##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921385_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3989650
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.204012131389973	31.0	31.0	34.0	26.0	34.0
2	30.255104081811687	31.0	31.0	34.0	26.0	34.0
3	30.304661812439686	31.0	31.0	34.0	26.0	34.0
4	33.76840224079807	37.0	35.0	37.0	28.0	37.0
5	33.76003609339165	37.0	35.0	37.0	28.0	37.0
6	33.80289925181407	37.0	35.0	37.0	29.0	37.0
7	33.71347486621634	37.0	35.0	37.0	28.0	37.0
8	33.681776596944594	37.0	35.0	37.0	28.0	37.0
9	35.21261288584212	39.0	35.0	39.0	28.0	39.0
10-11	35.10250473099144	39.0	35.0	39.0	28.0	39.0
12-13	34.98204366297796	39.0	35.0	39.0	27.0	39.0
14-15	36.08677916609226	40.0	36.0	41.0	27.0	41.0
16-17	36.03135124634993	40.0	36.0	41.0	27.0	41.0
18-19	35.98891406514356	40.0	36.0	41.0	27.0	41.0
20-21	35.92913137743912	40.0	36.0	41.0	27.0	41.0
22-23	35.811712806887826	40.0	36.0	41.0	27.0	41.0
24-25	35.711556402190666	40.0	36.0	41.0	27.0	41.0
26-27	35.55909327885905	40.0	36.0	41.0	25.5	41.0
28-29	35.41172057699297	40.0	35.5	41.0	25.0	41.0
30-31	35.25399207950572	39.0	35.0	41.0	24.5	41.0
32-33	35.06527602671913	39.0	35.0	41.0	24.0	41.0
34-35	34.895152456982444	39.0	35.0	41.0	24.0	41.0
36-37	34.716167834271175	39.0	35.0	41.0	23.0	41.0
38-39	34.93218064241224	39.0	35.0	41.0	24.0	41.0
40-41	35.0441542491196	40.0	35.0	41.0	23.5	41.0
42-43	34.867767222688705	40.0	35.0	41.0	22.0	41.0
44-45	34.73429887333475	40.0	35.0	41.0	20.5	41.0
46-47	34.56451267655058	39.0	35.0	41.0	18.5	41.0
48-49	34.40386662990488	39.0	35.0	41.0	17.5	41.0
50-51	32.6934744902435	37.0	32.0	39.5	13.5	40.0
52-53	32.84728986251927	37.5	32.5	39.5	13.0	40.0
54-55	33.70019450327723	38.5	34.0	40.0	11.5	41.0
56-57	33.71668918326169	38.5	34.0	41.0	10.0	41.0
58-59	33.53738410637524	38.0	34.0	40.5	8.0	41.0
60-61	33.292251074655674	37.5	33.0	40.0	7.5	41.0
62-63	33.00076648327547	37.0	33.0	40.0	4.0	41.0
64-65	32.71377526850726	37.0	33.0	40.0	2.0	41.0
66-67	32.37803491534345	36.0	32.5	39.0	2.0	41.0
68-69	32.01595340443397	35.5	32.0	39.0	2.0	41.0
70-71	31.63300652939481	35.0	32.0	39.0	2.0	40.5
72-73	31.277031318536714	35.0	32.0	37.5	2.0	40.0
74-75	30.88598009850488	35.0	31.0	37.0	2.0	39.0
76-77	30.514363039364355	35.0	31.0	36.5	2.0	39.0
78-79	30.17325391951675	35.0	31.0	36.0	2.0	38.5
80-81	29.82958292080759	35.0	31.0	36.0	2.0	37.0
82-83	29.528366147406416	35.0	30.0	35.0	2.0	37.0
84-85	29.294061133182108	35.0	30.0	35.0	2.0	36.5
86-87	29.06868898274285	35.0	30.0	35.0	2.0	36.0
88-89	28.86690311180179	34.5	30.0	35.0	2.0	36.0
90-91	28.61255486070207	34.0	30.0	35.0	2.0	35.5
92-93	28.415150326469742	34.0	29.5	35.0	2.0	35.0
94-95	28.200793929292043	34.0	29.0	35.0	2.0	35.0
96-97	27.947035328913564	34.0	29.0	35.0	2.0	35.0
98-99	27.71489165716291	34.0	29.0	35.0	2.0	35.0
100-101	26.66630230721993	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	150610.0
3	25187.0
4	17212.0
5	13064.0
6	11839.0
7	12130.0
8	13037.0
9	13691.0
10	15042.0
11	16143.0
12	16392.0
13	16644.0
14	17949.0
15	19462.0
16	20539.0
17	22432.0
18	20352.0
19	18056.0
20	17885.0
21	18659.0
22	20504.0
23	23176.0
24	27243.0
