FastQCFastQC Report
Mon 15 Aug 2016
SRR2921388_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921388_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673253
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25470.15221846307760992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18470.1103837853570261No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17200.054.3510781
GTATCAA35550.045.9186631
TCAACGC42400.037.945144
CAACGCA42900.037.388915
ATCAACG43400.036.7383733
TATCAAC43650.036.6377682
AACGCAG45600.035.1666836
ACGCAGA53800.029.5439117
CGCAGAG54850.028.6383328
GTACATG50300.027.2646731
TACATGG51700.026.2517852
ACATGGG54800.024.6794053
GCAGAGT62450.024.0892459
CATGGGG46350.023.2433364
TATACCG1252.2709768E-422.7784735
GAGTACA40450.022.407081
ATGGGGG32650.021.365725
AGTACAT40400.021.1433852
GTGGTAT9600.020.2666991
CCGTATA1200.004789663519.7735739
Produced by FastQC (version 0.11.5)