##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921387_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1970838
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45504602610666	33.0	31.0	34.0	29.0	34.0
2	31.587570870868127	34.0	31.0	34.0	29.0	34.0
3	31.66240198331877	34.0	31.0	34.0	28.0	34.0
4	35.161112684046074	37.0	35.0	37.0	33.0	37.0
5	34.91049847831227	37.0	35.0	37.0	32.0	37.0
6	34.87259277525601	37.0	35.0	37.0	32.0	37.0
7	34.74302251123634	37.0	35.0	37.0	32.0	37.0
8	34.70264730028546	37.0	35.0	37.0	32.0	37.0
9	36.330388393160675	39.0	37.0	39.0	32.0	39.0
10-11	36.182203712329475	39.0	37.0	39.0	32.0	39.0
12-13	36.11631955543784	39.0	37.0	39.0	32.0	39.0
14-15	37.35837496537006	40.0	38.0	41.0	32.0	41.0
16-17	37.342273692713455	40.0	38.0	41.0	32.0	41.0
18-19	37.31001990016429	40.0	38.0	41.0	31.5	41.0
20-21	37.27616729533325	40.0	38.0	41.0	31.0	41.0
22-23	37.17036737671995	40.0	38.0	41.0	31.0	41.0
24-25	37.10688397524302	40.0	37.5	41.0	31.0	41.0
26-27	36.97114932835677	40.0	37.0	41.0	30.5	41.0
28-29	36.81488382099391	40.0	37.0	41.0	30.0	41.0
30-31	36.66941118448092	40.0	37.0	41.0	30.0	41.0
32-33	36.603533623768165	40.0	37.0	41.0	30.0	41.0
34-35	36.72110290140539	40.0	37.0	41.0	30.0	41.0
36-37	36.688571815643904	40.0	37.0	41.0	30.0	41.0
38-39	36.597831227122676	40.0	37.0	41.0	30.0	41.0
40-41	36.46918290595168	40.0	37.0	41.0	30.0	41.0
42-43	36.36306560965437	40.0	36.5	41.0	29.5	41.0
44-45	36.214845410936874	40.0	36.0	41.0	29.0	41.0
46-47	36.05019387691936	40.0	36.0	41.0	28.0	41.0
48-49	35.882174993581415	40.0	35.5	41.0	28.0	41.0
50-51	35.729947616191694	40.0	35.0	41.0	27.5	41.0
52-53	35.561317317811	39.0	35.0	41.0	27.0	41.0
54-55	35.35953817614639	39.0	35.0	41.0	26.5	41.0
56-57	35.1048701110898	39.0	35.0	41.0	26.0	41.0
58-59	34.88556745912145	39.0	35.0	41.0	26.0	41.0
60-61	34.61963235943289	38.0	34.5	40.0	25.5	41.0
62-63	34.33061799092569	37.5	34.0	40.0	25.0	41.0
64-65	34.02326928951035	37.0	34.0	40.0	24.5	41.0
66-67	33.68867684710768	36.5	34.0	39.5	23.0	41.0
68-69	33.330374693404536	36.0	34.0	39.0	22.5	41.0
70-71	32.9421677479326	35.5	33.0	39.0	22.0	40.0
72-73	32.54251719319396	35.0	33.0	38.0	21.0	40.0
74-75	32.12619707961791	35.0	33.0	37.0	20.0	39.0
76-77	29.339466511199806	33.0	29.0	34.5	13.0	36.5
78-79	31.001136826060794	34.5	31.5	36.0	16.5	38.0
80-81	31.194728333835656	35.0	32.0	36.0	18.0	37.0
82-83	31.023892882114104	35.0	32.0	35.0	17.5	37.0
84-85	30.783373620764365	35.0	32.0	35.0	13.5	36.5
86-87	30.530016926809814	35.0	32.0	35.0	8.5	36.0
88-89	30.239938543908735	35.0	32.0	35.0	2.0	36.0
90-91	30.046151687759217	34.5	31.0	35.0	2.0	35.5
92-93	29.901909238608145	34.0	31.0	35.0	2.0	35.0
94-95	29.80080503826291	34.0	31.0	35.0	2.0	35.0
96-97	29.609164477242675	34.0	31.0	35.0	2.0	35.0
98-99	29.341175682628403	34.0	31.0	35.0	2.0	35.0
100-101	28.248995097516897	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33254.0
3	11328.0
4	6407.0
5	4513.0
6	4060.0
7	3991.0
8	4318.0
9	4565.0
10	5176.0
11	5685.0
12	6241.0
13	6862.0
14	7616.0
15	8360.0
16	9455.0
17	10620.0
18	9508.0
19	8229.0
20	7682.0
21	7443.0
22	7795.0
23	8740.0