25	31851.0
26	37592.0
27	44316.0
28	52262.0
29	61650.0
30	73293.0
31	88540.0
32	110698.0
33	143341.0
34	198810.0
35	286306.0
36	465564.0
37	828666.0
38	860145.0
39	159346.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.53310930270467	17.87756893330491	12.651527880528143	24.937793883462277
2	16.00927463245068	19.437302180466588	39.425890720853126	25.127532466229603
3	19.497977852804937	23.566146294268247	29.130179534370736	27.805696318556084
4	13.388389624792435	15.258119941925802	36.37770598897052	34.975784444311245
5	14.205412058438958	37.301654573386486	34.20421334662783	14.28872002154672
6	33.83233435358042	34.86046163569282	16.934806386618074	14.372397624108684
7	29.209939444016047	31.86772443266995	20.66301231676024	18.259323806553766
8	26.956333603089462	34.90912527815467	19.63358293296109	18.500958185794783
9	27.195857183542653	15.57838178251818	19.23177589962254	37.993985134316624
10-11	25.923082756766348	25.195126063376723	27.51901433212139	21.36277684773555
12-13	26.30585440219369	22.628796559934322	27.537561785004165	23.527787252867817
14-15	23.466328877385173	24.23441666715774	24.79915133075996	27.500103124697127
16-17	23.59235889490528	27.36750538335087	25.672738651023845	23.36739707072
18-19	23.626727304749615	26.69208017414228	27.069998502015384	22.61119401909272
20-21	23.971542085463753	25.90460985070664	27.231104786907245	22.892743276922364
22-23	23.904484675348904	25.617151291527723	26.933755759501953	23.544608273621417
24-25	23.688027310436553	25.85946930810652	26.872314080050636	23.580189301406296
26-27	23.421568271025635	26.094240680171648	26.92131315651244	23.562877892290278
28-29	23.490926430203483	26.39436597826234	26.70060804675447	23.414099544779706
30-31	23.95683839176646	26.02187886282456	26.599947307030803	23.42133543837818
32-33	23.595690242307306	26.097486935589515	26.547970018597372	23.7588528035058
34-35	23.987362143181585	26.152011238106887	26.642663194698596	23.21796342401293
36-37	23.98815822915847	25.96127286754632	26.699227943811994	23.351340959483217
38-39	23.81048528600792	26.185692084022662	26.58756955933223	23.41625307063719
40-41	23.939097898430074	25.999973681027843	26.6444125135433	23.41651590699878
42-43	23.71341712407495	25.999577206905215	26.44671293441645	23.84029273460338
44-45	23.57347498092843	26.11468055516314	26.66385380662215	23.64799065728628
46-47	23.849736781297405	26.09284738338507	26.34006701025749	23.71734882506003
48-49	23.618188517234547	26.251536141423713	26.326808013026024	23.803467328315712
50-51	23.723810270096227	26.26850441202546	26.435547056498137	23.572138261380175
52-53	23.97300916468235	26.086837490742955	26.39025896629235	23.549894378282353
54-55	23.379253729412707	26.019909875482593	26.748468507342032	23.852367887762664
56-57	23.519969214078582	26.20318733255791	26.472250156711542	23.80459329665197
58-59	23.621241779746825	26.103223848850575	26.57745594074912	23.698078430653478
60-61	23.719816908208436	26.127581479213273	26.658396454719146	23.494205157859145
62-63	23.6808022958623	26.054846994844354	26.728383665216054	23.535967044077292
