##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921388_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1673253
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.305268240965354	31.0	31.0	34.0	26.0	34.0
2	30.358719213412435	31.0	31.0	34.0	26.0	34.0
3	30.413561487712855	31.0	31.0	34.0	26.0	34.0
4	33.8757288945545	37.0	35.0	37.0	30.0	37.0
5	33.8749800538233	37.0	35.0	37.0	30.0	37.0
6	33.92243014057049	37.0	35.0	37.0	30.0	37.0
7	33.834171520983375	37.0	35.0	37.0	29.0	37.0
8	33.80313975232676	37.0	35.0	37.0	29.0	37.0
9	35.344978912334234	39.0	35.0	39.0	29.0	39.0
10-11	35.23684583263858	39.0	35.0	39.0	29.0	39.0
12-13	35.112170723733946	39.0	35.0	39.0	28.0	39.0
14-15	36.21186784066725	40.0	36.0	41.0	27.0	41.0
16-17	36.16519797065955	40.0	36.0	41.0	27.0	41.0
18-19	36.13020834267144	40.0	36.0	41.0	27.0	41.0
20-21	36.07850815148696	40.0	36.0	41.0	27.0	41.0
22-23	35.96590473765772	40.0	36.0	41.0	27.0	41.0
24-25	35.86351585803223	40.0	36.0	41.0	27.0	41.0
26-27	35.71453450255281	40.0	36.0	41.0	27.0	41.0
28-29	35.561652063375945	40.0	36.0	41.0	26.0	41.0
30-31	35.39472124060139	39.0	35.0	41.0	25.0	41.0
32-33	35.19020644218179	39.0	35.0	41.0	25.0	41.0
34-35	35.0175003421479	39.0	35.0	41.0	24.0	41.0
36-37	34.83959747868374	39.0	35.0	41.0	24.0	41.0
38-39	35.046213872020544	39.0	35.0	41.0	24.0	41.0
40-41	35.14299600837411	40.0	35.0	41.0	24.0	41.0
42-43	34.96935848912268	40.0	35.0	41.0	23.0	41.0
44-45	34.830363967672554	40.0	35.0	41.0	22.0	41.0
46-47	34.63740330960112	39.5	35.0	41.0	19.5	41.0
48-49	34.473386570948925	39.0	35.0	41.0	17.5	41.0
50-51	32.77181275037307	37.0	32.0	39.5	13.5	40.0
52-53	32.92537201487163	37.5	32.5	39.5	13.0	40.0
54-55	33.78338362459233	39.0	34.0	40.5	12.0	41.0
56-57	33.81512269812156	39.0	34.0	41.0	10.5	41.0
58-59	33.648998985807886	38.0	34.0	41.0	8.0	41.0
60-61	33.41763409358896	38.0	33.5	40.0	7.5	41.0
62-63	33.13731037685275	37.5	33.0	40.0	4.0	41.0
64-65	32.86467079395644	37.0	33.0	40.0	2.0	41.0
66-67	32.539902513248144	36.5	33.0	39.5	2.0	41.0
68-69	32.178171800678086	36.0	32.0	39.0	2.0	41.0
70-71	31.801642070864357	35.5	32.0	39.0	2.0	41.0
72-73	31.444876238082344	35.0	32.0	38.5	2.0	40.0
74-75	31.048460991852394	35.0	31.5	37.0	2.0	39.5
76-77	30.665373676305975	35.0	31.0	37.0	2.0	39.0
78-79	30.30810283920005	35.0	31.0	36.0	2.0	39.0
80-81	29.947518695618655	35.0	31.0	36.0	2.0	37.5
82-83	29.631613987842844	35.0	31.0	35.5	2.0	37.0
84-85	29.37898363248116	35.0	30.0	35.0	2.0	37.0
86-87	29.1417950543044	35.0	30.0	35.0	2.0	36.0
88-89	28.92704749371434	35.0	30.0	35.0	2.0	36.0
90-91	28.662369946445637	34.0	30.0	35.0	2.0	36.0
92-93	28.44693181485406	34.0	29.5	35.0	2.0	35.0
94-95	28.229059203838272	34.0	29.0	35.0	2.0	35.0
96-97	27.973927134748898	34.0	29.0	35.0	2.0	35.0
98-99	27.74267489734069	34.0	29.0	35.0	2.0	35.0
100-101	26.71353973368044	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58579.0
3	9989.0
4	6996.0
5	5174.0
6	4817.0
7	4903.0
8	5375.0
9	5549.0
10	6115.0
11	6758.0
12	6922.0
13	7333.0
14	8061.0
15	8842.0
16	9510.0
17	10520.0
18	9538.0
19	8091.0
20	7845.0
21	8144.0
