##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921387_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1970838
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.400262730878946	31.0	31.0	34.0	26.0	34.0
2	30.47723404967836	31.0	31.0	34.0	26.0	34.0
3	30.519478008847	33.0	31.0	34.0	26.0	34.0
4	33.98784070532433	37.0	35.0	37.0	30.0	37.0
5	33.986392082961665	37.0	35.0	37.0	30.0	37.0
6	34.03237252376908	37.0	35.0	37.0	30.0	37.0
7	33.95294285983932	37.0	35.0	37.0	30.0	37.0
8	33.91373009856721	37.0	35.0	37.0	30.0	37.0
9	35.47132336599964	39.0	37.0	39.0	30.0	39.0
10-11	35.36305165619904	39.0	35.0	39.0	29.5	39.0
12-13	35.24954410255942	39.0	35.0	39.0	29.0	39.0
14-15	36.380307513859584	40.0	37.0	41.0	28.5	41.0
16-17	36.335235569843896	40.0	37.0	41.0	28.0	41.0
18-19	36.294090128158686	40.0	36.5	41.0	27.5	41.0
20-21	36.235814409910915	40.0	36.5	41.0	27.0	41.0
22-23	36.12133138289398	40.0	36.0	41.0	27.0	41.0
24-25	36.02631291866709	40.0	36.0	41.0	27.0	41.0
26-27	35.87753711872817	40.0	36.0	41.0	27.0	41.0
28-29	35.739600109192125	40.0	36.0	41.0	27.0	41.0
30-31	35.58572241858539	40.0	36.0	41.0	26.0	41.0
32-33	35.4002908914888	39.0	35.0	41.0	25.0	41.0
34-35	35.23058998253535	39.0	35.0	41.0	25.0	41.0
36-37	35.05993668683068	39.0	35.0	41.0	24.0	41.0
38-39	35.2727981701185	39.0	35.0	41.0	25.0	41.0
40-41	35.38408382627085	40.0	35.0	41.0	25.0	41.0
42-43	35.211265461697	40.0	35.0	41.0	24.0	41.0
44-45	35.08983970270514	40.0	35.0	41.0	23.5	41.0
46-47	34.93090426508927	40.0	35.0	41.0	23.0	41.0
48-49	34.77683858338433	39.0	35.0	41.0	22.0	41.0
50-51	33.0631540999311	37.0	32.5	39.5	18.0	40.0
52-53	33.22646813183022	37.5	33.0	39.5	16.5	40.0
54-55	34.09301627023632	39.0	34.0	40.5	18.5	41.0
56-57	34.11761519719023	39.0	34.0	41.0	18.0	41.0
58-59	33.95037796105007	38.5	34.0	41.0	17.5	41.0
60-61	33.71292135629616	38.0	34.0	40.5	14.5	41.0
62-63	33.433799733920296	37.5	34.0	40.0	13.0	41.0
64-65	33.14783888883815	37.0	33.0	40.0	9.5	41.0
66-67	32.8087493746315	36.5	33.0	39.5	7.5	41.0
68-69	32.44931064856675	36.0	33.0	39.0	4.5	41.0
70-71	32.064589529936	35.5	32.0	39.0	2.0	41.0
72-73	31.70057483161985	35.0	32.0	38.5	2.0	40.0
74-75	31.293834906775697	35.0	32.0	37.0	2.0	39.5
76-77	30.902588898732418	35.0	31.5	37.0	2.0	39.0
78-79	30.545510843610685	35.0	31.0	36.0	2.0	39.0
80-81	30.184152375791413	35.0	31.0	36.0	2.0	37.0
82-83	29.869182043374444	35.0	31.0	35.5	2.0	37.0
84-85	29.619327666708273	35.0	31.0	35.0	2.0	36.5
86-87	29.3778938197863	35.0	31.0	35.0	2.0	36.0
88-89	29.161257292583155	35.0	30.0	35.0	2.0	36.0
90-91	28.891684400239896	34.0	30.0	35.0	2.0	36.0
92-93	28.69343954196134	34.0	30.0	35.0	2.0	35.0
94-95	28.480077256476687	34.0	30.0	35.0	2.0	35.0
96-97	28.216911790821975	34.0	29.0	35.0	2.0	35.0
98-99	27.983992088644527	34.0	29.0	35.0	2.0	35.0
100-101	26.932404134687886	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64510.0
3	11387.0
4	7704.0
5	5815.0
6	5332.0
7	5479.0
8	5944.0
9	6440.0
10	6933.0
11	7804.0
12	7745.0
13	8154.0
14	8748.0
15	9542.0
16	9948.0
17	10437.0
18	9782.0
19	8732.0
20	8946.0
21	9274.0
22	10072.0
23	11289.0
24	13333.0
25	15422.0
