##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921386_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2332926
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87046867324553	33.0	31.0	34.0	27.0	34.0
2	31.026693517068264	33.0	31.0	34.0	27.0	34.0
3	31.087448551732887	34.0	31.0	34.0	27.0	34.0
4	34.57440656068817	37.0	35.0	37.0	32.0	37.0
5	34.26848815607524	37.0	35.0	37.0	30.0	37.0
6	34.20559674846095	37.0	35.0	37.0	30.0	37.0
7	34.05287823102833	37.0	35.0	37.0	30.0	37.0
8	34.0109326228093	37.0	35.0	37.0	30.0	37.0
9	35.56665663634423	39.0	37.0	39.0	30.0	39.0
10-11	35.406365868441604	39.0	37.0	39.0	30.0	39.0
12-13	35.32118506973646	39.0	37.0	39.0	29.0	39.0
14-15	36.48804205534166	40.0	37.0	41.0	29.0	41.0
16-17	36.44669762349942	40.0	37.0	41.0	29.0	41.0
18-19	36.39526735953048	40.0	37.0	41.0	28.5	41.0
20-21	36.34970483418677	40.0	37.0	41.0	28.0	41.0
22-23	36.227392982032	40.0	37.0	41.0	27.0	41.0
24-25	36.15002211814691	40.0	37.0	41.0	27.0	41.0
26-27	35.996106177392676	40.0	36.0	41.0	27.0	41.0
28-29	35.8313607032542	40.0	36.0	41.0	27.0	41.0
30-31	35.685215047541156	40.0	36.0	41.0	26.5	41.0
32-33	35.63731897196911	40.0	36.0	41.0	26.5	41.0
34-35	35.77257036871294	40.0	36.0	41.0	27.0	41.0
36-37	35.74250897799587	40.0	36.0	41.0	26.0	41.0
38-39	35.648172938190065	40.0	36.0	41.0	25.5	41.0
40-41	35.52206413748229	40.0	35.0	41.0	25.0	41.0
42-43	35.40850567056135	40.0	35.0	41.0	25.0	41.0
44-45	35.25842418490771	40.0	35.0	41.0	24.0	41.0
46-47	35.097744420525984	40.0	35.0	41.0	24.0	41.0
48-49	34.933663348087336	39.0	35.0	41.0	23.0	41.0
50-51	34.767220863413584	39.0	35.0	41.0	23.0	41.0
52-53	34.58974030895108	39.0	35.0	41.0	22.0	41.0
54-55	34.37132596576145	39.0	34.5	41.0	21.5	41.0
56-57	34.10220169863939	38.0	34.0	40.0	20.0	41.0
58-59	33.865512450887856	38.0	34.0	40.0	19.5	41.0
60-61	33.58514243486506	37.5	34.0	40.0	18.0	41.0
62-63	33.286954879837594	37.0	33.5	40.0	16.0	41.0
64-65	32.96778337589791	36.5	33.0	39.5	13.5	41.0
66-67	32.6353251239002	36.0	33.0	39.0	12.0	41.0
68-69	32.27415786012929	35.5	33.0	39.0	8.0	40.5
70-71	31.895306152016822	35.0	32.0	38.0	7.0	40.0
72-73	31.506127498257555	35.0	32.0	37.0	2.0	39.5
74-75	31.105904773661916	35.0	32.0	37.0	2.0	39.0
76-77	28.43178137669176	32.0	27.5	34.5	2.0	36.0
78-79	30.053771101183663	34.5	30.5	35.0	2.0	37.0
80-81	30.26343270210885	35.0	31.0	35.0	2.0	37.0
82-83	30.117858003211417	35.0	31.0	35.0	2.0	37.0
84-85	29.90035024685738	35.0	31.0	35.0	2.0	36.0
86-87	29.662936801253018	34.5	31.0	35.0	2.0	36.0
88-89	29.38752000706409	34.0	31.0	35.0	2.0	35.5
90-91	29.2076752541658	34.0	31.0	35.0	2.0	35.0
92-93	29.066149547821063	34.0	30.0	35.0	2.0	35.0
94-95	28.966696543310846	34.0	30.0	35.0	2.0	35.0
96-97	28.769218354975685	34.0	30.0	35.0	2.0	35.0
98-99	28.491424502963234	34.0	30.0	35.0	2.0	35.0
100-101	27.40520595166756	33.0	27.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69362.0
3	20617.0
4	10995.0
5	7389.0
6	6467.0
7	6289.0
8	6499.0
9	7024.0
10	7475.0
11	8123.0
12	8401.0
13	8737.0
14	9477.0
15	10245.0
16	10895.0
17	11538.0
18	10716.0
19	9692.0
20	9159.0
21	9008.0