22	8714.0
23	10019.0
24	11544.0
25	13445.0
26	15798.0
27	18390.0
28	21924.0
29	25592.0
30	30275.0
31	36533.0
32	45020.0
33	59029.0
34	82064.0
35	116304.0
36	185130.0
37	334994.0
38	378739.0
39	80625.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.0168576446297	18.738762118375444	12.77526269809616	24.46911753889869
2	15.64952395532398	19.637523120504095	39.14109233430828	25.571860589863636
3	19.2774571140021	24.14077023485424	29.672643908540962	26.909128742602697
4	13.293032389855117	15.280830879556431	37.04890172055408	34.377235010034376
5	13.586261082954435	38.11621465832092	34.530922250099856	13.766602008624792
6	33.464680126126396	35.444386329333355	17.050017185468565	14.040916359071687
7	28.555375105873885	32.51023173604482	20.89587389217212	18.038519265909176
8	26.792470199471207	35.06345072220067	19.888598835655785	18.255480242672338
9	27.30946309294521	15.20074984547588	19.237804550003048	38.25198251157586
10-11	25.631174517075166	25.47389834196479	27.950923377331012	20.94400376362903
12-13	25.996177538224618	22.790068294969224	28.07393121720957	23.139822949596592
14-15	23.014994161263918	24.523319367802106	25.52529192185601	26.936394549077967
16-17	23.040881239143907	27.730744041720882	26.275373482538033	22.953001236597178
18-19	23.01320836433099	27.097904971869717	27.86064908121899	22.0282375825803
20-21	23.36019268430907	26.182068498817546	28.15299966283833	22.30473915403506
22-23	23.111042149312063	25.95458028424021	27.825607568896277	23.108769997551455
24-25	22.935253493349325	26.05552348446143	27.935464255744176	23.07375876644507
26-27	22.705159786682067	26.511158957490956	27.820437976713897	22.963243279113083
28-29	22.811166719219568	27.022880685987204	27.56149062939446	22.604461965398773
30-31	23.424594078461727	26.414143074454067	27.507897277120456	22.65336556996375
32-33	22.96345794138364	26.41991704617687	27.48796470263723	23.128660309802257
34-35	23.225499893909827	26.683471748635025	27.65525773935288	22.43577061810227
36-37	23.098656701119957	26.701864027780974	27.67005043832847	22.529428832770602
38-39	23.076127708889047	26.81859176917298	27.4989854460063	22.60629507593167
40-41	23.256479714798417	26.523956305348577	27.444855001113133	22.77470897873987
42-43	22.837770285266213	26.58502112023664	27.330960981998903	23.246247612498244
44-45	22.731649884478983	26.72658708152825	27.573202237837958	22.968560796154808
46-47	22.88594235625522	26.63938267549409	27.204446969150244	23.27022799910045
48-49	22.74153964455368	26.89874403217654	27.21327497862004	23.14644134464974
50-51	23.06338634417994	26.83212606422686	27.229116031082473	22.875371560510725
52-53	23.16101816979658	26.70328554791922	27.198757397566002	22.9369388847182
54-55	22.42820781554125	26.554783650247042	27.72783350801018	23.289175026201526
56-57	22.803442137493832	26.68818551652427	27.24285793006953	23.26551441591237
58-59	22.860446423865493	26.775878880514416	27.386734977456	22.97693971816409
60-61	23.11188937895005	26.816907499433217	27.37904528818022	22.692157833436507