26	18258.0
27	21467.0
28	25264.0
29	29887.0
30	35572.0
31	42983.0
32	53533.0
33	68747.0
34	95953.0
35	137746.0
36	224458.0
37	407404.0
38	452999.0
39	87778.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.15986642346179	19.268842074375815	13.487920390170204	25.083371111992193
2	15.804472379540476	19.932525912029835	39.55915913781418	24.703842570615517
3	19.063071277107184	23.65689436440607	29.72217244325494	27.557861915231808
4	13.384187746709483	15.65639001358092	36.596240496528615	34.36318174318098
5	14.094450694272407	37.13306515479493	34.60609838808589	14.166385762846781
6	33.61877913305607	35.15068625236934	16.993227555652542	14.237307058922042
7	28.654973727933648	31.988435622019207	21.085451963782408	18.271138686264738
8	26.285502002875532	35.029285679122744	20.090671162500122	18.594541155501602
9	26.780513279087952	15.63963989567754	19.49265870528702	38.087188119947484
10-11	25.43352381136052	25.279113029960765	28.173078118660055	21.114285040018657
12-13	25.84948318036372	22.797771779168503	28.016189646959823	23.33655539350795
14-15	23.043259761619524	24.61430688947999	25.208379865581026	27.13405348331946
16-17	23.2051859882491	27.686790185115257	26.09710430040726	23.01091952622838
18-19	23.22264526698512	27.059890213624637	27.481358850003957	22.236105669386287
20-21	23.515454378888716	26.255695247840034	27.54505317256455	22.6837972007067
22-23	23.402218782053282	26.069432694482288	27.197099089810493	23.331249433653937
24-25	23.253619362829703	26.19855225486812	27.238421084692078	23.309407297610104
26-27	23.0343448780619	26.446880832150306	27.246638828842045	23.272135460945755
28-29	23.091176130262056	26.821076436185436	27.015983329510135	23.07176410404237
30-31	23.580552238006902	26.370616998078994	26.904672554776344	23.14415820913776
32-33	23.21246297200779	26.3812852439883	26.86694026679184	23.53931151721207
34-35	23.51360176835548	26.53537966893572	26.923019347223715	23.02799921548509
36-37	23.453935184010636	26.495451108934905	26.979160852644345	23.071452854410115
38-39	23.34702980282412	26.542260945018338	26.965434914706808	23.14527433745073
40-41	23.551999342385844	26.338588580398277	26.941300071723578	23.168112005492297
42-43	23.305707621426457	26.397586994547495	26.75866309286089	23.538042291165155
44-45	23.210781715559957	26.429042259958702	26.914530611975124	23.44564541250622
46-47	23.35584177601197	26.429857274220048	26.66867845247741	23.545622497290573
48-49	23.144617570112842	26.594490626071693	26.725667406357957	23.53522439745751
50-51	23.3544993398016	26.5497629946213	26.7725068676395	23.323230797937597
52-53	23.52832007614662	26.41402082235912	26.72133264725443	23.336326454239824
54-55	22.991809186249686	26.273956395147085	27.040744080478895	23.693490338124338
56-57	23.13862657659639	26.46312289334788	26.758947256816988	23.639303273238742
58-59	23.199763858443205	26.447167903053824	26.88962822959427	23.4634400089087
60-61	23.290216251564956	26.35768718732615	27.013520426584	23.33857613452489
62-63	23.26512368428212	26.332104368060055	26.98921644628601	23.413555501371814
