.0	30.0	41.0
32-33	36.68686601602347	40.0	37.0	41.0	30.0	41.0
34-35	36.8109323922062	40.0	37.0	41.0	30.0	41.0
36-37	36.791780028231955	40.0	37.0	41.0	30.0	41.0
38-39	36.70518895604378	40.0	37.0	41.0	30.0	41.0
40-41	36.589521193220065	40.0	37.0	41.0	30.0	41.0
42-43	36.4900455209019	40.0	37.0	41.0	30.0	41.0
44-45	36.35064941390621	40.0	37.0	41.0	29.5	41.0
46-47	36.198416486795786	40.0	36.0	41.0	29.0	41.0
48-49	36.04899214320274	40.0	36.0	41.0	28.0	41.0
50-51	35.90343821278621	40.0	36.0	41.0	28.0	41.0
52-53	35.745305149618716	40.0	35.0	41.0	28.0	41.0
54-55	35.55311630073206	39.0	35.0	41.0	27.0	41.0
56-57	35.30169577304586	39.0	35.0	41.0	26.5	41.0
58-59	35.09471321089589	39.0	35.0	41.0	26.0	41.0
60-61	34.836970876921	38.5	35.0	40.0	26.0	41.0
62-63	34.55660169048477	38.0	34.5	40.0	26.0	41.0
64-65	34.2525324567788	37.0	34.0	40.0	26.0	41.0
66-67	33.92032241768733	37.0	34.0	40.0	25.0	41.0
68-69	33.56177468893122	36.0	34.0	39.0	24.0	41.0
70-71	33.181083938353574	36.0	34.0	39.0	23.0	40.5
72-73	32.779087243988414	35.0	33.0	38.5	22.0	40.0
74-75	32.36140873112515	35.0	33.0	37.0	21.5	39.5
76-77	29.569511804215125	33.0	29.0	34.5	15.0	36.5
78-79	31.221214744263264	34.5	31.5	36.0	19.0	38.5
80-81	31.405419459826053	35.0	32.0	36.0	20.0	37.5
82-83	31.225918524404683	35.0	32.0	35.5	19.5	37.0
84-85	30.97928782727571	35.0	32.0	35.0	18.0	37.0
86-87	30.722253611682074	35.0	32.0	35.0	16.0	36.0
88-89	30.427470864628084	35.0	32.0	35.0	8.0	36.0
90-91	30.23368416853264	35.0	32.0	35.0	4.5	35.5
92-93	30.084493390353913	34.0	32.0	35.0	2.0	35.0
94-95	29.98491844693612	34.0	32.0	35.0	2.0	35.0
96-97	29.790325057185257	34.0	31.0	35.0	2.0	35.0
98-99	29.528513072009122	34.0	31.0	35.0	2.0	35.0
100-101	28.44831825943435	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36298.0
3	12201.0
4	6833.0
5	4655.0
6	4124.0
7	4211.0
8	4380.0
9	4794.0
10	5439.0
11	6036.0
12	6417.0
13	7058.0
14	7906.0
15	8882.0
16	9829.0
17	10679.0
18	9741.0
19	8467.0
20	7872.0
21	7790.0
22	8166.0
23	9119.0
24	10621.0
25	12976.0
26	15587.0
27	18753.0
28	22425.0
29	26993.0
30	32737.0
31	40690.0
32	52085.0
33	68358.0
34	97603.0
35	144948.0
36	252224.0
37	500090.0
38	583350.0
39	85370.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.79387554944761	20.85440875232126	14.491246703082016	24.86046899514911
2	14.827014988586113	21.485990443042585	38.4952129394208	25.191781628950505
3	17.3302411934089	25.509551288682868	29.8563305441291	27.30387697377913
4	12.595141326650614	17.699267353216655	36.709301176604846	32.99629014352789
5	13.642716478800827	37.917269118048296	34.90439351783968	13.5356208853112
6	32.37829274685309	37.10783347288681	17.160624117159976	13.353249663100128
7	28.009704459319668	32.42574372248586	21.5832834657723	17.981268352422173
8	26.101389688462433	35.93785299464712	19.88766333142054	18.0730939854699
9	26.214157450458387	15.872474675432214	19.666207885743603	38.247159988365794
10-11	24.95605196537283	26.102334661700553	28.425572543455207	20.51604082947141
12-13	25.599676208015477	23.45153905566856	28.28100319848959	22.66778153782637
14-15	22.75047666764088	25.115675986620833	25.4577864992879	26.67606084645039
16-17	22.87698125531149	28.294538582291445	26.21403495099513	22.614445211401936
18-19	22.899954214472032	27.474401622115845	27.667656444324656	21.957987719087463
20-21	23.4291286249116	26.62961125449131	27.565065578755394	22.3761945418417
22-23	23.22572833942994	26.43186683171681	27.29093474372811	23.051470085125146
