FastQCFastQC Report
Mon 15 Aug 2016
SRR2921389_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921389_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3665988
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40770.1112114933273104No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32350.050.0191271
GTATCAA61450.044.015841
TCAACGC71950.036.7400474
CAACGCA74150.035.711555
ATCAACG75800.035.058923
TATCAAC77850.034.1971472
AACGCAG78050.033.8575366
ACGCAGA91700.028.5099747
CGCAGAG93950.027.4755428
GTACATG79500.026.382211
TACATGG82750.024.6022592
ACATGGG83200.023.7273773
GCAGAGT108550.022.5561039
CATGGGG61300.021.6776834
GAGTACA61500.020.678341
AGAGTAC91350.019.47927710-11
GAGTACT64850.019.3175912-13
CAGAGTA101800.018.62168910-11
GTATAGC12600.017.7004131
AGTACAT67300.017.3462542
Produced by FastQC (version 0.11.5)