22	9598.0
23	10727.0
24	12590.0
25	14939.0
26	18444.0
27	21853.0
28	26190.0
29	31675.0
30	38327.0
31	47473.0
32	60508.0
33	79985.0
34	115918.0
35	174604.0
36	303612.0
37	556049.0
38	512127.0
39	50197.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.07585296981959	20.249333766544243	14.208530964350663	26.466282299285503
2	15.55400671175174	21.356127056058003	35.6492691786227	27.44059705356756
3	18.050547861174497	24.26904332520719	28.217518954697766	29.462889858920548
4	13.955380470543352	17.532701626990335	32.9524805654922	35.559437336974106
5	15.321262936931616	36.42530590090007	33.01774630387164	15.23568485829668
6	34.756740537528394	33.92652921256805	16.41021904067898	14.906511209224572
7	29.687881093477074	31.023200488656943	19.989498162178382	19.2994202556876
8	27.200098075525013	34.17529114028229	19.04405511433	19.580555669862694
9	26.61734662822567	15.611853955076157	19.031808124218255	38.738991292479916
10-11	26.23594217336193	25.211922534288256	26.70767721919366	21.84445807315615
12-13	26.67076881663047	22.595366413722186	26.59562703098216	24.138237738665186
14-15	24.126422775989877	24.346586677211903	23.95656306832004	27.570427478478187
16-17	24.285102545220113	27.10154904450299	24.876121019048213	23.737227391228682
18-19	24.16895475006542	26.429961929692848	26.22862630766886	23.172457012572867
20-21	24.804573682039084	25.430915218637644	26.18008605123424	23.58442504808903
22-23	24.662874136891563	25.24506310654954	25.803933654804634	24.28812910175426
24-25	24.355425340617558	25.62704279446782	25.788473120307604	24.229058744607023
26-27	24.129870024097077	25.91431402416481	25.9322748451415	24.023541106596618
28-29	24.157330102830805	26.022692394087017	25.88570897013157	23.93426853295061
30-31	24.596766068801955	25.57422492442914	25.81591020068345	24.013098806085452
32-33	24.37647769972927	25.560909630690283	25.642706942268163	24.419905727312287
34-35	24.574505002967634	25.820225831779137	25.653356642861997	23.95191252239123
36-37	24.451389024329867	25.80617506556713	25.82900059132043	23.91343531878258
38-39	24.3951357097543	25.860681034926188	25.868203796101024	23.87597945921849
40-41	24.55714917386167	25.616107430883297	25.85607538371617	23.97066801153887
42-43	24.437128251294087	25.552797693684425	25.63375451456987	24.376319540451618
44-45	24.4230046241937	25.682708379148732	25.573630221401437	24.32065677525613
46-47	24.437116257037186	25.7391925013852	25.465753703598516	24.357937537979097
48-49	24.175788608169064	25.848839990843047	25.717873289394827	24.257498111593062
50-51	24.27681413486152	25.76021241558643	25.790755300905293	24.172218148646756
52-53	24.536302767394808	25.605303316072835	25.56466684955934	24.293727066973013
54-55	24.140924938322513	25.588671446265522	25.73338394269968	24.537019672712283
56-57	24.221791551247133	25.753731362477783	25.54691927362639	24.477557812648694
58-59	24.20480899897544	25.752622486292765	25.75017897638369	24.292389538348104
60-61	24.308567472476124	25.67952596669807	25.896330400096883	24.115576160728928
62-63	24.200528568712325	25.62998775361727	25.86599803517689	24.30348564249351
