##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921388_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1673253
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.552709004555798	33.0	31.0	34.0	30.0	34.0
2	31.682599104857424	34.0	31.0	34.0	30.0	34.0
3	31.75531554403309	34.0	31.0	34.0	30.0	34.0
4	35.25746793820181	37.0	35.0	37.0	33.0	37.0
5	35.02623512403683	37.0	35.0	37.0	32.0	37.0
6	34.99319110738185	37.0	35.0	37.0	32.0	37.0
7	34.873993950705604	37.0	35.0	37.0	32.0	37.0
8	34.834552216550634	37.0	35.0	37.0	32.0	37.0
9	36.47171408029748	39.0	37.0	39.0	32.0	39.0
10-11	36.33040520471202	39.0	37.0	39.0	32.0	39.0
12-13	36.26223290799419	39.0	37.0	39.0	32.0	39.0
14-15	37.5065384613086	40.0	38.0	41.0	32.0	41.0
16-17	37.4970596197945	40.0	38.0	41.0	32.0	41.0
18-19	37.48095267123382	40.0	38.0	41.0	32.0	41.0
20-21	37.45522852790343	40.0	38.0	41.0	32.0	41.0
22-23	37.351593572520116	40.0	38.0	41.0	32.0	41.0
24-25	37.28499665023759	40.0	38.0	41.0	31.0	41.0
26-27	37.149479486963415	40.0	37.0	41.0	31.0	41.0
28-29	36.99195011154918	40.0	37.0	41.0	30.5	41.0
30-31	36.83566442133975	40.0	37.0	41.0	30.0	41.0
32-33	36.75959821975517	40.0	37.0	41.0	30.0	41.0
34-35	36.86478195467153	40.0	37.0	41.0	30.0	41.0
36-37	36.82798955089278	40.0	37.0	41.0	30.0	41.0
38-39	36.730301394947446	40.0	37.0	41.0	30.0	41.0
40-41	36.60094916907366	40.0	37.0	41.0	30.0	41.0
42-43	36.48065534620287	40.0	36.5	41.0	30.0	41.0
44-45	36.3221001247271	40.0	36.0	41.0	29.5	41.0
46-47	36.121318473655805	40.0	36.0	41.0	28.5	41.0
48-49	35.94291538697375	40.0	36.0	41.0	28.0	41.0
50-51	35.788481180072594	40.0	35.0	41.0	27.5	41.0
52-53	35.62373801212369	39.5	35.0	41.0	27.0	41.0
54-55	35.42117510023888	39.0	35.0	41.0	27.0	41.0
56-57	35.173604051509244	39.0	35.0	41.0	26.0	41.0
58-59	34.95767107544406	39.0	35.0	41.0	26.0	41.0
60-61	34.68903297947172	38.0	35.0	40.0	26.0	41.0
62-63	34.39782776424127	38.0	34.0	40.0	25.0	41.0
64-65	34.099761064226385	37.0	34.0	40.0	24.5	41.0
66-67	33.778632400479786	37.0	34.0	39.5	23.5	41.0
68-69	33.41856670808299	36.0	34.0	39.0	23.0	41.0
70-71	33.04080405055302	36.0	33.5	39.0	22.0	40.5
72-73	32.641594247851344	35.0	33.0	38.5	21.5	40.0
74-75	32.227544489685656	35.0	33.0	37.0	20.0	39.5
76-77	29.453005313601707	33.0	29.0	34.5	13.0	37.0
78-79	31.10503701472521	34.5	31.5	36.0	17.0	38.5
80-81	31.28884155593924	35.0	32.0	36.0	18.0	37.5
82-83	31.107841133408996	35.0	32.0	35.5	17.5	37.0
84-85	30.859204495673996	35.0	32.0	35.0	13.5	37.0
86-87	30.599736262238885	35.0	32.0	35.0	7.5	36.0
88-89	30.309021110376015	35.0	32.0	35.0	2.0	36.0
90-91	30.110357190454764	35.0	32.0	35.0	2.0	36.0
92-93	29.954182063322165	34.0	31.0	35.0	2.0	35.0
94-95	29.843771384243745	34.0	31.0	35.0	2.0	35.0
96-97	29.654268661105043	34.0	31.0	35.0	2.0	35.0
98-99	29.395540005008208	34.0	31.0	35.0	2.0	35.0
100-101	28.328448238252076	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25516.0
3	8719.0
4	4938.0
5	3508.0
6	3114.0
7	3355.0
8	3352.0
9	3675.0
10	4235.0
11	4611.0
