##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921392_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1738867 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.543586714797623 33.0 31.0 34.0 30.0 34.0 2 31.676580209987307 34.0 31.0 34.0 30.0 34.0 3 31.75034778393057 34.0 31.0 34.0 30.0 34.0 4 35.23904070869135 37.0 35.0 37.0 33.0 37.0 5 35.000478472476615 37.0 35.0 37.0 32.0 37.0 6 34.96366542121968 37.0 35.0 37.0 32.0 37.0 7 34.83670861543752 37.0 35.0 37.0 32.0 37.0 8 34.804247823439056 37.0 35.0 37.0 32.0 37.0 9 36.44335363199141 39.0 37.0 39.0 32.0 39.0 10-11 36.2956088648528 39.0 37.0 39.0 32.0 39.0 12-13 36.239825127511196 39.0 37.0 39.0 32.0 39.0 14-15 37.50192194112603 40.0 38.0 41.0 32.0 41.0 16-17 37.48779665149779 40.0 38.0 41.0 32.0 41.0 18-19 37.46337586485913 40.0 38.0 41.0 32.0 41.0 20-21 37.43538925058673 40.0 38.0 41.0 32.0 41.0 22-23 37.325356108316505 40.0 38.0 41.0 31.5 41.0 24-25 37.26058030890229 40.0 38.0 41.0 31.0 41.0 26-27 37.13361228892147 40.0 38.0 41.0 31.0 41.0 28-29 36.98577608293216 40.0 37.0 41.0 30.5 41.0 30-31 36.846239246589874 40.0 37.0 41.0 30.0 41.0 32-33 36.791642489046026 40.0 37.0 41.0 30.0 41.0 34-35 36.916979849522704 40.0 37.0 41.0 30.0 41.0 36-37 36.88794686425126 40.0 37.0 41.0 30.0 41.0 38-39 36.800117260262 40.0 37.0 41.0 30.0 41.0 40-41 36.6777683399593 40.0 37.0 41.0 30.0 41.0 42-43 36.58103494976902 40.0 37.0 41.0 30.0 41.0 44-45 36.44044771681791 40.0 37.0 41.0 30.0 41.0 46-47 36.27123696061861 40.0 36.0 41.0 29.0 41.0 48-49 36.11274956624055 40.0 36.0 41.0 28.5 41.0 50-51 35.969240315676814 40.0 36.0 41.0 28.0 41.0 52-53 35.81007173061539 40.0 35.0 41.0 28.0 41.0 54-55 35.61317053000603 39.0 35.0 41.0 27.5 41.0 56-57 35.3657381501863 39.0 35.0 41.0 27.0 41.0 58-59 35.15521687397599 39.0 35.0 41.0 26.0 41.0 60-61 34.897153433816385 38.5 35.0 40.5 26.0 41.0 62-63 34.61415019090016 38.0 34.5 40.0 26.0 41.0 64-65 34.31131794438563 37.0 34.0 40.0 26.0 41.0 66-67 33.97935926094405 37.0 34.0 40.0 25.5 41.0 68-69 33.62293320880781 36.0 34.0 39.0 25.0 41.0 70-71 33.24221518954583 36.0 34.0 39.0 23.5 40.5 72-73 32.839270340974906 35.0 33.5 38.5 22.5 40.0 74-75 32.42413134529553 35.0 33.0 37.0 21.5 39.5 76-77 29.64159708591859 33.0 29.0 34.5 16.5 37.0 78-79 31.301119349553474 34.5 31.5 36.0 19.5 38.5 80-81 31.484211558445814 35.0 32.5 36.0 20.0 37.5 82-83 31.302856975260326 35.0 33.0 35.5 20.0 37.0 84-85 31.05571098882203 35.0 32.5 35.0 18.5 37.0 86-87 30.79629465623305 35.0 32.0 35.0 17.0 36.0 88-89 30.507364565547565 35.0 32.0 35.0 10.0 36.0 90-91 30.31676660722183 35.0 32.0 35.0 4.5 36.0 92-93 30.164438970893116 35.0 32.0 35.0 3.5 35.0 94-95 30.06355287667199 34.5 32.0 35.0 2.0 35.0 96-97 29.878463102698483 34.0 32.0 35.0 2.0 35.0 98-99 29.621824153313625 34.0 31.0 35.0 2.0 35.0 100-101 28.545572490593013 33.5 29.