24	10174.0
25	12289.0
26	14708.0
27	17561.0
28	21079.0
29	25555.0
30	30870.0
31	38192.0
32	48583.0
33	64089.0
34	92588.0
35	136284.0
36	234381.0
37	457160.0
38	513720.0
39	71754.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.55237196935355	20.421175241343843	13.787141744912418	25.23931104439019
2	15.373167650177844	21.504340810724415	37.82477433365638	25.297717205441362
3	17.958353968936986	24.822499979702688	29.57262988251914	27.646516168841185
4	13.2176314243178	16.956707940209686	35.591809023348425	34.23385161212409
5	13.996120379882008	37.588210096351006	34.43076573266149	13.984903791105499
6	33.49116989671786	35.85689169201574	16.767885367221176	13.884053044045231
7	28.523653390080767	32.38028696422537	21.0120770961388	18.08398254955506
8	26.384780994916273	35.65249424218299	19.756555589890194	18.20616917301054
9	26.71822848960696	15.726558956139469	19.44573831030252	38.109474243951055
10-11	25.43339153623925	25.720689545699866	27.9649766659259	20.880942252134986
12-13	25.98275554130328	23.056992058192563	27.939640842769258	23.02061155773489
14-15	22.988949306249033	24.849776936129448	25.187239691161068	26.97403406646045
16-17	23.216797338794297	27.944131322951275	26.080074648524786	22.75899668972964
18-19	23.22917607469731	27.256760335979592	27.561682008733356	21.95238158058974
20-21	23.573833293164032	26.329354458158384	27.608082909441478	22.48872933923611
22-23	23.43075237729427	26.112384966153908	27.337476082448386	23.119386574103437
24-25	23.202613135445407	26.42762819902959	27.288110867979576	23.08164779754543
26-27	23.074409435833946	26.64516931070614	27.325837166408306	22.954584087051607
28-29	23.152390151425774	26.957913210092954	27.10811353216101	22.781583106320262
30-31	23.623694741310562	26.532417407728904	26.94872492975905	22.89516292120149
32-33	23.32486269574341	26.524784544279274	26.891588948672503	23.25876381130481
34-35	23.552726426658136	26.667446458851202	27.040097472331542	22.73972964215912
36-37	23.488234622556543	26.65236639478391	27.095023657605317	22.76437532505423
38-39	23.492705876144758	26.623919936392376	26.99670646878756	22.8866677186753
40-41	23.63302478563868	26.455571466120816	26.944779062462892	22.96662468577761
42-43	23.39850940344423	26.48509720592433	26.798566490120834	23.317826900510607
44-45	23.336298489952128	26.516720743139626	26.900448883778378	23.246531883129865
46-47	23.484584572672983	26.530676970597618	26.682658091446964	23.30208036528244
48-49	23.223231503095505	26.690246625393282	26.782299807165334	23.304222064345883
50-51	23.396176916679394	26.652790132302368	26.76135490048526	23.18967805053298
52-53	23.66041380563115	26.500497639415784	26.659620474785804	23.17946808016726
54-55	23.100152347560474	26.4000436366035	26.937991625323942	23.56181239051208
56-57	23.24245206641324	26.559587440437422	26.664675856025315	23.53328463712402
58-59	23.255716005576776	26.550301990817854	26.816150324318222	23.377831679287148
60-61	23.381290765847364	26.50722634426444	26.87054791531563	23.240934974572564
62-63	23.269281176105498	26.538090391618184	26.891121989887356	23.301506442388963