64-65	24.104206454224332	25.45127344482282	25.806563649944387	24.637956451008456
66-67	24.096369010329234	25.734235688659112	25.55607153837347	24.613323762638185
68-69	24.034924716946264	25.65956547333976	25.704649125703	24.600860684010975
70-71	23.944515067513343	25.48157273917618	25.795828124152425	24.778084069158055
72-73	23.980479853127274	25.63684972235198	25.659080429067643	24.723589995453104
74-75	23.917812812046733	25.62529395901102	25.634726372126703	24.82216685681554
76-77	23.96111268865965	25.422812841121008	25.677666881604672	24.938407588614673
78-79	23.829044483485113	25.454005023435837	25.74429373374418	24.972656759334875
80-81	23.91359307845726	25.57741886994424	25.64314247612668	24.86584557547182
82-83	23.761965754709397	25.55112409228543	25.800262600819956	24.886647552185217
84-85	23.805059371311135	25.50018501946885	25.75012209569816	24.944633513521858
86-87	23.655990547229855	25.64527188832553	25.63793651123889	25.060801053205722
88-89	23.591582432117118	25.480129412457302	25.688368477435542	25.23991967799004
90-91	23.579119200076352	25.421750095226958	25.777808636375752	25.221322068320934
92-93	23.511054799375863	25.32003951351292	25.883945459001513	25.2849602281097
94-95	23.386620748012813	25.242978351103663	25.955506230439457	25.414894670444067
96-97	23.31836008291624	25.224786305916858	25.896171221908542	25.560682389258353
98-99	23.190156121036402	25.21917449150768	25.747315092386025	25.843354295069894
100-101	23.14821621656377	25.242354716301758	25.807361462219113	25.802067604915358
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	17.0
2	27.5
3	43.5
4	42.5
5	41.5
6	65.0
7	191.5
8	290.5
9	303.0
10	308.0
11	251.0
12	205.5
13	246.0
14	288.0
15	314.5
16	374.5
17	415.5
18	460.0
19	527.5
20	598.0
21	681.5
22	803.0
23	961.0
24	1183.5
25	1480.0
26	1930.0
27	2684.5
28	3770.0
29	5380.5
30	7582.5
31	10909.5
32	15566.5
33	19759.0
34	23793.5
35	28772.0
36	34868.0
37	41256.0
38	47019.5
39	52843.5
40	58799.5
41	64710.5
42	70695.5
43	76630.0
44	82393.0
45	88282.5
46	93635.5
47	98510.5
48	103091.5
49	105571.5
50	106089.0
51	106176.0
52	105515.5
53	104696.5
54	102646.0
55	97766.0
56	91030.5
57	82600.0
58	74196.0
59	65139.0
60	54549.5
61	44981.5
62	36810.0
63	28522.5
64	20875.0
65	15481.5
66	11745.0
67	9123.5
68	7024.0
69	5288.0
70	3919.0
71	2878.0
72	2081.5
73	1547.5
74	1198.0
75	862.5
76	616.5
77	404.5
78	230.0
79	127.0
80	75.5
81	37.0
82	22.5
83	17.5
84	14.0
85	11.0
86	6.0
87	6.0
88	9.0
89	8.0
90	5.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13017986854276561
2	1.285938773883098E-4
3	0.005443807476105115
4	0.003900680947445397
5	0.023704138065245104
6	0.0011144802706986848
7	4.286462579610326E-5
8	0.001285938773883098
9	0.0
10-11	0.08840829070446299
12-13	1.285938773883098E-4
14-15	0.005079458156838237
16-17	1.7145850318441304E-4
18-19	2.3575544187856795E-4
20-21	5.786724482473941E-4
22-23	2.786200676746712E-4
24-25	0.0013288033996792011
26-27	0.00302195611862528
28-29	0.006944069378968728
30-31	0.014874025151247831
32-33	0.01418819113851018
34-35	0.0111662350198849