64-65	23.393304959543677	25.915660088748577	26.762427683447576	23.92860726826017
66-67	23.41230566946903	26.068933298923824	26.585126175527428	23.933634856079713
68-69	23.487872197986025	25.982313096197736	26.68391462736657	23.845900078449674
70-71	23.4221821996071	25.894739406151334	26.59822113091957	24.084857263321993
72-73	23.410559154086318	26.13558396653634	26.395599858558988	24.05825702081835
74-75	23.29648613241762	26.22175198263038	26.5510550317192	23.930706853232802
76-77	23.336682599328423	25.825666059010533	26.588567911286056	24.249083430374988
78-79	23.252757471961814	25.848323687399134	26.675530735715636	24.223388104923416
80-81	23.44159294369755	25.98454235653068	26.419280728441137	24.15458397133063
82-83	23.431742173736918	26.030039535621313	26.524639892980623	24.01357839766115
84-85	23.33254244751987	26.114844407555974	26.440120795770966	24.112492349153186
86-87	23.18045097763579	26.228575685478194	26.43389785624006	24.15707548064596
88-89	23.071802471691697	25.95968467005691	26.565704772070692	24.402808086180706
90-91	23.241158527316905	25.772243090846647	26.611248053171316	24.37535032866513
92-93	23.33181575721395	25.624159711318832	26.72722900902028	24.316795522446935
94-95	23.23614547535141	25.54448850075114	26.74796736731397	24.471398656583485
96-97	23.125396081127466	25.362673364803722	26.82345574219007	24.68847481187874
98-99	22.864318041199876	25.376637489439354	26.803564081545485	24.95548038781529
100-101	23.049295071246707	25.276207814365936	26.91970351719966	24.754793597187696
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	117.0
1	98.5
2	159.0
3	247.0
4	216.5
5	246.0
6	435.0
7	797.0
8	1193.5
9	1333.0
10	1160.0
11	1003.5
12	1059.0
13	1177.5
14	1296.5
15	1458.0
16	1641.0
17	1827.0
18	2071.0
19	2348.5
20	2655.5
21	2913.0
22	3244.0
23	3811.5
24	4546.0
25	5698.0
26	7516.5
27	10323.0
28	14084.0
29	18760.5
30	25148.5
31	33023.5
32	43558.0
33	51693.0
34	57186.5
35	64508.0
36	74551.0
37	84938.0
38	93765.0
39	102292.5
40	109196.5
41	115892.5
42	122344.0
43	129773.5
44	137755.5
45	147508.5
46	154734.0
47	159174.5
48	164878.0
49	168906.0
50	169716.0
51	168342.5
52	167191.0
53	165668.0
54	162465.5
55	154577.0
56	143156.5
57	129665.5
58	116287.0
59	101518.0
60	84136.5
61	69232.5
62	57184.5
63	44186.5
64	31548.5
65	22987.0
66	17004.0
67	12729.0
68	9628.5
69	7285.5
70	5546.5
71	4075.5
72	2897.0
73	2175.5
74	1674.5
75	1245.0
76	908.0
77	584.0
78	396.0
79	282.0
80	200.5
81	149.5
82	112.0
83	97.5
84	84.0
85	65.5
86	61.0
87	64.0
88	53.5
89	42.5
90	44.5
91	40.0
92	29.0
93	23.0
94	22.0
95	16.5
96	11.5
97	11.0
98	8.5
99	8.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05263619615755768
2	0.06090759841088818
3	0.0515584073790934
4	0.05857656686676776
5	0.051032045417517824
6	0.01872344691890266
7	0.015389821162257341
8	0.021605905279911772
9	0.021706164701164262
10-11	0.02211973481383079
12-13	0.028110235233667114
14-15	0.0441016129234394
16-17	0.029965034526838196
18-19	0.02399959896231499
20-21	0.027408419284899677
22-23	0.04742270625242816
24-25	0.043211810559823545
26-27	0.03120574486483777
28-29	0.020352662514255635
30-31	0.01260762222250072
