##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921390_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2188835
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.506850447840975	33.0	31.0	34.0	30.0	34.0
2	31.636136118071942	34.0	31.0	34.0	30.0	34.0
3	31.71088090239785	34.0	31.0	34.0	29.0	34.0
4	35.214661223893074	37.0	35.0	37.0	33.0	37.0
5	34.96803733492931	37.0	35.0	37.0	32.0	37.0
6	34.92872327059828	37.0	35.0	37.0	32.0	37.0
7	34.79636336224521	37.0	35.0	37.0	32.0	37.0
8	34.76611850596322	37.0	35.0	37.0	32.0	37.0
9	36.39424259937364	39.0	37.0	39.0	32.0	39.0
10-11	36.250958614970976	39.0	37.0	39.0	32.0	39.0
12-13	36.19779540257717	39.0	37.0	39.0	32.0	39.0
14-15	37.44528344987174	40.0	38.0	41.0	32.0	41.0
16-17	37.430180895316454	40.0	38.0	41.0	32.0	41.0
18-19	37.40925309582495	40.0	38.0	41.0	32.0	41.0
20-21	37.37329903807276	40.0	38.0	41.0	32.0	41.0
22-23	37.26062974139211	40.0	38.0	41.0	31.0	41.0
24-25	37.20041094920357	40.0	38.0	41.0	31.0	41.0
26-27	37.06469263329579	40.0	37.0	41.0	31.0	41.0
28-29	36.9069073274139	40.0	37.0	41.0	30.0	41.0
30-31	36.75273079058038	40.0	37.0	41.0	30.0	41.0
32-33	36.67633010254313	40.0	37.0	41.0	30.0	41.0
34-35	36.784530126756934	40.0	37.0	41.0	30.0	41.0
36-37	36.73802159596315	40.0	37.0	41.0	30.0	41.0
38-39	36.640113804832254	40.0	37.0	41.0	30.0	41.0
40-41	36.50271080277865	40.0	37.0	41.0	30.0	41.0
42-43	36.37735553387989	40.0	36.5	41.0	29.5	41.0
44-45	36.22031011931004	40.0	36.0	41.0	29.0	41.0
46-47	36.04423152042068	40.0	36.0	41.0	28.0	41.0
48-49	35.867801364652884	40.0	36.0	41.0	27.5	41.0
50-51	35.708079640539374	40.0	35.0	41.0	27.0	41.0
52-53	35.5404644479826	39.0	35.0	41.0	27.0	41.0
54-55	35.33498938933268	39.0	35.0	41.0	26.0	41.0
56-57	35.06816594215644	39.0	35.0	41.0	26.0	41.0
58-59	34.85003369372292	39.0	35.0	41.0	26.0	41.0
60-61	34.5875150479593	38.0	34.5	40.0	25.0	41.0
62-63	34.30403525162929	38.0	34.0	40.0	24.5	41.0
64-65	33.99188381033746	37.0	34.0	40.0	23.5	41.0
66-67	33.65747372460693	37.0	34.0	39.5	23.0	41.0
68-69	33.295334504428155	36.0	34.0	39.0	22.0	41.0
70-71	32.91743758666139	35.5	33.0	39.0	22.0	40.5
72-73	32.5176744706659	35.0	33.0	38.0	20.5	40.0
74-75	32.1052772365208	35.0	33.0	37.0	19.5	39.5
76-77	29.302931239677726	33.0	29.0	34.5	12.0	36.5
78-79	30.963056831602202	34.5	31.5	36.0	15.0	38.0
80-81	31.151770005505213	35.0	32.0	36.0	17.0	37.0
82-83	30.972191599640905	35.0	32.0	35.5	13.5	37.0
84-85	30.727044523684974	35.0	32.0	35.0	9.5	37.0
86-87	30.468029568240638	35.0	32.0	35.0	7.0	36.0
88-89	30.170799991776448	35.0	31.5	35.0	2.0	36.0
90-91	29.975997048658304	34.5	31.0	35.0	2.0	35.5
92-93	29.825566568517043	34.0	31.0	35.0	2.0	35.0
94-95	29.727016654978563	34.0	31.0	35.0	2.0	35.0
96-97	29.53007832933958	34.0	31.0	35.0	2.0	35.0
98-99	29.256590377986463	34.0	31.0	35.0	2.0	35.0
100-101	28.1652299054063	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34073.0
3	11960.0
4	6669.0
5	4720.0
6	4277.0
7	4459.0
8	4780.0
9	5151.0
10	5879.0
11	6670.0
12	7192.0
13	8130.0
14	9074.0
15	10338.0
