##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921390_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2188835
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.492522734696767	31.0	31.0	34.0	26.0	34.0
2	30.560767257468015	31.0	31.0	34.0	26.0	34.0
3	30.60219568857406	33.0	31.0	34.0	26.0	34.0
4	34.080485280982806	37.0	35.0	37.0	30.0	37.0
5	34.0810513355278	37.0	35.0	37.0	30.0	37.0
6	34.13251204407824	37.0	35.0	37.0	30.0	37.0
7	34.05090105010199	37.0	35.0	37.0	30.0	37.0
8	34.0184449718686	37.0	35.0	37.0	30.0	37.0
9	35.58337380387284	39.0	37.0	39.0	30.0	39.0
10-11	35.485217021840384	39.0	36.0	39.0	30.0	39.0
12-13	35.37974173475844	39.0	35.0	39.0	30.0	39.0
14-15	36.51985919450301	40.0	37.0	41.0	29.0	41.0
16-17	36.47425000057108	40.0	37.0	41.0	29.0	41.0
18-19	36.445300810705234	40.0	37.0	41.0	29.0	41.0
20-21	36.390509334874494	40.0	37.0	41.0	28.5	41.0
22-23	36.27177631022896	40.0	36.5	41.0	28.0	41.0
24-25	36.1804724887897	40.0	36.0	41.0	27.0	41.0
26-27	36.032485774395965	40.0	36.0	41.0	27.0	41.0
28-29	35.88432682225933	40.0	36.0	41.0	27.0	41.0
30-31	35.72155096204145	40.0	36.0	41.0	27.0	41.0
32-33	35.5275404495999	39.0	35.0	41.0	26.0	41.0
34-35	35.34844974609781	39.0	35.0	41.0	25.0	41.0
36-37	35.16713091667485	39.0	35.0	41.0	25.0	41.0
38-39	35.36633643011008	39.5	35.0	41.0	25.0	41.0
40-41	35.46886288824877	40.0	35.0	41.0	25.0	41.0
42-43	35.282027653980315	40.0	35.0	41.0	24.0	41.0
44-45	35.14387311058166	40.0	35.0	41.0	24.0	41.0
46-47	34.96895083457638	40.0	35.0	41.0	23.0	41.0
48-49	34.81345715871686	39.5	35.0	41.0	22.0	41.0
50-51	33.08933953450123	37.0	32.5	39.5	18.0	40.0
52-53	33.24668396658497	37.5	33.0	39.5	17.0	40.0
54-55	34.12158728273259	39.0	34.0	40.5	18.5	41.0
56-57	34.14460797638927	39.0	34.0	41.0	18.0	41.0
58-59	33.9728362348007	38.5	34.0	41.0	17.5	41.0
60-61	33.73567765500826	38.0	34.0	40.5	14.0	41.0
62-63	33.457189783606346	37.5	34.0	40.0	12.5	41.0
64-65	33.169025531846856	37.0	33.0	40.0	9.5	41.0
66-67	32.82777276496401	36.5	33.0	40.0	7.5	41.0
68-69	32.465731542121716	36.0	33.0	39.0	4.5	41.0
70-71	32.08200526764237	35.5	32.0	39.0	2.0	41.0
72-73	31.716858739923293	35.0	32.0	38.5	2.0	40.0
74-75	31.30605915932448	35.0	32.0	37.0	2.0	39.5
76-77	30.917449236694406	35.0	31.5	37.0	2.0	39.0
78-79	30.54794742408633	35.0	31.0	36.0	2.0	39.0
80-81	30.1753962724463	35.0	31.0	36.0	2.0	37.5
82-83	29.84606720013158	35.0	31.0	35.5	2.0	37.0
84-85	29.584322938914994	35.0	31.0	35.0	2.0	37.0
86-87	29.335348027603725	35.0	30.5	35.0	2.0	36.0
88-89	29.10904088247858	35.0	30.0	35.0	2.0	36.0
90-91	28.83375128778551	34.0	30.0	35.0	2.0	36.0
92-93	28.624989092371056	34.0	30.0	35.0	2.0	35.0
94-95	28.400853193593854	34.0	30.0	35.0	2.0	35.0
96-97	28.130415038136725	34.0	29.0	35.0	2.0	35.0
98-99	27.874477518862776	34.0	29.0	35.0	2.0	35.0
100-101	26.80757115086336	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65944.0
3	11644.0
4	7904.0
5	6360.0
6	5857.0
7	6146.0
8	6553.0
9	7111.0
10	7975.0
11	8720.0
