##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921386_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2332926
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.77709837345891	33.0	31.0	34.0	27.0	34.0
2	30.8492125339595	33.0	31.0	34.0	27.0	34.0
3	30.89348269083546	34.0	31.0	34.0	27.0	34.0
4	34.34964160886372	37.0	35.0	37.0	31.0	37.0
5	34.340484867501154	37.0	35.0	37.0	31.0	37.0
6	34.38654848032042	37.0	35.0	37.0	31.0	37.0
7	34.3137677748887	37.0	35.0	37.0	30.0	37.0
8	34.28400686519847	37.0	35.0	37.0	30.0	37.0
9	35.89074321260083	39.0	37.0	39.0	31.0	39.0
10-11	35.79909221295489	39.0	37.0	39.0	30.0	39.0
12-13	35.70020352124328	39.0	37.0	39.0	30.0	39.0
14-15	36.890441016988966	40.0	38.0	41.0	30.5	41.0
16-17	36.832989559034445	40.0	37.0	41.0	30.0	41.0
18-19	36.78266220188725	40.0	37.0	41.0	30.0	41.0
20-21	36.72486268317126	40.0	37.0	41.0	30.0	41.0
22-23	36.613685774859555	40.0	37.0	41.0	30.0	41.0
24-25	36.516973963169	40.0	37.0	41.0	30.0	41.0
26-27	36.37258275658979	40.0	37.0	41.0	29.0	41.0
28-29	36.2382190862462	40.0	37.0	41.0	28.5	41.0
30-31	36.09630266883733	40.0	36.0	41.0	28.0	41.0
32-33	35.925225660822505	40.0	36.0	41.0	27.0	41.0
34-35	35.758916913781235	40.0	36.0	41.0	27.0	41.0
36-37	35.59735413810811	39.5	35.0	41.0	26.0	41.0
38-39	35.839730878733405	40.0	36.0	41.0	27.5	41.0
40-41	35.9657584509753	40.0	36.0	41.0	27.5	41.0
42-43	35.80779073146769	40.0	36.0	41.0	27.0	41.0
44-45	35.687790139935856	40.0	36.0	41.0	26.0	41.0
46-47	35.538722831328556	40.0	35.0	41.0	26.0	41.0
48-49	35.39896957726049	40.0	35.0	41.0	25.0	41.0
50-51	33.679099765701956	38.0	33.0	39.5	22.0	40.0
52-53	33.84442155473427	37.5	33.5	39.5	22.5	40.0
54-55	34.69884985636064	39.0	35.0	40.5	23.0	41.0
56-57	34.70599088869514	39.0	35.0	41.0	23.0	41.0
58-59	34.52081827713352	39.0	35.0	41.0	23.0	41.0
60-61	34.27305281007627	38.0	34.0	40.5	22.5	41.0
62-63	33.97967980981823	37.5	34.0	40.0	22.0	41.0
64-65	33.682563656112535	37.0	34.0	40.0	21.5	41.0
66-67	33.33585956005463	36.5	34.0	39.5	20.5	41.0
68-69	32.96368123120922	36.0	33.5	39.0	19.5	41.0
70-71	32.58369403915941	35.5	33.0	39.0	18.0	40.5
72-73	32.22338642545884	35.0	33.0	37.5	16.5	40.0
74-75	31.822570668765316	35.0	33.0	37.0	12.0	39.0
76-77	31.450666459201877	35.0	32.5	36.5	7.5	39.0
78-79	31.11229438910621	35.0	32.0	36.0	6.0	38.5
80-81	30.769396028849606	35.0	32.0	36.0	2.0	37.0
82-83	30.48424532111177	35.0	32.0	35.0	2.0	37.0
84-85	30.260751948411567	35.0	32.0	35.0	2.0	36.5
86-87	30.04607282871381	35.0	32.0	35.0	2.0	36.0
88-89	29.859842103864416	35.0	31.0	35.0	2.0	36.0
90-91	29.620538542585578	35.0	31.0	35.0	2.0	35.5
92-93	29.4417255412302	35.0	31.0	35.0	2.0	35.0
94-95	29.25492793170465	35.0	31.0	35.0	2.0	35.0
96-97	29.022600159627867	35.0	31.0	35.0	2.0	35.0
98-99	28.804948378131154	34.5	31.0	35.0	2.0	35.0
100-101	27.775651692338293	33.5	28.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68501.0
3	11875.0
4	8209.0