16	11476.0
17	12827.0
18	11494.0
19	9598.0
20	9012.0
21	8364.0
22	8854.0
23	10065.0
24	11627.0
25	13681.0
26	16530.0
27	19902.0
28	23733.0
29	28564.0
30	34718.0
31	42628.0
32	54185.0
33	70863.0
34	99717.0
35	148612.0
36	256487.0
37	502200.0
38	575053.0
39	85270.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.3254185106088	20.392997054421137	13.896890554150465	25.384693880819597
2	15.300650304136198	21.469904816380684	37.60721520525653	25.622229674226592
3	17.820953895216817	24.411000086809125	30.042764916320575	27.725281101653486
4	13.19062439462998	17.28452482336902	35.7560774581221	33.7687733238789
5	13.927350780832642	37.14408056516627	34.82984735227975	14.098721301721337
6	33.12857873198955	36.08883227742703	16.75041883699691	14.03217015358651
7	28.07024196444317	32.26882440605364	21.278498049646526	18.382435579856672
8	25.840285455824706	35.321686053417885	20.09489304544084	18.743135445316568
9	25.8266612147558	15.860537683288142	20.220025721445424	38.09277538051064
10-11	24.953429436620812	25.84754309120604	28.405139432701247	20.7938880394719
12-13	25.468989461714337	23.45528669788428	28.042146746535874	23.03357709386551
14-15	22.674779202284085	25.417831450147727	25.48124775695655	26.426141590611646
16-17	22.623672292353582	28.360529762717874	26.484229695421618	22.53156824950693
18-19	22.554970741131022	27.419844496369855	28.13203074619767	21.89315401630145
20-21	23.056010774799653	26.60197705512262	28.174131983072087	22.16788018700564
22-23	22.934621936234898	26.49293766694132	27.756489138834645	22.81595125798914
24-25	22.782423404271846	26.682853640607146	27.845661338168863	22.689061616952145
26-27	22.628092388390527	26.874998715038483	27.850701406239043	22.64620749033195
28-29	22.766849941198025	27.040136117205538	27.736941803863658	22.456072137732775
30-31	23.078032014446347	26.690052419177963	27.479104523987075	22.75281104238862
32-33	22.753009409824624	26.793131675630526	27.394865137727237	23.058993776817612
34-35	23.23222607840212	26.79773667548453	27.37092427156696	22.599112974546394
36-37	23.189001379738855	26.62907411299239	27.42651291563491	22.755411591633848
38-39	22.966152724375373	26.713722204353736	27.529522594698907	22.79060247657198
40-41	23.19823843291987	26.652222998419635	27.437608881485314	22.711929687175182
42-43	23.203625098806103	26.61781571119833	27.120870133371106	23.057689056624465
44-45	23.095605085224737	26.69605915415156	27.16646231737353	23.04187344325018
46-47	23.19214215220411	26.791295050807808	26.94611722348125	23.070445573506834
48-49	22.983016741551037	26.838592678001632	26.997324541092887	23.181066039354445
50-51	23.089169464541857	26.921852987221495	26.953218430270372	23.035759117966276
52-53	23.31203556278426	26.750669886079393	26.815728216995115	23.12156633414123
54-55	22.941718262293403	26.708138098405716	26.98648493066571	23.36365870863517
56-57	22.95680866554318	26.84943605024776	26.904079997642448	23.289675286566613
58-59	22.949112620696035	26.671639387144563	26.980877110217342	23.398370881942057
