##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921391_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1523239
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.435317766942678	33.0	31.0	34.0	29.0	34.0
2	31.569070907454446	34.0	31.0	34.0	29.0	34.0
3	31.643725639902865	34.0	31.0	34.0	28.0	34.0
4	35.13670277612378	37.0	35.0	37.0	33.0	37.0
5	34.88553142349953	37.0	35.0	37.0	32.0	37.0
6	34.85004585623136	37.0	35.0	37.0	32.0	37.0
7	34.71468233153169	37.0	35.0	37.0	32.0	37.0
8	34.68089052341753	37.0	35.0	37.0	32.0	37.0
9	36.3090473655152	39.0	37.0	39.0	32.0	39.0
10-11	36.15757376222641	39.0	37.0	39.0	32.0	39.0
12-13	36.09475236650322	39.0	37.0	39.0	32.0	39.0
14-15	37.3481390641915	40.0	38.0	41.0	32.0	41.0
16-17	37.333278297102424	40.0	38.0	41.0	32.0	41.0
18-19	37.305685122295316	40.0	38.0	41.0	31.5	41.0
20-21	37.27182569511416	40.0	38.0	41.0	31.0	41.0
22-23	37.160658635972425	40.0	38.0	41.0	31.0	41.0
24-25	37.104145508354236	40.0	38.0	41.0	31.0	41.0
26-27	36.973346270677155	40.0	37.0	41.0	30.5	41.0
28-29	36.82486070800446	40.0	37.0	41.0	30.0	41.0
30-31	36.68573644713666	40.0	37.0	41.0	30.0	41.0
32-33	36.63198913630757	40.0	37.0	41.0	30.0	41.0
34-35	36.753640433313485	40.0	37.0	41.0	30.0	41.0
36-37	36.734961814922016	40.0	37.0	41.0	30.0	41.0
38-39	36.65067136542591	40.0	37.0	41.0	30.0	41.0
40-41	36.52860122410206	40.0	37.0	41.0	30.0	41.0
42-43	36.42119719886374	40.0	37.0	41.0	30.0	41.0
44-45	36.28049800458103	40.0	36.5	41.0	29.0	41.0
46-47	36.11732039423886	40.0	36.0	41.0	28.5	41.0
48-49	35.95618448582265	40.0	36.0	41.0	28.0	41.0
50-51	35.81615688673938	40.0	35.5	41.0	28.0	41.0
52-53	35.65674034081323	39.5	35.0	41.0	27.0	41.0
54-55	35.458456946020945	39.0	35.0	41.0	27.0	41.0
56-57	35.20827723029676	39.0	35.0	41.0	26.0	41.0
58-59	34.996318043327406	39.0	35.0	41.0	26.0	41.0
60-61	34.734588597061915	38.0	35.0	40.0	26.0	41.0
62-63	34.4577702514182	38.0	34.0	40.0	26.0	41.0
64-65	34.150557791653185	37.0	34.0	40.0	25.0	41.0
66-67	33.82243036056718	37.0	34.0	39.5	24.0	41.0
68-69	33.47186160543421	36.0	34.0	39.0	23.0	41.0
70-71	33.09378633293922	36.0	33.5	39.0	22.5	40.5
72-73	32.69359765604741	35.0	33.0	38.5	22.0	40.0
74-75	32.27708553943275	35.0	33.0	37.0	20.5	39.5
76-77	29.514633947791516	33.0	29.0	34.5	14.0	36.5
78-79	31.160697040976498	34.5	31.5	36.0	18.5	38.0
80-81	31.345202886743316	35.0	32.0	36.0	19.0	37.0
82-83	31.17231307759321	35.0	32.0	35.5	18.5	37.0
84-85	30.929419808710257	35.0	32.0	35.0	17.5	37.0
86-87	30.670675448829762	35.0	32.0	35.0	12.5	36.0
88-89	30.385916130036062	35.0	32.0	35.0	7.0	36.0
90-91	30.19508297778615	35.0	32.0	35.0	4.5	35.5
92-93	30.053188961154486	34.0	32.0	35.0	2.0	35.0
94-95	29.95911114408179	34.0	32.0	35.0	2.0	35.0
96-97	29.771268330183247	34.0	31.0	35.0	2.0	35.0
98-99	29.511477516003726	34.0	31.0	35.0	2.0	35.0
100-101	28.442073765180645	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27429.0
3	9076.0
4	4979.0
5	3427.0
6	3137.0
7	3111.0
8	3146.0
9	3466.0
10	3910.0
11	4190.0
12	4680.0
13	5062.0
14	5530.0
15	6320.0
16	6971.0
17	7815.0
18	6974.0
19	5923.0
20	5484.0
21	5393.0
