##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921392_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1738867
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.40559283717501	31.0	31.0	34.0	26.0	34.0
2	30.484252677174275	33.0	31.0	34.0	26.0	34.0
3	30.52498839761753	33.0	31.0	34.0	26.0	34.0
4	33.98396829659773	37.0	35.0	37.0	30.0	37.0
5	33.98514837535016	37.0	35.0	37.0	30.0	37.0
6	34.0301713702083	37.0	35.0	37.0	30.0	37.0
7	33.94552544846731	37.0	35.0	37.0	30.0	37.0
8	33.91303877754883	37.0	35.0	37.0	30.0	37.0
9	35.47408283669769	39.0	37.0	39.0	30.0	39.0
10-11	35.36724545350507	39.0	36.0	39.0	29.5	39.0
12-13	35.26171840629559	39.0	35.0	39.0	29.0	39.0
14-15	36.395916421439935	40.0	37.0	41.0	28.5	41.0
16-17	36.35465765926894	40.0	37.0	41.0	28.0	41.0
18-19	36.313308895965015	40.0	37.0	41.0	28.0	41.0
20-21	36.261028876849124	40.0	37.0	41.0	27.0	41.0
22-23	36.143947179399	40.0	36.5	41.0	27.0	41.0
24-25	36.05643646121296	40.0	36.0	41.0	27.0	41.0
26-27	35.91269372528204	40.0	36.0	41.0	27.0	41.0
28-29	35.774935633375065	40.0	36.0	41.0	27.0	41.0
30-31	35.62388181499793	40.0	36.0	41.0	26.0	41.0
32-33	35.44056675984995	39.5	35.0	41.0	25.0	41.0
34-35	35.278964693676976	39.0	35.0	41.0	25.0	41.0
36-37	35.1128867245166	39.0	35.0	41.0	24.0	41.0
38-39	35.32651548393292	39.5	35.0	41.0	25.0	41.0
40-41	35.43815484450507	40.0	35.0	41.0	25.0	41.0
42-43	35.272014765936675	40.0	35.0	41.0	24.0	41.0
44-45	35.14770479858437	40.0	35.0	41.0	23.5	41.0
46-47	34.98369052952296	40.0	35.0	41.0	23.0	41.0
48-49	34.83952079141188	40.0	35.0	41.0	22.0	41.0
50-51	33.14808176818583	37.0	32.5	39.5	18.0	40.0
52-53	33.31159398619906	37.5	33.5	39.5	16.5	40.0
54-55	34.181180331790756	39.0	34.0	40.5	18.5	41.0
56-57	34.21223848632472	39.0	34.0	41.0	18.0	41.0
58-59	34.050522265360144	39.0	34.0	41.0	17.5	41.0
60-61	33.82648644203381	38.0	34.0	41.0	14.5	41.0
62-63	33.55384885675558	38.0	34.0	40.0	12.5	41.0
64-65	33.27377453249731	37.0	34.0	40.0	9.5	41.0
66-67	32.94578136223184	37.0	33.0	40.0	7.5	41.0
68-69	32.586351342569614	36.0	33.0	39.0	6.0	41.0
70-71	32.20739481512962	36.0	33.0	39.0	2.0	41.0
72-73	31.85053054661455	35.0	32.0	38.5	2.0	40.0
74-75	31.444925057523086	35.0	32.0	37.0	2.0	39.5
76-77	31.061956722394527	35.0	32.0	37.0	2.0	39.0
78-79	30.71212174364112	35.0	31.5	36.5	2.0	39.0
80-81	30.349404238507027	35.0	31.0	36.0	2.0	37.5
82-83	30.03075853414896	35.0	31.0	35.5	2.0	37.0
84-85	29.780622094731797	35.0	31.0	35.0	2.0	37.0
86-87	29.54520702273377	35.0	31.0	35.0	2.0	36.0
88-89	29.333668704967085	35.0	31.0	35.0	2.0	36.0
90-91	29.077016816122224	35.0	30.0	35.0	2.0	36.0
92-93	28.88206544836379	34.5	30.0	35.0	2.0	35.5
94-95	28.679148549026465	34.0	30.0	35.0	2.0	35.0
96-97	28.4306502452459	34.0	30.0	35.0	2.0	35.0
98-99	28.207740442483527	34.0	30.0	35.0	2.0	35.0
100-101	27.178240774021244	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59040.0
3	10107.0
4	6753.0
5	5209.0
6	4791.0
7	4770.0
8	5127.0
9	5505.0
10	5977.0
11	6602.0
12	6635.0
13	6763.0
14	7449.0
15	8280.0
16	8848.0
17	9549.0
18	8381.0
19	7242.0
20	7390.0
21	7706.0
22	8384.0
23	9543.0
24	10927.0
25	12812.0
26	15380.0
27	17669.0
28	21243.0
29	25166.0
30	29871.0
31	36579.0
32	45625.0
33	58495.0
34	80888.0
35	116774.0
36	193181.0
37	356829.0
38	418044.0
39	89314.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.78111355847551	19.740333869605344	13.889387568156032	24.58916500376311
2	15.41048376933705	19.943192248089673	40.353299102135985	24.29302488043729
3	18.65300298054018	24.13524057217165	30.377746067182986	26.834010380105184