12	5163.0
13	5903.0
14	6580.0
15	7585.0
16	8916.0
17	10370.0
18	9060.0
19	7273.0
20	6754.0
21	6546.0
22	6620.0
23	7360.0
24	8590.0
25	10241.0
26	12205.0
27	14482.0
28	17565.0
29	21257.0
30	25604.0
31	32011.0
32	40367.0
33	53480.0
34	78324.0
35	114202.0
36	191604.0
37	380452.0
38	447698.0
39	72015.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.12225895603734	20.386369499879383	13.386871721566859	25.104499822516413
2	15.562749852232704	21.521372619213395	38.04274225021052	24.87313527834338
3	18.261629915452485	25.042763853874927	29.692279555471085	27.003326675201507
4	12.855626491924928	16.48573310367003	36.25881109794917	34.39982930645588
5	13.398211244375391	38.383281035256815	34.68584986815385	13.532657852213944
6	33.227828620982095	36.242781164779196	16.791445512174977	13.737944702063738
7	28.45771495131334	32.77960090865029	20.820486585694557	17.942197554341817
8	26.933579567357256	35.762981822311076	19.5498459543212	17.75359265601047
9	27.14634308141088	15.387153048582611	19.130938357797657	38.33556551220885
10-11	25.68699370495715	25.902831438059653	27.801036798912527	20.60913805807067
12-13	26.09419057329287	23.005314221578896	28.12105930628252	22.77943589884572
14-15	23.000611421935524	24.85197074498446	25.43718461416168	26.71023321891834
16-17	23.039480962044216	28.072502038697756	26.242739053560154	22.645277945697877
18-19	23.033136749603166	27.31657231922584	27.93079686932242	21.71949406184857
20-21	23.31734633808162	26.39841385101316	28.228700690578428	22.05553912032679
22-23	23.193655933353593	26.068781773896703	27.900607710092075	22.836954582657626
24-25	22.996617612738024	26.265631102543495	27.947262370037766	22.790488914680708
26-27	22.76479322785323	26.69706339295239	27.897991072829324	22.640152306365056
28-29	22.786050115754954	27.200815947677025	27.593817995017172	22.419315941550842
30-31	23.45631304731412	26.596899759981994	27.559990304838706	22.38679688786518
32-33	23.10394511395292	26.59520195386294	27.493173165347024	22.80767976683711
34-35	23.245831363353535	26.792721465079204	27.7508339664166	22.210613205150658
36-37	23.196677100531158	26.790010384060842	27.79402206799692	22.219290447411083
38-39	23.206477452120886	26.89666012351408	27.606326727528245	22.290535696836784
40-41	23.347297789563743	26.64387170152103	27.542222831841112	22.466607677074116
42-43	22.95548362668538	26.67196022242497	27.389668361819812	22.982887789069835
44-45	22.782424658815597	26.819767193401734	27.62500631311056	22.77280183467211
46-47	22.99077871669478	26.7330549654949	27.272298016957887	23.00386830085244
48-49	22.830561007164444	27.052601724929897	27.286425688120552	22.830411579785107
50-51	23.123548403677358	27.016844792900613	27.18957205476112	22.670034748660918
52-53	23.309549999238	26.7617940265851	27.200167580019606	22.72848839415729
54-55	22.54490992913142	26.687357088471575	27.697434555036406	23.0702984273606
56-57	22.832072141639166	26.877249121867514	27.175189597125904	23.11548913936741
