##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921385_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3989650
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43808053338012	33.0	31.0	34.0	29.0	34.0
2	31.555292820172195	34.0	31.0	34.0	29.0	34.0
3	31.632040153898213	34.0	31.0	34.0	28.0	34.0
4	35.13290012908401	37.0	35.0	37.0	33.0	37.0
5	34.881719699723035	37.0	35.0	37.0	32.0	37.0
6	34.8437745165616	37.0	35.0	37.0	32.0	37.0
7	34.708802275888864	37.0	35.0	37.0	32.0	37.0
8	34.67214317045355	37.0	35.0	37.0	32.0	37.0
9	36.29291917837404	39.0	37.0	39.0	32.0	39.0
10-11	36.14569410850576	39.0	37.0	39.0	32.0	39.0
12-13	36.07899439800484	39.0	37.0	39.0	32.0	39.0
14-15	37.31716115448724	40.0	38.0	41.0	32.0	41.0
16-17	37.29855651498252	40.0	38.0	41.0	31.5	41.0
18-19	37.26679545824822	40.0	38.0	41.0	31.0	41.0
20-21	37.23392565763915	40.0	38.0	41.0	31.0	41.0
22-23	37.126127730502674	40.0	38.0	41.0	31.0	41.0
24-25	37.05967465817804	40.0	37.0	41.0	31.0	41.0
26-27	36.923729525146314	40.0	37.0	41.0	30.0	41.0
28-29	36.765868559898735	40.0	37.0	41.0	30.0	41.0
30-31	36.62166017069167	40.0	37.0	41.0	30.0	41.0
32-33	36.5584635243693	40.0	36.5	41.0	30.0	41.0
34-35	36.68075257228078	40.0	37.0	41.0	30.0	41.0
36-37	36.65023774015265	40.0	37.0	41.0	30.0	41.0
38-39	36.55741242966175	40.0	37.0	41.0	30.0	41.0
40-41	36.42810609953254	40.0	37.0	41.0	30.0	41.0
42-43	36.31384745529057	40.0	36.0	41.0	29.5	41.0
44-45	36.16246249671023	40.0	36.0	41.0	29.0	41.0
46-47	35.986610855588836	40.0	36.0	41.0	28.0	41.0
48-49	35.813240259170605	40.0	35.5	41.0	27.5	41.0
50-51	35.65115260737157	40.0	35.0	41.0	27.0	41.0
52-53	35.47666599827052	39.0	35.0	41.0	27.0	41.0
54-55	35.26562743599062	39.0	35.0	41.0	26.0	41.0
56-57	35.00064416678155	39.0	35.0	41.0	26.0	41.0
58-59	34.77335894126051	38.5	35.0	40.5	26.0	41.0
60-61	34.49975398844511	38.0	34.0	40.0	25.5	41.0
62-63	34.20063100773251	37.5	34.0	40.0	24.5	41.0
64-65	33.887376336270094	37.0	34.0	40.0	23.5	41.0
66-67	33.5535461757297	36.5	34.0	39.0	23.0	41.0
68-69	33.18953078590854	36.0	34.0	39.0	22.0	41.0
70-71	32.80730903713358	35.0	33.0	39.0	22.0	40.0
72-73	32.414360156906	35.0	33.0	37.5	20.5	40.0
74-75	32.011759427518705	35.0	33.0	37.0	19.5	39.0
76-77	29.260213301918714	33.0	29.0	34.5	12.5	36.5
78-79	30.918362513002393	34.5	31.5	36.0	15.5	37.5
80-81	31.113738423169952	35.0	32.0	36.0	17.5	37.0
82-83	30.953308811549885	35.0	32.0	35.0	16.0	37.0
84-85	30.72279849109571	35.0	32.0	35.0	11.0	36.5
86-87	30.480234105748625	35.0	32.0	35.0	7.0	36.0
88-89	30.20008033286128	35.0	32.0	35.0	2.0	36.0
90-91	30.012658253230235	34.5	31.0	35.0	2.0	35.0
92-93	29.86434536864136	34.0	31.0	35.0	2.0	35.0
94-95	29.761748023009538	34.0	31.0	35.0	2.0	35.0
96-97	29.570001378567042	34.0	31.0	35.0	2.0	35.0
98-99	29.308027521211134	34.0	31.0	35.0	2.0	35.0
100-101	28.23171042572657	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70815.0
3	23889.0
4	13170.0
5	9258.0
6	8118.0
7	8347.0
8	8815.0
9	9500.0
10	10601.0
11	11588.0
12	12775.0
13	13893.0
14	15407.0
15	17136.0
16	19226.0
17	21580.0
18	19426.0
19	16194.0
20	15239.0
21	14597.0
22	15486.0
23	17532.0
24	20492.0
25	24689.0
26	29535.0
27	35738.0
28	42454.0
29	51407.0
30	62228.0
31	77761.0
32	98638.0
33	130448.0
34	188667.0
35	280776.0
36	489355.0
37	947378.0
38	1000857.0
39	136634.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.449108218309405	19.306095787139242	13.316098362845166	25.92869763170619
2	15.077884259173388	20.696213541178423	38.11105167937542	26.114850520272764
3	18.064389825227476	24.639634908583012	29.05357931115215	28.242395955037363
4	12.996273701698616	16.359241625855187	35.418633296252494	35.2258513761937
5	13.922452687183451	37.654490765592236	34.220097896280485	14.202958650943826
6	33.79942711221041	35.503778312276985	16.640465288353855	14.05632928715875
