##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921389_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3665988
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.489927408382133	33.0	31.0	34.0	30.0	34.0
2	31.617404366844628	34.0	31.0	34.0	29.0	34.0
3	31.69459092610232	34.0	31.0	34.0	29.0	34.0
4	35.201659143456006	37.0	35.0	37.0	33.0	37.0
5	34.95811606584637	37.0	35.0	37.0	32.0	37.0
6	34.919890899806546	37.0	35.0	37.0	32.0	37.0
7	34.785563673421734	37.0	35.0	37.0	32.0	37.0
8	34.75114212048703	37.0	35.0	37.0	32.0	37.0
9	36.38205007763255	39.0	37.0	39.0	32.0	39.0
10-11	36.23591730251163	39.0	37.0	39.0	32.0	39.0
12-13	36.179010678703804	39.0	37.0	39.0	32.0	39.0
14-15	37.426225754148675	40.0	38.0	41.0	32.0	41.0
16-17	37.41407405043333	40.0	38.0	41.0	32.0	41.0
18-19	37.39010629603807	40.0	38.0	41.0	32.0	41.0
20-21	37.36069635252488	40.0	38.0	41.0	32.0	41.0
22-23	37.254106941975806	40.0	38.0	41.0	31.0	41.0
24-25	37.1904721182939	40.0	38.0	41.0	31.0	41.0
26-27	37.05468321227457	40.0	37.0	41.0	31.0	41.0
28-29	36.89538795544339	40.0	37.0	41.0	30.0	41.0
30-31	36.74873458396482	40.0	37.0	41.0	30.0	41.0
32-33	36.67843184429409	40.0	37.0	41.0	30.0	41.0
34-35	36.787514579971344	40.0	37.0	41.0	30.0	41.0
36-37	36.74670307158671	40.0	37.0	41.0	30.0	41.0
38-39	36.65060169318612	40.0	37.0	41.0	30.0	41.0
40-41	36.51505433187452	40.0	37.0	41.0	30.0	41.0
42-43	36.396220064004574	40.0	37.0	41.0	30.0	41.0
44-45	36.24178256993749	40.0	36.0	41.0	29.0	41.0
46-47	36.06276220762316	40.0	36.0	41.0	28.0	41.0
48-49	35.88910492887592	40.0	36.0	41.0	27.5	41.0
50-51	35.735002133122094	40.0	35.5	41.0	27.0	41.0
52-53	35.568584512551595	39.5	35.0	41.0	27.0	41.0
54-55	35.36882553898158	39.0	35.0	41.0	26.0	41.0
56-57	35.116731833273874	39.0	35.0	41.0	26.0	41.0
58-59	34.910552216755754	39.0	35.0	41.0	26.0	41.0
60-61	34.6502187132091	38.0	35.0	40.0	25.5	41.0
62-63	34.36829266762466	38.0	34.0	40.0	24.5	41.0
64-65	34.06339928008494	37.0	34.0	40.0	23.5	41.0
66-67	33.73411696928632	37.0	34.0	40.0	23.0	41.0
68-69	33.377935225101666	36.0	34.0	39.0	22.0	41.0
70-71	32.9971781140582	36.0	33.5	39.0	22.0	40.5
72-73	32.59406863852255	35.0	33.0	38.5	20.5	40.0
74-75	32.17875290371927	35.0	33.0	37.0	19.5	39.5
76-77	29.37139646938288	33.0	29.0	34.5	12.0	37.0
78-79	31.030291970404704	34.5	31.5	36.0	14.5	39.0
80-81	31.217374279457545	35.0	32.0	36.0	17.0	37.5
82-83	31.034413915157387	35.0	32.0	35.5	13.5	37.0
84-85	30.779271099632624	35.0	32.0	35.0	9.5	37.0
86-87	30.51208991955238	35.0	32.0	35.0	7.0	36.0
88-89	30.205039678253172	35.0	32.0	35.0	2.0	36.0
90-91	30.00417295419407	34.5	31.0	35.0	2.0	36.0
92-93	29.847480542762277	34.0	31.0	35.0	2.0	35.0
94-95	29.740169498645386	34.0	31.0	35.0	2.0	35.0
96-97	29.536918560562665	34.0	31.0	35.0	2.0	35.0
98-99	29.26435452052762	34.0	31.0	35.0	2.0	35.0
100-101	28.173917235953855	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58372.0
3	20253.0
4	11290.0
5	8141.0
6	7198.0
7	7459.0
8	7769.0
9	8448.0
10	9702.0
