FastQCFastQC Report
Mon 15 Aug 2016
SRR2921388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673253
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24530.14660066349798864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19890.11887024855177311No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.059.875641
GTATCAA35950.044.6764831
TCAACGC47350.033.2752084
CAACGCA47950.032.8118365
ATCAACG49400.031.8943523
AACGCAG49850.031.7500386
TATCAAC49800.031.7334672
GTACATG52600.027.2823891
ACGCAGA58550.026.7067587
TACATGG54900.025.67362
CGCAGAG61000.025.634118
ACATGGG55900.024.450253
CATGGGG47500.022.4797534
GCAGAGT67350.022.4409739
ATGGGGG33900.022.4342445
AGAGTAC59250.021.33759510-11
GAGTACA40550.020.6244811
GAGTACT37600.019.68500712-13
CAGAGTA64300.019.36611410-11
AGTACAT42000.019.3218822
Produced by FastQC (version 0.11.5)