22	5630.0
23	6532.0
24	7429.0
25	8787.0
26	10671.0
27	13052.0
28	15557.0
29	18866.0
30	22918.0
31	28164.0
32	36112.0
33	47822.0
34	68411.0
35	102657.0
36	179934.0
37	357454.0
38	409297.0
39	57921.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.583931897480674	21.3807223006428	14.657912782437354	24.37743301943917
2	15.147876551801106	21.7386737393565	38.132324074499586	24.98112563434281
3	17.276610384034097	25.665760209461208	29.663801106394267	27.393828300110428
4	12.76548111908129	17.67698850692434	36.31624143731067	33.2412889366837
5	13.820467679966693	37.74771337709693	34.76966981618369	13.662149126752684
6	32.84907442846442	36.87298166188623	16.895250262437294	13.38269364721206
7	28.398498200216775	32.1809643791946	21.56299832134025	17.857539099248378
8	26.269215240233585	35.892804973723166	19.722296079647847	18.115683706395398
9	26.285566480375046	15.845707732010538	19.505671795430658	38.36305399218376
10-11	25.22836151434899	25.962634141853574	28.278022750721227	20.530981593076213
12-13	25.824568820776566	23.236754920693485	28.316041741411617	22.622634517118332
14-15	22.95835631187794	24.971473778197957	25.2656976286142	26.80447228130991
16-17	23.09942326692861	28.083979162768713	26.061277478524325	22.75532009177835
18-19	23.155624633796876	27.33233436130072	27.55780880463094	21.954232200271463
20-21	23.662190972786405	26.479202876786296	27.445309384185883	22.41329676624142
22-23	23.444586576017578	26.20705977042961	27.296319383103018	23.052034270449795
24-25	23.367963918540983	26.482517298880005	27.179855831724897	22.96966295085411
26-27	23.24932059218692	26.634099296777215	27.19522006773234	22.92136004330353
28-29	23.27393434378582	26.834853651586915	27.050886783013578	22.840325221613686
30-31	23.55652527332406	26.57692764783399	26.966715232805583	22.899831846036367
32-33	23.38965166981942	26.621707422608797	26.890090603535498	23.098550304036287
34-35	23.54452636599971	26.67888760164917	27.000310885353557	22.77627514699756
36-37	23.56236041139004	26.62372491015778	26.95443748990629	22.85947718854589
38-39	23.505575135574812	26.62469025656883	26.926186445768597	22.943548162087765
40-41	23.594508287989242	26.563451963276542	26.912165959368895	22.929873789365324
42-43	23.511190669949837	26.526248890846855	26.83354545254597	23.129014986657335
44-45	23.480365814545983	26.571386458663998	26.83778017709027	23.110467549699745
46-47	23.651656604406604	26.582618809461362	26.650145541938958	23.11557904419308
48-49	23.423008778671186	26.75372349139056	26.60478055291742	23.218487177020833
50-51	23.52199874602388	26.729551490163512	26.60139644356615	23.147053320246464
52-53	23.7324031966555	26.65207911939008	26.505744092929636	23.109773591024783
54-55	23.430275898810514	26.535208438168713	26.7438451991388	23.290670463881977
56-57	23.450737422069086	26.716187044691768	26.58074351473006	23.252332018509083
58-59	23.49342475224025	26.621685990761595	26.648440662242134	23.236448594756016
60-61	23.541470934465742	26.662090554497425	26.69971052971945	23.096727981317382
62-63	23.521273710868737	26.636311764478	26.678006254830162	23.164408269823102