11	10800.0
12	12062.0
13	13493.0
14	14886.0
15	17119.0
16	19367.0
17	21977.0
18	19512.0
19	16173.0
20	14743.0
21	14306.0
22	14955.0
23	16769.0
24	19110.0
25	22667.0
26	27344.0
27	32966.0
28	39102.0
29	46595.0
30	56868.0
31	70071.0
32	89450.0
33	116515.0
34	164234.0
35	244093.0
36	422939.0
37	832988.0
38	979618.0
39	156622.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.76240702195138	20.17013408618222	13.451810140238504	25.615648751627894
2	14.827422257295545	21.438494739865646	37.99809109237361	25.735991910465195
3	17.511655250113893	25.0093978552703	29.996426359824646	27.48252053479116
4	12.530668361598874	17.083498143667327	36.4972461898124	33.888587304921394
5	13.356427801002308	37.7379263601902	35.277754357702854	13.627891481104637
6	32.76184797149111	36.896417402993606	16.845592840479835	13.496141785035451
7	27.83206673249718	32.56880834047104	21.57638612321944	18.02273880381235
8	25.998330569134076	35.76160963687151	20.065413320530727	18.17464647346369
9	25.95868452343121	15.70657506423236	19.96193658078984	38.37280383154659
10-11	24.7530804360737	26.094259637242384	28.580849073991903	20.57181085269201
12-13	25.402573447678478	23.4970241220826	28.410761987868444	22.68964044237048
14-15	22.467839963161886	25.235308776317982	25.746074799868023	26.55077646065211
16-17	22.529246260517137	28.401507208915266	26.595824337902414	22.473422192665186
18-19	22.4677094726109	27.510870574795327	28.207015624358544	21.814404328235234
20-21	22.91283553109751	26.710080482318343	28.280719336719528	22.096364649864615
22-23	22.69950013297963	26.634701545973442	27.984260667353162	22.681537653693766
24-25	22.484822785536966	26.827638590325893	28.085065233618835	22.60247339051831
26-27	22.49682784518384	27.01976208463799	27.976789224207483	22.506620845970694
28-29	22.579980773406632	27.18911187760451	27.860064881568768	22.37084246742009
30-31	22.785472315351786	26.915245988001995	27.730699945409054	22.56858175123716
32-33	22.465403544267055	26.968573081461834	27.70375074973936	22.86227262453176
34-35	22.885935005905008	26.963991321316712	27.73441133936047	22.415662333417814
36-37	22.88245999947626	26.879361759237437	27.685700740217	22.552477501069298
38-39	22.681829694309446	27.034010155815153	27.74290683919723	22.541253310678172
40-41	22.874356758455434	26.869546904802434	27.721541881080512	22.534554455661617
42-43	22.759017005682747	26.88061103371101	27.49659883617043	22.86377312443581
44-45	22.74513500109393	26.940829981062148	27.535686479485083	22.778348538358838
46-47	22.938612596224722	27.036207612426537	27.219927335978078	22.805252455370663
48-49	22.66817984651773	27.199753380783886	27.2031771320695	22.92888964062888
50-51	22.800973491148053	27.216355720638102	27.19711013399484	22.785560654219005
52-53	23.092198906577934	27.0813727078754	27.058240737255186	22.76818764829148
54-55	22.625260504751825	27.039711289812217	27.31362317101114	23.021405034424816
56-57	22.68615707729498	27.18294949612885	27.14966861655339	22.98122481002278