12	9104.0
13	9363.0
14	10544.0
15	11294.0
16	12230.0
17	12809.0
18	11711.0
19	10081.0
20	10288.0
21	10657.0
22	11578.0
23	12920.0
24	15134.0
25	17570.0
26	20822.0
27	24413.0
28	28628.0
29	33571.0
30	39789.0
31	48355.0
32	59023.0
33	76309.0
34	103678.0
35	150618.0
36	247449.0
37	447792.0
38	505989.0
39	102883.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77518684644881	19.045653566046354	13.407463984835625	24.771695602669215
2	16.086330856323688	20.09554833094409	39.016467481934406	24.801653330797812
3	19.084053206714238	23.340306951967964	30.165250536048365	27.410389305269433
4	13.442365016609367	16.116731371658986	36.71833466583105	33.72256894590059
5	14.157653832195361	36.64973036914608	34.913424682078755	14.279191116579806
6	33.159249720802116	35.468686883457046	17.084534356419177	14.287529039321663
7	28.179641484690215	32.02387969677446	21.30753448756437	18.488944330970952
8	25.78450838418988	34.834224036886326	20.34647187714917	19.03479570177463
9	25.925252324926234	15.860130171192985	20.297402288209057	37.917215215671725
10-11	24.893458249708456	25.533234262986078	28.57553474225259	20.99777274505288
12-13	25.486933495170366	23.225792594356253	28.05033064704271	23.236943263430675
14-15	22.783861269721196	25.21906855726589	25.49513961813979	26.50193055487312
16-17	22.646367818707862	28.016436317837055	26.61208763537935	22.725108228075733
18-19	22.61568670818398	27.236391868845743	28.056882801632664	22.091038621337606
20-21	23.074929885902645	26.556551240986394	28.07826821721226	22.290250655898703
22-23	22.99262474411946	26.448351840225868	27.64359991836505	22.915423497289613
24-25	22.804547823978524	26.575692209459877	27.75307503278824	22.86668493377336
26-27	22.698660897395033	26.727531020003475	27.74220349707252	22.83160458552897
28-29	22.806985877494675	26.91732013517722	27.666547933831616	22.60914605349649
30-31	23.10780901171265	26.562204430830306	27.42450106781859	22.90548548963846
32-33	22.79297857967405	26.711840725233944	27.31148100503257	23.183699690059445
34-35	23.2549506166268	26.69987295772286	27.284664939415613	22.760511486234726
36-37	23.217630247351696	26.478859597684856	27.397510926616857	22.90599922834659
38-39	22.950640286415545	26.652321826392555	27.4333305310713	22.963707356120597
40-41	23.217427576606127	26.531882649590337	27.40872575854559	22.841964015257947
42-43	23.187177093173457	26.53931799147661	27.1277908559701	23.145714059379827
44-45	23.10865252629432	26.573384796982495	27.222013550751456	23.095949125971725
46-47	23.218489364682746	26.604339279027034	26.950143888778506	23.227027467511718
48-49	23.031884917828204	26.68357301155885	26.946232512828544	23.338309557784402
50-51	23.099727891654734	26.77758530395198	27.00808084877295	23.114605955620338
52-53	23.266107194049955	26.630359393476873	26.96318623790756	23.14034717456562
54-55	22.987502002884153	26.567468583331426	27.146748460640463	23.29828095314396
56-57	22.955467246063215	26.697446536039944	27.04568883044661	23.30139738745023