64-65	23.337605250550737	26.47455845089315	26.702076624600924	23.485759673955194
66-67	23.284070912405006	26.68018655075818	26.571661898258714	23.4640806385781
68-69	23.34071315836045	26.60496419367559	26.593045716062463	23.461276931901494
70-71	23.35862756984211	26.445360705576533	26.59280460629195	23.603207118289415
72-73	23.379996644470484	26.564991714600065	26.46301251034649	23.59199913058296
74-75	23.281402004693092	26.680167433143794	26.424434286036984	23.613996276126134
76-77	23.294439402923	26.424631959794766	26.59424862941176	23.686680007870475
78-79	23.221809959388313	26.425219186163385	26.65701849007569	23.69595236437261
80-81	23.29219401765009	26.60781770703846	26.43280127769547	23.66718699761598
82-83	23.282292378000736	26.59442795608552	26.50811577454431	23.61516389136944
84-85	23.274484128182866	26.613942296957767	26.4623756043295	23.64919797052987
86-87	23.186204748745403	26.591423664357865	26.411529025586038	23.810842561310693
88-89	23.10006766665877	26.455030634302517	26.50703127674084	23.937870422297873
90-91	23.112841901999566	26.474909788781602	26.48274708390055	23.929501225318283
92-93	23.05648815953292	26.442204589320177	26.637052499245467	23.864254751901434
94-95	22.995584533162287	26.333856037595314	26.681585129944587	23.988974299297812
96-97	22.901707352063028	26.26034123405092	26.69219372860229	24.145757685283762
98-99	22.78524516549196	26.272669142130862	26.599083780632554	24.343001911744626
100-101	22.748512116269442	26.212193178860183	26.65635080410223	24.382943900768144
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	27.5
2	34.5
3	51.5
4	43.5
5	43.5
6	84.0
7	234.0
8	371.5
9	398.0
10	346.5
11	292.0
12	292.0
13	293.5
14	322.5
15	376.5
16	433.5
17	492.0
18	557.0
19	620.5
20	733.5
21	883.5
22	1070.5
23	1362.5
24	1731.5
25	2241.5
26	2935.0
27	3901.5
28	5277.0
29	7172.0
30	9718.0
31	12775.5
32	16745.5
33	20343.5
34	23086.0
35	26595.0
36	31135.5
37	35867.0
38	40204.0
39	43698.0
40	46764.5
41	50526.0
42	53837.5
43	56846.0
44	60091.5
45	63158.5
46	65231.0
47	66283.0
48	67132.5
49	66927.5
50	65237.0
51	63508.0
52	61865.0
53	59667.5
54	57294.0
55	53585.0
56	48386.0
57	42565.5
58	37381.5
59	32650.5
60	26898.0
61	21538.0
62	17251.5
63	12764.0
64	8852.0
65	6377.0
66	4756.0
67	3556.0
68	2620.0
69	2013.0
70	1494.5
71	1020.5
72	711.5
73	519.5
74	390.5
75	280.0
76	183.5
77	106.0
78	70.0
79	38.5
80	17.0
81	8.5
82	7.0
83	4.5
84	3.0
85	3.0
86	1.5
87	1.5
88	1.0
89	1.5
90	1.5
91	0.5
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14692375917370815
2	5.908462165162525E-4
3	0.006105410904001277
4	0.004004624356387934
5	0.023633848660650103
6	0.001181692433032505
7	0.0
8	0.002232085706839176
9	0.0
10-11	0.10037820722815002
12-13	2.2977352864520932E-4
14-15	0.005186316789420439
16-17	2.625983184516678E-4
18-19	4.2672226748396015E-4
20-21	7.549701655485449E-4
22-23	1.6412394903229236E-4
24-25	0.001247342012645422
26-27	0.0024618592354843857
28-29	0.006105410904001277
30-31	0.0150994033109709
32-33	0.0150994033109709
34-35	0.011127603744389423
36-37	8.534445349679203E-4