62-63	23.001707632476283	26.686384401187617	27.44864973648793	22.863258229848167
64-65	22.585407898070557	26.478380063574132	27.49662034072716	23.43959169762816
66-67	22.64892218217273	26.729738381346312	27.327136957591293	23.294202478889673
68-69	22.741326303124456	26.637037418554254	27.380323899466173	23.241312378855113
70-71	22.664062747934636	26.4786859291984	27.36276284439899	23.49448847846797
72-73	22.59004390326132	26.840692811009383	27.18798458490414	23.38127870082516
74-75	22.568911942540996	26.82138855670418	27.213233440384304	23.39646606037052
76-77	22.487033321586498	26.407711352441847	27.20300295786679	23.90225236810486
78-79	22.4506769333519	26.457948669568772	27.37625993761521	23.715114459464115
80-81	22.73511062628775	26.708305964675187	26.965593815267326	23.59098959376974
82-83	22.747460237493776	26.717181368305848	27.13485915767164	23.40049923652873
84-85	22.652153117069705	26.720790977271562	27.056474715748536	23.5705811899102
86-87	22.39024201740383	26.97617390767745	26.99169110825464	23.64189296666408
88-89	22.31234509247571	26.608866476828503	27.173408357951782	23.905380072744002
90-91	22.576421490039326	26.375718623214972	27.22395762189935	23.823902264846357
92-93	22.800606912996653	26.179009377794355	27.267738200619796	23.752645508589197
94-95	22.61889389780004	26.226807904179633	27.248699678743275	23.905598519277053
96-97	22.379239380894074	26.04089363562199	27.362160669019325	24.21770631446461
98-99	22.121777214590736	25.98580948400983	27.38239351496115	24.510019786438274
100-101	22.364061136836455	26.00355800665344	27.422146883718256	24.210233972791855
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	79.0
1	78.5
2	115.5
3	173.5
4	168.5
5	178.0
6	275.0
7	498.0
8	767.5
9	870.5
10	786.5
11	713.5
12	764.0
13	849.0
14	929.5
15	1036.0
16	1160.0
17	1275.0
18	1417.0
19	1608.0
20	1842.0
21	2061.5
22	2387.5
23	2875.5
24	3523.0
25	4371.5
26	5654.5
27	7428.5
28	9444.5
29	11997.5
30	15121.5
31	18686.0
32	22877.5
33	25993.0
34	28353.5
35	31264.0
36	35439.0
37	39837.5
38	43365.5
39	46529.5
40	48719.0
41	50707.5
42	52858.0
43	55737.5
44	58750.0
45	61626.0
46	64138.0
47	66300.0
48	68391.5
49	69649.0
50	69581.5
51	68796.5
52	67525.0
53	65539.5
54	63197.5
55	59423.0
56	54320.0
57	48224.5
58	41915.0
59	35672.5
60	29291.0
61	23775.0
62	19336.0
63	14907.5
64	10763.5
65	7865.5
66	5756.5
67	4302.0
68	3318.0
69	2594.5
70	1961.5
71	1394.0
72	1030.0
73	764.5
74	591.0
75	457.0
76	328.5
77	238.0
78	160.0
79	108.0
80	75.5
81	62.0
82	55.5
83	46.5
84	36.0
85	30.0
86	25.5
87	23.5
88	22.5
89	17.5
90	13.0
91	12.0
92	15.0
93	11.5
94	7.0
95	9.0
96	6.0
97	4.5
98	5.5
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.053548387482347255
2	0.06191532302646401
3	0.051038306819112235
4	0.05982358914043483
5	0.051695708897578546
6	0.019602534703359265
7	0.01625576048571256
8	0.02271025361974549
9	0.02241143449316989
10-11	0.023875648213390326
12-13	0.030419787085395934
14-15	0.04793058790272601
16-17	0.03092777960057445
18-19	0.024592814117171763
20-21	0.027790178771530664
22-23	0.0495740930988918
24-25	0.0450320423749427
26-27	0.032182819932191964
