##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921391_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1523239
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.18461843479585	31.0	31.0	34.0	26.0	34.0
2	30.279933089948457	31.0	31.0	34.0	26.0	34.0
3	30.318170031098205	33.0	31.0	34.0	26.0	34.0
4	33.76234655231385	37.0	35.0	37.0	28.0	37.0
5	33.753573798990175	37.0	35.0	37.0	28.0	37.0
6	33.7894059960387	37.0	35.0	37.0	29.0	37.0
7	33.69827847107381	37.0	35.0	37.0	28.0	37.0
8	33.661758266430944	37.0	35.0	37.0	28.0	37.0
9	35.20534072460067	39.0	35.0	39.0	28.0	39.0
10-11	35.09690501621873	39.0	35.0	39.0	28.0	39.0
12-13	34.98540839618734	39.0	35.0	39.0	27.0	39.0
14-15	36.10543224011465	40.0	36.0	41.0	27.0	41.0
16-17	36.06091099295645	40.0	36.0	41.0	27.0	41.0
18-19	36.01449805316172	40.0	36.0	41.0	27.0	41.0
20-21	35.96030005796858	40.0	36.0	41.0	27.0	41.0
22-23	35.839713268896084	40.0	36.0	41.0	27.0	41.0
24-25	35.75171854187032	40.0	36.0	41.0	27.0	41.0
26-27	35.60447900821867	40.0	36.0	41.0	26.0	41.0
28-29	35.46318470049677	40.0	36.0	41.0	25.0	41.0
30-31	35.31658163951947	40.0	35.5	41.0	25.0	41.0
32-33	35.139920918516395	39.0	35.0	41.0	24.0	41.0
34-35	34.9744921840893	39.0	35.0	41.0	24.0	41.0
36-37	34.8100124143355	39.0	35.0	41.0	23.5	41.0
38-39	35.0263727491221	39.0	35.0	41.0	24.0	41.0
40-41	35.14188023021995	40.0	35.0	41.0	24.0	41.0
42-43	34.98510246914634	40.0	35.0	41.0	22.5	41.0
44-45	34.86539899516754	40.0	35.0	41.0	21.5	41.0
46-47	34.706483027285934	40.0	35.0	41.0	19.5	41.0
48-49	34.56255453018207	39.5	35.0	41.0	18.0	41.0
50-51	32.87425643644891	37.0	32.5	39.5	14.0	40.0
52-53	33.03425496589833	37.5	33.0	39.5	13.0	40.0
54-55	33.9029374247902	39.0	34.0	40.5	13.0	41.0
56-57	33.92621052901087	39.0	34.0	41.0	12.5	41.0
58-59	33.760780153344285	38.5	34.0	41.0	9.0	41.0
60-61	33.53680020009992	38.0	34.0	40.5	8.0	41.0
62-63	33.27071753021029	37.5	33.5	40.0	7.0	41.0
64-65	32.98739495246642	37.0	33.0	40.0	2.0	41.0
66-67	32.65809173740956	36.5	33.0	40.0	2.0	41.0
68-69	32.29895111666652	36.0	32.5	39.0	2.0	41.0
70-71	31.92845279040256	35.5	32.0	39.0	2.0	41.0
72-73	31.570776811780686	35.0	32.0	38.5	2.0	40.0
74-75	31.175469837628896	35.0	32.0	37.0	2.0	39.5
76-77	30.790914623378207	35.0	31.0	37.0	2.0	39.0
78-79	30.439318124076394	35.0	31.0	36.0	2.0	39.0
80-81	30.082522178069233	35.0	31.0	36.0	2.0	37.5
82-83	29.778234078828078	35.0	31.0	35.5	2.0	37.0
84-85	29.532964295163136	35.0	31.0	35.0	2.0	37.0
86-87	29.30284610622496	35.0	30.5	35.0	2.0	36.0
88-89	29.104580108571277	35.0	30.0	35.0	2.0	36.0
90-91	28.857064781035675	34.0	30.0	35.0	2.0	36.0
92-93	28.673893591222388	34.0	30.0	35.0	2.0	35.0
94-95	28.485676902967953	34.0	30.0	35.0	2.0	35.0
96-97	28.246856862252084	34.0	29.5	35.0	2.0	35.0
98-99	28.025934538178184	34.0	29.0	35.0	2.0	35.0
100-101	27.005960653580956	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59582.0
3	9809.0
4	6456.0
5	4790.0
6	4437.0
7	4446.0
8	4767.0
9	5032.0
10	5567.0
11	5820.0
12	5994.0
13	6130.0
14	6619.0
15	7034.0
16	7230.0
17	7943.0
18	7295.0
19	6406.0
20	6520.0
21	6776.0