32-33	0.00944945045304726
34-35	0.00917373704460291
36-37	0.001102853633777399
38-39	0.006266213828280676
40-41	0.003321093328988758
42-43	0.22506986828418532
44-45	0.19895228904791146
46-47	0.2047798679082125
48-49	0.2025992254959708
50-51	0.2013209178750016
52-53	0.20614590252277767
54-55	0.20449162207211158
56-57	0.21579587181832996
58-59	0.21247477848934118
60-61	0.21796398180291507
62-63	0.2149311343100272
64-65	0.21627210406927927
66-67	0.20534382715275776
68-69	0.20343889814896043
70-71	0.186908626069956
72-73	0.1949544446254684
74-75	0.19439048538092313
76-77	0.19257328337072174
78-79	0.2201822214981264
80-81	0.227964859072851
82-83	0.2271502512751745
84-85	0.2226135124634993
86-87	0.23970774378704898
88-89	0.2381537227576354
90-91	0.23708846640682768
92-93	0.24109884325692732
94-95	0.25116238266514607
96-97	0.25155088792249947
98-99	0.2421891644630481
100-101	0.2504605667163787
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3989650.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.91618660124023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.37243020244514	41.177101979852274
2	12.520318517937945	13.500956590402117
3	4.3276746867135865	6.999951478749408
4	2.072459392593438	4.469564293382431
5	1.11068458941848	2.9941938789104325
6	0.7237468737640793	2.341300289875701
7	0.4980444130977978	1.8796858858600225
8	0.35435461372741434	1.528435958139014
9	0.2846413891842026	1.3812100428322542
>10	1.602541033346716	15.784946126737697
>50	0.09487592636783347	3.4755972609072963
>100	0.0359841448718992	3.1455893982488354
>500	0.0012623718502692268	0.4570759882343963
>1k	9.818446811401106E-4	0.8643908278680014
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.012971062624541E-5
2	0.0	0.0	0.0	0.0	7.519456593936812E-5
3	0.0	0.0	0.0	0.0	7.519456593936812E-5
4	0.0	0.0	0.0	5.012971062624541E-5	7.519456593936812E-5
5	0.0	0.0	0.0	5.012971062624541E-5	7.519456593936812E-5
6	0.0	0.0	0.0	5.012971062624541E-5	1.2532427656561352E-4
7	0.0	0.0	0.0	5.012971062624541E-5	1.5038913187873624E-4
8	0.0	0.0	0.0	5.012971062624541E-5	2.0051884250498164E-4
9	0.0	0.0	0.0	7.519456593936812E-5	2.0051884250498164E-4
10-11	0.0	0.0	0.0	7.519456593936812E-5	2.13051270161543E-4
12-13	0.0	1.2532427656561352E-5	0.0	8.772699359592947E-5	3.7597282969684055E-4
14-15	0.0	2.5064855313122705E-5	0.0	1.1279184890905217E-4	4.135701126665246E-4
16-17	0.0	2.5064855313122705E-5	0.0	1.5038913187873624E-4	4.511673956362087E-4
18-19	0.0	2.5064855313122705E-5	0.0	1.5038913187873624E-4	4.762322509493314E-4
20-21	0.0	3.759728296968406E-5	0.0	1.629215595352976E-4	4.887646786058928E-4
22-23	0.0	5.012971062624541E-5	0.0	2.882458361009111E-4	5.764916722018221E-4
24-25	0.0	5.012971062624541E-5	0.0	4.26102540323086E-4	7.394132317371198E-4
26-27	0.0	5.012971062624541E-5	0.0	5.639592445452608E-4	7.895429423633651E-4
28-29	0.0	5.012971062624541E-5	0.0	0.0026067449525647613	8.020753700199266E-4
30-31	0.0	5.012971062624541E-5	0.0	0.010965874199491183	8.14607797676488E-4
32-33	0.0	5.012971062624541E-5	0.0	0.0268820573233241	9.273996465855401E-4
34-35	0.0	5.012971062624541E-5	0.0	0.04774854937149875	9.273996465855401E-4