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28070.0 3 9789.0 4 5379.0 5 3607.0 6 3265.0 7 3285.0 8 3471.0 9 3791.0 10 4260.0 11 4680.0 12 5038.0 13 5691.0 14 6190.0 15 6977.0 16 7925.0 17 9062.0 18 8241.0 19 6832.0 20 6388.0 21 6364.0 22 6516.0 23 7300.0 24 8455.0 25 9993.0 26 12172.0 27 14714.0 28 17616.0 29 21215.0 30 25820.0 31 32126.0 32 40769.0 33 53749.0 34 77371.0 35 115062.0 36 199868.0 37 404026.0 38 478096.0 39 75690.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.49518130515751 20.756475472206752 13.997054234005798 24.751288988629945 2 15.123900355233353 21.318774344296283 38.45227065825424 25.105054642216125 3 17.58190312359991 25.12113719718576 30.152795343270466 27.14416433594386 4 12.793752451411725 17.100703131020694 36.50909768494715 33.59644673262043 5 13.53706339244316 38.068313212674575 35.02507556033149 13.369547834550774 6 32.94717706618236 36.64606489242585 16.901314491729288 13.505443549662507 7 28.129498264672648 32.61357264652517 21.36911146063668 17.887817628165497 8 26.36111325175376 36.094244764020026 19.712807548046566 17.831834436179648 9 26.596226163358093 15.451037945972868 19.604604607482916 38.34813128318612 10-11 25.286516908916205 25.869452953770995 28.281508800028554 20.562521337284245 12-13 25.635608755023753 23.261128167260875 28.296109328817742 22.80715374889763 14-15 22.82801141841975 24.981517101605473 25.41515709816626 26.775314381808514 16-17 23.015105547327007 28.141496936650324 26.231169823755675 22.612227692266995 18-19 23.04811334996298 27.490586518485504 27.58193934252996 21.87936078902156 20-21 23.44334256930139 26.590616578505156 27.707242816245643 22.258798035947812 22-23 23.359402092056705 26.312624973905365 27.403826523423643 22.924146410614295 24-25 23.13978341264403 26.58205644981572 27.408816182545053 22.869343954995195 26-27 22.972345022684937 26.805320162708135 27.421427992217705 22.80090682238922 28-29 23.080473374822933 27.021151414033884 27.22977842115222 22.668596789990964 30-31 23.47754714202888 26.683705515318284 27.110721648061403 22.728025694591437 32-33 23.21382351283293 26.77731865310306 27.047942739279435 22.960915094784575 34-35 23.422476225095266 26.8344615550969 27.169750332226055 22.573311887581777 36-37 23.382946383745185 26.699464073238914 27.29114585040633 22.62644369260957 38-39 23.315049365562803 26.840914528105706 27.173027907875486 22.671008198456004 40-41 23.373988298685457 26.701783262852842 27.137520064367003 22.786708374094694 42-43 23.25366721975712 26.671938759371145 27.009199322488158 23.065194698383575 44-45 23.13650936795728 26.772662295442135 27.094652127614605 22.99617620898598 46-47 23.28515240932898 26.70686744240418 26.86966246704774 23.138317681219103 48-49 23.15981486711188 26.922404426900748 26.857900097949955 23.059880608037417 50-51 23.24426400913756 26.90857497133011 26.87081813570376 22.976342883828572 52-53 23.50841910396741 26.795029798549958 26.72248155813952 22.974069539343116 54-55 