##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921389_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3665988
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.544656447320612	31.0	31.0	34.0	27.0	34.0
2	30.605218293131347	31.0	31.0	34.0	27.0	34.0
3	30.653678080779315	33.0	31.0	34.0	26.0	34.0
4	34.13900781999286	37.0	35.0	37.0	30.0	37.0
5	34.14305175030579	37.0	35.0	37.0	30.0	37.0
6	34.19062746522902	37.0	35.0	37.0	30.0	37.0
7	34.11192098828474	37.0	35.0	37.0	30.0	37.0
8	34.07741023702205	37.0	35.0	37.0	30.0	37.0
9	35.65137419980644	39.0	37.0	39.0	30.0	39.0
10-11	35.54633512166434	39.0	37.0	39.0	30.0	39.0
12-13	35.4462274562819	39.0	36.0	39.0	30.0	39.0
14-15	36.59113245869872	40.0	37.0	41.0	30.0	41.0
16-17	36.55231154602798	40.0	37.0	41.0	30.0	41.0
18-19	36.52392642856441	40.0	37.0	41.0	29.0	41.0
20-21	36.472939491345855	40.0	37.0	41.0	29.0	41.0
22-23	36.35761287270989	40.0	37.0	41.0	28.5	41.0
24-25	36.26822482779539	40.0	36.5	41.0	28.0	41.0
26-27	36.12435242559441	40.0	36.0	41.0	27.0	41.0
28-29	35.98784052211846	40.0	36.0	41.0	27.0	41.0
30-31	35.83218766673541	40.0	36.0	41.0	27.0	41.0
32-33	35.63805432532785	40.0	35.5	41.0	26.5	41.0
34-35	35.46728508112956	39.0	35.0	41.0	25.5	41.0
36-37	35.29160842861461	39.0	35.0	41.0	25.0	41.0
38-39	35.49142727690325	39.5	35.0	41.0	25.5	41.0
40-41	35.59566766175995	40.0	35.0	41.0	25.5	41.0
42-43	35.416633796946414	40.0	35.0	41.0	25.0	41.0
44-45	35.282077982797546	40.0	35.0	41.0	24.0	41.0
46-47	35.10959364842438	40.0	35.0	41.0	23.5	41.0
48-49	34.95355058445364	40.0	35.0	41.0	23.0	41.0
50-51	33.244504755607494	37.0	32.5	39.5	18.5	40.0
52-53	33.399781586846444	37.5	33.5	39.5	19.0	40.0
54-55	34.27841075857313	39.0	34.0	40.5	19.5	41.0
56-57	34.30699404908036	39.0	34.0	41.0	20.0	41.0
58-59	34.1440921246878	39.0	34.0	41.0	18.5	41.0
60-61	33.9174849999509	38.0	34.0	41.0	17.5	41.0
62-63	33.649259081044455	38.0	34.0	40.0	14.0	41.0
64-65	33.368500660667735	37.0	34.0	40.0	13.0	41.0
66-67	33.036130232832186	37.0	33.0	40.0	9.5	41.0
68-69	32.67819534597494	36.0	33.0	39.0	7.5	41.0
70-71	32.29402264819197	36.0	33.0	39.0	4.5	41.0
72-73	31.930177076411596	35.0	32.0	39.0	2.0	40.5
74-75	31.52189259757533	35.0	32.0	37.5	2.0	40.0
76-77	31.130841262982855	35.0	32.0	37.0	2.0	39.0
78-79	30.763704218344415	35.0	31.5	36.5	2.0	39.0
80-81	30.38689024623103	35.0	31.0	36.0	2.0	38.0
82-83	30.05991181640529	35.0	31.0	36.0	2.0	37.0
84-85	29.793271418237047	35.0	31.0	35.0	2.0	37.0
86-87	29.544894718695204	35.0	31.0	35.0	2.0	36.0
88-89	29.31647989573343	35.0	31.0	35.0	2.0	36.0
90-91	29.037544039969582	35.0	30.0	35.0	2.0	36.0
92-93	28.82911114275333	34.0	30.0	35.0	2.0	35.5
94-95	28.609314323996696	34.0	30.0	35.0	2.0	35.0
96-97	28.348289465213742	34.0	30.0	35.0	2.0	35.0
98-99	28.107272855230292	34.0	29.5	35.0	2.0	35.0
100-101	27.052655246007355	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	108495.0
3	19327.0
4	13286.0
5	9899.0
6	9425.0
7	9829.0
8	10546.0
9	11526.0
10	12784.0
11	13820.0
12	14396.0
13	15328.0
14	16782.0
15	18731.0
16	19971.0
17	21990.0
18	19330.0
19	16895.0
20	16612.0
21	16975.0
