FastQCFastQC Report
Mon 15 Aug 2016
SRR2921390_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921390_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188835
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.047.0114251
GTATCAA38300.042.6189771
TCAACGC45050.035.813554
CAACGCA46150.034.8531155
TATCAAC46850.034.3370742
ATCAACG47550.033.928283
AACGCAG48800.032.758536
ACGCAGA56200.028.3646137
CGCAGAG57150.027.0654328
GTACATG38600.022.8648851
GCAGAGT64100.022.5759229
CGTATAC1353.8247238E-421.0887533
GTGGTAT11400.020.3955121
TACATGG42500.020.3214932
ACATGGG44050.019.820083
GAGTACT40700.019.46908812-13
AGAGTAC55100.019.33206710-11
CAGAGTA59100.019.30817410-11
GTATATA8050.018.8624211
GTACTTT42900.018.3594414-15
Produced by FastQC (version 0.11.5)