24-25	23.031370684356407	26.75642200238904	27.285096315539214	22.92711099771533
26-27	23.036799556143364	26.87387707936025	27.223539431939585	22.865783932556802
28-29	23.071823578627708	26.963213614611288	27.13140605961356	22.833556747147444
30-31	23.364256606175637	26.681245631587995	27.060875568372012	22.893622193864356
32-33	23.11722108050791	26.75884511556987	26.90466596563165	23.21926783829057
34-35	23.40429381634495	26.7890650136155	27.064645710625886	22.741995459413666
36-37	23.420285081742684	26.668769620969545	27.057878018599546	22.853067278688222
38-39	23.309996817747873	26.777839835302213	26.96408972281007	22.94807362413984
40-41	23.40152073421666	26.661419876150696	27.00930389099851	22.92775549863414
42-43	23.29728809407608	26.609440268110145	26.876334937268386	23.21693670054539
44-45	23.314999838786488	26.701550642035716	26.89027483540564	23.093174683772155
46-47	23.48704951546011	26.68231965830642	26.619967492438523	23.210663333794944
48-49	23.21446259031406	26.8278832706361	26.708628233032833	23.24902590601701
50-51	23.331963250862408	26.84794125779138	26.662585800254966	23.157509691091246
52-53	23.57252236308568	26.67178649080821	26.633097877089895	23.12259326901622
54-55	23.184334347545132	26.673229122645715	26.840483611565073	23.301952918244076
56-57	23.254194042227468	26.756673064767728	26.667810979605328	23.32132191339948
58-59	23.292550396830457	26.644599266765407	26.760464714389933	23.302385622014203
60-61	23.29301516425527	26.756426686769696	26.807712604018676	23.142845544956366
62-63	23.305782171446655	26.667017735388676	26.794469600503128	23.232730492661542
64-65	23.080337358902074	26.53562106288936	26.839174468381305	23.544867109827262
66-67	23.101889363155514	26.680258906885037	26.683994568672603	23.53385716128685
68-69	23.212829062454468	26.66019530999871	26.70439201573171	23.422583611815114
70-71	23.151422871285153	26.496044008978885	26.66306391260947	23.689469207126493
72-73	23.14332207504525	26.645370421496757	26.50860518951555	23.702702313942446
74-75	23.06780547728778	26.750341329986348	26.54446453822142	23.637388654504456
76-77	23.114160060544975	26.41307064626645	26.68150065043996	23.791268642748612
78-79	23.09178652017638	26.447909157205814	26.68056801813365	23.77973630448416
80-81	23.21139272672842	26.532205942588472	26.51338515432002	23.743016176363085
82-83	23.205976000881552	26.602738613758024	26.531309628065863	23.65997575729456
84-85	23.163928846449664	26.57761761497928	26.49464891315873	23.76380462541232
86-87	23.04713877792343	26.641142998544304	26.4686226249142	23.84309559861807
88-89	23.020492496224797	26.50091202573906	26.479934154602258	23.998661323433886
90-91	22.964646969989598	26.457995443873312	26.571433225286274	24.00592436085082
92-93	22.990738875651047	26.348325766569914	26.67430304864295	23.986632309136088
94-95	22.917482856817458	26.29332650459073	26.63564506218586	24.153545576405953
96-97	22.85628259553916	26.14186886928984	26.742265855497855	24.25958267967314
98-99	22.7154013083611	26.11512686983282	26.62359893007941	24.545872891726674
100-101	22.716062383114004	26.133778235586508	26.66214991607055	24.488009465228945
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	38.5
2	36.0
3	55.0
4	60.5
5	64.0
6	115.0
7	268.0
8	415.5
9	435.5
10	378.0
11	314.5
12	304.0
13	325.5
14	361.5
15	412.5
16	462.0
17	553.0
18	649.0
19	706.0
20	820.0
21	977.0
22	1168.5
23	1459.5
24	1840.0
25	2428.5
26	3367.5
27	4731.0