22	18745.0
23	21069.0
24	24111.0
25	28164.0
26	33362.0
27	39594.0
28	45972.0
29	54072.0
30	64073.0
31	78137.0
32	96179.0
33	124227.0
34	169972.0
35	246827.0
36	406600.0
37	744134.0
38	877983.0
39	196750.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6520910887198	18.748021353012454	12.88342084866216	24.71646670960558
2	15.671207736404153	20.06975777473845	39.607147838281406	24.651886650575996
3	18.83394548343483	23.99519231556488	30.104876077130367	27.065986123869923
4	12.804606447541806	15.866009736052325	37.658996896210276	33.6703869201956
5	13.577713898493068	37.37219253409268	35.28333446591251	13.766759101501746
6	32.7547977799295	36.25203906158559	17.254055546506702	13.739107611978211
7	27.954253412305853	32.40101380200305	21.66360652696424	17.981126258726853
8	25.93225171444279	35.33767054050478	20.39411380325959	18.335963941792844
9	26.15736616612968	15.759207485982335	20.12494826335392	37.958478084534065
10-11	24.81779078920264	25.792183413798327	28.72562006245302	20.664405734546015
12-13	25.471945097436798	23.353221196761606	28.416270221370432	22.758563484431164
14-15	22.56092410802674	25.05425611506158	25.81077841304565	26.574041363866037
16-17	22.555643385728562	28.11069112098799	26.72207243797309	22.61159305531036
18-19	22.515103899934328	27.36202130739216	28.233099497887242	21.889775294786272
20-21	22.986214871414834	26.620766261278415	28.28303361233562	22.109985254971125
22-23	22.79849392415776	26.526131245506384	27.968701153545588	22.706673676790274
24-25	22.539026208315978	26.676833781857116	28.104368798572548	22.67977121125436
26-27	22.511024088388623	26.870247629109844	27.960097854857906	22.65863042764363
28-29	22.57489499006684	27.113386718526726	27.857210773021258	22.454507518385178
30-31	22.821695850402346	26.791509617210362	27.779802657855164	22.606991874532127
32-33	22.505157031137788	26.868763088656195	27.720472885057983	22.905606995148034
34-35	22.950955850499067	26.896614939399328	27.709257002059122	22.443172208042476
36-37	22.942663050853746	26.749577767960663	27.66620625654932	22.64155292463627
38-39	22.696273199581533	26.932438017374366	27.739900074882456	22.631388708161644
40-41	22.917086361672915	26.733738759277674	27.780001754023925	22.569173125025486
42-43	22.77543341543955	26.737403488974298	27.56069360685488	22.926469488731275
44-45	22.756938020035502	26.854418072672765	27.605646890343138	22.7829970169486
46-47	22.95560402029251	26.88991178364095	27.276802143660394	22.877682052406143
48-49	22.69033124543232	27.046991165002847	27.27333228018141	22.98934530938342
50-51	22.82022872347985	27.064641549573697	27.306844431520155	22.808285295426295
52-53	23.010079360133638	26.94926483369794	27.24283391877694	22.79782188739148
54-55	22.64859982180047	26.884323361083627	27.479105285310563	22.987971531805336
56-57	22.75631822313603	27.017335641599686	27.308825565393825	22.917520569870454
58-59	22.720909077987976	26.934365505154485	27.412353590097098	22.932371826760445
60-61	22.650572638046587	26.88912059464645	27.574793784837016	22.88551298246995
62-63	22.74294553242679	26.834809379142342	27.573968912494934	22.848276175935933