7	28.98753096454996	32.046451273961104	20.715061472918755	18.25095628857018
8	26.89012840272742	35.43245528262912	19.341119828592497	18.33629648605096
9	26.882959658115375	15.6191395235171	19.222212474778487	38.27568834358904
10-11	25.851350540961604	25.50806028900967	27.453434445650792	21.187154724377937
12-13	26.2089377008797	22.772925069160394	27.654206307889012	23.363930922070892
14-15	23.333576058766457	24.365856828507717	24.84718010908106	27.45338700364477
16-17	23.526153329431565	27.58364466011387	25.64483807456553	23.24536393588903
18-19	23.542278172111455	26.84316372786878	27.113162924538457	22.501395175481306
20-21	23.85715449447003	25.963129366795123	27.301638625184072	22.878077513550775
22-23	23.733277020887826	25.67712164866799	27.044799725288843	23.544801605155346
24-25	23.540484709380284	25.981853006570983	26.95704178866657	23.520620495382165
26-27	23.284636596733645	26.225603328491104	27.021418802169816	23.468341272605432
28-29	23.389204781876753	26.552478119494783	26.750452950772498	23.307864147855966
30-31	23.833474511581336	26.138824636561065	26.667096176104216	23.360604675753383
32-33	23.512756645804597	26.196046785983057	26.632854172589116	23.658342395623226
34-35	23.79768336787283	26.26700454958734	26.779719905453614	23.155592177086213
36-37	23.832400453477113	26.080811767144567	26.862904384054907	23.223883395323412
38-39	23.722890598397928	26.34541749271144	26.639917416200866	23.29177449268976
40-41	23.85960743411939	26.091908688132925	26.685057578443054	23.36342629930463
42-43	23.636007914615202	26.110559709484942	26.48294067535426	23.77049170054559
44-45	23.51507873035282	26.208716745319936	26.658615218615488	23.617589305711753
46-47	23.733779393992872	26.19613299900509	26.380338571270652	23.689749035731396
48-49	23.520894636973612	26.403996196223623	26.346114822457224	23.72899434434554
50-51	23.6676994388539	26.357197635899617	26.394333318001127	23.580769607245355
52-53	23.91577531578742	26.185644867562225	26.31761329890875	23.580966517741608
54-55	23.273603184271145	26.15942410411043	26.67475016354982	23.89222254806861
56-57	23.472216408237482	26.320410244789787	26.37371369243121	23.833659654541524
58-59	23.5383835797368	26.239076848376925	26.499474029279142	23.723065542607138
60-61	23.635747905609612	26.29322543263346	26.55389465995312	23.5171320018038
62-63	23.592668579943485	26.221418672699304	26.595901496326867	23.59001125103034
64-65	23.256811350736374	26.070901358039006	26.644656384475795	24.027630906748822
66-67	23.318573536397448	26.255476000003508	26.4459253971436	23.980025066455443
68-69	23.367217360455598	26.143263120815423	26.51883302558162	23.970686493147355
70-71	23.338192050643666	26.032159810592777	26.445949005785074	24.183699132978486
72-73	23.28511139508419	26.351268514764804	26.24862703863296	24.11499305151805
74-75	23.177078142888426	26.41893679882378	26.285597598916638	24.118387459371156
76-77	23.211478997303846	25.999932913384523	26.43938793388174	24.34920015542989
78-79	23.110685208721016	26.07053124174626	26.489272552196248	24.329510997336477
80-81	23.32247438554782	26.27901727776461	26.163443465132875	24.235064871554698
82-83	23.299513911355852	26.266573724990327	26.279685169890353	24.154227193763464
84-85	23.18775245149045	26.334874473158344	26.158387218902867	24.318985856448343
86-87	23.045834797159202	26.49813510361253	26.11559836539828	24.340431733829988
88-89	22.934186692185676	26.288833892566853	26.181351488821026	24.59562792642645
90-91	23.013239933452713	26.12648686963347	26.295910142804107	24.564363054109712
92-93	23.086479848185846	26.04752794164374	26.366423933550458	24.499568276619954
94-95	22.971567395883582	25.98262176937404	26.31655531579618	24.729255518946193
96-97	22.814106832019124	25.796986174727067	26.423224513287824	24.965682479965988