22	7331.0
23	8332.0
24	9610.0
25	11251.0
26	13521.0
27	15900.0
28	18599.0
29	22503.0
30	26282.0
31	32361.0
32	40005.0
33	51642.0
34	71455.0
35	103688.0
36	171466.0
37	313607.0
38	355598.0
39	71424.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.50969713210799	20.222566390281322	14.471347352270458	24.796389125340227
2	15.230005859185129	19.88411687891981	40.03170003074102	24.854177231154047
3	18.42920898889688	24.112784843797723	29.8837144386537	27.5742917286517
4	12.878161885932357	16.085337256885374	37.2754740776259	33.76102677955637
5	14.0235015635088	37.21157515383863	34.8505159480525	13.91440733460008
6	33.20277874444349	35.976729985095105	17.098076809434072	13.722414461027322
7	28.38781901866674	31.77678706263173	21.578760759801185	18.25663315890035
8	26.314033871238863	35.09104733988906	19.999225184905026	18.595693603967053
9	26.408321229615193	15.433094235653483	19.53605769862136	38.62252683610997
10-11	25.186458073075606	25.454165803472844	28.479913219883123	20.87946290356842
12-13	25.68799838985213	22.876412285848527	28.274084991773602	23.16150433252574
14-15	22.91269730244142	24.630910699402797	25.328402291082554	27.127989707073237
16-17	23.110562567760752	27.663756935319384	26.10648263978498	23.119197857134882
18-19	23.08898758194462	27.11977482486579	27.401473566775568	22.38976402641402
20-21	23.619936362970748	26.26329287315835	27.364086743620785	22.752684020250115
22-23	23.353537250925168	26.071756144833746	27.170432419882438	23.40427418435864
24-25	23.26822668671969	26.285637351162134	27.1012655733829	23.34487038873528
26-27	23.184794479077535	26.491260443830367	27.020125572223076	23.30381950486903
28-29	23.221332713478816	26.609586207711672	26.966527856025657	23.202553222783855
30-31	23.441696962215975	26.39987104831027	26.91902634908483	23.23940564038893
32-33	23.284182494851784	26.382402211804145	26.855220650245993	23.478194643098075
34-35	23.525240717232467	26.469523266460047	26.847205822056203	23.158030194251282
36-37	23.49736282695497	26.39726842180588	26.82816424657732	23.277204504661828
38-39	23.38270698757244	26.439076464714173	26.867662806019382	23.310553741694008
40-41	23.444813989550905	26.349040240442122	26.861023612225367	23.34512215778161
42-43	23.332107457753345	26.40164289257514	26.740030605222888	23.526219044448627
44-45	23.37240617469944	26.4254549317253	26.754421880791163	23.447717012784096
46-47	23.489898672637537	26.447596003474384	26.53064043536989	23.531864888518193
48-49	23.315275767057393	26.601879995237855	26.52640261312892	23.55644162457583
50-51	23.44722800036176	26.54742779152655	26.584656367418418	23.420687840693265
52-53	23.54779296894271	26.453062150757606	26.604122334739895	23.39502254555979
54-55	23.297831124640776	26.39252217188796	26.802742387755945	23.506904315715317
56-57	23.336167525421388	26.55750915630432	26.595894094409843	23.510429223864445
58-59	23.373671744901344	26.487446720965043	26.70028328872788	23.438598245405725
60-61	23.402664572059358	26.48698041042199	26.779429430022088	23.330925587496562