23.018223820851762 26.630897825891296 27.09508379242494 23.255794560831998 56-57 23.095431035920573 26.80035634733628 26.80610759550091 23.298105021242236 58-59 23.167645224287018 26.749174741911837 26.971409936800427 23.111770097000715 60-61 23.27814102005563 26.79218295333082 27.014039201409528 22.915636825204018 62-63 23.175369366607413 26.760539886531916 26.968011570558346 23.09607917630233 64-65 22.942910948235763 26.632746075061174 26.97616114361619 23.448181833086874 66-67 23.003189741739938 26.734488589963863 26.811089566187647 23.451232102108555 68-69 22.99769011535617 26.703888665343417 26.90565483115638 23.39276638814403 70-71 22.990236365618635 26.57120411061636 26.82529851705867 23.61326100670633 72-73 22.985666261700306 26.745698382895693 26.686477805082326 23.58215755032167 74-75 22.86807391505429 26.86084784644518 26.67415619168582 23.596922046814708 76-77 22.902046650875967 26.48487474240667 26.80613675297935 23.80694185373801 78-79 22.798503253846032 26.5732293760028 26.823252971278222 23.805014398872952 80-81 22.942774901570413 26.729922450288925 26.578390516235668 23.748912131904994 82-83 22.958480225432996 26.617164819982044 26.731802491888217 23.69255246269674 84-85 22.837745366078437 26.672162471800682 26.65723405485431 23.83285810726657 86-87 22.725153630585567 26.854200444675435 26.535600798182617 23.885045126556378 88-89 22.59159017695028 26.59299360156003 26.69660716046379 24.118809061025907 90-91 22.736351473675647 26.468699645016297 26.7257786939392 24.069170187368858 92-93 22.770531359965155 26.334729360032476 26.756587012492318 24.138152267510055 94-95 22.70280917777848 26.272469252601706 26.753212768833052 24.271508800786766 96-97 22.493814265781243 26.09589009580843 26.936845120258035 24.473450518152298 98-99 22.28299704142097 26.144792380444105 26.84721407902905 24.72499649910587 100-101 22.301422235947797 26.210766735304126 26.836497643696553 24.651313385051527 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 58.0 1 47.0 2 49.0 3 68.0 4 70.0 5 76.0 6 119.5 7 267.5 8 416.0 9 446.5 10 385.5 11 303.5 12 310.0 13 348.5 14 391.5 15 465.0 16 548.0 17 632.0 18 689.5 19 768.0 20 908.5 21 1086.0 22 1295.0 23 1594.5 24 2044.5 25 2719.5 26 3820.0 27 5339.0 28 7352.5 29 10027.5 30 13180.0 31 16836.0 32 21631.5 33 25787.5 34 29163.5 35 33161.5 36 38418.5 37 43343.0 38 47248.5 39 51167.0 40 54275.0 41 57095.0 42 59813.5 43 62908.0 44 65856.0 45 68874.0 46 71161.0 47 72456.5 48 73688.0 49 74078.5 50 73640.5 51 72569.5 52 70500.0 53 67785.5 54 65159.5 55 60712.0 56 54620.0 57 48132.0 58 42040.5 59 35855.0 60 29275.0 61 24102.5 62 19611.5 63 14596.0 64 10100.0 65 7262.5 66 5369.0 67 3955.0 68 2943.0 69 2211.5 70 1678.0 71 1213.5 72 832.0 73 603.5 74 451.0 75 333.5 76 223.0 77 130.0 78 77.5 79 45.0 80 27.0 81 16.0 82 12.0 83 7.0 84 4.0 85 3.0 86 3.0 87 2.5 88 1.0 89 0.5 90 0.0 91 0.