4	12.853928705009062	15.888080098972868	37.551112006214574	33.70687918980349
5	13.599442572223088	37.73868904356372	35.07888166915327	13.582986715059928
6	32.83193445519679	36.15726105182752	17.290870019372623	13.719934473603077
7	28.119796408914617	32.32902887006258	21.559511395199422	17.99166332582339
8	26.076184986991503	35.8092332435422	20.004038012538548	18.11054375692775
9	26.49459327471134	15.538547112626352	19.764817947601443	38.20204166506086
10-11	25.219629190858594	25.601868110028438	28.500786480304342	20.677716218808627
12-13	25.598776775649874	23.04397860853053	28.374875924472338	22.982368691347258
14-15	22.804660078208812	24.79290603004132	25.538976530740527	26.863457361009345
16-17	22.883791808794204	27.97182099567081	26.32623364400766	22.818153551527327
18-19	22.934459946658627	27.320900435480265	27.656750318032035	22.08788929982907
20-21	23.350429887445767	26.470723589217897	27.800711244263276	22.378135279073057
22-23	23.261021101608144	26.235223278771436	27.41661298892891	23.087142630691506
24-25	23.054349395794986	26.39035044063268	27.47388049611379	23.08141966745854
26-27	22.88296819080569	26.63244108657396	27.446928585933954	23.037662136686397
28-29	22.970072011472137	26.898174432389805	27.276006012172754	22.855747543965308
30-31	23.437176466798878	26.555317562935116	27.120796081480904	22.886709888785102
32-33	23.12546784760052	26.586224438590065	27.140351548199916	23.147956165609497
34-35	23.381618950446374	26.696802520529918	27.203393144922416	22.718185384101293
36-37	23.32176723003855	26.522511631999507	27.20848402000063	22.947237117961315
38-39	23.160577867063427	26.669155060075145	27.175067678357706	22.995199394503718
40-41	23.30812062990351	26.59905705409344	27.15400682997413	22.93881548602892
42-43	23.130525652631146	26.538695311223375	27.048113759745046	23.282665276400433
44-45	23.05696496126175	26.631890121098444	27.150858753057715	23.160286164582093
46-47	23.209381651562776	26.584755767076913	26.88540244268941	23.3204601386709
48-49	23.022623125400507	26.767788237680545	26.86284362855154	23.34674500836741
50-51	23.124619489787126	26.768239783165725	26.929159767180334	23.177980959866815
52-53	23.375982351812333	26.59243716607818	26.898790893986973	23.132789588122513
54-55	22.96297299446658	26.477576451903275	27.1998831308485	23.359567422781645
56-57	23.0472971220596	26.641077289716126	26.92276215423363	23.388863433990643
58-59	23.151798177213905	26.594217431847234	27.021674256806055	23.232310134132806
60-61	23.212561284415884	26.641174539803135	27.090413479070147	23.055850696710838
62-63	23.113187701752455	26.596052467020804	27.14250228659986	23.14825754462688
64-65	22.88456584171569	26.43426029613398	27.15136883152661	23.52980503062372
66-67	22.973435946286966	26.506079641736374	26.963302585753073	23.557181826223587
68-69	22.939117466039985	26.521198933195638	27.07459793139535	23.465085669369024
70-71	22.924529171597836	26.32625933561425	27.064350485549895	23.684861007238016
72-73	22.919583194919788	26.51639541453332	26.93516541487041	23.628855975676476
74-75	22.89348377823255	26.62166534470842	26.96305037856601	23.521800498493025
76-77	22.881982688754547	26.327377472793263	26.93522731561605	23.855412522836144
78-79	22.84090083838327	26.390052829788484	26.96647734935787	23.80256898247038
80-81	22.97383877519435	26.453038712249004	26.803273042418645	23.769849470138
82-83	23.010313509238813	26.399252064517505	26.970780600019946	23.61965382622374
84-85	22.955532686433695	26.461799424608795	26.873414799536715	23.709253089420795