64-65	22.518258156120645	26.722366280540566	27.519333069741464	23.240042493597326
66-67	22.494794744290616	26.881941138110232	27.391862145040918	23.23140197255823
68-69	22.696661949327137	26.69887084492853	27.435893651597965	23.168573554146366
70-71	22.555309418624812	26.563413947315095	27.452294213137517	23.42898242092257
72-73	22.559334468478408	26.619129221386224	27.41171755575509	23.409818754380286
74-75	22.53763370922178	26.744823187223925	27.30864219123934	23.40890091231495
76-77	22.565233390171393	26.523610932529046	27.401610695593725	23.509544981705833
78-79	22.492015032745574	26.544793380387315	27.406414024293653	23.556777562573455
80-81	22.604873065390414	26.56664950339741	27.276407867620755	23.552069563591417
82-83	22.558440433007725	26.55720643918425	27.34784820618468	23.53650492162335
84-85	22.55278226992463	26.572192171308796	27.305892785484588	23.569132773281986
86-87	22.427100825517158	26.61223823582002	27.287820436758015	23.67284050190481
88-89	22.413084149454555	26.409683602600253	27.301677827302633	23.875554420642562
90-91	22.374777523518944	26.3168967299033	27.393477850774666	23.91484789580309
92-93	22.420564235553375	26.19309831948741	27.55965457421227	23.82668287074694
94-95	22.3566522204511	26.0482917130202	27.627672913325302	23.967383153203397
96-97	22.336475788728777	25.875709814200743	27.589189741979958	24.198624655090523
98-99	22.2158334987475	25.869695741829226	27.51942181050044	24.39504894892284
100-101	22.322845561623428	25.670590853856613	27.666545078295986	24.340018506223977
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	158.0
1	119.0
2	155.5
3	241.0
4	230.0
5	265.0
6	438.5
7	786.5
8	1197.5
9	1336.5
10	1200.0
11	1102.0
12	1160.0
13	1291.0
14	1453.0
15	1658.5
16	1902.0
17	2157.5
18	2402.0
19	2713.5
20	3065.0
21	3477.5
22	4157.0
23	5095.0
24	6377.0
25	8374.5
26	11423.5
27	15712.0
28	21310.0
29	28003.0
30	35410.0
31	44018.5
32	54686.5
33	62198.5
34	67311.5
35	75086.0
36	84414.5
37	92997.5
38	100002.5
39	107029.5
40	114207.5
41	120091.0
42	124874.5
43	131117.0
44	136796.0
45	142635.5
46	146152.0
47	147475.0
48	150229.5
49	150561.5
50	147413.0
51	142827.0
52	138497.5
53	134347.5
54	129193.5
55	121778.0
56	111302.5
57	99064.5
58	88134.5
59	76062.5
60	61984.5
61	49878.0
62	40261.0
63	30397.5
64	21231.5
65	15173.0
66	11058.5
67	8213.5
68	6188.5
69	4517.5
70	3286.5
71	2354.5
72	1671.0
73	1235.0
74	946.0
75	697.0
76	488.5
77	345.0
78	243.0
79	177.5
80	138.5
81	114.0
82	90.0
83	68.0
84	56.5
85	50.0
86	42.0
87	34.5
88	36.5
89	38.5
90	30.5
91	25.0
92	22.5
93	21.0
94	18.0
95	15.5
96	14.5
97	11.0
98	6.0
99	4.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050954885831595735
2	0.05911094089778799
3	0.05057299696562018
4	0.05673777437351132
5	0.04869083041188351
6	0.019067165522636736
7	0.01576655460956228
8	0.0219040542413123
9	0.02157672092761897
10-11	0.02272238752554564
12-13	0.02789152610428621
14-15	0.045131080625468493
16-17	0.029650942665387887
18-19	0.02386805412347231
20-21	0.02793244276849788
22-23	0.04810435822484962
24-25	0.04253969189206293