64-65	23.04431816336143	26.31678990238594	26.85772263639169	23.781169297860934
66-67	23.062998084937806	26.55074102704791	26.62007511023155	23.76618577778274
68-69	23.06326095126537	26.424305879982512	26.76216386442135	23.750269304330768
70-71	23.03470680050252	26.315571742192557	26.729642272502318	23.92007918480261
72-73	23.02702982525279	26.49208101766149	26.51524936680369	23.96563979028203
74-75	22.914452642466433	26.601456358474497	26.53075983788014	23.95333116117893
76-77	22.84328622964036	26.22557129069544	26.73326373532752	24.197878744336684
78-79	22.79590317112509	26.283762496036378	26.765515369709025	24.154818963129504
80-81	22.98177494811136	26.41258049180049	26.532183901919108	24.073460658169036
82-83	22.897716084844475	26.459571189905382	26.555360227681028	24.087352497569114
84-85	22.847279779400008	26.468471468022294	26.498543595448933	24.18570515712877
86-87	22.759734868508247	26.565376199609304	26.41758159171314	24.25730734016931
88-89	22.644798329784194	26.267490921966047	26.52965762407325	24.558053124176517
90-91	22.639297650521275	26.171201757502523	26.61602377270557	24.57347681927063
92-93	22.63676086032794	26.083342175147106	26.717922111150994	24.56197485337396
94-95	22.539796669925177	26.048053608384482	26.711105213710713	24.70104450797963
96-97	22.364970726478106	25.905880711191177	26.79764796924853	24.931500593082188
98-99	22.19679837061836	25.89124567626797	26.7294801271965	25.182475825917173
100-101	22.156459756339196	25.87626886054921	26.725080785168153	25.242190597943438
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	103.0
1	86.5
2	89.0
3	119.0
4	111.5
5	114.0
6	180.5
7	411.5
8	653.0
9	688.5
10	568.5
11	454.0
12	439.0
13	485.5
14	523.0
15	551.5
16	617.0
17	710.5
18	826.5
19	945.0
20	1050.0
21	1155.5
22	1383.0
23	1738.0
24	2116.5
25	2730.0
26	3700.0
27	5028.0
28	6746.0
29	9144.0
30	12620.5
31	17003.5
32	22583.0
33	26668.0
34	29514.0
35	33506.0
36	38944.5
37	44578.5
38	49744.5
39	54836.0
40	58838.5
41	62728.0
42	66674.5
43	70810.0
44	74995.5
45	79900.0
46	83031.5
47	84850.5
48	87253.0
49	87737.0
50	86217.5
51	83888.5
52	81783.0
53	79499.0
54	76441.5
55	71175.0
56	64203.0
57	56500.5
58	49796.0
59	43343.5
60	35326.5
61	28490.5
62	22970.0
63	17017.5
64	11797.0
65	8539.0
66	6373.5
67	4764.0
68	3522.0
69	2607.0
70	1950.5
71	1396.0
72	944.5
73	668.0
74	496.5
75	346.5
76	216.0
77	125.5
78	83.5
79	53.0
80	25.0
81	14.0
82	9.5
83	6.5
84	4.5
85	3.0
86	4.5
87	3.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15759793549748888
2	4.0591870057305574E-4
3	0.006799138234598683
4	0.004262146356017085
5	0.027500991963824526
6	0.0011670162641475353
7	0.0
8	0.0019788536652936466
9	0.0
10-11	0.10911602069779455
12-13	1.0147967514326394E-4
14-15	0.0051754634323064605
16-17	1.0147967514326394E-4
18-19	2.0295935028652787E-4
20-21	6.596178884312156E-4
22-23	2.536991878581598E-4
24-25	0.0012938658580766151
26-27	0.0026892113912964943
28-29	0.0069259878285277635
30-31	0.015196581352703773
32-33	0.015501020378133565
34-35	0.011543313047546272
36-37	6.596178884312156E-4
38-39	2.536991878581598E-4
40-41	0.0050739837571631965
