FastQCFastQC Report
Mon 15 Aug 2016
SRR2921392_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921392_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738867
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14850.049.2062151
GTATCAA27600.044.870111
TCAACGC33450.035.323724
CAACGCA34400.034.6221055
ATCAACG35200.033.4317973
AACGCAG35950.032.8549776
TATCAAC36850.032.0654642
GTACATG46750.027.099131
ACGCAGA43100.026.6387467
CGCAGAG44350.025.889438
TACATGG47900.025.0645942
ACATGGG49100.023.8707563
CATGGGG40850.022.3034674
TATACTG8000.021.3525965
GCAGAGT51050.020.9110099
CGTAGTG1605.0316456E-520.759475
TATACCG1605.0316456E-520.759475
ATACCGT1856.633152E-620.5125186
GTATAGG5200.020.0745121
AGAGTAC44200.019.75104310-11
Produced by FastQC (version 0.11.5)