26-27	0.0319831925254529
28-29	0.02017191545635174
30-31	0.012984221443168936
32-33	0.009860916075011703
34-35	0.009601777201671146
36-37	0.0014593610235494497
38-39	0.006628499602290025
40-41	0.003587027562556124
42-43	0.21608090370181243
44-45	0.19048071079337958
46-47	0.19638634932793017
48-49	0.19449054388612294
50-51	0.19290843286993847
52-53	0.19705465484338738
54-55	0.19552709937948515
56-57	0.2057426265443313
58-59	0.2025102100716096
60-61	0.20765207087420906
62-63	0.20481518215553352
64-65	0.20606995985802462
66-67	0.19601809935002515
68-69	0.19492698830438068
70-71	0.1787376281646312
72-73	0.18651179436484788
74-75	0.1864435999244951
76-77	0.18443868337812344
78-79	0.21024345960761465
80-81	0.21774484804642022
82-83	0.2167764869934108
84-85	0.2114573206458941
86-87	0.22918787513761638
88-89	0.2275239307930086
90-91	0.22635098641894083
92-93	0.23112459724363527
94-95	0.24166745772217477
96-97	0.2419265965955153
98-99	0.23191565275172749
100-101	0.24113554108742308
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3665988.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.87387030205943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5782363458864	46.44909261236115
2	12.636640333859784	15.132091288065874
3	4.17591035019919	7.500837441025617
4	1.8423958311367505	4.412454761541472
5	1.0268872163798286	3.074185600418434
6	0.6352946901153557	2.2822531127732306
7	0.41730551760180007	1.7489987506056768
8	0.2984440014772481	1.4295197949501106
9	0.22314852771481733	1.2024689405843239
>10	1.0895135735522046	11.391846843372088
>50	0.05510760901784188	2.2239351273154186
>100	0.019194101392045828	1.9272132049773054
>500	0.0011439891100035627	0.4473275047861439
>1k	7.779125566773764E-4	0.777775017223112
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4077	0.1112114933273104	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.183332842333363E-5
2	0.0	0.0	0.0	8.183332842333363E-5	8.183332842333363E-5
3	0.0	0.0	0.0	8.183332842333363E-5	8.183332842333363E-5
4	0.0	2.727777614111121E-5	0.0	8.183332842333363E-5	1.0911110456444484E-4
5	0.0	2.727777614111121E-5	0.0	8.183332842333363E-5	1.0911110456444484E-4
6	0.0	2.727777614111121E-5	0.0	8.183332842333363E-5	1.6366665684666726E-4
7	0.0	2.727777614111121E-5	0.0	8.183332842333363E-5	1.6366665684666726E-4
8	0.0	2.727777614111121E-5	0.0	8.183332842333363E-5	1.6366665684666726E-4
9	0.0	2.727777614111121E-5	0.0	8.183332842333363E-5	1.9094443298777846E-4
10-11	0.0	2.727777614111121E-5	0.0	1.9094443298777846E-4	1.9094443298777846E-4
12-13	0.0	2.727777614111121E-5	0.0	1.9094443298777846E-4	2.727777614111121E-4
14-15	0.0	2.727777614111121E-5	0.0	2.0458332105833407E-4	3.8188886597555693E-4
16-17	0.0	2.727777614111121E-5	0.0	2.454999852700009E-4	3.8188886597555693E-4
18-19	0.0	2.727777614111121E-5	0.0	2.454999852700009E-4	3.8188886597555693E-4
20-21	0.0	2.727777614111121E-5	0.0	3.000555375522233E-4	4.0916664211666814E-4
22-23	0.0	2.727777614111121E-5	0.0	3.8188886597555693E-4	4.3644441825777935E-4
24-25	0.0	2.727777614111121E-5	0.0	5.18277746681113E-4	4.909999705400018E-4