60-61	22.873314626185635	26.679708212358644	27.14536129978048	23.30161586167524
62-63	22.892816817162615	26.676303707999015	27.08145698904832	23.349422485790047
64-65	22.76400024222564	26.574849437339537	26.937799425742416	23.723350894692402
66-67	22.710263608619297	26.698750798389852	26.847745484169888	23.743240108820967
68-69	22.787207614632614	26.618996000380218	26.866344515695033	23.727451869292132
70-71	22.733290324084514	26.460240554705045	26.868439918341846	23.938029202868595
72-73	22.783523905317384	26.48972666123459	26.785307075970287	23.941442357477733
74-75	22.680703049972685	26.650742334511058	26.725596139956508	23.942958475559752
76-77	22.69711094327732	26.38951037773138	26.873348029222623	24.040030649768685
78-79	22.569336891855592	26.463769938106857	26.880231342046763	24.08666182799079
80-81	22.697810575328226	26.448057132160635	26.746256621608	24.10787567090314
82-83	22.59739796843948	26.491237530085222	26.811293535316516	24.100070966158786
84-85	22.555047758329145	26.47820026507015	26.784287738220375	24.18246423838033
86-87	22.519481006418832	26.497392320628876	26.71065229935775	24.272474373594537
88-89	22.333947707708663	26.351736239392075	26.765597203271952	24.54871884962731
90-91	22.253461863014227	26.25934992925245	26.815567302760552	24.671620904972773
92-93	22.2918862236923	26.153301486279645	26.946346605376974	24.608465684651087
94-95	22.218607744176584	26.016223574554814	26.959625188779697	24.805543492488905
96-97	22.11129611065369	25.886208223178897	26.97702269128034	25.025472974887077
98-99	22.012449744152047	25.919750548245613	26.876256396198826	25.191543311403507
100-101	21.89651930997035	25.914420406208045	26.93454349166406	25.254516792157546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	178.0
1	133.0
2	111.0
3	144.5
4	138.5
5	150.5
6	241.5
7	475.0
8	706.5
9	743.5
10	641.0
11	528.0
12	494.5
13	501.5
14	564.0
15	659.5
16	744.0
17	831.5
18	947.0
19	1069.5
20	1239.5
21	1517.0
22	1790.5
23	2178.5
24	2720.5
25	3523.0
26	4777.0
27	6638.5
28	9139.5
29	12643.0
30	16930.0
31	22022.0
32	28724.5
33	33631.0
34	36631.0
35	41016.0
36	46935.5
37	52923.5
38	58096.0
39	62782.0
40	67364.0
41	71647.5
42	75867.0
43	80095.0
44	83563.5
45	88050.0
46	91243.0
47	92242.5
48	93554.0
49	93693.5
50	91610.5
51	88803.5
52	86181.5
53	84127.0
54	81113.5
55	75599.5
56	68540.0
57	60702.5
58	53954.0
59	46552.0
60	37803.0
61	30697.5
62	25033.5
63	18595.5
64	12659.5
65	9030.5
66	6711.0
67	5048.5
68	3652.5
69	2641.0
70	1972.5
71	1439.5
72	1000.0
73	740.5
74	553.5
75	378.0
76	243.5
77	136.0
78	81.0
79	51.0
80	28.5
81	19.5
82	13.5
83	5.5
84	4.0
85	4.0
86	4.0
87	2.5
88	2.0
89	1.0
90	1.0
91	1.5
92	1.0
93	0.5
94	0.5
95	1.0
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16109026034397295
2	5.939232514099967E-4
3	0.0057108004943268904
4	0.004157462759869977
5	0.027137723949041383
6	0.0016447105423661446
7	4.568640395461512E-5
8	0.002512752217503832
9	0.0
10-11	0.1081854045645286
12-13	2.9696162570499833E-4
14-15	0.005642270888394968
