FastQCFastQC Report
Mon 15 Aug 2016
SRR2921392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738867
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.055.671291
GTATCAA28100.038.4092831
TCAACGC35150.029.8432334
ATCAACG36850.028.5952823
CAACGCA37150.028.2903125
TATCAAC38800.027.769822
AACGCAG39050.027.0324956
GTACATG45500.026.9603161
TACATGG48450.025.0798592
ACATGGG49600.024.211283
CATGGGG40800.023.2674474
TAGATCG1050.002181709722.645665
ACGCAGA45600.022.41967
CGCAGAG47200.021.3573848
TACACTG19750.019.7447225
ATGGGGG26500.019.3812685
AGAGTAC45750.019.17606710-11
TATACTG8100.018.2004015
GCAGAGT52950.017.9604919
GTACACT14750.017.6990344