28	6659.5
29	9430.0
30	13037.0
31	17829.5
32	24110.5
33	29726.5
34	34812.0
35	40725.0
36	47869.5
37	54728.0
38	60518.0
39	65841.5
40	69411.5
41	73045.5
42	77505.0
43	82044.0
44	85988.5
45	90118.0
46	92646.0
47	93030.5
48	93918.5
49	93614.5
50	91686.5
51	88760.0
52	85711.5
53	82735.0
54	79119.5
55	73989.5
56	67299.0
57	59457.0
58	51953.0
59	44706.0
60	36370.5
61	29301.5
62	23618.0
63	17623.5
64	12208.0
65	8571.5
66	6275.0
67	4668.5
68	3480.5
69	2604.0
70	1923.5
71	1360.5
72	934.5
73	656.0
74	484.0
75	317.5
76	194.5
77	120.5
78	69.5
79	44.5
80	26.5
81	21.5
82	13.0
83	10.0
84	7.5
85	1.5
86	1.5
87	2.5
88	2.5
89	2.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	1.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1541949229790793
2	2.7833018588281465E-4
3	0.006494371003932342
4	0.0048243898886354535
5	0.029178281153381735
6	0.0012988742007864684
7	1.3916509294140732E-4
8	0.002319418215690122
9	0.0
10-11	0.10750503429723715
12-13	3.7110691451041955E-4
14-15	0.0057057688105977
16-17	4.1749527882422194E-4
18-19	4.638836431380244E-4
20-21	0.001043738197060555
22-23	2.0874763941211097E-4
24-25	0.0017627578439244928
26-27	0.0028760785874557514
28-29	0.006795895371972058
30-31	0.015053024219828891
32-33	0.0152385776770841
34-35	0.012269722361000745
36-37	7.190196468639378E-4
38-39	3.247185501966171E-4
40-41	0.005079525892361368
42-43	0.00839629394079824
44-45	0.008651429944524155
46-47	0.009950304145310623
48-49	0.011527508531979907
50-51	0.005798545539225305
52-53	0.0018555345725520976
54-55	7.422138290208391E-4
56-57	0.005821739721382207
58-59	0.009068925223348377
60-61	0.006981448829227267
62-63	0.01806826790022605
64-65	0.0342346128635862
66-67	0.037435410001238574
68-69	0.0266733094804364
70-71	0.040821760596146145
72-73	0.025977484015729368
74-75	0.02507291091161022
76-77	0.2433301650080507
78-79	0.06893310937031043
80-81	0.054761464072443776
82-83	0.019738249015522936
84-85	0.03170644700848397
86-87	0.0818522688317044
88-89	0.2703745814029975
90-91	0.3227702388954374
92-93	0.05172302620988972
94-95	0.005497021171185589
96-97	0.010321411059821043
98-99	0.001809146208238295
100-101	0.0015540102045123816
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2155713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.76956463685252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24709796099071	52.62339162941076
2	10.957490737372762	14.194238091439557
3	3.292576765236557	6.39776290853364
4	1.436811151487954	3.7224653098899814
5	0.7933785665252572	2.569339217302552
6	0.5051249473023353	1.96300337543912
7	0.3339936011521699	1.5142834096684428
8	0.23551784679016097	1.220351072064613
9	0.18765146428697202	1.0938693280801552
>10	0.9500727454337046	10.73576455165306
>50	0.04617402145877435	2.031967916668713
>100	0.013175517755830769	1.4501404508470261
>500	7.189801590683242E-4	0.3055794252319286
>1k	2.156940477204847E-4	0.17784331377029533
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.6388364313802443E-5	0.0
3	0.0	0.0	0.0	4.6388364313802443E-5	0.0
4	0.0	0.0	0.0	4.6388364313802443E-5	0.0
5	0.0	0.0	0.0	4.6388364313802443E-5	0.0
6	0.0	0.0	0.0	4.6388364313802443E-5	4.6388364313802443E-5
7	0.0	0.0	0.0	4.6388364313802443E-5	4.6388364313802443E-5
8	0.0	0.0	0.0	4.6388364313802443E-5	4.6388364313802443E-5
9	0.0	0.0	0.0	4.6388364313802443E-5	4.6388364313802443E-5
10-11	0.0	0.0	0.0	1.6235927509830855E-4	4.6388364313802443E-5
12-13	0.0	0.0	0.0	1.8555345725520977E-4	1.159709107845061E-4