5	6156.0
6	5783.0
7	5916.0
8	6289.0
9	6665.0
10	7168.0
11	7676.0
12	7832.0
13	8025.0
14	8588.0
15	9118.0
16	9414.0
17	9896.0
18	9033.0
19	8371.0
20	8507.0
21	8906.0
22	9931.0
23	11280.0
24	13175.0
25	15622.0
26	18550.0
27	22243.0
28	26512.0
29	31446.0
30	37126.0
31	45781.0
32	58162.0
33	76582.0
34	109322.0
35	164771.0
36	282111.0
37	528235.0
38	564198.0
39	95933.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.05881575990655	17.777238396975502	13.193792851469965	24.970152991647986
2	17.037650156750566	19.19634777611473	38.08837215286504	25.677629914269662
3	20.066228743892367	22.54165884049096	28.47072667220717	28.921385743409502
4	14.388996437874665	15.66245838969291	34.51320336536359	35.435341807068845
5	15.670694524611845	35.79624739378522	33.08158149100786	15.451476590595071
6	34.81946145611518	32.95956483658007	16.926289651364236	15.294684055940518
7	29.993025037414867	30.605767140852656	20.062941856591856	19.338265965140618
8	27.24830557414552	33.33739192394773	19.543014129468755	19.871288372438
9	26.896822266428867	15.692438949101387	19.21195465770339	38.19878412676636
10-11	26.278100329010833	24.72526695151785	26.885595636512054	22.11103708295926
12-13	26.748971105195302	22.36891693681542	26.604139074764284	24.277972883224997
14-15	24.243888099491134	24.02306399396384	24.133851269743968	27.59919663680106
16-17	24.33258843010358	26.695498080430685	25.081572048806883	23.89034144065885
18-19	24.244200927885835	26.111955973227563	26.301653362053408	23.34218973683319
20-21	24.84908367599773	25.15937351208692	26.344816172458895	23.646726639456457
22-23	24.70957003221151	25.0389537767099	25.92688761521838	24.324588575860215
24-25	24.439989579905085	25.32205275869869	25.953476205841902	24.284481455554328
26-27	24.208107787684195	25.60174032019782	26.02642252674443	24.163729365373555
28-29	24.221622852203843	25.80290072794732	25.946140730193886	24.02933568965495
30-31	24.659728262756786	25.333026668284305	25.88097251975353	24.12627254920538
32-33	24.37971383724437	25.27082962249434	25.80386150173608	24.545595038525207
34-35	24.634320011625967	25.559510423288973	25.754112493242836	24.052057071842224
36-37	24.535796824161217	25.54522909405929	25.84498534117795	24.07398874060154
38-39	24.354640343728846	25.660856261378644	25.97248163019999	24.012021764692516
40-41	24.594196866227175	25.322749966457238	25.95118917132443	24.131863995991157
42-43	24.415057698675106	25.33903957075673	25.723504152561045	24.522398578007124
44-45	24.41615862461447	25.473188066831202	25.761644465520583	24.349008843033737
46-47	24.454027535153166	25.488146882774977	25.59486712353206	24.4629584585398
48-49	24.2185866397712	25.591998684423515	25.818896985294653	24.37051769051063
50-51	24.25193645428731	25.549689821681547	25.988278121227694	24.210095602803445
52-53	24.475199740656496	25.40510078007005	25.819985980851946	24.299713498421504
54-55	24.197125665765633	25.300908139853668	25.94320331353721	24.558762880843492
