FastQCFastQC Report
Mon 15 Aug 2016
SRR2921387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970838
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17650.049.5488431
GTATCAA29650.043.120921
TATCAAC40950.031.409292
TCAACGC41000.031.3709854
CAACGCA42450.030.2459565
ATCAACG42500.030.15213
AACGCAG45750.028.0628536
GTACATG50400.025.7449781
TACATGG52750.023.663342
ACATGGG51350.023.2917883
ACGCAGA53950.023.0923947
CGCAGAG54800.022.3871238
CATGGGG46550.020.59564
ATACCGT2201.3319932E-619.452666
AGAGTAC52100.019.16640910-11
GCAGAGT61050.019.00489
ATGGGGG28900.018.7580725
ACCGTCG1600.00118288917.8316048
GAGTACT32250.017.7287812-13
CAGAGTA58150.016.2728089
Produced by FastQC (version 0.11.5)