14-15	0.0	0.0	0.0	1.8555345725520977E-4	1.8555345725520975E-4
16-17	0.0	0.0	0.0	1.8555345725520977E-4	2.319418215690122E-4
18-19	0.0	0.0	0.0	1.8555345725520977E-4	2.319418215690122E-4
20-21	0.0	0.0	0.0	1.8555345725520977E-4	2.319418215690122E-4
22-23	0.0	0.0	0.0	3.0152436803971585E-4	2.319418215690122E-4
24-25	0.0	0.0	0.0	3.7110691451041955E-4	2.319418215690122E-4
26-27	0.0	0.0	0.0	5.798545539225305E-4	2.319418215690122E-4
28-29	6.958254647070366E-5	0.0	0.0	0.0020642822119642085	2.319418215690122E-4
30-31	9.277672862760489E-5	0.0	0.0	0.007190196468639378	2.319418215690122E-4
32-33	9.277672862760489E-5	0.0	0.0	0.018671316636305484	2.319418215690122E-4
34-35	1.3916509294140732E-4	0.0	0.0	0.03390989431338958	2.319418215690122E-4
36-37	1.3916509294140732E-4	0.0	0.0	0.05949307723245163	2.319418215690122E-4
38-39	1.3916509294140732E-4	0.0	0.0	0.1044202080703693	2.319418215690122E-4
40-41	1.3916509294140732E-4	0.0	0.0	0.15426450552555002	2.319418215690122E-4
42-43	1.3916509294140732E-4	0.0	0.0	0.20837653249760058	2.319418215690122E-4
44-45	1.3916509294140732E-4	0.0	0.0	0.2722533101577065	2.5513600372591345E-4
46-47	1.3916509294140732E-4	0.0	0.0	0.34879411127548055	2.7833018588281465E-4
48-49	1.3916509294140732E-4	0.0	0.0	0.4321308077652266	2.7833018588281465E-4
50-51	1.3916509294140732E-4	0.0	0.0	0.5172070679167403	2.7833018588281465E-4
52-53	1.3916509294140732E-4	0.0	0.0	0.6077571550572827	3.0152436803971585E-4
54-55	1.3916509294140732E-4	0.0	0.0	0.7105769645588258	3.4791273235351835E-4
56-57	1.3916509294140732E-4	0.0	0.0	0.830769216495888	3.9430109666732075E-4
58-59	1.3916509294140732E-4	0.0	0.0	0.9615612096786539	4.1749527882422194E-4
60-61	1.8555345725520977E-4	0.0	0.0	1.1039270997577137	4.1749527882422194E-4
62-63	1.8555345725520977E-4	0.0	0.0	1.2493314276993273	4.1749527882422194E-4
64-65	1.8555345725520977E-4	0.0	0.0	1.415169830121171	4.1749527882422194E-4
66-67	1.8555345725520977E-4	0.0	0.0	1.603019511409914	4.1749527882422194E-4
68-69	1.8555345725520977E-4	0.0	0.0	1.8087982955059416	4.1749527882422194E-4
70-71	1.8555345725520977E-4	0.0	0.0	2.029699686368269	4.1749527882422194E-4
72-73	1.8555345725520977E-4	0.0	0.0	2.2575825260598235	5.102720074518268E-4
74-75	2.08747639412111E-4	0.0	0.0	2.4993818750455183	5.102720074518268E-4
76-77	2.319418215690122E-4	0.0	0.0	2.7762972158167623	5.102720074518268E-4
78-79	2.319418215690122E-4	0.0	0.0	3.0814862646372685	5.102720074518268E-4
80-81	2.319418215690122E-4	0.0	0.0	3.41687413862606	5.102720074518268E-4
82-83	2.319418215690122E-4	0.0	0.0	3.7651579778940887	5.102720074518268E-4
84-85	2.319418215690122E-4	0.0	0.0	4.1315332792445005	5.33466189608728E-4
86-87	2.319418215690122E-4	0.0	0.0	4.531934445819086	6.262429182363329E-4
88-89	2.319418215690122E-4	0.0	0.0	4.963137486298037	6.958254647070366E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2010	0.0	50.365166	1
GTATCAA	3530	0.0	41.065	1
TCAACGC	4530	0.0	30.693077	4
CAACGCA	4580	0.0	30.308538	5
ATCAACG	4740	0.0	29.533485	3
TATCAAC	4855	0.0	28.83393	2
AACGCAG	4845	0.0	28.74691	6
ACGCAGA	5720	0.0	23.933918	7
GTACATG	5550	0.0	23.549755	1
CGCAGAG	5880	0.0	22.635916	8
TACATGG	5805	0.0	22.316774	2
ACATGGG	5695	0.0	22.081224	3
CATGGGG	4705	0.0	19.667358	4
AGAGTAC	5715	0.0	19.421734	10-11
GCAGAGT	6630	0.0	18.856434	9
GTACTAC	545	0.0	17.441353	1
GTACACT	1665	0.0	16.815462	4
CAGAGTA	6355	0.0	16.680407	9
TACACTG	2420	0.0	16.501049	5
TAAGGTG	1045	0.0	16.376982	5
>>END_MODULE