58-59	22.666483342458623	27.05906231304409	27.284875725556024	22.989578618941266
60-61	22.634115322338758	27.09564419397954	27.37115649832714	22.89908398535457
62-63	22.704662190394227	27.024850310061055	27.32100400878339	22.949483490761327
64-65	22.43263144523967	26.923627391577043	27.296385348856344	23.347355814326942
66-67	22.404838165017445	27.087529718353576	27.208906808018586	23.29872530861039
68-69	22.588898449267745	26.91389287970662	27.179321860060156	23.31788681096548
70-71	22.494154985164887	26.77924844059029	27.17108104291302	23.555515531331803
72-73	22.54601185145895	26.871960805820628	27.060840363281756	23.521186979438664
74-75	22.40824211204121	27.0111566459668	26.943613781963833	23.63698746002816
76-77	22.44015869270201	26.742219515789838	27.119427770019723	23.698194021488426
78-79	22.40155884973006	26.7781465689433	27.107847599700886	23.712446981625757
80-81	22.516774182279082	26.78997391895772	26.95142474107278	23.741827157690416
82-83	22.400973469013305	26.7884594266273	26.993752800803765	23.81681430355563
84-85	22.34604859352834	26.781927789415494	26.96927725292096	23.90274636413521
86-87	22.250362406873037	26.869173166330423	26.894384672714516	23.986079754082027
88-89	22.188365260788327	26.663150295598076	26.909401114234697	24.239083329378897
90-91	22.120287319959804	26.571896762724588	26.980209535152035	24.32760638216357
92-93	22.11620652387502	26.494945525206877	27.132185978472084	24.256661972446018
94-95	21.991658005504952	26.33754252153015	27.17924376464555	24.49155570831935
96-97	21.91521712413253	26.16689634646125	27.18338459582653	24.734501933579686
98-99	21.777206573432697	26.192010586102306	27.069057486916382	24.96172535354862
100-101	21.72178152070597	26.153269745198738	27.094080078604765	25.03086865549053
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	141.0
1	111.5
2	134.5
3	201.5
4	191.0
5	197.0
6	336.5
7	699.5
8	1048.0
9	1111.0
10	924.5
11	796.5
12	846.5
13	937.5
14	1032.5
15	1160.0
16	1384.5
17	1593.5
18	1782.0
19	2108.0
20	2530.0
21	2996.5
22	3667.0
23	4695.5
24	6128.0
25	8192.0
26	11222.0
27	15673.5
28	21285.5
29	28038.5
30	35833.5
31	44396.0
32	55226.0
33	62645.0
34	67756.5
35	75047.5
36	84243.0
37	93481.5
38	100329.0
39	107162.0
40	114014.0
41	119932.5
42	124934.0
43	131155.0
44	137296.0
45	143626.0
46	147387.0
47	148976.5
48	151749.5
49	152014.0
50	149160.0
51	144392.5
52	139709.0
53	135630.5
54	130518.0
55	122940.5
56	111996.0
57	99132.0
58	87480.0
59	75315.0
60	61204.0
61	49150.0
62	39660.0
63	29757.0
64	20410.0
65	14084.5
66	10281.0
67	7716.5
68	5663.5
69	4068.0
70	2874.0
71	2024.5
72	1445.5
73	1036.5
74	746.5
75	501.0
76	325.5
77	195.5
78	101.0
79	53.0
80	32.0
81	22.0
82	18.5
83	15.0
84	7.5
85	3.5
86	4.0
87	3.5
88	3.0
89	2.5
90	3.0
91	2.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15223726864354167
2	4.6372219439889056E-4
3	0.007037666244406692
4	0.0043098886302955705
5	0.02839616496289677
6	0.0012002221502088932
7	2.182222091288897E-4
