FastQCFastQC Report
Mon 15 Aug 2016
SRR2921385_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921385_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3989650
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34700.054.0161251
GTATCAA68350.044.9875451
TCAACGC80900.037.1883964
CAACGCA83550.035.666755
ATCAACG86450.034.9102823
TATCAAC87100.034.651932
AACGCAG87950.034.084966
GTACATG114050.031.1632211
TACATGG118800.029.520012
ACGCAGA105750.027.591097
ACATGGG125700.026.9540583
CGCAGAG107500.026.6601458
CATGGGG107500.023.8370234
GCAGAGT123800.022.1534429
ATGGGGG70400.022.1081075
AGAGTAC103250.019.37077510-11
GAGTACA87050.018.9699541
CAGAGTA116450.018.25483910-11
GAGTACT58350.017.76869412-13
AGTACAT90850.017.0809232
Produced by FastQC (version 0.11.5)