FastQCFastQC Report
Mon 15 Aug 2016
SRR2921391_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921391_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523239
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.050.7482381
GTATCAA21300.040.764791
TCAACGC26450.031.5729374
CAACGCA26650.031.1569255
ATCAACG27300.030.7626953
TATCAAC28000.029.9955982
GTACATG36750.029.5659521
AACGCAG29350.028.1234956
TACATGG38300.027.6279322
ACATGGG39200.025.7814463
CATGGGG34500.023.3807164
ACGCAGA34300.023.0997667
CGCAGAG34200.022.7526238
GTATAGC3600.022.4057851
GTATAGA5050.018.7911071
ATGGGGG21250.018.309175
GCAGAGT40550.018.1365479
TATTCCG1851.5346735E-417.9531255
TAAGACG1600.00119985217.7934154
AGAGTAC36250.017.6701210-11
Produced by FastQC (version 0.11.5)