58-59	22.934110656132088	26.915419525393986	27.310974345120087	22.839495473353843
60-61	23.16556463176215	26.981496213355072	27.343276053019604	22.50966310186317
62-63	22.99440563369842	26.87166790301823	27.37533421840564	22.75859224487771
64-65	22.61773055645151	26.633559392661976	27.466044019541524	23.28266603134499
66-67	22.723599898959854	26.952219669646553	27.11268816111419	23.2114922702794
68-69	22.74415829675201	26.874674564207474	27.19411725672049	23.187049882320025
70-71	22.72557686800122	26.683579239269058	27.18976061913648	23.401083273593247
72-73	22.660430404884753	27.124480734960667	26.92033163617582	23.294757223978763
74-75	22.625640798785522	27.06896072457526	26.88914401447381	23.416254462165412
76-77	22.507120629515175	26.653052494347236	27.06731915638065	23.77250771975695
78-79	22.47622129128548	26.759663319740092	27.152209752029194	23.611905636945238
80-81	22.749244378773064	26.997586257724766	26.749963149543486	23.503206213958684
82-83	22.773465368669882	27.05005811814377	26.846667031306087	23.32980948188026
84-85	22.62366558228335	27.128185706048324	26.69158267254503	23.556566039123293
86-87	22.440370056458313	27.35781794212769	26.592918157278184	23.608893844135817
88-89	22.31759695229649	27.05316253170322	26.711539297008773	23.917701218991517
90-91	22.55441511434024	26.75530451059523	26.85384318830031	23.83643718676422
92-93	22.688293244435144	26.665301302079442	26.77867639364358	23.867729059841835
94-95	22.528061498169176	26.721246172277475	26.773811519610703	23.97688080994265
96-97	22.150182031399893	26.645051620889348	26.844715351424302	24.360050996286454
98-99	21.899889344995888	26.63133954112987	26.836752044039674	24.632019069834573
100-101	22.039046681026136	26.7685276603069	26.78648695814318	24.405938700523777
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	82.0
1	77.5
2	104.5
3	148.0
4	132.0
5	125.5
6	221.5
7	484.0
8	742.5
9	815.0
10	680.5
11	535.5
12	553.5
13	616.0
14	690.5
15	778.0
16	879.0
17	1032.0
18	1193.5
19	1365.5
20	1551.5
21	1822.0
22	2195.5
23	2700.0
24	3343.0
25	4156.5
26	5345.5
27	7185.0
28	9388.5
29	12018.5
30	15206.5
31	18867.0
32	23733.5
33	27021.5
34	29034.5
35	32233.0
36	36254.0
37	40265.5
38	43669.5
39	46847.5
40	49571.5
41	52153.0
42	54219.0
43	56681.5
44	59827.5
45	62936.5
46	65430.0
47	67559.5
48	69682.0
49	70719.0
50	70667.5
51	69634.0
52	67634.0
53	65892.5
54	63431.5
55	58952.0
56	53324.5
57	46979.5
58	40733.0
59	34346.0
60	27901.5
61	22612.0
62	18392.5
63	13940.5
64	9739.5
65	6887.0
66	4997.5
67	3777.5
68	2882.5
69	2098.0
70	1546.5
71	1184.5
72	834.5
73	613.0
74	467.5
75	333.0
76	245.0
77	149.0
78	83.5
79	49.5
80	32.5
81	18.5
82	11.0
83	8.5
84	5.0
85	4.0
86	3.0
87	1.5
88	1.0
89	1.5
90	1.5
91	1.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15992799654326034
2	8.366935544116759E-4
3	0.006633784609978287
4	0.004721342199894457
5	0.028088997898106264
6	0.0015538594581931125
7	1.1952765063023943E-4
8	0.002868663615125746
9	0.0
10-11	0.11107106934814999