28-29	0.020050763393222662
30-31	0.0124308756655449
32-33	0.009173747185870875
34-35	0.008845046146637718
36-37	0.0012849222442750737
38-39	0.00621543783277245
40-41	0.0030180731784135452
42-43	0.2320330517859523
44-45	0.20481062935491526
46-47	0.21105594910034525
48-49	0.2070816547168898
50-51	0.20543814952072398
52-53	0.21105594910034525
54-55	0.20935268007886434
56-57	0.22166402809377903
58-59	0.21688292206856943
60-61	0.22312824181399943
62-63	0.22052851541279175
64-65	0.22148473661783366
66-67	0.20998020024467312
68-69	0.20956185346746725
70-71	0.1916028239602738
72-73	0.19910318403732133
74-75	0.1991330659499789
76-77	0.1971907416272375
78-79	0.22563832247723448
80-81	0.23406502184666633
82-83	0.23331797403022733
84-85	0.2285667499176753
86-87	0.2472728272413078
88-89	0.24574884969577226
90-91	0.2443742817135245
92-93	0.24804975697040435
94-95	0.25928535612964687
96-97	0.26071968793720973
98-99	0.24984267172985797
100-101	0.25943476569293467
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1673253.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.48133185338511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.13280512775229	55.42431078875264
2	10.5639371545815	14.257370976132384
3	3.174555154436313	6.42669629590173
4	1.3897535611604042	3.7512968502035595
5	0.7430251317688984	2.5070162746151117
6	0.4643957166390811	1.8802824879487459
7	0.315787605262833	1.4916837730148913
8	0.22780453107134166	1.229804252714399
9	0.1654380920552334	1.0047584512053076
>10	0.7850575586030979	8.903175936137144
>50	0.026152776253756147	1.1774485050197065
>100	0.010310103845700198	1.2978079743681896
>500	7.108993232611895E-4	0.30450501940271035
>1k	2.665872462229461E-4	0.34384241458352527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2547	0.15221846307760992	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1847	0.1103837853570261	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.976382531511971E-5	0.0
5	0.0	0.0	0.0	5.976382531511971E-5	5.976382531511971E-5
6	0.0	0.0	0.0	5.976382531511971E-5	5.976382531511971E-5
7	0.0	0.0	0.0	5.976382531511971E-5	5.976382531511971E-5
8	0.0	0.0	0.0	5.976382531511971E-5	5.976382531511971E-5
9	0.0	0.0	0.0	5.976382531511971E-5	5.976382531511971E-5
10-11	0.0	0.0	0.0	5.976382531511971E-5	5.976382531511971E-5
12-13	0.0	0.0	0.0	5.976382531511971E-5	5.976382531511971E-5
14-15	0.0	0.0	0.0	5.976382531511971E-5	5.976382531511971E-5
16-17	0.0	0.0	0.0	8.964573797267956E-5	1.1952765063023942E-4
18-19	0.0	0.0	0.0	1.1952765063023942E-4	1.1952765063023942E-4
20-21	0.0	0.0	0.0	1.1952765063023942E-4	1.1952765063023942E-4
22-23	0.0	0.0	0.0	1.7929147594535912E-4	1.4940956328779928E-4
24-25	0.0	0.0	0.0	1.7929147594535912E-4	1.7929147594535912E-4
26-27	0.0	2.9881912657559855E-5	0.0	5.677563404936373E-4	1.7929147594535912E-4
28-29	0.0	5.976382531511971E-5	0.0	0.0029583093530984256	1.7929147594535912E-4
30-31	0.0	5.976382531511971E-5	0.0	0.012640049054147819	1.7929147594535912E-4
32-33	0.0	5.976382531511971E-5	0.0	0.03254140288408268	1.7929147594535912E-4