56-57	24.19218984266019	25.500080622668946	25.810182675294186	24.497546859376673
58-59	24.23292151100378	25.5510263496442	25.91698170244706	24.29907043690496
60-61	24.31968487639437	25.41570817142511	26.12474057826195	24.13986637391857
62-63	24.24273328619863	25.347149685039827	26.11804474132773	24.292072287433818
64-65	24.159714835730387	25.227556432320508	26.010418406682	24.602310325267105
66-67	24.193135287145363	25.412881281992433	25.804492417995352	24.589491012866855
68-69	24.074679559084462	25.390084016114088	25.974600877837972	24.56063554696347
70-71	24.067365337263507	25.20926095224439	26.065301652386086	24.65807205810602
72-73	24.039279749612525	25.35523078838566	25.990151082565184	24.615338379436626
74-75	23.978718823181147	25.360376731097382	26.018973512087545	24.641930933633923
76-77	24.073541789605894	25.188479386029783	25.91706670885508	24.820912115509245
78-79	23.932454908171604	25.227074894025993	26.040920074704644	24.79955012309776
80-81	23.999932142739038	25.36016959161525	25.963948897469823	24.67594936817589
82-83	23.974600766890035	25.334599100578036	26.12779367621711	24.563006456314817
84-85	24.03087059801638	25.362021812457016	25.982860690017084	24.624246899509515
86-87	23.876564775381677	25.49146901996543	25.94698291570555	24.68498328894734
88-89	23.838510909384922	25.282658736967655	26.033637941264708	24.845192412382715
90-91	23.921616545470712	25.17792849188021	26.050573739124683	24.8498812235244
92-93	23.885086451463263	25.054559667724003	26.287451383803262	24.772902497009472
94-95	23.831855392475884	24.995564673927284	26.295577002630267	24.877002930966565
96-97	23.835393120868076	24.98026126439596	26.229214187833723	24.955131426902245
98-99	23.680629067212507	24.967039216406363	26.17063667197776	25.18169504440337
100-101	23.871194860269153	24.86973260065707	26.221184378737266	25.037888160336507
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	22.5
2	32.0
3	52.0
4	52.5
5	49.5
6	96.5
7	243.5
8	374.0
9	407.5
10	397.0
11	334.5
12	333.5
13	394.0
14	454.0
15	512.5
16	564.0
17	634.0
18	688.5
19	784.0
20	915.0
21	1006.0
22	1060.5
23	1170.0
24	1376.0
25	1632.5
26	1994.0
27	2558.5
28	3470.5
29	4949.0
30	7214.0
31	10436.0
32	14832.0
33	19144.0
34	23215.5
35	28086.5
36	34300.0
37	40661.5
38	46694.0
39	52650.5
40	58647.5
41	64830.0
42	70481.0
43	76354.5
44	82130.0
45	88158.0
46	93362.0
47	97594.5
48	101772.5
49	104714.5
50	105488.0
51	105635.0
52	105126.5
53	103995.0
54	102211.0
55	97754.5
56	91111.0
57	82777.0
58	74852.5
59	66143.0
60	55239.5
61	45404.0
62	37157.0
63	29049.5
64	21645.0
65	16014.0
66	12161.0
67	9438.0
68	7355.5
69	5687.5
70	4334.0
71	3225.0
72	2328.0
73	1776.0
74	1401.0
75	1034.0
76	740.0
77	528.0
78	360.0
79	218.5
80	149.0
81	124.0
82	103.5
83	84.5
84	63.0
85	44.5
86	48.0
87	48.0
88	35.0
89	30.5
90	28.0
91	23.5
92	21.0
93	21.5
94	15.0
95	12.5
96	13.0
97	9.5
98	7.0