38-39	1.6412394903229236E-4
40-41	0.0046611201525171035
42-43	0.007713825604517742
44-45	0.007713825604517742
46-47	0.009092466776388997
48-49	0.011324552483228174
50-51	0.005514564687485023
52-53	0.0014442907514841728
54-55	0.0011488676432260467
56-57	0.0062367100632271105
58-59	0.00922376593561483
60-61	0.007352752916646698
62-63	0.01680629238090674
64-65	0.03305456333510368
66-67	0.03568054651962036
68-69	0.025833109577682818
70-71	0.04109663683768601
72-73	0.02491401546310198
74-75	0.023797972609682395
76-77	0.23870187147256605
78-79	0.06676562246633654
80-81	0.05320898427626919
82-83	0.01831623271200383
84-85	0.03131484947536139
86-87	0.08120852998117827
88-89	0.26437085710121655
90-91	0.31892565775955056
92-93	0.05051735151213959
94-95	0.004628295362710645
96-97	0.009814612152131084
98-99	0.0015099403310970898
100-101	0.0012145172228389637
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1523239.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.13340449448633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.7777916994425	58.7562175291182
2	9.583121596452289	13.44193886487672
3	2.7810600469919033	5.851356275974149
4	1.2573939078759444	3.52741262199866
5	0.7115767068071933	2.49526485036817
6	0.4621487938743729	1.944724097845823
7	0.3019692722915328	1.4824693182969428
8	0.22633084544637574	1.269868218661579
9	0.15983670646903042	1.008890314907309
>10	0.7108765331457223	8.214399273240659
>50	0.021419590584275887	1.0028367143072288
>100	0.006192835016990679	0.7965608352728282
>500	1.8764373455242494E-4	0.12403388534888518
>1k	9.382186727621247E-5	0.08402719978277261
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	6.564957961291694E-5
3	0.0	0.0	0.0	0.0	6.564957961291694E-5
4	0.0	0.0	0.0	0.0	6.564957961291694E-5
5	0.0	0.0	0.0	0.0	1.3129915922583388E-4
6	0.0	0.0	0.0	0.0	1.3129915922583388E-4
7	0.0	0.0	0.0	0.0	1.3129915922583388E-4
8	0.0	0.0	0.0	0.0	1.3129915922583388E-4
9	0.0	0.0	0.0	0.0	1.3129915922583388E-4
10-11	0.0	0.0	0.0	1.3129915922583388E-4	1.3129915922583388E-4
12-13	0.0	0.0	0.0	1.3129915922583388E-4	1.3129915922583388E-4
14-15	0.0	0.0	0.0	1.3129915922583388E-4	2.2977352864520932E-4
16-17	3.282478980645847E-5	0.0	0.0	1.9694873883875085E-4	2.6259831845166777E-4
18-19	6.564957961291694E-5	0.0	0.0	1.9694873883875085E-4	2.6259831845166777E-4
20-21	6.564957961291694E-5	0.0	0.0	2.6259831845166777E-4	2.9542310825812627E-4
22-23	6.564957961291694E-5	0.0	0.0	2.6259831845166777E-4	3.938974776775017E-4
24-25	9.847436941937541E-5	0.0	0.0	3.6107268787104326E-4	3.938974776775017E-4
26-27	1.3129915922583388E-4	0.0	0.0	5.251966369033355E-4	3.938974776775017E-4
28-29	1.3129915922583388E-4	0.0	0.0	0.001181692433032505	3.938974776775017E-4
30-31	1.3129915922583388E-4	0.0	0.0	0.004792419311742938	4.5954705729041865E-4
32-33	1.3129915922583388E-4	0.0	0.0	0.013195565502196306	5.908462165162525E-4
34-35	1.3129915922583388E-4	0.0	0.0	0.023601023870843646	5.908462165162525E-4
36-37	1.3129915922583388E-4	0.0	0.0	0.04004624356387934	5.908462165162525E-4
38-39	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.07044199892465988	5.908462165162525E-4