58-59	22.959704222782452	26.54323997470025	27.159022832724762	23.338032969792533
60-61	22.897589081638632	26.543269794291742	27.29601788225986	23.26312324180976
62-63	22.932087015464436	26.573450746414895	27.237350344448995	23.257111893671674
64-65	22.79784692253825	26.49151526958452	27.126138811824113	23.584498996053117
66-67	22.736318521841838	26.494662003168006	27.02328849904319	23.74573097594697
68-69	22.869862468102873	26.332537679125203	27.16438087756356	23.63321897520837
70-71	22.747630068995253	26.190572639778498	27.156373449998412	23.90542384122784
72-73	22.766135339350868	26.22294049535364	27.13424508041778	23.876679084877715
74-75	22.73427956788424	26.40072673889653	27.07850826267244	23.78648543054679
76-77	22.7085215834155	26.183629543979336	27.113253080435086	23.994595792170077
78-79	22.624132423180377	26.194392609803398	27.150859066170614	24.030615900845618
80-81	22.691893287884692	26.132961086737822	27.133782111191344	24.04136351418614
82-83	22.65101662248626	26.187456184012724	27.153525724534983	24.008001468966032
84-85	22.67717653092061	26.16127437064442	27.16753358547239	23.994015512962573
86-87	22.64270316834213	26.14623018898859	27.076541133766664	24.13452550890262
88-89	22.479716673417663	25.966916991603657	27.168792676959473	24.38457365801921
90-91	22.46578046281841	25.934605867959686	27.20293494154892	24.39667872767298
92-93	22.5359601403852	25.710884982277822	27.386975464647584	24.366179412689394
94-95	22.443343621203624	25.572277575091647	27.4303461867971	24.554032616907634
96-97	22.441801322161652	25.36956930470749	27.49637764714585	24.69225172598501
98-99	22.35639100774512	25.378989800180257	27.35467821473845	24.909940977336177
100-101	22.45485525950282	25.131948161038704	27.53581270151193	24.87738387794655
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	187.0
1	133.5
2	122.5
3	172.5
4	165.5
5	186.5
6	282.0
7	535.5
8	789.0
9	868.5
10	776.5
11	638.5
12	670.0
13	734.5
14	809.0
15	937.5
16	1006.5
17	1114.5
18	1243.0
19	1409.5
20	1630.5
21	1815.5
22	2097.5
23	2452.5
24	2920.5
25	3774.5
26	5017.5
27	6626.0
28	8999.0
29	12296.5
30	16427.5
31	21785.5
32	28301.5
33	32961.0
34	36116.0
35	40584.0
36	46406.0
37	52457.5
38	57936.0
39	62731.0
40	67012.0
41	71210.5
42	75316.5
43	79001.5
44	82653.5
45	87185.0
46	90111.0
47	91495.5
48	92635.0
49	92565.5
50	90450.5
51	87777.5
52	85699.0
53	83841.0
54	80899.5
55	75714.0
56	69147.0
57	61037.0
58	54306.5
59	47647.5
60	39040.5
61	31592.5
62	25754.5
63	19470.0
64	13503.5
65	9762.0
66	7275.5
67	5402.5
68	3955.0
69	2914.0
70	2207.0
71	1642.5
72	1201.0
73	903.5
74	669.5
75	474.5
76	338.0
77	217.5
78	156.5
79	117.5
80	75.0
81	49.5
82	43.5
83	47.5
84	46.0
85	42.0
86	36.0
87	27.5
88	24.5
89	21.0
90	16.5
91	16.5
92	13.0
93	7.5
94	5.0
95	7.5
96	9.5
97	6.5
98	4.0
99	4.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04915857065516588
2	0.05715369134722352
3	0.048701706615619726
4	0.055463294400902766