36-37	0.0	5.012971062624541E-5	0.0	0.08136052034639629	9.273996465855401E-4
38-39	0.0	5.012971062624541E-5	0.0	0.1367789154437106	9.775293572117855E-4
40-41	0.0	5.012971062624541E-5	0.0	0.20110786660484004	9.775293572117855E-4
42-43	0.0	5.012971062624541E-5	0.0	0.26974797287982655	9.775293572117855E-4
44-45	0.0	5.012971062624541E-5	0.0	0.35217374957703057	9.90061784868347E-4
46-47	1.2532427656561352E-5	5.012971062624541E-5	0.0	0.44808441843269464	0.0010025942125249081
48-49	2.5064855313122705E-5	5.012971062624541E-5	0.0	0.5501359768400736	0.0010527239231511536
50-51	2.5064855313122705E-5	5.012971062624541E-5	0.0	0.6636296416978933	0.0010527239231511536
52-53	2.5064855313122705E-5	5.012971062624541E-5	0.0	0.7841038687604176	0.001140450916747083
54-55	2.5064855313122705E-5	5.012971062624541E-5	0.0	0.9224493376611984	0.0012281779103430126
56-57	2.5064855313122705E-5	5.012971062624541E-5	0.0	1.083541162758638	0.001240710337999574
58-59	2.5064855313122705E-5	5.012971062624541E-5	0.0	1.2570024939531037	0.001278307620969258
60-61	2.5064855313122705E-5	5.012971062624541E-5	0.0	1.4432844986402316	0.0013284373315955035
62-63	2.5064855313122705E-5	5.012971062624541E-5	0.0	1.6382514756933566	0.001353502186908626
64-65	2.5064855313122705E-5	5.012971062624541E-5	0.0	1.8546489040392014	0.0013785670422217487
66-67	2.5064855313122705E-5	5.012971062624541E-5	0.0	2.109257704309902	0.0013910994698783101
68-69	2.5064855313122705E-5	5.012971062624541E-5	0.0	2.387590390134473	0.0014036318975348715
70-71	2.5064855313122705E-5	6.266213828280676E-5	0.0	2.6772649229882317	0.0014788264634742396
72-73	2.5064855313122705E-5	7.519456593936812E-5	0.0	2.983971025027258	0.0015790858847267303
74-75	2.5064855313122705E-5	7.519456593936812E-5	0.0	3.3071447370070057	0.0016166831676964143
76-77	2.5064855313122705E-5	7.519456593936812E-5	0.0	3.6745453861867583	0.0016292155953529757
78-79	2.5064855313122705E-5	7.519456593936812E-5	0.0	4.078039427017408	0.0016417480230095371
80-81	5.012971062624541E-5	7.519456593936812E-5	0.0	4.508327798177785	0.001742007444262028
82-83	5.012971062624541E-5	8.772699359592947E-5	0.0	4.95601117892547	0.0019049290037973256
84-85	5.012971062624541E-5	1.0025942125249082E-4	0.0	5.419924053488401	0.0019049290037973256
86-87	5.012971062624541E-5	1.0025942125249082E-4	0.0	5.92655997393255	0.0019299938591104482
88-89	5.012971062624541E-5	1.0025942125249082E-4	0.0	6.482924567317935	0.0019299938591104482
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3470	0.0	54.016125	1
GTATCAA	6835	0.0	44.987545	1
TCAACGC	8090	0.0	37.188396	4
CAACGCA	8355	0.0	35.66675	5
ATCAACG	8645	0.0	34.910282	3
TATCAAC	8710	0.0	34.65193	2
AACGCAG	8795	0.0	34.08496	6
GTACATG	11405	0.0	31.163221	1
TACATGG	11880	0.0	29.52001	2
ACGCAGA	10575	0.0	27.59109	7
ACATGGG	12570	0.0	26.954058	3
CGCAGAG	10750	0.0	26.660145	8
CATGGGG	10750	0.0	23.837023	4
GCAGAGT	12380	0.0	22.153442	9
ATGGGGG	7040	0.0	22.108107	5
AGAGTAC	10325	0.0	19.370775	10-11
GAGTACA	8705	0.0	18.969954	1
CAGAGTA	11645	0.0	18.254839	10-11
GAGTACT	5835	0.0	17.768694	12-13
AGTACAT	9085	0.0	17.080923	2
>>END_MODULE