64-65	23.036846760789846	26.092065924024293	27.050222561340714	23.820864753845147
66-67	23.010419460506597	26.36173082276494	26.7919323860018	23.835917330726666
68-69	23.105174969601396	26.22900475369823	26.969604448275852	23.696215828424517
70-71	23.04362181632216	26.045238135785574	26.97080279911251	23.940337248779752
72-73	22.952026068461993	26.31144938584613	26.763437960176912	23.973086585514963
74-75	22.87866401486959	26.360367835165594	26.827813162186676	23.933154987778142
76-77	22.857242355233094	26.04105051536198	26.913234796573793	24.188472332831136
78-79	22.871751425214416	25.98994588495003	26.983839221626088	24.154463468209475
80-81	22.971630751203794	26.17060912869148	26.76475015816637	24.093009961938357
82-83	22.97538830755805	26.11818565783876	26.912791219543703	23.99363481505949
84-85	22.91838880406219	26.229393150777234	26.747973164533146	24.10424488062743
86-87	22.824262797208736	26.315070698377745	26.728027858713617	24.132638645699895
88-89	22.717638980723258	25.959717206652765	26.92363053761253	24.399013275011445
90-91	22.832407353484573	25.8220842061415	26.980510554949593	24.36499788542433
92-93	22.906172798061473	25.748653758413532	27.068191771331957	24.276981672193035
94-95	22.89540383223816	25.634155677839683	27.05015330733065	24.42028718259151
96-97	22.68086574026266	25.5498881122225	27.098156535044858	24.67108961246998
98-99	22.517174413506368	25.47270964585052	27.105359800529598	24.90475614011351
100-101	22.677523841740946	25.322796633987473	27.244633621210408	24.75504590306118
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	91.0
1	83.0
2	116.5
3	170.0
4	156.5
5	154.0
6	233.0
7	444.0
8	684.5
9	739.0
10	634.5
11	560.5
12	572.5
13	626.0
14	702.5
15	823.5
16	915.0
17	1004.5
18	1175.0
19	1285.5
20	1375.0
21	1502.5
22	1670.0
23	1991.5
24	2367.5
25	2869.5
26	3728.5
27	4984.0
28	6734.5
29	9163.5
30	12490.5
31	16715.5
32	22096.0
33	26094.5
34	29259.5
35	33559.5
36	38715.5
37	44268.0
38	49656.0
39	54111.5
40	57875.0
41	61854.5
42	65596.5
43	69863.5
44	73956.5
45	78576.5
46	81692.5
47	83657.0
48	86216.5
49	86498.5
50	85057.5
51	83253.0
52	81317.5
53	79109.5
54	76144.5
55	71284.5
56	64786.0
57	57674.0
58	51210.0
59	44450.0
60	36240.0
61	29185.5
62	23747.0
63	17930.0
64	12599.0
65	9267.0
66	6866.0
67	5144.0
68	3988.5
69	2977.5
70	2218.5
71	1654.5
72	1160.5
73	810.5
74	611.0
75	470.0
76	339.0
77	231.5
78	160.5
79	124.0
80	104.0
81	79.5
82	56.5
83	52.5
84	42.0
85	34.0
86	29.0
87	24.5
88	25.0
89	21.0
90	17.5
91	15.5
92	12.0
93	9.5
94	8.5
95	7.0
96	5.5
97	4.5
98	4.5
99	4.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05231277253635256
2	0.06053262622295693
3	0.05170389448549297
4	0.059010431095807975
5	0.05104427659706176
6	0.017911162662786084
7	0.015424910621776117
8	0.021361471617657057
9	0.020752593566797473
10-11	0.022553857800590407
12-13	0.02874411798432951
14-15	0.04454957738789287
16-17	0.030748341568408968
18-19	0.023644764308380497
20-21	0.02816060985225574
22-23	0.04939523187598372
24-25	0.04454957738789287
26-27	0.03130647978169692
28-29	0.020803333404369104
30-31	0.013014768337123598
32-33	0.010173337433112209