62-63	23.393915432844718	26.47066949710586	26.705614808100037	23.429800261949385
64-65	23.219129015005848	26.3380173286557	26.74459145566102	23.698262200677437
66-67	23.248915742138205	26.48443921002684	26.63733872620133	23.62930632163362
68-69	23.363957922570822	26.3846924193864	26.671117564547615	23.580232093495166
70-71	23.22750912156741	26.30852074087909	26.67394243745117	23.79002770010233
72-73	23.268939735247866	26.36810635861569	26.600506037393096	23.762447868743344
74-75	23.24416101529373	26.487000033543207	26.618311829505792	23.650527121657273
76-77	23.21735927732159	26.290682723263757	26.64028202084244	23.851675978572203
78-79	23.229551211646648	26.234433586316854	26.68921841546334	23.846796786573158
80-81	23.291059540124465	26.415934123412022	26.477285511927168	23.815720824536342
82-83	23.33850885705357	26.376815408148158	26.5997467063603	23.684929028437967
84-85	23.262769818604532	26.441516030075345	26.602530576316948	23.693183575003175
86-87	23.24818520827542	26.472979219339354	26.51255673023304	23.76627884215219
88-89	23.138185352090808	26.277666909458418	26.669193279998105	23.914954458452673
90-91	23.146219338877778	26.31030240025266	26.663793557216547	23.879684703653016
92-93	23.19353193729335	26.19845042851738	26.79215673372703	23.81586090046224
94-95	23.11568817322116	26.149236660938207	26.801444979341692	23.933630186498938
96-97	23.1269081831382	26.05220496227148	26.81046047118119	24.010426383409126
98-99	23.023534010020015	25.965219462572332	26.77686184247755	24.234384684930102
100-101	23.15727546213505	25.86505238698622	26.967279973177234	24.0103921777015
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	38.5
2	52.0
3	77.0
4	79.0
5	80.5
6	118.0
7	260.5
8	386.0
9	386.5
10	352.5
11	330.5
12	321.5
13	375.0
14	478.0
15	566.5
16	628.0
17	698.5
18	785.5
19	866.5
20	969.5
21	1097.5
22	1272.0
23	1530.5
24	1918.0
25	2468.5
26	3088.5
27	4028.5
28	5486.0
29	7323.5
30	9758.5
31	12738.0
32	16349.5
33	19643.0
34	22620.5
35	26070.0
36	30515.0
37	35081.0
38	38930.0
39	42680.5
40	46156.5
41	49171.0
42	52068.0
43	55622.0
44	58936.0
45	61782.0
46	63548.0
47	64607.0
48	65861.0
49	65831.5
50	64326.5
51	62875.0
52	61612.0
53	59674.0
54	57539.5
55	54231.0
56	49315.0
57	43898.0
58	38950.5
59	34006.5
60	28220.5
61	22886.5
62	18579.0
63	14017.5
64	9878.5
65	7248.5
66	5395.5
67	4128.5
68	3135.0
69	2378.0
70	1843.5
71	1348.0
72	932.0
73	663.5
74	511.5
75	379.0
76	266.0
77	200.5
78	148.5
79	104.5
80	71.0
81	60.0
82	55.5
83	46.5
84	44.0
85	37.0
86	29.0
87	23.5
88	18.5
89	20.0
90	17.0
91	11.5
92	8.5
93	10.0
94	10.0
95	8.5
96	7.5
97	4.5
98	5.0
99	3.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.047727244378590626
2	0.05534259561368899
3	0.04556080825136436
4	0.05311050990684981
5	0.046086004888267695
6	0.01634674532361632
7	0.01372076213909964
8	0.0193666259858105
9	0.019104027667358834
10-11	0.0203185448901978
12-13	0.02609570789613449
14-15	0.04201573095226684
16-17	0.02744152427819928
18-19	0.022025433960133636
20-21	0.02517661378155365
22-23	0.04460888934697707
24-25	0.03998059398426643
26-27	0.029542310825812625
28-29	0.019760523463488004