8	0.0018548887775955622
9	2.7277776141111213E-5
10-11	0.10264627161900147
12-13	3.000555375522233E-4
14-15	0.0059874718629739105
16-17	1.7730554491722286E-4
18-19	3.682499779050013E-4
20-21	7.501388438805582E-4
22-23	3.546110898344457E-4
24-25	0.0013229721428438936
26-27	0.0028505276067461215
28-29	0.0063284440647378
30-31	0.014866387996905608
32-33	0.014934582437258388
34-35	0.012411388144205601
36-37	9.819999410800035E-4
38-39	2.5913887334055646E-4
40-41	0.004909999705400018
42-43	0.00902894390270781
44-45	0.00856522170830892
46-47	0.009547221649388923
48-49	0.011402110426984486
50-51	0.005796527429986132
52-53	0.00180033322531334
54-55	7.637777319511139E-4
56-57	0.0059874718629739105
58-59	0.009574499425530035
60-61	0.007405916222311693
62-63	0.018044248917345063
64-65	0.03484735902026957
66-67	0.037520581082098464
68-69	0.026664026177936208
70-71	0.04202141414538182
72-73	0.026022998438620094
74-75	0.02476822073612898
76-77	0.24159926328182196
78-79	0.06867180143524747
80-81	0.05521021890960909
82-83	0.020335582113198407
84-85	0.032160498070370115
86-87	0.08123321734822918
88-89	0.2661356229207515
90-91	0.3219868695696767
92-93	0.05204599687724019
94-95	0.005400999675940019
96-97	0.010297360493269481
98-99	0.0013502499189850048
100-101	0.0014320832474083384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3665988.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.19101054373645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.15732075316456	45.67019786227019
2	12.789466847707839	15.140429340628852
3	4.210209776801864	7.476197138700642
4	1.9525009061022127	4.622820068790043
5	1.0679478395871496	3.1606455916581773
6	0.6457603883236777	2.2933925972396496
7	0.42845872409522695	1.7752633398833515
8	0.3003362085052596	1.4221762947440522
9	0.22052845791841355	1.1747972050058484
>10	1.15146199769778	11.883111809640212
>50	0.05453905144481583	2.19776108450779
>100	0.01957269454070036	1.9689128983573974
>500	0.0012025660322365062	0.4916149931654882
>1k	6.937880784790003E-4	0.7226797754083601
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4116	0.11227532659681375	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.183332842333363E-5
2	0.0	0.0	0.0	0.0	8.183332842333363E-5
3	0.0	0.0	0.0	0.0	8.183332842333363E-5
4	0.0	0.0	0.0	0.0	8.183332842333363E-5
5	0.0	0.0	0.0	0.0	8.183332842333363E-5
6	0.0	0.0	0.0	0.0	1.0911110456444484E-4
7	0.0	0.0	0.0	0.0	1.3638888070555605E-4
8	0.0	0.0	0.0	2.727777614111121E-5	1.3638888070555605E-4
9	0.0	0.0	0.0	2.727777614111121E-5	2.1822220912888967E-4
10-11	0.0	0.0	0.0	2.727777614111121E-5	2.454999852700009E-4
12-13	0.0	0.0	0.0	5.455555228222242E-5	3.546110898344457E-4
14-15	0.0	0.0	0.0	5.455555228222242E-5	3.9552775404611256E-4
16-17	0.0	0.0	0.0	5.455555228222242E-5	5.046388586105574E-4
18-19	0.0	0.0	0.0	5.455555228222242E-5	5.455555228222242E-4
20-21	0.0	0.0	0.0	8.183332842333363E-5	6.001110751044467E-4
22-23	0.0	0.0	0.0	1.5002776877611165E-4	6.54666627386669E-4
