FastQCFastQC Report
Mon 15 Aug 2016
SRR2921386_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921386_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332926
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.050.5530131
GTATCAA38850.042.636781
TCAACGC48650.033.4663124
ATCAACG50300.032.4614873
CAACGCA50300.032.460795
TATCAAC50850.032.110382
AACGCAG54000.030.4026376
GTACATG78700.026.6561571
TACATGG80350.026.3467432
ACATGGG83550.024.3150563
ACGCAGA66250.023.9261787
CGCAGAG66850.023.4999688
GTGGTAT12650.022.136631
CATGGGG71550.021.8264374
GCAGAGT75800.019.8486399
TGGTATC13700.019.401952
GTAAGTA10000.018.0368862
ATGGGGG43650.017.615685
CGTATAC1650.001471569817.2601783
CAGAGTA69200.017.07824710-11
Produced by FastQC (version 0.11.5)