FastQCFastQC Report
Mon 15 Aug 2016
SRR2921385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3989650
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33550.052.2817961
GTATCAA66250.046.42321
TCAACGC85350.034.6438034
CAACGCA86500.034.084425
TATCAAC89050.033.3109552
ATCAACG90000.032.853873
AACGCAG92050.032.1811036
GTACATG120300.028.8057081
TACATGG124500.027.2188552
ACATGGG130400.025.5869623
ACGCAGA114500.025.2481337
CGCAGAG118250.024.3670358
CATGGGG105700.023.8878824
ATGGGGG64450.021.101995
GCAGAGT131150.021.1001669
AGAGTAC112600.019.38230710-11
CGTATAC2451.7738239E-719.372023
GAGTACA90900.019.0350571
ACCGTAT3651.1641532E-1018.2375458
TATACCG4253.6379788E-1217.9024035
Produced by FastQC (version 0.11.5)