FastQCFastQC Report
Mon 15 Aug 2016
SRR2921389_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921389_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3665988
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41160.11227532659681375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32950.051.9281731
GTATCAA62900.043.52391
TCAACGC80250.033.709424
ATCAACG83600.032.528933
CAACGCA83550.032.2648055
TATCAAC87650.030.9175912
AACGCAG87900.030.7728966
GTACATG81450.025.733771
ACGCAGA105000.025.399117
CGCAGAG105750.025.0391628
TACATGG84900.023.6458052
ACATGGG87300.022.5064773
GCAGAGT120200.020.8425629
AGAGTAC104900.020.27974710-11
CATGGGG65750.018.9837254
GAGTACT72400.018.74211512-13
GTACCGT3951.8189894E-1118.0525746
CAGAGTA113000.017.98467610-11
AGTACTT82800.017.56269612-13
GTACTTT81850.016.34637814-15
Produced by FastQC (version 0.11.5)