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16194453054776473 2 5.750871113201873E-4 3 0.007821184713954545 4 0.004198135912637367 5 0.029962038499781754 6 0.0011501742226403746 7 1.1501742226403744E-4 8 0.001897787467356618 9 0.0 10-11 0.10834641177272328 12-13 1.4377177783004682E-4 14-15 0.005607099335371826 16-17 1.4377177783004682E-4 18-19 2.5878920009408425E-4 20-21 7.476132447162434E-4 22-23 2.300348445280749E-4 24-25 0.001754015689526571 26-27 0.0027604181343368985 28-29 0.00678602791357821 30-31 0.01673503493941745 32-33 0.016850052361681488 34-35 0.012450635960082054 36-37 5.750871113201873E-4 38-39 1.7252613339605616E-4 40-41 0.005060766579617648 42-43 0.008741324092066847 44-45 0.008482534891972761 46-47 0.010207796225933322 48-49 0.012881951293572193 50-51 0.00626844951339004 52-53 0.0013802090671684493 54-55 9.48893733678309E-4 56-57 0.006555993069050134 58-59 0.009690217825745156 60-61 0.007389869380464406 62-63 0.018201507073283926 64-65 0.03401640263458908 66-67 0.036719312057793954 68-69 0.026942831165350775 70-71 0.042182639615335735 72-73 0.02593642872054044 74-75 0.025246324186956222 76-77 0.24737372093437854 78-79 0.07079322340351506 80-81 0.05460452121985178 82-83 0.01989801405167848 84-85 0.0328662284119487 86-87 0.08166236980746658 88-89 0.26097453111710095 90-91 0.3190583293604399 92-93 0.055122099620039944 94-95 0.00586588853546591 96-97 0.010869146403951539 98-99 0.0014664721338664775 100-101 0.0011789285782063837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1738867.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.48282010721786 #Duplication Level Percentage of deduplicated Percentage of total 1 82.30265483027681 55.54015250258014 2 10.345649592948348 13.963072207464903 3 3.127712858721502 6.332006525764057 4 1.3591836808318496 3.6688619130496765 5 0.7708411820369074 2.60092684093159 6 0.47984579608550576 1.9428808521865755 7 0.3303708764206297 1.560605089551008 8 0.24736639817694162 1.3354385718995978 9 0.17486476951922392 1.062033100610031 >10 0.824842523427432 9.337769170402423 >50 0.02573635575215288 1.1366089938562955 >100 0.010333537668158947 1.1790487816745447 >500 4.268558104126327E-4 0.1822807855027595 >1k 1.7074232416505291E-4 0.15831466452641677 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.750871113201872E-5 0.0 3 0.0 0.0 0.0 5.750871113201872E-5 0.0 4 0.0 0.0 0.0 5.750871113201872E-5 0.0 5 0.0 0.0 0.0 5.750871113201872E-5 0.0 6 0.0 0.0 0.0 5.750871113201872E-5 0.0 7 0.0 0.0 0.0 5.750871113201872E-5 0.0 8 0.0 0.0 0.0 5.750871113201872E-5 0.0 9 0.0 0.0 0.0 5.750871113201872E-5 0.0 10-11 0.0 0.0 0.0 1.1501742226403744E-4 0.0 12-13 0.0 0.0 0.0 1.1501742226403744E-4 5.750871113201872E-5 14-15 0.0 0.0 0.0 1.1501742226403744E-4 5.750871113201872E-5 16-17 0.0 0.0 0.0 1.1501742226403744E-4 5.750871113201872E-5 18-19 0.0 0.0 0.0 1.1501742226403744E-4 5.750871113201872E-5 20-21 0.0 0.0 0.0 1.437717778300468E-4 5.750871113201872E-5 