99	5.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04432202307317078
2	0.051266092452139506
3	0.04320754280247209
4	0.050237341433033024
5	0.04286462579610326
6	0.01615996392513093
7	0.01273079386144267
8	0.018217465963343886
9	0.019117623105062053
10-11	0.020575020382129567
12-13	0.024389972077982755
14-15	0.04198590096728315
16-17	0.02709044350313726
18-19	0.02258965779454642
20-21	0.025311561532598975
22-23	0.042671734980020797
24-25	0.03829954314881827
26-27	0.03049818125392747
28-29	0.020446426504741256
30-31	0.013073710867811495
32-33	0.00996602549759401
34-35	0.009301623797754408
36-37	0.0013073710867811494
38-39	0.0067297462499882115
40-41	0.003557763941076571
42-43	0.18701836234839855
44-45	0.16307846884127486
46-47	0.1688651933237488
48-49	0.16755782223696766
50-51	0.16556461713744886
52-53	0.1701082674718358
54-55	0.1686508701947683
56-57	0.17902410963742527
58-59	0.17531631950606236
60-61	0.17955991745987657
62-63	0.17743811848296945
64-65	0.17844543718917788
66-67	0.16963675658807864
68-69	0.1675363899240696
70-71	0.1542054913014815
72-73	0.1601422419742418
74-75	0.15994935115815934
76-77	0.1582776307521113
78-79	0.1838463800394869
80-81	0.1932765977146296
82-83	0.1899117245896355
84-85	0.1844250524877343
86-87	0.2004564225354769
88-89	0.19857037900044838
90-91	0.1973058725394633
92-93	0.20322119089932555
94-95	0.21539474462541888
96-97	0.21500896299325398
98-99	0.20491434361827165
100-101	0.21526615074803057
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2332926.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.07676442917526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.86338737489896	55.72994537695318
2	10.79698552778986	14.700476806411297
3	3.1851309319844394	6.505002243983523
4	1.3914725179349499	3.7890778725251573
5	0.7633369599122315	2.5982755200013896
6	0.4663407827527786	1.9048182966706868
7	0.31482059868640755	1.5002377413958545
8	0.22662781669039006	1.2342470791943998
9	0.167656781186176	1.0272178077988579
>10	0.7963504054781514	9.019207778634488
>50	0.020178751022718877	0.9017190912030586
>100	0.0072700576006881614	0.8801158933006055
>500	3.153529016046533E-4	0.11833274703235118
>1k	1.2614116063830731E-4	0.091325744895242
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	4.286462579610326E-5
8	0.0	0.0	0.0	0.0	4.286462579610326E-5
9	0.0	0.0	0.0	0.0	1.7145850318441304E-4
10-11	0.0	0.0	0.0	6.429693869415489E-5	1.7145850318441304E-4
12-13	0.0	0.0	0.0	8.572925159220652E-5	1.7145850318441304E-4
14-15	0.0	0.0	0.0	1.285938773883098E-4	1.9289081608246466E-4
16-17	0.0	0.0	0.0	1.285938773883098E-4	2.143231289805163E-4
18-19	0.0	0.0	0.0	1.285938773883098E-4	2.7862006767467124E-4
20-21	0.0	0.0	0.0	1.285938773883098E-4	3.2148469347077443E-4
22-23	0.0	0.0	0.0	1.285938773883098E-4	3.857816321649293E-4
24-25	0.0	0.0	0.0	2.143231289805163E-4	4.286462579610326E-4
26-27	0.0	0.0	0.0	4.07213945062981E-4	4.286462579610326E-4
