FastQCFastQC Report
Mon 15 Aug 2016
SRR2921390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188835
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22850.10439343303629556No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20800.054.154941
GTATCAA38750.042.683561
CAACGCG550.002567966234.5787669
TCAACGC47400.034.541274
ATCAACG49250.033.0510673
CAACGCA50150.032.709985
TATCAAC49950.032.6828962
AACGCAG52900.030.918966
ACGCAGA61700.026.35447
CGCAGAG62350.025.6983748
GCAGAGT72400.021.2774039
GTACATG39000.021.2049751
TACATGG41750.019.892032
GTGGTAT10400.019.6511611
AGAGTAC62600.019.48162710-11
GAGTACT45800.018.54325112-13
CAGAGTA66800.018.50585210-11
ACATGGG44550.018.1091793
TATACTG7950.017.9426355
TGGTATC10750.017.658312
Produced by FastQC (version 0.11.5)