5	0.047011309669298965
6	0.019325348872802198
7	0.016675537443434522
8	0.022386337937761413
9	0.022203592321942953
10-11	0.02243202434171603
12-13	0.02857684567361176
14-15	0.045343755924955514
16-17	0.028873807299316757
18-19	0.02334575242080833
20-21	0.02814282483604292
22-23	0.047810821738504726
24-25	0.042556885283723984
26-27	0.03154646193066175
28-29	0.020490352173644883
30-31	0.011924151432154547
32-33	0.008657573549399568
34-35	0.008840319165218026
36-37	0.0013020625127065312
38-39	0.005802173302236121
40-41	0.003380793892641519
42-43	0.22669593642280025
44-45	0.2001292925231916
46-47	0.20599999543135958
48-49	0.20321312479012807
50-51	0.20152272784380731
52-53	0.20631980025904192
54-55	0.20490352173644882
56-57	0.21579972907962455
58-59	0.21123108868416307
60-61	0.21796983326746877
62-63	0.21447482336494073
64-65	0.21497737380844145
66-67	0.205360385775995
68-69	0.20417253927317502
70-71	0.18820514109103703
72-73	0.19528653370400234
74-75	0.19560633853168466
76-77	0.19375603917152276
78-79	0.2195917006078576
80-81	0.2274269188860741
82-83	0.22779241011771104
84-85	0.22180749119965643
86-87	0.24049323041709403
88-89	0.23786626218970366
90-91	0.2382089102193633
92-93	0.24083587844675364
94-95	0.25216610662749817
96-97	0.25202904741563437
98-99	0.24063028962895786
100-101	0.251115319336542
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2188835.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.13186550328611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.77031404304398	51.158190513633926
2	11.629932959889066	14.916985927916796
3	3.6556420539144248	7.0332943358938955
4	1.61769524315831	4.149832550381379
5	0.9162054334959023	2.9378981817169576
6	0.5837763521141243	2.2463199898669206
7	0.3734881273934082	1.676674324714777
8	0.26856248599758425	1.3778730584980188
9	0.20565910627511544	1.1870371928845558
>10	0.9385047127788969	10.099074043871525
>50	0.029417547508867055	1.2659059790837468
>100	0.009444421209414789	1.1668711731376527
>500	0.0010717209637911636	0.49972356939565427
>1k	2.8579225701090934E-4	0.2843191590041716
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.568640395461513E-5
2	0.0	0.0	0.0	4.568640395461513E-5	4.568640395461513E-5
3	0.0	0.0	0.0	4.568640395461513E-5	4.568640395461513E-5
4	0.0	0.0	0.0	4.568640395461513E-5	4.568640395461513E-5
5	0.0	0.0	0.0	4.568640395461513E-5	4.568640395461513E-5
6	0.0	0.0	0.0	4.568640395461513E-5	9.137280790923026E-5
7	0.0	0.0	0.0	4.568640395461513E-5	9.137280790923026E-5
8	0.0	0.0	0.0	4.568640395461513E-5	9.137280790923026E-5
9	0.0	0.0	0.0	4.568640395461513E-5	9.137280790923026E-5
10-11	0.0	0.0	0.0	9.137280790923026E-5	9.137280790923026E-5
12-13	0.0	0.0	0.0	9.137280790923026E-5	1.1421600988653782E-4
14-15	0.0	0.0	0.0	9.137280790923026E-5	1.8274561581846052E-4
16-17	0.0	0.0	0.0	1.5990241384115293E-4	2.2843201977307563E-4
18-19	0.0	0.0	0.0	2.2843201977307563E-4	2.2843201977307563E-4
20-21	0.0	0.0	0.0	2.2843201977307563E-4	2.5127522175038316E-4