28-29	0.0	0.0	0.0	0.0017788819705382855	4.5007857085908426E-4
30-31	0.0	0.0	0.0	0.00747987720142002	4.929431966551876E-4
32-33	0.0	0.0	0.0	0.022653954733240576	5.78672448247394E-4
34-35	0.0	0.0	0.0	0.04704392681122333	6.858340127376522E-4
36-37	0.0	0.0	0.0	0.07906380228091248	6.858340127376522E-4
38-39	0.0	0.0	0.0	0.12182126651252548	7.072663256357037E-4
40-41	0.0	0.0	0.0	0.17293733277437862	7.501309514318071E-4
42-43	0.0	0.0	0.0	0.23466239392076732	7.715632643298587E-4
44-45	0.0	0.0	0.0	0.3194914883712556	7.929955772279103E-4
46-47	0.0	0.0	0.0	0.4168370535542062	8.358602030240136E-4
48-49	0.0	0.0	0.0	0.5048809949394023	9.430217675142718E-4
50-51	0.0	0.0	0.0	0.5930963948277828	9.85886393310375E-4
52-53	0.0	0.0	0.0	0.6945783964000573	0.0010501833320045299
54-55	0.0	0.0	0.0	0.8299020200383553	0.0011144802706986848
56-57	0.0	2.143231289805163E-5	0.0	0.9964525235691145	0.0011359125835967365
58-59	0.0	4.286462579610326E-5	0.0	1.1522225737121539	0.0011573448964947882
60-61	0.0	8.572925159220652E-5	0.0	1.3020130085566366	0.0012002095222908914
62-63	0.0	8.572925159220652E-5	0.0	1.4700852063031575	0.0013288033996792011
64-65	0.0	8.572925159220652E-5	0.0	1.6819221869874998	0.0013288033996792011
66-67	0.0	8.572925159220652E-5	0.0	1.9560200366406821	0.0013502357125772528
68-69	0.0	8.572925159220652E-5	0.0	2.2371691172373236	0.0013716680254753043
70-71	0.0	8.572925159220652E-5	0.0	2.4979146359550195	0.0013716680254753043
72-73	2.143231289805163E-5	8.572925159220652E-5	0.0	2.7611034383430937	0.001457397277067511
74-75	4.286462579610326E-5	8.572925159220652E-5	0.0	3.076865704270088	0.001457397277067511
76-77	4.286462579610326E-5	8.572925159220652E-5	0.0	3.4796645928760706	0.001457397277067511
78-79	4.286462579610326E-5	8.572925159220652E-5	0.0	3.922370448098225	0.001457397277067511
80-81	4.286462579610326E-5	8.572925159220652E-5	0.0	4.340021929542557	0.0015216942157616658
82-83	4.286462579610326E-5	8.572925159220652E-5	0.0	4.743549516787073	0.0016717204060480273
84-85	4.286462579610326E-5	8.572925159220652E-5	0.0	5.198428925735321	0.0017145850318441304
86-87	4.286462579610326E-5	1.0716156449025815E-4	0.0	5.749067908712064	0.0017574496576402338
88-89	4.286462579610326E-5	1.285938773883098E-4	0.0	6.356502520868643	0.0018431789092324402
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2150	0.0	50.553013	1
GTATCAA	3885	0.0	42.63678	1
TCAACGC	4865	0.0	33.466312	4
ATCAACG	5030	0.0	32.461487	3
CAACGCA	5030	0.0	32.46079	5
TATCAAC	5085	0.0	32.11038	2
AACGCAG	5400	0.0	30.402637	6
GTACATG	7870	0.0	26.656157	1
TACATGG	8035	0.0	26.346743	2
ACATGGG	8355	0.0	24.315056	3
ACGCAGA	6625	0.0	23.926178	7
CGCAGAG	6685	0.0	23.499968	8
GTGGTAT	1265	0.0	22.13663	1
CATGGGG	7155	0.0	21.826437	4
GCAGAGT	7580	0.0	19.848639	9
TGGTATC	1370	0.0	19.40195	2
GTAAGTA	1000	0.0	18.036886	2
ATGGGGG	4365	0.0	17.61568	5
CGTATAC	165	0.0014715698	17.260178	3
CAGAGTA	6920	0.0	17.078247	10-11
>>END_MODULE