12-13	4.1834677720583793E-4
14-15	0.00621543783277245
16-17	2.0917338860291897E-4
18-19	2.9881912657559855E-4
20-21	0.0010757488556721547
22-23	2.689372139180387E-4
24-25	0.0015837413708506723
26-27	0.0027790178771530667
28-29	0.00636484739606025
30-31	0.015150129717382848
32-33	0.015508712669273563
34-35	0.013387096870586814
36-37	9.263392923843555E-4
38-39	1.4940956328779928E-4
40-41	0.004571932636606658
42-43	0.008934691884610397
44-45	0.008815164233980158
46-47	0.009980558827624992
48-49	0.011922883150366381
50-51	0.005677563404936373
52-53	0.0013446860695901935
54-55	8.964573797267956E-4
56-57	0.005976382531511971
58-59	0.009562212050419154
60-61	0.00812788024285628
62-63	0.019124424100838307
64-65	0.03642605152956547
66-67	0.03887636836748537
68-69	0.029642857356299377
70-71	0.04362759248003739
72-73	0.027252304343694588
74-75	0.026206437400679992
76-77	0.24855774948558287
78-79	0.07300151262241872
80-81	0.05698480743796665
82-83	0.021963205803306494
84-85	0.033856207041015315
86-87	0.0836693554411676
88-89	0.26627772369151587
90-91	0.32152938019534405
92-93	0.05546082989243109
94-95	0.005826972968224172
96-97	0.011355126809872745
98-99	0.0020618519733716302
100-101	0.0014940956328779928
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1673253.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.34100217321357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.50841086890878	54.07349662589462
2	10.834622091647846	14.375593754559151
3	3.249372430489096	6.46699870417972
4	1.4592506300935815	3.8723259688920626
5	0.8137443363414874	2.699230739283543
6	0.5007945055061005	1.9933925626868176
7	0.3460762249530155	1.6071330514193884
8	0.23321954891334334	1.23776148810368
9	0.18005242795383608	1.0750372662760037
>10	0.8361348267195472	9.389661738600452
>50	0.02657009507528946	1.1966898490753732
>100	0.010757763554828883	1.332875339186757
>500	7.230907950389851E-4	0.3149867421635669
>1k	2.711590481396194E-4	0.36481616967887703
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2453	0.14660066349798864	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1989	0.11887024855177311	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.1952765063023942E-4	2.9881912657559855E-5
12-13	0.0	0.0	0.0	1.1952765063023942E-4	5.976382531511971E-5
14-15	0.0	0.0	0.0	1.1952765063023942E-4	5.976382531511971E-5
16-17	0.0	0.0	0.0	1.4940956328779928E-4	5.976382531511971E-5
18-19	0.0	5.976382531511971E-5	0.0	1.7929147594535912E-4	1.1952765063023942E-4
20-21	0.0	1.1952765063023942E-4	0.0	1.7929147594535912E-4	1.1952765063023942E-4
22-23	0.0	1.1952765063023942E-4	0.0	2.9881912657559855E-4	1.1952765063023942E-4
24-25	0.0	1.1952765063023942E-4	0.0	2.9881912657559855E-4	1.1952765063023942E-4
26-27	0.0	1.1952765063023942E-4	0.0	6.872839911238767E-4	1.1952765063023942E-4
28-29	0.0	1.1952765063023942E-4	0.0	0.0030180731784135452	1.1952765063023942E-4
30-31	0.0	1.1952765063023942E-4	0.0	0.013118159656668776	1.1952765063023942E-4