36-37	4.929431966551875E-4
38-39	2.571877547766196E-4
40-41	0.0049079996536538235
42-43	0.008615789785016755
44-45	0.008358602030240136
46-47	0.009794566994409596
48-49	0.011659178216540087
50-51	0.0060224799243525085
52-53	0.00197177278662075
54-55	0.0010501833320045299
56-57	0.005658130605085631
58-59	0.009258759171958305
60-61	0.007651335704604432
62-63	0.017167282631339358
64-65	0.032062740095485244
66-67	0.03459175301745533
68-69	0.025504452348681438
70-71	0.0388139186583715
72-73	0.0242828105134925
74-75	0.023275491807284072
76-77	0.219595477953437
78-79	0.06232516590753414
80-81	0.05107320163605704
82-83	0.020939369701396444
84-85	0.03167695846332031
86-87	0.07533457983665148
88-89	0.2483147772368262
90-91	0.2959373764962969
92-93	0.04567225878574803
94-95	0.0045007857085908425
96-97	0.009023003730079738
98-99	0.0010501833320045299
100-101	0.0014359649641694592
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2332926.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.71679749834121
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.26419581852907	56.529320854257506
2	10.58135850598454	14.542341394261799
3	3.0911581518823485	6.372434662747381
4	1.379062536136414	3.7905904413293925
5	0.7336680664058399	2.520765997510483
6	0.4545975858536798	1.874309415020526
7	0.31994651922291495	1.5389990120218129
8	0.21700089953936688	1.1929285496483648
9	0.17317259209332136	1.0709879348825648
>10	0.7593538140331882	8.678608923592908
>50	0.01977867001897953	0.9084152098671426
>100	0.0063940573967311435	0.8157438324261632
>500	1.8766974218674556E-4	0.07698950011232737
>1k	1.2511316145717304E-4	0.0875642723216408
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.286462579610326E-5	0.0
3	0.0	0.0	0.0	4.286462579610326E-5	0.0
4	0.0	0.0	0.0	4.286462579610326E-5	0.0
5	0.0	0.0	0.0	4.286462579610326E-5	0.0
6	0.0	4.286462579610326E-5	0.0	4.286462579610326E-5	4.286462579610326E-5
7	0.0	4.286462579610326E-5	0.0	4.286462579610326E-5	4.286462579610326E-5
8	0.0	4.286462579610326E-5	0.0	4.286462579610326E-5	8.572925159220652E-5
9	0.0	4.286462579610326E-5	0.0	4.286462579610326E-5	8.572925159220652E-5
10-11	0.0	4.286462579610326E-5	0.0	6.429693869415489E-5	8.572925159220652E-5
12-13	0.0	4.286462579610326E-5	0.0	8.572925159220652E-5	1.0716156449025815E-4
14-15	0.0	4.286462579610326E-5	0.0	8.572925159220652E-5	1.7145850318441304E-4
16-17	0.0	4.286462579610326E-5	0.0	1.0716156449025815E-4	1.7145850318441304E-4
18-19	0.0	4.286462579610326E-5	0.0	1.285938773883098E-4	2.571877547766196E-4
20-21	0.0	6.429693869415489E-5	0.0	1.285938773883098E-4	2.571877547766196E-4
22-23	0.0	8.572925159220652E-5	0.0	1.285938773883098E-4	3.0005238057272284E-4
24-25	0.0	8.572925159220652E-5	0.0	2.143231289805163E-4	3.8578163216492937E-4
26-27	0.0	8.572925159220652E-5	0.0	3.857816321649293E-4	3.8578163216492937E-4
28-29	0.0	8.572925159220652E-5	0.0	0.0017360173447421821	4.07213945062981E-4
30-31	0.0	8.572925159220652E-5	0.0	0.007008366317662883	4.286462579610326E-4
32-33	0.0	8.572925159220652E-5	0.0	0.021603771401236045	4.286462579610326E-4