22-23	0.0	0.0	0.0	5.025504435007664E-4	3.6549123163692103E-4
24-25	0.0	0.0	0.0	7.766688672284571E-4	4.1117763559153615E-4
26-27	0.0	0.0	0.0	0.0014848081285249917	4.5686403954615126E-4
28-29	0.0	0.0	0.0	0.0049112884251211264	5.025504435007664E-4
30-31	0.0	0.0	0.0	0.018502993601619125	5.25393645478074E-4
32-33	0.0	0.0	0.0	0.04945553228087087	5.482368474553815E-4
34-35	0.0	0.0	0.0	0.09482213140780368	5.482368474553815E-4
36-37	0.0	0.0	0.0	0.16170702679736024	5.482368474553815E-4
38-39	0.0	0.0	0.0	0.2508640441147917	5.939232514099967E-4
40-41	0.0	0.0	0.0	0.34813039813416724	5.939232514099967E-4
42-43	0.0	0.0	0.0	0.4590569869359728	5.939232514099967E-4
44-45	0.0	0.0	0.0	0.6137511507262996	5.939232514099967E-4
46-47	0.0	0.0	0.0	0.790397631616819	6.167664533873042E-4
48-49	0.0	0.0	0.0	0.9570387900412777	7.081392612965345E-4
50-51	0.0	0.0	0.0	1.1209616074304367	7.309824632738421E-4
52-53	0.0	0.0	0.0	1.3016284918689622	7.766688672284571E-4
54-55	2.2843201977307564E-5	0.0	0.0	1.5238471607042103	7.766688672284571E-4
56-57	4.568640395461513E-5	0.0	0.0	1.7867952586649976	7.766688672284571E-4
58-59	4.568640395461513E-5	0.0	0.0	2.053443955346109	7.766688672284571E-4
60-61	4.568640395461513E-5	0.0	0.0	2.318607843898695	7.766688672284571E-4
62-63	4.568640395461513E-5	0.0	0.0	2.5955359814695944	7.766688672284571E-4
64-65	4.568640395461513E-5	0.0	0.0	2.9245237763467777	7.766688672284571E-4
66-67	4.568640395461513E-5	0.0	0.0	3.3230919644468404	7.766688672284571E-4
68-69	4.568640395461513E-5	0.0	0.0	3.7482267964465117	7.766688672284571E-4
70-71	4.568640395461513E-5	0.0	0.0	4.174229670121321	8.223552711830723E-4
72-73	4.568640395461513E-5	0.0	0.0	4.61167698798676	8.680416751376874E-4
74-75	4.568640395461513E-5	0.0	0.0	5.094216786555405	8.680416751376874E-4
76-77	4.568640395461513E-5	0.0	0.0	5.659974369927381	8.680416751376874E-4
78-79	4.568640395461513E-5	0.0	0.0	6.275461604003956	8.680416751376874E-4
80-81	4.568640395461513E-5	0.0	0.0	6.903032891926527	8.680416751376874E-4
82-83	4.568640395461513E-5	0.0	0.0	7.528479762065208	9.594144830469177E-4
84-85	4.568640395461513E-5	0.0	0.0	8.202491279607646	9.594144830469177E-4
86-87	4.568640395461513E-5	0.0	0.0	8.963763828703396	0.0010279440889788404
88-89	4.568640395461513E-5	0.0	0.0	9.783948995698625	0.001050787290956148
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2160	0.0	47.011425	1
GTATCAA	3830	0.0	42.618977	1
TCAACGC	4505	0.0	35.81355	4
CAACGCA	4615	0.0	34.853115	5
TATCAAC	4685	0.0	34.337074	2
ATCAACG	4755	0.0	33.92828	3
AACGCAG	4880	0.0	32.75853	6
ACGCAGA	5620	0.0	28.364613	7
CGCAGAG	5715	0.0	27.065432	8
GTACATG	3860	0.0	22.864885	1
GCAGAGT	6410	0.0	22.575922	9
CGTATAC	135	3.8247238E-4	21.088753	3
GTGGTAT	1140	0.0	20.395512	1
TACATGG	4250	0.0	20.321493	2
ACATGGG	4405	0.0	19.82008	3
GAGTACT	4070	0.0	19.469088	12-13
AGAGTAC	5510	0.0	19.332067	10-11
CAGAGTA	5910	0.0	19.308174	10-11
GTATATA	805	0.0	18.862421	1
GTACTTT	4290	0.0	18.35944	14-15
>>END_MODULE