40-41	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.10589277191563504	5.908462165162525E-4
42-43	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.1444947247280302	5.908462165162525E-4
44-45	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.18979293466094288	6.23671006322711E-4
46-47	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.24142632902650207	7.221453757420864E-4
48-49	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.29854146328973985	7.221453757420864E-4
50-51	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.36153223492833364	7.221453757420864E-4
52-53	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.42409628429944346	8.206197451614619E-4
54-55	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.49266727020513523	8.534445349679203E-4
56-57	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.5784384459694112	8.534445349679203E-4
58-59	1.3129915922583388E-4	6.564957961291694E-5	0.0	0.6724486439751083	8.534445349679203E-4
60-61	1.3129915922583388E-4	1.3129915922583388E-4	0.0	0.7700039192799029	8.534445349679203E-4
62-63	1.9694873883875085E-4	1.3129915922583388E-4	0.0	0.8759295159853444	8.534445349679203E-4
64-65	1.9694873883875085E-4	1.3129915922583388E-4	0.0	0.9909147546773684	8.534445349679203E-4
66-67	2.2977352864520932E-4	1.3129915922583388E-4	0.0	1.1226078113808797	8.534445349679203E-4
68-69	3.2824789806458476E-4	1.3129915922583388E-4	0.0	1.2645422025040063	8.534445349679203E-4
70-71	3.2824789806458476E-4	1.3129915922583388E-4	0.0	1.4223966166832651	8.534445349679203E-4
72-73	3.2824789806458476E-4	1.3129915922583388E-4	0.0	1.587341185460719	9.190941145808373E-4
74-75	3.2824789806458476E-4	1.3129915922583388E-4	0.0	1.767549281498176	9.190941145808373E-4
76-77	3.2824789806458476E-4	1.3129915922583388E-4	0.0	1.9660736102476366	9.190941145808373E-4
78-79	3.2824789806458476E-4	1.3129915922583388E-4	0.0	2.195124993517104	9.190941145808373E-4
80-81	3.2824789806458476E-4	1.3129915922583388E-4	0.0	2.4387177586708324	9.847436941937543E-4
82-83	3.2824789806458476E-4	1.3129915922583388E-4	0.0	2.6958999868044344	0.001116042853419588
84-85	3.2824789806458476E-4	1.3129915922583388E-4	0.0	2.967131224975201	0.001116042853419588
86-87	3.2824789806458476E-4	1.3129915922583388E-4	0.0	3.2642940470930695	0.001116042853419588
88-89	3.2824789806458476E-4	1.3129915922583388E-4	0.0	3.5971702405203647	0.001116042853419588
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1390	0.0	50.27283	1
GTATCAA	2370	0.0	40.11552	1
TCAACGC	2985	0.0	30.998167	4
CAACGCA	3020	0.0	30.223125	5
ATCAACG	3155	0.0	29.3279	3
TATCAAC	3200	0.0	28.916428	2
AACGCAG	3290	0.0	27.740984	6
GTACATG	3750	0.0	25.86007	1
TACATGG	3885	0.0	24.428621	2
ACGCAGA	3830	0.0	23.581495	7
CGCAGAG	3850	0.0	22.841652	8
ACATGGG	4140	0.0	21.777046	3
CATGGGG	3560	0.0	20.393284	4
GTATATA	600	0.0	19.807041	1
AGAGTAC	3835	0.0	19.274431	10-11
GCAGAGT	4670	0.0	18.22018	9
ACCGTGC	345	1.0732037E-9	17.911863	8
GAGTACT	2455	0.0	17.681355	12-13
CAGAGTA	4255	0.0	17.539476	9
TCTATAC	460	0.0	17.536255	3
>>END_MODULE