86-87	22.817601462868527	26.587560544517295	26.860781910606836	23.734056082007342
88-89	22.753871403066917	26.310149206753646	26.954494332472283	23.981485057707154
90-91	22.880900622639736	26.193267927625985	26.998474097414498	23.92735735231978
92-93	22.926360627896297	26.110260314835937	27.05215088120107	23.911228176066693
94-95	22.895372275163513	26.057877694855215	27.049565963413624	23.99718406656765
96-97	22.764877561657357	25.853251569397102	27.119032024464605	24.262838844480935
98-99	22.54950765462233	25.88686591850424	27.1404650798121	24.423161347061335
100-101	22.787029149387912	25.783781680122114	27.23221351137479	24.196975659115182
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	57.0
2	61.0
3	91.0
4	90.0
5	99.5
6	161.5
7	309.0
8	480.0
9	537.0
10	503.0
11	443.0
12	449.5
13	534.0
14	647.5
15	724.5
16	763.5
17	844.5
18	977.0
19	1103.0
20	1202.5
21	1384.0
22	1605.5
23	1852.5
24	2279.0
25	2981.0
26	4065.0
27	5554.5
28	7444.0
29	10022.5
30	13343.5
31	17096.0
32	21821.0
33	25979.5
34	29285.0
35	33107.0
36	37990.5
37	42870.0
38	47121.0
39	50667.5
40	53254.0
41	56355.5
42	59138.0
43	61963.5
44	65066.0
45	68130.0
46	70323.0
47	71585.5
48	72981.0
49	73503.0
50	72554.0
51	71020.5
52	69036.5
53	66929.5
54	64433.0
55	60181.5
56	54851.0
57	48850.0
58	42838.5
59	36641.5
60	30220.0
61	24959.5
62	20246.0
63	15182.5
64	10858.5
65	7885.0
66	5750.0
67	4316.0
68	3352.0
69	2522.0
70	1900.0
71	1438.5
72	994.5
73	728.5
74	581.0
75	442.0
76	319.0
77	222.0
78	151.5
79	102.0
80	73.0
81	59.5
82	52.5
83	48.0
84	38.5
85	26.5
86	28.0
87	22.0
88	16.0
89	19.0
90	17.0
91	12.0
92	8.5
93	8.5
94	10.0
95	9.0
96	6.0
97	7.0
98	7.5
99	5.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05423071459749366
2	0.061879373178052144
3	0.05331057521938136
4	0.058486359221263044
5	0.05083770064070455
6	0.019035383384698196
7	0.016332473961493316
8	0.022313379919223265
9	0.02208334507469519
10-11	0.024469956586673967
12-13	0.02935819703289556
14-15	0.04758845846174549
16-17	0.031716054189308326
18-19	0.02539009596478627
20-21	0.029041899121669452
22-23	0.050118841751554316
24-25	0.045891951483350936
26-27	0.03427519183468316
28-29	0.022658432186015376
30-31	0.014434686494136699
32-33	0.010725374626121493
34-35	0.010639111559423464
36-37	0.0012364372893384026
38-39	0.007361115024898396
40-41	0.003508031379053142
42-43	0.22701563719364393
44-45	0.20139550638432957
46-47	0.20746267540875754
48-49	0.20490353776338271
50-51	0.20314952207385614
52-53	0.20806651687564373
54-55	0.20662879909734327
56-57	0.21692285838997463
58-59	0.2139899141222417
60-61	0.21976953959100953
62-63	0.21611773643412635
64-65	0.21703787581223866
66-67	0.20752018411988957
68-69	0.20651378167507922
70-71	0.19029632513584996
72-73	0.19711110740499418
74-75	0.19762868580518234
76-77	0.19584591576008978
78-79	0.22204113368072428
80-81	0.23167384279533743
82-83	0.2276194786605301
84-85	0.22275999256987453
86-87	0.24113402577655452
88-89	0.2392362383091979
90-91	0.2390062034646698
92-93	0.24254298919928896
94-95	0.25597127324861535
96-97	0.2555112035595592
98-99	0.2450733724890978
100-101	0.25530992307059713
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1738867.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.31968098893688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.8183119709541	56.58120653897837
2	10.149750558013029	13.868554404814684
3	2.9615636100199882	6.069992431950295
4	1.343356861947709	3.6711084905026716
5	0.7462429261652576	2.549153932793059
6	0.4599501836659391	1.8854189891315936
7	0.3123127935419399	1.4935977296484328
8	0.22074496381883266	1.206498040641365