34-35	0.009818158570110785
36-37	0.001420715452005695
38-39	0.0068752479909561315
40-41	0.003932337411801478
42-43	0.22799946012812822
44-45	0.20054920800187537
46-47	0.2077542649370471
48-49	0.2043039559821761
50-51	0.203213049474386
52-53	0.20833777306912085
54-55	0.2060798502971832
56-57	0.21815593163923164
58-59	0.21417285438985853
60-61	0.21980497636030966
62-63	0.21709039505022737
64-65	0.21825741131437493
66-67	0.2072976063989024
68-69	0.20567393159661018
70-71	0.18847312665982693
72-73	0.1960333624580001
74-75	0.19636317140221568
76-77	0.19395302911756318
78-79	0.2222151186449622
80-81	0.23106922030121196
82-83	0.22916647639227578
84-85	0.2237880536096828
86-87	0.24225735448575683
88-89	0.24040535041439226
90-91	0.23961888293203196
92-93	0.2440078788819781
94-95	0.25448565534052014
96-97	0.25517064314773713
98-99	0.24517489514612567
100-101	0.254231956152662
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1970838.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.26337140273016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.9619410771178	54.31074542481743
2	10.484546644574804	13.894828165974172
3	3.1413088107039724	6.244611372430377
4	1.4038602248506944	3.720980459072074
5	0.8094956035340917	2.681995391292836
6	0.4937482432258701	1.9630453932192826
7	0.35635688318074066	1.652938595148732
8	0.2506066733566379	1.3284834458106862
9	0.18419544701434531	1.0984870184583122
>10	0.8700257737009883	9.62764077307398
>50	0.030304532183017003	1.3511173554167377
>100	0.012457402421979687	1.5138261072029116
>500	9.98992917074565E-4	0.45227580374578935
>1k	1.5369121801147057E-4	0.1590246943366464
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.073983757163197E-5
2	0.0	0.0	0.0	0.0	5.073983757163197E-5
3	0.0	0.0	0.0	0.0	5.073983757163197E-5
4	0.0	0.0	0.0	5.073983757163197E-5	5.073983757163197E-5
5	0.0	0.0	0.0	5.073983757163197E-5	5.073983757163197E-5
6	0.0	0.0	0.0	5.073983757163197E-5	1.0147967514326394E-4
7	0.0	0.0	0.0	5.073983757163197E-5	1.0147967514326394E-4
8	0.0	0.0	0.0	5.073983757163197E-5	1.0147967514326394E-4
9	0.0	0.0	0.0	5.073983757163197E-5	1.0147967514326394E-4
10-11	0.0	0.0	0.0	5.073983757163197E-5	1.0147967514326394E-4
12-13	0.0	0.0	0.0	1.0147967514326394E-4	1.522195127148959E-4
14-15	0.0	0.0	0.0	1.0147967514326394E-4	1.522195127148959E-4
16-17	0.0	0.0	0.0	1.0147967514326394E-4	1.522195127148959E-4
18-19	0.0	0.0	0.0	1.0147967514326394E-4	1.522195127148959E-4
20-21	0.0	5.073983757163197E-5	0.0	1.268495939290799E-4	1.522195127148959E-4
22-23	0.0	5.073983757163197E-5	0.0	3.0443902542979183E-4	1.7758943150071187E-4
24-25	0.0	5.073983757163197E-5	0.0	7.103577260028475E-4	2.536991878581598E-4
26-27	0.0	5.073983757163197E-5	0.0	0.0011162764265759033	2.536991878581598E-4
28-29	0.0	5.073983757163197E-5	0.0	0.004186036599659637	2.536991878581598E-4
30-31	0.0	5.073983757163197E-5	0.0	0.014765292733344901	2.536991878581598E-4
32-33	0.0	5.073983757163197E-5	0.0	0.03955170338708712	2.536991878581598E-4
34-35	0.0	7.610975635744796E-5	0.0	0.07245648805229045	2.536991878581598E-4
36-37	0.0	1.0147967514326394E-4	0.0	0.12464241099471392	2.536991878581598E-4