30-31	0.012342120967228387
32-33	0.009092466776388997
34-35	0.00882986845793733
36-37	0.001116042853419588
38-39	0.006039761324388359
40-41	0.002954231082581263
42-43	0.21250768920701218
44-45	0.18864406701771685
46-47	0.19488077708094395
48-49	0.19143417415126582
50-51	0.19048225524687853
52-53	0.19589834556494418
54-55	0.19419145649500832
56-57	0.20426866696559107
58-59	0.20078923924610648
60-61	0.2064679278826238
62-63	0.20338239764081673
64-65	0.20338239764081673
66-67	0.19428993086442772
68-69	0.19343648632945978
70-71	0.1750874288276495
72-73	0.1836875237569416
74-75	0.18460661787152247
76-77	0.18276842964236079
78-79	0.20873283837926945
80-81	0.21703751020030346
82-83	0.2142802278565609
84-85	0.20942215896520505
86-87	0.2256047803397891
88-89	0.22439026311695015
90-91	0.22314292110430473
92-93	0.2274101437791443
94-95	0.2384720979439208
96-97	0.2384720979439208
98-99	0.2285261866325639
100-101	0.23860339710314668
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1523239.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.15566927497747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.42495815603411	60.073144011045756
2	9.208719296786445	13.105051694564787
3	2.7103597332672695	5.785723823897459
4	1.220827964292988	3.4747532347550347
5	0.6671773079535221	2.3736723936255313
6	0.42212971877999456	1.8022153596389126
7	0.2898659347560466	1.4437923211308417
8	0.20532027540645756	1.1687761289815348
9	0.15096311974803803	0.9667693639359194
>10	0.6759876455570435	8.00345296607293
>50	0.01711917362743405	0.8041197242410071
>100	0.006294027501017045	0.8171679106547088
>500	1.8509752647280243E-4	0.10688054193191218
>1k	9.254876323640121E-5	0.07448052552371699
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.9694873883875085E-4	0.0
12-13	0.0	0.0	0.0	2.6259831845166777E-4	3.282478980645847E-5
14-15	3.282478980645847E-5	3.282478980645847E-5	0.0	2.6259831845166777E-4	1.3129915922583388E-4
16-17	6.564957961291694E-5	6.564957961291694E-5	0.0	3.938974776775017E-4	2.6259831845166777E-4
18-19	6.564957961291694E-5	6.564957961291694E-5	0.0	4.5954705729041865E-4	2.6259831845166777E-4
20-21	6.564957961291694E-5	6.564957961291694E-5	0.0	4.5954705729041865E-4	2.6259831845166777E-4
22-23	6.564957961291694E-5	6.564957961291694E-5	0.0	5.908462165162525E-4	2.9542310825812627E-4
24-25	9.847436941937541E-5	6.564957961291694E-5	0.0	6.893205859356279E-4	3.2824789806458476E-4
26-27	1.3129915922583388E-4	6.564957961291694E-5	0.0	9.190941145808373E-4	3.2824789806458476E-4
28-29	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.0016084147005164654	3.2824789806458476E-4
30-31	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.005120667209807522	3.938974776775017E-4
32-33	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.013195565502196308	3.938974776775017E-4
34-35	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.023174301603359683	4.5954705729041865E-4
36-37	1.3129915922583388E-4	9.847436941937541E-5	0.0	0.03942257255755663	4.5954705729041865E-4