9	0.1612931569851092	0.9917547327829059
>10	0.7930606384093051	9.176137665041523
>50	0.022605621973318984	1.0148091791818263
>100	0.010384737356895882	1.2125268367926993
>500	2.531862891985131E-4	0.12039134130135332
>1k	1.6879085946567535E-4	0.1588496864392856
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	5.750871113201872E-5
12-13	0.0	0.0	0.0	5.750871113201872E-5	1.1501742226403744E-4
14-15	0.0	0.0	0.0	5.750871113201872E-5	1.437717778300468E-4
16-17	0.0	0.0	0.0	8.626306669802808E-5	1.7252613339605616E-4
18-19	0.0	0.0	0.0	1.1501742226403744E-4	2.300348445280749E-4
20-21	0.0	0.0	0.0	1.437717778300468E-4	2.5878920009408425E-4
22-23	0.0	0.0	0.0	2.5878920009408425E-4	3.162979112261029E-4
24-25	0.0	0.0	0.0	6.901045335842247E-4	3.450522667921123E-4
26-27	0.0	0.0	0.0	9.201393781122995E-4	3.450522667921123E-4
28-29	0.0	0.0	0.0	0.0026166463565068516	3.450522667921123E-4
30-31	0.0	0.0	0.0	0.011012918181781586	3.450522667921123E-4
32-33	0.0	0.0	0.0	0.028150514099123163	4.0256097792413105E-4
34-35	0.0	0.0	0.0	0.05069392886287451	4.0256097792413105E-4
36-37	0.0	0.0	0.0	0.08879344998783692	4.0256097792413105E-4
38-39	0.0	0.0	0.0	0.14555454787513938	4.0256097792413105E-4
40-41	0.0	0.0	0.0	0.21059690016545257	4.0256097792413105E-4
42-43	0.0	0.0	0.0	0.27871596850132874	4.0256097792413105E-4
44-45	0.0	0.0	0.0	0.36469149164369674	4.313153334901404E-4
46-47	0.0	0.0	0.0	0.46691322568086	4.888240446221592E-4
48-49	0.0	0.0	0.0	0.5755471810092434	5.175784001881685E-4
50-51	0.0	0.0	0.0	0.6834047687373445	5.175784001881685E-4
52-53	0.0	0.0	0.0	0.7948566509111967	5.750871113201872E-4
54-55	0.0	0.0	0.0	0.9296570698046487	6.613501780182153E-4
56-57	0.0	0.0	0.0	1.0841254679052508	6.901045335842246E-4
58-59	0.0	0.0	0.0	1.246673839919902	6.901045335842246E-4
60-61	0.0	5.750871113201872E-5	0.0	1.4183660970045437	6.901045335842246E-4
62-63	0.0	5.750871113201872E-5	0.0	1.5939401920905971	6.901045335842246E-4
64-65	0.0	5.750871113201872E-5	0.0	1.7907350015843648	6.901045335842246E-4
66-67	0.0	5.750871113201872E-5	0.0	2.0138975551321634	6.901045335842246E-4
68-69	0.0	5.750871113201872E-5	0.0	2.259057190687959	6.901045335842246E-4
70-71	0.0	5.750871113201872E-5	0.0	2.5150572182921405	6.901045335842246E-4
72-73	0.0	5.750871113201872E-5	0.0	2.780258639677445	7.476132447162434E-4
74-75	0.0	5.750871113201872E-5	0.0	3.062942709246883	7.476132447162434E-4
76-77	0.0	5.750871113201872E-5	0.0	3.3744386430934625	7.476132447162434E-4
78-79	0.0	5.750871113201872E-5	0.0	3.7263344465102852	7.763676002822528E-4
80-81	0.0	5.750871113201872E-5	0.0	4.091601025265302	8.338763114142714E-4
82-83	0.0	5.750871113201872E-5	0.0	4.478232090205864	8.626306669802809E-4
84-85	0.0	5.750871113201872E-5	0.0	4.879470367773958	8.626306669802809E-4
86-87	0.0	5.750871113201872E-5	0.0	5.315127608954566	8.626306669802809E-4
88-89	0.0	5.750871113201872E-5	0.0	5.789747001927116	8.626306669802809E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1485	0.0	49.206215	1
GTATCAA	2760	0.0	44.87011	1
TCAACGC	3345	0.0	35.32372	4
CAACGCA	3440	0.0	34.622105	5
ATCAACG	3520	0.0	33.431797	3
AACGCAG	3595	0.0	32.854977	6
TATCAAC	3685	0.0	32.065464	2
GTACATG	4675	0.0	27.09913	1
ACGCAGA	4310	0.0	26.638746	7
CGCAGAG	4435	0.0	25.88943	8
TACATGG	4790	0.0	25.064594	2
ACATGGG	4910	0.0	23.870756	3
CATGGGG	4085	0.0	22.303467	4
TATACTG	800	0.0	21.352596	5
GCAGAGT	5105	0.0	20.911009	9
CGTAGTG	160	5.0316456E-5	20.75947	5
TATACCG	160	5.0316456E-5	20.75947	5
ATACCGT	185	6.633152E-6	20.512518	6
GTATAGG	520	0.0	20.074512	1
AGAGTAC	4420	0.0	19.751043	10-11
>>END_MODULE