16-17	2.741184237276908E-4
18-19	5.25393645478074E-4
20-21	0.0010964736949107632
22-23	2.0558881779576807E-4
24-25	0.0017360833502753747
26-27	0.0024442226115719092
28-29	0.006076291725963812
30-31	0.014756708477340686
32-33	0.014825238083272609
34-35	0.012997781925088003
36-37	6.852960593192269E-4
38-39	1.370592118638454E-4
40-41	0.004660013203370743
42-43	0.0093657128106961
44-45	0.008246395913808031
46-47	0.00890884877114995
48-49	0.010896207343175706
50-51	0.005505211676531123
52-53	0.0013705921186384538
54-55	8.680416751376874E-4
56-57	0.005505211676531123
58-59	0.010393656899674942
60-61	0.008611887145444952
62-63	0.01845730719766451
64-65	0.03620647513403249
66-67	0.03826236331199017
68-69	0.02864537527954368
70-71	0.042648258091633216
72-73	0.027320469564859843
74-75	0.026018407052153317
76-77	0.24935639278428934
78-79	0.07298403031749766
80-81	0.055074959967288534
82-83	0.02138123705075988
84-85	0.03357950690664212
86-87	0.08374317844880953
88-89	0.26751673835624884
90-91	0.32592680581222433
92-93	0.054435350311923925
94-95	0.0048656020211665105
96-97	0.011010423353062246
98-99	0.0015990241384115293
100-101	0.0013934353206157613
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2188835.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.475442868932916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3029348033911	50.33788907451363
2	11.903552625813075	15.111665492742684
3	3.762130233874826	7.164086481774203
4	1.6813943233243245	4.269089972412851
5	0.879351677037909	2.790861856876007
6	0.546947041534886	2.0830623412367726
7	0.39134625485780355	1.738861378953816
8	0.2853306536117724	1.4489191681671478
9	0.20815640633753113	1.1891538070452308
>10	0.9971252495414624	10.596620142759942
>50	0.03030294170364578	1.2734789432270066
>100	0.010127472672691179	1.2217366293161476
>500	0.0010113571215497005	0.4677162000913233
>1k	2.8895917758545435E-4	0.3068585108833135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2285	0.10439343303629556	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.568640395461513E-5
2	0.0	0.0	0.0	0.0	4.568640395461513E-5
3	0.0	0.0	0.0	0.0	4.568640395461513E-5
4	0.0	0.0	0.0	0.0	4.568640395461513E-5
5	0.0	0.0	0.0	0.0	9.137280790923026E-5
6	0.0	0.0	0.0	0.0	9.137280790923026E-5
7	0.0	0.0	0.0	0.0	1.3705921186384537E-4
8	0.0	0.0	0.0	0.0	1.3705921186384537E-4
9	0.0	0.0	0.0	4.568640395461513E-5	2.2843201977307563E-4
10-11	0.0	0.0	0.0	1.3705921186384537E-4	2.2843201977307563E-4
12-13	0.0	0.0	0.0	1.3705921186384537E-4	3.1980482768230586E-4
14-15	0.0	0.0	0.0	1.3705921186384537E-4	3.6549123163692103E-4
16-17	0.0	0.0	0.0	1.3705921186384537E-4	4.1117763559153615E-4
18-19	0.0	0.0	0.0	1.3705921186384537E-4	4.1117763559153615E-4
20-21	0.0	0.0	0.0	1.3705921186384537E-4	5.025504435007664E-4
22-23	0.0	0.0	0.0	4.5686403954615126E-4	5.25393645478074E-4
24-25	0.0	0.0	0.0	7.081392612965345E-4	5.939232514099967E-4
26-27	0.0	0.0	0.0	0.0013934353206157615	5.939232514099967E-4
28-29	0.0	0.0	0.0	0.0049569748290757415	5.939232514099967E-4