34-35	0.0	5.976382531511971E-5	0.0	0.05937536045057143	1.7929147594535912E-4
36-37	0.0	5.976382531511971E-5	0.0	0.10001476166485285	1.7929147594535912E-4
38-39	0.0	5.976382531511971E-5	0.0	0.16656178115323864	1.7929147594535912E-4
40-41	0.0	5.976382531511971E-5	0.0	0.24371687963505817	1.7929147594535912E-4
42-43	0.0	5.976382531511971E-5	0.0	0.32374064173200345	1.7929147594535912E-4
44-45	0.0	5.976382531511971E-5	0.0	0.4171813826121932	1.7929147594535912E-4
46-47	0.0	5.976382531511971E-5	0.0	0.5236207554984214	2.3905530126047884E-4
48-49	0.0	5.976382531511971E-5	0.0	0.6391442298325478	3.5858295189071824E-4
50-51	0.0	5.976382531511971E-5	0.0	0.7614210164272827	3.5858295189071824E-4
52-53	0.0	5.976382531511971E-5	0.0	0.8962780882508503	4.18346777205838E-4
54-55	2.9881912657559855E-5	5.976382531511971E-5	0.0	1.0511261596423256	4.781106025209577E-4
56-57	5.976382531511971E-5	5.976382531511971E-5	0.0	1.2188832173018667	5.079925151785175E-4
58-59	5.976382531511971E-5	8.964573797267956E-5	0.0	1.395754258321963	5.378744278360774E-4
60-61	5.976382531511971E-5	1.1952765063023942E-4	0.0	1.5876857833214701	5.378744278360774E-4
62-63	5.976382531511971E-5	1.1952765063023942E-4	0.0	1.7903150330523836	5.378744278360774E-4
64-65	5.976382531511971E-5	1.1952765063023942E-4	0.0	2.0151166619752066	5.378744278360774E-4
66-67	5.976382531511971E-5	1.1952765063023942E-4	0.0	2.2738043798517023	5.677563404936372E-4
68-69	5.976382531511971E-5	1.1952765063023942E-4	0.0	2.546656124327881	5.976382531511971E-4
70-71	5.976382531511971E-5	1.1952765063023942E-4	0.0	2.832566264635414	5.976382531511971E-4
72-73	5.976382531511971E-5	1.1952765063023942E-4	0.0	3.1369434269653187	7.171659037814365E-4
74-75	5.976382531511971E-5	1.1952765063023942E-4	0.0	3.452586070367123	7.171659037814365E-4
76-77	5.976382531511971E-5	1.1952765063023942E-4	0.0	3.8083003586427155	7.171659037814365E-4
78-79	5.976382531511971E-5	1.1952765063023942E-4	0.0	4.210152320061581	7.171659037814365E-4
80-81	5.976382531511971E-5	1.1952765063023942E-4	0.0	4.620430980849878	7.171659037814365E-4
82-83	5.976382531511971E-5	1.1952765063023942E-4	0.0	5.054286470724989	7.769297290965562E-4
84-85	5.976382531511971E-5	1.1952765063023942E-4	0.0	5.512854302367902	7.769297290965562E-4
86-87	5.976382531511971E-5	1.1952765063023942E-4	0.0	5.992548646259711	7.769297290965562E-4
88-89	5.976382531511971E-5	1.1952765063023942E-4	0.0	6.5155717635049815	7.769297290965562E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1720	0.0	54.351078	1
GTATCAA	3555	0.0	45.918663	1
TCAACGC	4240	0.0	37.94514	4
CAACGCA	4290	0.0	37.38891	5
ATCAACG	4340	0.0	36.738373	3
TATCAAC	4365	0.0	36.637768	2
AACGCAG	4560	0.0	35.166683	6
ACGCAGA	5380	0.0	29.543911	7
CGCAGAG	5485	0.0	28.638332	8
GTACATG	5030	0.0	27.264673	1
TACATGG	5170	0.0	26.251785	2
ACATGGG	5480	0.0	24.679405	3
GCAGAGT	6245	0.0	24.089245	9
CATGGGG	4635	0.0	23.243336	4
TATACCG	125	2.2709768E-4	22.778473	5
GAGTACA	4045	0.0	22.40708	1
ATGGGGG	3265	0.0	21.36572	5
AGTACAT	4040	0.0	21.143385	2
GTGGTAT	960	0.0	20.266699	1
CCGTATA	120	0.0047896635	19.773573	9
>>END_MODULE