38-39	1.3129915922583388E-4	1.3129915922583388E-4	0.0	0.06978550312853071	5.251966369033355E-4
40-41	1.3129915922583388E-4	1.3129915922583388E-4	0.0	0.10471107948260253	5.251966369033355E-4
42-43	1.6412394903229238E-4	1.3129915922583388E-4	0.0	0.14308325876635247	5.251966369033355E-4
44-45	1.9694873883875085E-4	1.3129915922583388E-4	0.0	0.1889066653361685	5.251966369033355E-4
46-47	1.9694873883875085E-4	1.3129915922583388E-4	0.0	0.24063853407114705	5.58021426709794E-4
48-49	1.9694873883875085E-4	1.3129915922583388E-4	0.0	0.29762236917515894	7.221453757420864E-4
50-51	1.9694873883875085E-4	1.3129915922583388E-4	0.0	0.36081008955259153	7.221453757420864E-4
52-53	1.9694873883875085E-4	1.3129915922583388E-4	0.0	0.4238993355606048	7.877949553550034E-4
54-55	1.9694873883875085E-4	1.3129915922583388E-4	0.0	0.49266727020513523	8.534445349679203E-4
56-57	1.9694873883875085E-4	1.3129915922583388E-4	0.0	0.5777163005936692	8.534445349679203E-4
58-59	1.9694873883875085E-4	1.3129915922583388E-4	0.0	0.6712669515420757	8.534445349679203E-4
60-61	1.9694873883875085E-4	1.3129915922583388E-4	0.0	0.7689207012162897	8.534445349679203E-4
62-63	2.6259831845166777E-4	1.3129915922583388E-4	0.0	0.8742226269154085	9.190941145808373E-4
64-65	2.6259831845166777E-4	1.3129915922583388E-4	0.0	0.9894704639258842	9.190941145808373E-4
66-67	2.6259831845166777E-4	1.3129915922583388E-4	0.0	1.1207696231517181	9.190941145808373E-4
68-69	3.2824789806458476E-4	1.9694873883875085E-4	0.0	1.2622444672175543	9.190941145808373E-4
70-71	3.2824789806458476E-4	1.9694873883875085E-4	0.0	1.4197049839191354	9.190941145808373E-4
72-73	3.2824789806458476E-4	1.9694873883875085E-4	0.0	1.5840915312698796	0.001050393273806671
74-75	3.2824789806458476E-4	1.9694873883875085E-4	0.0	1.7622316655495296	0.0011488676432260467
76-77	3.2824789806458476E-4	1.9694873883875085E-4	0.0	1.9600994985028612	0.001181692433032505
78-79	3.938974776775017E-4	1.9694873883875085E-4	0.0	2.1887898090844575	0.001181692433032505
80-81	3.938974776775017E-4	1.9694873883875085E-4	0.0	2.4296252918944434	0.001181692433032505
82-83	3.938974776775017E-4	2.6259831845166777E-4	0.0	2.6846082591110125	0.001181692433032505
84-85	4.2672226748396015E-4	2.6259831845166777E-4	0.0	2.9530822149380365	0.001181692433032505
86-87	4.5954705729041865E-4	2.6259831845166777E-4	0.0	3.247028207654872	0.0012145172228389635
88-89	4.5954705729041865E-4	2.6259831845166777E-4	0.0	3.576786046050554	0.001247342012645422
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1150	0.0	50.748238	1
GTATCAA	2130	0.0	40.76479	1
TCAACGC	2645	0.0	31.572937	4
CAACGCA	2665	0.0	31.156925	5
ATCAACG	2730	0.0	30.762695	3
TATCAAC	2800	0.0	29.995598	2
GTACATG	3675	0.0	29.565952	1
AACGCAG	2935	0.0	28.123495	6
TACATGG	3830	0.0	27.627932	2
ACATGGG	3920	0.0	25.781446	3
CATGGGG	3450	0.0	23.380716	4
ACGCAGA	3430	0.0	23.099766	7
CGCAGAG	3420	0.0	22.752623	8
GTATAGC	360	0.0	22.405785	1
GTATAGA	505	0.0	18.791107	1
ATGGGGG	2125	0.0	18.30917	5
GCAGAGT	4055	0.0	18.136547	9
TATTCCG	185	1.5346735E-4	17.953125	5
TAAGACG	160	0.001199852	17.793415	4
AGAGTAC	3625	0.0	17.67012	10-11
>>END_MODULE