30-31	0.0	0.0	0.0	0.0187314256213922	6.167664533873042E-4
32-33	0.0	0.0	0.0	0.05032357395600856	6.396096553646117E-4
34-35	4.568640395461513E-5	0.0	0.0	0.09660390116203368	6.396096553646117E-4
36-37	4.568640395461513E-5	0.0	0.0	0.1643339950247506	6.396096553646117E-4
38-39	4.568640395461513E-5	0.0	0.0	0.25392503317975085	6.396096553646117E-4
40-41	4.568640395461513E-5	0.0	0.0	0.35272188173160607	6.396096553646117E-4
42-43	4.568640395461513E-5	0.0	0.0	0.4653160242777551	6.396096553646117E-4
44-45	4.568640395461513E-5	0.0	0.0	0.6220432330440623	6.396096553646117E-4
46-47	4.568640395461513E-5	0.0	0.0	0.7998547172354243	6.396096553646117E-4
48-49	4.568640395461513E-5	0.0	0.0	0.9682319590101582	6.396096553646117E-4
50-51	4.568640395461513E-5	0.0	0.0	1.1328629156606138	6.396096553646117E-4
52-53	4.568640395461513E-5	0.0	0.0	1.3140551937446177	7.766688672284571E-4
54-55	4.568640395461513E-5	0.0	0.0	1.536685040215457	8.90884877114995E-4
56-57	4.568640395461513E-5	0.0	0.0	1.8013463783245425	9.3657128106961E-4
58-59	4.568640395461513E-5	0.0	0.0	2.070896161656772	9.594144830469177E-4
60-61	4.568640395461513E-5	0.0	0.0	2.3380930951853385	9.594144830469177E-4
62-63	4.568640395461513E-5	0.0	0.0	2.6174426121658323	0.0010051008870015329
64-65	4.568640395461513E-5	0.0	0.0	2.9509305178325453	0.0010051008870015329
66-67	4.568640395461513E-5	0.0	0.0	3.3522855765738395	0.0010051008870015329
68-69	4.568640395461513E-5	0.0	0.0	3.779681885569264	0.0010051008870015329
70-71	4.568640395461513E-5	0.0	0.0	4.209134082742646	0.001050787290956148
72-73	4.568640395461513E-5	0.0	0.0	4.650647490560047	0.0011878465028199933
74-75	4.568640395461513E-5	4.568640395461513E-5	0.0	5.138281323169632	0.0011878465028199933
76-77	4.568640395461513E-5	4.568640395461513E-5	0.0	5.710457846297231	0.0011878465028199933
78-79	4.568640395461513E-5	4.568640395461513E-5	0.0	6.335356479588457	0.0011878465028199933
80-81	4.568640395461513E-5	4.568640395461513E-5	0.0	6.973938190864089	0.0012106897047973009
82-83	4.568640395461513E-5	4.568640395461513E-5	0.0	7.612200097312041	0.0012335329067746084
84-85	4.568640395461513E-5	4.568640395461513E-5	0.0	8.300397243282386	0.0012335329067746084
86-87	4.568640395461513E-5	4.568640395461513E-5	0.0	9.07411933745577	0.0012335329067746084
88-89	4.568640395461513E-5	4.568640395461513E-5	0.0	9.907347972780041	0.0012335329067746084
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2080	0.0	54.15494	1
GTATCAA	3875	0.0	42.68356	1
CAACGCG	55	0.0025679662	34.578766	9
TCAACGC	4740	0.0	34.54127	4
ATCAACG	4925	0.0	33.051067	3
CAACGCA	5015	0.0	32.70998	5
TATCAAC	4995	0.0	32.682896	2
AACGCAG	5290	0.0	30.91896	6
ACGCAGA	6170	0.0	26.3544	7
CGCAGAG	6235	0.0	25.698374	8
GCAGAGT	7240	0.0	21.277403	9
GTACATG	3900	0.0	21.204975	1
TACATGG	4175	0.0	19.89203	2
GTGGTAT	1040	0.0	19.651161	1
AGAGTAC	6260	0.0	19.481627	10-11
GAGTACT	4580	0.0	18.543251	12-13
CAGAGTA	6680	0.0	18.505852	10-11
ACATGGG	4455	0.0	18.109179	3
TATACTG	795	0.0	17.942635	5
TGGTATC	1075	0.0	17.65831	2
>>END_MODULE