42-43	0.007636345554530611
44-45	0.008727252062320699
46-47	0.010071857757968945
48-49	0.012076081342048408
50-51	0.006342479696453996
52-53	0.0016997845586496708
54-55	7.864674823602956E-4
56-57	0.005911191077095124
58-59	0.00839744311810509
60-61	0.006697658559455419
62-63	0.01697247566771089
64-65	0.03196609767012814
66-67	0.033843471660278526
68-69	0.024532711465884056
70-71	0.038714496067155185
72-73	0.024228272440454265
74-75	0.02252848788180459
76-77	0.24281549269904476
78-79	0.06918376852892018
80-81	0.05046076846498799
82-83	0.01836782120093077
84-85	0.029175406603688378
86-87	0.07869748807360118
88-89	0.25897613096560956
90-91	0.3162106677464104
92-93	0.05231277253635256
94-95	0.005150093513520644
96-97	0.010173337433112209
98-99	0.001471455289577327
100-101	0.0012431260205049831
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1970838.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.5311667870815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.56624857245392	53.451314393980255
2	10.681011541721404	13.998782975905755
3	3.152559891295803	6.197727841283063
4	1.4697440475296843	3.852561692519519
5	0.8511926367059935	2.7889823321958063
6	0.5078547645340578	1.9968189166977204
7	0.35829910810240145	1.6435831028904706
8	0.2656446786998815	1.3926404596786088
9	0.19639076243353365	1.1582744227626594
>10	0.904449509628902	9.945146458424563
>50	0.033309429490556215	1.457090755761523
>100	0.012284814870955152	1.522833972538619
>500	7.771096407410425E-4	0.36436767008403537
>1k	2.3313289222231273E-4	0.22987500527733556
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.073983757163197E-5
2	0.0	0.0	0.0	0.0	5.073983757163197E-5
3	0.0	0.0	0.0	0.0	5.073983757163197E-5
4	0.0	0.0	0.0	0.0	5.073983757163197E-5
5	0.0	5.073983757163197E-5	0.0	0.0	5.073983757163197E-5
6	0.0	5.073983757163197E-5	0.0	0.0	1.0147967514326394E-4
7	0.0	5.073983757163197E-5	0.0	0.0	1.0147967514326394E-4
8	0.0	5.073983757163197E-5	0.0	0.0	1.0147967514326394E-4
9	0.0	5.073983757163197E-5	0.0	0.0	1.522195127148959E-4
10-11	0.0	5.073983757163197E-5	0.0	0.0	1.522195127148959E-4
12-13	0.0	5.073983757163197E-5	0.0	0.0	1.7758943150071187E-4
14-15	0.0	5.073983757163197E-5	0.0	0.0	2.7906910664397583E-4
16-17	0.0	5.073983757163197E-5	0.0	0.0	3.044390254297918E-4
18-19	0.0	5.073983757163197E-5	0.0	0.0	3.5517886300142373E-4
20-21	0.0	5.073983757163197E-5	0.0	5.073983757163197E-5	3.5517886300142373E-4
22-23	0.0	5.073983757163197E-5	0.0	2.2832926907234382E-4	3.5517886300142373E-4
24-25	0.0	1.0147967514326394E-4	0.0	6.088780508595836E-4	3.5517886300142373E-4
26-27	0.0	1.0147967514326394E-4	0.0	0.0010147967514326393	3.5517886300142373E-4
28-29	0.0	1.0147967514326394E-4	0.0	0.004109926843302189	3.5517886300142373E-4
30-31	0.0	1.0147967514326394E-4	0.0	0.015069731758774695	3.8054878178723974E-4
32-33	0.0	1.0147967514326394E-4	0.0	0.04071871965123465	4.0591870057305574E-4
34-35	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.07425775228608339	4.0591870057305574E-4
36-37	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.12743310206115369	4.0591870057305574E-4