32-33	0.0	1.7929147594535912E-4	0.0	0.034184908080248474	1.1952765063023942E-4
34-35	0.0	1.7929147594535912E-4	0.0	0.061945204939121576	1.7929147594535912E-4
36-37	0.0	1.7929147594535912E-4	0.0	0.10360059118376003	1.7929147594535912E-4
38-39	0.0	1.7929147594535912E-4	0.0	0.17056595744935166	1.7929147594535912E-4
40-41	0.0	1.7929147594535912E-4	0.0	0.24960361642859746	1.7929147594535912E-4
42-43	0.0	1.7929147594535912E-4	0.0	0.3313306475470237	1.7929147594535912E-4
44-45	0.0	1.7929147594535912E-4	0.0	0.42683324040058496	1.7929147594535912E-4
46-47	0.0	1.7929147594535912E-4	0.0	0.5344678897931155	2.09173388602919E-4
48-49	0.0	1.7929147594535912E-4	0.0	0.6524117990525042	2.3905530126047884E-4
50-51	0.0	1.7929147594535912E-4	0.0	0.7774974854370499	2.9881912657559855E-4
52-53	0.0	1.7929147594535912E-4	0.0	0.9134601880289472	2.9881912657559855E-4
54-55	0.0	1.7929147594535912E-4	0.0	1.0704597571317667	2.9881912657559855E-4
56-57	0.0	1.7929147594535912E-4	0.0	1.2402189029393642	2.9881912657559855E-4
58-59	0.0	2.09173388602919E-4	0.0	1.41906215019486	2.9881912657559855E-4
60-61	0.0	2.3905530126047884E-4	0.0	1.6126371803905326	2.9881912657559855E-4
62-63	0.0	2.3905530126047884E-4	0.0	1.8168800534049543	2.9881912657559855E-4
64-65	0.0	2.3905530126047884E-4	0.0	2.0440423534277246	3.287010392331584E-4
66-67	0.0	2.3905530126047884E-4	0.0	2.3038357020725497	3.5858295189071824E-4
68-69	0.0	2.3905530126047884E-4	0.0	2.578928589998046	3.5858295189071824E-4
70-71	0.0	2.3905530126047884E-4	0.0	2.8677970396586767	3.5858295189071824E-4
72-73	0.0	2.3905530126047884E-4	0.0	3.1759990868087495	3.5858295189071824E-4
74-75	0.0	2.3905530126047884E-4	0.0	3.496572245799051	3.5858295189071824E-4
76-77	2.9881912657559855E-5	2.3905530126047884E-4	0.0	3.8570377581871957	3.5858295189071824E-4
78-79	5.976382531511971E-5	2.3905530126047884E-4	0.0	4.264925865962888	3.5858295189071824E-4
80-81	5.976382531511971E-5	2.3905530126047884E-4	0.0	4.68273476874089	3.5858295189071824E-4
82-83	1.1952765063023942E-4	2.3905530126047884E-4	0.0	5.124987076072776	3.5858295189071824E-4
84-85	1.1952765063023942E-4	2.3905530126047884E-4	0.0	5.589471526421885	3.5858295189071824E-4
86-87	1.1952765063023942E-4	2.9881912657559855E-4	0.0	6.078578672800826	3.5858295189071824E-4
88-89	1.1952765063023942E-4	3.5858295189071824E-4	0.0	6.610536481930707	3.5858295189071824E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1865	0.0	59.87564	1
GTATCAA	3595	0.0	44.676483	1
TCAACGC	4735	0.0	33.275208	4
CAACGCA	4795	0.0	32.811836	5
ATCAACG	4940	0.0	31.894352	3
AACGCAG	4985	0.0	31.750038	6
TATCAAC	4980	0.0	31.733467	2
GTACATG	5260	0.0	27.282389	1
ACGCAGA	5855	0.0	26.706758	7
TACATGG	5490	0.0	25.6736	2
CGCAGAG	6100	0.0	25.63411	8
ACATGGG	5590	0.0	24.45025	3
CATGGGG	4750	0.0	22.479753	4
GCAGAGT	6735	0.0	22.440973	9
ATGGGGG	3390	0.0	22.434244	5
AGAGTAC	5925	0.0	21.337595	10-11
GAGTACA	4055	0.0	20.624481	1
GAGTACT	3760	0.0	19.685007	12-13
CAGAGTA	6430	0.0	19.366114	10-11
AGTACAT	4200	0.0	19.321882	2
>>END_MODULE