24-25	0.0	0.0	0.0	3.000555375522233E-4	7.364999558100027E-4
26-27	0.0	0.0	0.0	8.456110603744474E-4	7.637777319511139E-4
28-29	0.0	0.0	0.0	0.004228055301872237	7.637777319511139E-4
30-31	0.0	0.0	0.0	0.01628483235624339	7.637777319511139E-4
32-33	0.0	0.0	0.0	0.04090302532359626	7.910555080922251E-4
34-35	0.0	0.0	0.0	0.0723815789904386	8.456110603744475E-4
36-37	0.0	0.0	0.0	0.12135882605180376	8.456110603744475E-4
38-39	0.0	0.0	0.0	0.19971423801714572	8.456110603744475E-4
40-41	0.0	0.0	0.0	0.2882578993711927	8.456110603744475E-4
42-43	0.0	0.0	0.0	0.3814933382215108	8.456110603744475E-4
44-45	0.0	0.0	0.0	0.5000834699949918	8.865277245861143E-4
46-47	1.3638888070555605E-5	0.0	0.0	0.6368269617903823	9.274443887977812E-4
48-49	2.727777614111121E-5	0.0	0.0	0.7787123143883723	9.547221649388924E-4
50-51	2.727777614111121E-5	0.0	0.0	0.9292310831350239	0.0010092777172211148
52-53	2.727777614111121E-5	0.0	0.0	1.0898699068300277	0.0011183888217855594
54-55	2.727777614111121E-5	0.0	0.0	1.273490256923918	0.0012002221502088932
56-57	2.727777614111121E-5	0.0	0.0	1.4890119662148376	0.0012274999263500045
58-59	2.727777614111121E-5	0.0	0.0	1.7196864801521445	0.0012684165905616713
60-61	2.727777614111121E-5	0.0	0.0	1.9616812711880125	0.0013366110309144493
62-63	2.727777614111121E-5	0.0	0.0	2.2174649780632123	0.0014184443593377829
64-65	2.727777614111121E-5	0.0	0.0	2.498057822338753	0.0014184443593377829
66-67	2.727777614111121E-5	0.0	0.0	2.823468052814139	0.0014729999116200054
68-69	2.727777614111121E-5	0.0	0.0	3.1759105594453665	0.0015002776877611165
70-71	2.727777614111121E-5	0.0	0.0	3.54201923192329	0.0015411943519727835
72-73	2.727777614111121E-5	0.0	0.0	3.9242627089886817	0.0016639443446077838
74-75	2.727777614111121E-5	0.0	0.0	4.3323382400597055	0.0017321387849605616
76-77	2.727777614111121E-5	0.0	0.0	4.798215378773744	0.0017594165611016731
78-79	2.727777614111121E-5	0.0	0.0	5.319111791964403	0.00180033322531334
80-81	2.727777614111121E-5	0.0	0.0	5.849255371266899	0.001827611001454451
82-83	2.727777614111121E-5	0.0	0.0	6.400771088175957	0.0019094443298777848
84-85	2.727777614111121E-5	0.0	0.0	6.983697164311504	0.0019094443298777848
86-87	2.727777614111121E-5	0.0	0.0	7.620333181668897	0.0019094443298777848
88-89	2.727777614111121E-5	0.0	0.0	8.309656223642849	0.0019094443298777848
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3295	0.0	51.928173	1
GTATCAA	6290	0.0	43.5239	1
TCAACGC	8025	0.0	33.70942	4
ATCAACG	8360	0.0	32.52893	3
CAACGCA	8355	0.0	32.264805	5
TATCAAC	8765	0.0	30.917591	2
AACGCAG	8790	0.0	30.772896	6
GTACATG	8145	0.0	25.73377	1
ACGCAGA	10500	0.0	25.39911	7
CGCAGAG	10575	0.0	25.039162	8
TACATGG	8490	0.0	23.645805	2
ACATGGG	8730	0.0	22.506477	3
GCAGAGT	12020	0.0	20.842562	9
AGAGTAC	10490	0.0	20.279747	10-11
CATGGGG	6575	0.0	18.983725	4
GAGTACT	7240	0.0	18.742115	12-13
GTACCGT	395	1.8189894E-11	18.052574	6
CAGAGTA	11300	0.0	17.984676	10-11
AGTACTT	8280	0.0	17.562696	12-13
GTACTTT	8185	0.0	16.346378	14-15
>>END_MODULE