22-23 0.0 0.0 0.0 2.5878920009408425E-4 5.750871113201872E-5 24-25 0.0 0.0 0.0 6.901045335842247E-4 5.750871113201872E-5 26-27 0.0 0.0 0.0 9.201393781122995E-4 5.750871113201872E-5 28-29 0.0 0.0 0.0 0.0027316637787708894 5.750871113201872E-5 30-31 0.0 0.0 0.0 0.011472987870837734 5.750871113201872E-5 32-33 0.0 0.0 0.0 0.029300688321763538 1.1501742226403744E-4 34-35 0.0 0.0 0.0 0.052534207619099105 1.1501742226403744E-4 36-37 0.0 0.0 0.0 0.09106504407755164 1.1501742226403744E-4 38-39 0.0 0.0 0.0 0.1480849311649482 1.1501742226403744E-4 40-41 0.0 0.0 0.0 0.21329980958865744 1.1501742226403744E-4 42-43 0.0 0.0 0.0 0.282569052147174 1.1501742226403744E-4 44-45 0.0 0.0 0.0 0.3698385212900124 1.1501742226403744E-4 46-47 0.0 0.0 0.0 0.4723765532384018 1.1501742226403744E-4 48-49 0.0 0.0 0.0 0.5814705782558414 2.875435556600936E-4 50-51 0.0 0.0 0.0 0.6898457443841306 2.875435556600936E-4 52-53 0.0 0.0 0.0 0.802016485447133 2.875435556600936E-4 54-55 0.0 0.0 0.0 0.9373057283852071 2.875435556600936E-4 56-57 0.0 0.0 0.0 1.0921191787526014 2.875435556600936E-4 58-59 2.875435556600936E-5 0.0 0.0 1.255645198856497 2.875435556600936E-4 60-61 5.750871113201872E-5 0.0 0.0 1.4285738932304772 2.875435556600936E-4 62-63 5.750871113201872E-5 0.0 0.0 1.6046368123611523 3.450522667921123E-4 64-65 5.750871113201872E-5 0.0 0.0 1.802495533010863 3.450522667921123E-4 66-67 5.750871113201872E-5 0.0 0.0 2.02703829562583 3.450522667921123E-4 68-69 5.750871113201872E-5 0.0 0.0 2.2731468249153037 3.450522667921123E-4 70-71 5.750871113201872E-5 0.0 0.0 2.5317634988759923 4.0256097792413105E-4 72-73 5.750871113201872E-5 0.0 0.0 2.799322777417709 4.888240446221592E-4 74-75 5.750871113201872E-5 0.0 0.0 3.085946193699691 5.175784001881685E-4 76-77 5.750871113201872E-5 0.0 0.0 3.402761683325982 5.175784001881685E-4 78-79 5.750871113201872E-5 0.0 0.0 3.7588268682998756 5.463327557541778E-4 80-81 5.750871113201872E-5 0.0 0.0 4.129758055101396 5.750871113201872E-4 82-83 5.750871113201872E-5 0.0 0.0 4.51998341448771 5.750871113201872E-4 84-85 5.750871113201872E-5 0.0 0.0 4.926685019613346 5.750871113201872E-4 86-87 5.750871113201872E-5 0.0 0.0 5.370249708574606 6.038414668861965E-4 88-89 5.750871113201872E-5 0.0 0.0 5.853092847239036 6.32595822452206E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1580 0.0 55.67129 1 GTATCAA 2810 0.0 38.409283 1 TCAACGC 3515 0.0 29.843233 4 ATCAACG 3685 0.0 28.595282 3 CAACGCA 3715 0.0 28.290312 5 TATCAAC 3880 0.0 27.76982 2 AACGCAG 3905 0.0 27.032495 6 GTACATG 4550 0.0 26.960316 1 TACATGG 4845 0.0 25.079859 2 ACATGGG 4960 0.0 24.21128 3 CATGGGG 4080 0.0 23.267447 4 TAGATCG 105 0.0021817097 22.64566 5 ACGCAGA 4560 0.0 22.4196 7 CGCAGAG 4720 0.0 21.357384 8 TACACTG 1975 0.0 19.744722 5 ATGGGGG 2650 0.0 19.381268 5 AGAGTAC 4575 0.0 19.176067 10-11 TATACTG 810 0.0 18.200401 5 GCAGAGT 5295 0.0 17.960491 9 GTACACT 1475 0.0 17.699034 4 >>END_MODULE