FastQCFastQC Report
Mon 15 Aug 2016
SRR2921391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523239
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13900.050.272831
GTATCAA23700.040.115521
TCAACGC29850.030.9981674
CAACGCA30200.030.2231255
ATCAACG31550.029.32793
TATCAAC32000.028.9164282
AACGCAG32900.027.7409846
GTACATG37500.025.860071
TACATGG38850.024.4286212
ACGCAGA38300.023.5814957
CGCAGAG38500.022.8416528
ACATGGG41400.021.7770463
CATGGGG35600.020.3932844
GTATATA6000.019.8070411
AGAGTAC38350.019.27443110-11
GCAGAGT46700.018.220189
ACCGTGC3451.0732037E-917.9118638
GAGTACT24550.017.68135512-13
CAGAGTA42550.017.5394769
TCTATAC4600.017.5362553
Produced by FastQC (version 0.11.5)