FastQCFastQC Report
Mon 15 Aug 2016
SRR2921386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332926
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24100.046.7400551
GTATCAA38550.038.9601481
TCAACGC49750.030.051754
CAACGCA51350.029.2554745
ATCAACG51650.029.0381573
TATCAAC51150.028.7652652
AACGCAG55750.027.1153246
GTACATG71100.025.0679951
TACATGG74300.023.635522
ACATGGG77500.022.0471823
ACGCAGA69300.021.1275927
GTGGTAT13400.020.9269121
CGCAGAG69850.020.4167848
CATGGGG67350.019.943494
ACGATAG1200.004748193519.8037171
TGGTATC13700.018.7079182
GTAAGTA11450.017.4098932
GCAGAGT77350.017.2079949
TAAGTAC15750.016.8756223
ATGGGGG41150.016.4051065
Produced by FastQC (version 0.11.5)