26-27	0.0	2.727777614111121E-5	0.0	0.001036555493362226	5.319166347516687E-4
28-29	0.0	2.727777614111121E-5	0.0	0.004337166406436682	5.455555228222242E-4
30-31	0.0	2.727777614111121E-5	0.0	0.016121165699396724	5.455555228222242E-4
32-33	0.0	2.727777614111121E-5	0.0	0.040275636472350704	5.455555228222242E-4
34-35	0.0	2.727777614111121E-5	0.0	0.07126319016865304	6.54666627386669E-4
36-37	0.0	2.727777614111121E-5	0.0	0.11954485393841988	6.819444035277803E-4
38-39	0.0	2.727777614111121E-5	0.0	0.1965227382086357	6.819444035277803E-4
40-41	0.0	2.727777614111121E-5	0.0	0.28390709407668546	6.955832915983358E-4
42-43	0.0	2.727777614111121E-5	0.0	0.37640603297119357	7.364999558100026E-4
44-45	0.0	2.727777614111121E-5	0.0	0.4940959981320179	8.046943961627806E-4
46-47	0.0	2.727777614111121E-5	0.0	0.6299393233147517	8.456110603744475E-4
48-49	0.0	2.727777614111121E-5	0.0	0.77153825926326	8.865277245861143E-4
50-51	0.0	2.727777614111121E-5	0.0	0.9213887224944544	9.274443887977811E-4
52-53	0.0	2.727777614111121E-5	0.0	1.0815092684427772	9.819999410800035E-4
54-55	0.0	2.727777614111121E-5	0.0	1.263574785296624	0.001036555493362226
56-57	0.0	2.727777614111121E-5	0.0	1.478169050198746	0.001036555493362226
58-59	0.0	2.727777614111121E-5	0.0	1.7084889530462184	0.001036555493362226
60-61	0.0	2.727777614111121E-5	0.0	1.9494608274767948	0.001063833269503337
62-63	0.0	2.727777614111121E-5	0.0	2.20364878444774	0.0011183888217855596
64-65	0.0	2.727777614111121E-5	0.0	2.482291267729191	0.0011183888217855596
66-67	0.0	2.727777614111121E-5	0.0	2.8067876927038498	0.0011183888217855596
68-69	0.0	2.727777614111121E-5	0.0	3.156584255049389	0.0011183888217855596
70-71	0.0	2.727777614111121E-5	0.0	3.519719649927932	0.001145666597926671
72-73	0.0	2.727777614111121E-5	0.0	3.8988398216251663	0.0012274999263500045
74-75	0.0	2.727777614111121E-5	0.0	4.30151435302025	0.0012274999263500045
76-77	0.0	2.727777614111121E-5	0.0	4.762249630931689	0.0012274999263500045
78-79	0.0	2.727777614111121E-5	0.0	5.275712850123895	0.0012547777024911157
80-81	0.0	2.727777614111121E-5	0.0	5.80041451308624	0.001309333254773338
82-83	0.0	2.727777614111121E-5	0.0	6.344537952661057	0.0014184443593377829
84-85	0.0	2.727777614111121E-5	0.0	6.918257779348977	0.0014320832474083384
86-87	1.3638888070555605E-5	2.727777614111121E-5	0.0	7.545060158407502	0.0015002776877611167
88-89	2.727777614111121E-5	2.727777614111121E-5	0.0	8.225163312045758	0.0015821110161844502
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3235	0.0	50.019127	1
GTATCAA	6145	0.0	44.01584	1
TCAACGC	7195	0.0	36.740047	4
CAACGCA	7415	0.0	35.71155	5
ATCAACG	7580	0.0	35.05892	3
TATCAAC	7785	0.0	34.197147	2
AACGCAG	7805	0.0	33.857536	6
ACGCAGA	9170	0.0	28.509974	7
CGCAGAG	9395	0.0	27.475542	8
GTACATG	7950	0.0	26.38221	1
TACATGG	8275	0.0	24.602259	2
ACATGGG	8320	0.0	23.727377	3
GCAGAGT	10855	0.0	22.556103	9
CATGGGG	6130	0.0	21.677683	4
GAGTACA	6150	0.0	20.67834	1
AGAGTAC	9135	0.0	19.479277	10-11
GAGTACT	6485	0.0	19.31759	12-13
CAGAGTA	10180	0.0	18.621689	10-11
GTATAGC	1260	0.0	17.700413	1
AGTACAT	6730	0.0	17.346254	2
>>END_MODULE