34-35	0.0	8.572925159220652E-5	0.0	0.04541507103097141	5.143755095532392E-4
36-37	0.0	8.572925159220652E-5	0.0	0.07649192473314628	5.143755095532392E-4
38-39	0.0	8.572925159220652E-5	0.0	0.11854212263912356	6.001047611454457E-4
40-41	0.0	8.572925159220652E-5	0.0	0.16745066067247738	6.001047611454457E-4
42-43	0.0	8.572925159220652E-5	0.0	0.22701105821616288	6.001047611454457E-4
44-45	0.0	8.572925159220652E-5	0.0	0.3089682227383123	6.001047611454457E-4
46-47	0.0	8.572925159220652E-5	0.0	0.4031632379252492	6.001047611454457E-4
48-49	0.0	8.572925159220652E-5	0.0	0.48891392183035376	6.858340127376522E-4
50-51	0.0	8.572925159220652E-5	0.0	0.5738073132195363	7.286986385337554E-4
52-53	0.0	8.572925159220652E-5	0.0	0.672010170918409	8.14427890125962E-4
54-55	2.143231289805163E-5	8.572925159220652E-5	0.0	0.8023400656514609	9.001571417181685E-4
56-57	4.286462579610326E-5	8.572925159220652E-5	0.0	0.9630181154481539	9.215894546162201E-4
58-59	4.286462579610326E-5	8.572925159220652E-5	0.0	1.1137944366859471	0.0010073187062084267
60-61	4.286462579610326E-5	8.572925159220652E-5	0.0	1.2585911426251841	0.0010716156449025816
62-63	4.286462579610326E-5	8.572925159220652E-5	0.0	1.4217553407180512	0.0010716156449025816
64-65	4.286462579610326E-5	8.572925159220652E-5	0.0	1.6270340336555895	0.0010716156449025816
66-67	4.286462579610326E-5	8.572925159220652E-5	0.0	1.8926875520269395	0.0010716156449025816
68-69	4.286462579610326E-5	8.572925159220652E-5	0.0	2.165606624470729	0.0010716156449025816
70-71	4.286462579610326E-5	8.572925159220652E-5	0.0	2.4204153925156646	0.0010716156449025816
72-73	4.286462579610326E-5	8.572925159220652E-5	0.0	2.6773030949117116	0.0011573448964947882
74-75	4.286462579610326E-5	8.572925159220652E-5	0.0	2.9872786363562325	0.0011573448964947882
76-77	4.286462579610326E-5	8.572925159220652E-5	0.0	3.383497804902513	0.0011573448964947882
78-79	4.286462579610326E-5	8.572925159220652E-5	0.0	3.8183594336039803	0.0011573448964947882
80-81	4.286462579610326E-5	8.572925159220652E-5	0.0	4.228316714717913	0.0012002095222908914
82-83	4.286462579610326E-5	8.572925159220652E-5	0.0	4.6240643723804356	0.001285938773883098
84-85	4.286462579610326E-5	8.572925159220652E-5	0.0	5.072021144262613	0.001285938773883098
86-87	4.286462579610326E-5	1.0716156449025815E-4	0.0	5.615887516363571	0.001285938773883098
88-89	4.286462579610326E-5	1.285938773883098E-4	0.0	6.216142303699303	0.001285938773883098
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2410	0.0	46.740055	1
GTATCAA	3855	0.0	38.960148	1
TCAACGC	4975	0.0	30.05175	4
CAACGCA	5135	0.0	29.255474	5
ATCAACG	5165	0.0	29.038157	3
TATCAAC	5115	0.0	28.765265	2
AACGCAG	5575	0.0	27.115324	6
GTACATG	7110	0.0	25.067995	1
TACATGG	7430	0.0	23.63552	2
ACATGGG	7750	0.0	22.047182	3
ACGCAGA	6930	0.0	21.127592	7
GTGGTAT	1340	0.0	20.926912	1
CGCAGAG	6985	0.0	20.416784	8
CATGGGG	6735	0.0	19.94349	4
ACGATAG	120	0.0047481935	19.803717	1
TGGTATC	1370	0.0	18.707918	2
GTAAGTA	1145	0.0	17.409893	2
GCAGAGT	7735	0.0	17.207994	9
TAAGTAC	1575	0.0	16.875622	3
ATGGGGG	4115	0.0	16.405106	5
>>END_MODULE