38-39	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.20991070803384143	4.0591870057305574E-4
40-41	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.2986800538654116	4.0591870057305574E-4
42-43	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.39767347696766553	4.0591870057305574E-4
44-45	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.5186118798196503	4.0591870057305574E-4
46-47	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.6574360754156354	4.3128861935887175E-4
48-49	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.7940530880772545	4.566585381446877E-4
50-51	5.073983757163197E-5	1.0147967514326394E-4	0.0	0.936251482871753	4.566585381446877E-4
52-53	5.073983757163197E-5	1.0147967514326394E-4	0.0	1.0874561988352163	5.073983757163197E-4
54-55	5.073983757163197E-5	1.0147967514326394E-4	0.0	1.268800378316229	5.581382132879517E-4
56-57	5.073983757163197E-5	1.0147967514326394E-4	0.0	1.4747533790194831	5.581382132879517E-4
58-59	5.073983757163197E-5	1.0147967514326394E-4	0.0	1.6885456846275544	5.835081320737677E-4
60-61	5.073983757163197E-5	1.0147967514326394E-4	0.0	1.9052047910584229	6.088780508595836E-4
62-63	5.073983757163197E-5	1.0147967514326394E-4	0.0	2.136933629248066	6.088780508595836E-4
64-65	5.073983757163197E-5	1.0147967514326394E-4	0.0	2.40273426836706	6.088780508595836E-4
66-67	5.073983757163197E-5	1.0147967514326394E-4	0.0	2.7146574198386677	6.088780508595836E-4
68-69	5.073983757163197E-5	1.0147967514326394E-4	0.0	3.0463691079632116	6.088780508595836E-4
70-71	5.073983757163197E-5	1.0147967514326394E-4	0.0	3.385057523753855	7.103577260028475E-4
72-73	5.073983757163197E-5	1.268495939290799E-4	0.0	3.7365070086937635	7.103577260028475E-4
74-75	5.073983757163197E-5	1.522195127148959E-4	0.0	4.115609705110211	7.103577260028475E-4
76-77	5.073983757163197E-5	1.522195127148959E-4	0.0	4.553900422053969	7.357276447886635E-4
78-79	5.073983757163197E-5	1.522195127148959E-4	0.0	5.040799903391349	7.610975635744795E-4
80-81	5.073983757163197E-5	1.522195127148959E-4	0.0	5.535564059552333	7.610975635744795E-4
82-83	5.073983757163197E-5	1.522195127148959E-4	0.0	6.054226679209554	8.118374011461115E-4
84-85	5.073983757163197E-5	1.522195127148959E-4	0.0	6.604449477836331	8.118374011461115E-4
86-87	5.073983757163197E-5	1.522195127148959E-4	0.0	7.216118219762355	8.118374011461115E-4
88-89	5.073983757163197E-5	1.522195127148959E-4	0.0	7.864928522790812	8.118374011461115E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1765	0.0	49.548843	1
GTATCAA	2965	0.0	43.12092	1
TATCAAC	4095	0.0	31.40929	2
TCAACGC	4100	0.0	31.370985	4
CAACGCA	4245	0.0	30.245956	5
ATCAACG	4250	0.0	30.1521	3
AACGCAG	4575	0.0	28.062853	6
GTACATG	5040	0.0	25.744978	1
TACATGG	5275	0.0	23.66334	2
ACATGGG	5135	0.0	23.291788	3
ACGCAGA	5395	0.0	23.092394	7
CGCAGAG	5480	0.0	22.387123	8
CATGGGG	4655	0.0	20.5956	4
ATACCGT	220	1.3319932E-6	19.45266	6
AGAGTAC	5210	0.0	19.166409	10-11
GCAGAGT	6105	0.0	19.0048	9
ATGGGGG	2890	0.0	18.758072	5
ACCGTCG	160	0.001182889	17.831604	8
GAGTACT	3225	0.0	17.72878	12-13
CAGAGTA	5815	0.0	16.272808	9
>>END_MODULE