38-39	0.0	1.0147967514326394E-4	0.0	0.2061813299723265	3.044390254297918E-4
40-41	0.0	1.0147967514326394E-4	0.0	0.29406272864639305	3.044390254297918E-4
42-43	2.5369918785815984E-5	1.0147967514326394E-4	0.0	0.392092094834786	3.044390254297918E-4
44-45	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.512041070854124	3.044390254297918E-4
46-47	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.6499519493738197	3.044390254297918E-4
48-49	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.7857571246342926	3.044390254297918E-4
50-51	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.9272451617027884	3.5517886300142373E-4
52-53	5.073983757163197E-5	1.0147967514326394E-4	0.0	1.077409710996033	4.8202845693050365E-4
54-55	5.073983757163197E-5	1.0147967514326394E-4	0.0	1.2578659433195423	6.088780508595836E-4
56-57	5.073983757163197E-5	1.0147967514326394E-4	0.0	1.4610789927939283	6.088780508595836E-4
58-59	5.073983757163197E-5	1.0147967514326394E-4	0.0	1.6733998431124224	6.088780508595836E-4
60-61	5.073983757163197E-5	1.0147967514326394E-4	0.0	1.8884606446597842	6.088780508595836E-4
62-63	5.073983757163197E-5	1.0147967514326394E-4	0.0	2.1181091495089905	6.088780508595836E-4
64-65	5.073983757163197E-5	1.522195127148959E-4	0.0	2.382210004069335	6.088780508595836E-4
66-67	5.073983757163197E-5	1.522195127148959E-4	0.0	2.6927885498452944	6.088780508595836E-4
68-69	5.073983757163197E-5	1.522195127148959E-4	0.0	3.023561550974763	6.088780508595836E-4
70-71	5.073983757163197E-5	1.522195127148959E-4	0.0	3.3587489179729637	6.849878072170315E-4
72-73	5.073983757163197E-5	1.7758943150071187E-4	0.0	3.7059616264756414	7.610975635744795E-4
74-75	5.073983757163197E-5	2.0295935028652787E-4	0.0	4.0809290261300015	7.610975635744795E-4
76-77	5.073983757163197E-5	2.0295935028652787E-4	0.0	4.515287405661956	7.864674823602955E-4
78-79	5.073983757163197E-5	2.0295935028652787E-4	0.0	4.999218606501397	8.625772387177435E-4
80-81	5.073983757163197E-5	2.0295935028652787E-4	0.0	5.488198421179214	9.386869950751913E-4
82-83	5.073983757163197E-5	2.0295935028652787E-4	0.0	5.99907247576919	9.640569138610073E-4
84-85	5.073983757163197E-5	2.0295935028652787E-4	0.0	6.541024680871791	9.640569138610073E-4
86-87	5.073983757163197E-5	2.0295935028652787E-4	0.0	7.143331922765849	9.640569138610073E-4
88-89	5.073983757163197E-5	2.536991878581598E-4	0.0	7.782019628198766	9.640569138610073E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1595	0.0	47.895905	1
GTATCAA	3090	0.0	44.685608	1
TCAACGC	3835	0.0	35.265152	4
TATCAAC	3985	0.0	34.413174	2
CAACGCA	3925	0.0	33.970337	5
ATCAACG	4040	0.0	33.82722	3
AACGCAG	4235	0.0	31.474134	6
GTACATG	4835	0.0	28.656261	1
ACGCAGA	4860	0.0	27.041613	7
TACATGG	5195	0.0	26.215103	2
CGCAGAG	5010	0.0	25.761097	8
ACATGGG	5305	0.0	24.955948	3
CATGGGG	4680	0.0	22.408495	4
GCAGAGT	5605	0.0	21.671926	9
TGTACGC	180	5.2172527E-6	21.089811	2
ATGGGGG	3175	0.0	20.026001	5
AACGTCG	60	0.0047927173	19.7722	22-23
AGAGTAC	4975	0.0	19.409306	10-11
GAGTACA	3780	0.0	18.954762	1
GTGTACG	230	2.0803564E-6	18.56726	1
>>END_MODULE