FastQCFastQC Report
Mon 15 Aug 2016
SRR2921387_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921387_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970838
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.047.8959051
GTATCAA30900.044.6856081
TCAACGC38350.035.2651524
TATCAAC39850.034.4131742
CAACGCA39250.033.9703375
ATCAACG40400.033.827223
AACGCAG42350.031.4741346
GTACATG48350.028.6562611
ACGCAGA48600.027.0416137
TACATGG51950.026.2151032
CGCAGAG50100.025.7610978
ACATGGG53050.024.9559483
CATGGGG46800.022.4084954
GCAGAGT56050.021.6719269
TGTACGC1805.2172527E-621.0898112
ATGGGGG31750.020.0260015
AACGTCG600.004792717319.772222-23
AGAGTAC49750.019.40930610-11
GAGTACA37800.018.9547621
GTGTACG2302.0803564E-618.567261
Produced by FastQC (version 0.11.5)