98-99	22.550014600516093	25.865873267520307	26.361363399952626	25.222748732010974
100-101	22.570215632984034	25.973511650156674	26.386748255402853	25.06952446145644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	94.5
2	135.0
3	194.0
4	175.5
5	182.5
6	320.5
7	683.0
8	1078.0
9	1177.0
10	980.5
11	792.0
12	791.5
13	863.0
14	962.5
15	1066.5
16	1211.0
17	1386.0
18	1496.5
19	1672.0
20	1929.5
21	2281.5
22	2737.0
23	3366.5
24	4241.0
25	5574.0
26	7717.5
27	10746.5
28	14527.5
29	19649.0
30	26320.5
31	34135.0
32	44957.5
33	52900.0
34	58029.0
35	65598.5
36	75572.0
37	85735.0
38	94278.5
39	103001.0
40	109967.0
41	116374.5
42	122726.0
43	130677.0
44	139393.0
45	148581.0
46	155956.5
47	160745.0
48	166341.5
49	170266.5
50	171369.5
51	169921.0
52	167883.5
53	166621.0
54	163189.0
55	154923.5
56	142726.0
57	128351.5
58	115050.5
59	100125.5
60	82574.0
61	67794.5
62	55809.5
63	42710.5
64	30093.0
65	21495.5
66	15769.5
67	11667.0
68	8668.5
69	6555.5
70	4946.0
71	3487.5
72	2395.0
73	1826.0
74	1442.0
75	1000.0
76	653.0
77	394.0
78	215.5
79	110.5
80	71.0
81	48.0
82	32.5
83	21.0
84	17.5
85	12.5
86	9.0
87	9.5
88	8.5
89	6.0
90	4.5
91	1.5
92	0.0
93	0.0
94	0.5
95	1.5
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1573070319451581
2	3.0077826375747243E-4
3	0.0073440026067449525
4	0.004210895692604614
5	0.02864912962289925
6	9.524645018986628E-4
7	1.7545398719185894E-4
8	0.0023310315441204114
9	0.0
10-11	0.10645044051483213
12-13	2.7571340844434976E-4
14-15	0.005802514004987907
16-17	1.2532427656561352E-4
18-19	2.13051270161543E-4
20-21	6.266213828280676E-4
22-23	1.2532427656561352E-4
24-25	0.001591618312383292
26-27	0.0023686288270900954
28-29	0.006366473249533168
30-31	0.015114107753812993
32-33	0.014838394345368641
34-35	0.012457233090621984
36-37	8.271402253330493E-4
38-39	1.3785670422217488E-4
40-41	0.0044991415287055254
42-43	0.00805835098316895
44-45	0.007657313298158987
46-47	0.008948153346784806
48-49	0.011442106450440515
50-51	0.005652124873109171
52-53	0.001491358891130801
54-55	0.0010025942125249081
56-57	0.005689722156078854
58-59	0.009010815485067613
60-61	0.00759465115987618
62-63	0.017094231323549685
64-65	0.03415086536412969
66-67	0.03638163748699761
68-69	0.026192773802213225
70-71	0.04083064930507689
72-73	0.02529043901094081
74-75	0.02382414497512313
76-77	0.24364292607120924
78-79	0.07070795683831915
80-81	0.05401476319977943
82-83	0.019262341308134798
84-85	0.03158171769453461
86-87	0.08109733936560852
88-89	0.26052410612459737
90-91	0.3165315253217701
92-93	0.05371398493602196
94-95	0.0058526437156141515
96-97	0.010878147205895253
98-99	0.001629215595352976
100-101	0.0011780481997167671
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3989650.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.63484478621928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.30983402075513	39.63921424558383
2	13.059609610845454	13.747810496709361
3	4.482561259317839	7.078167483867423
4	2.159266762165424	4.546106835144774
5	1.2072991131924753	3.1773000716713065
6	0.7388860325530748	2.333469098288188
7	0.517179762316766	1.9055173561281735
8	0.3909400755477026	1.6461656157732933
9	0.28462947342594674	1.3483285339822229
>10	1.7095619083717724	16.406085125938475
>50	0.10042244392917483	3.598595264437428
>100	0.037467672110921675	3.246293338331198
>500	0.00133820890908669	0.46309428188183666
>1k	0.0010036565592472918	0.8638522522624457
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.5064855313122705E-5
6	0.0	0.0	0.0	0.0	5.012971062624541E-5
7	0.0	0.0	0.0	0.0	5.012971062624541E-5
8	0.0	0.0	0.0	0.0	7.519456593936812E-5
9	0.0	0.0	0.0	2.5064855313122705E-5	7.519456593936812E-5
10-11	0.0	0.0	0.0	8.772699359592947E-5	7.519456593936812E-5
12-13	0.0	0.0	0.0	1.1279184890905217E-4	1.1279184890905217E-4
14-15	0.0	0.0	0.0	1.629215595352976E-4	1.629215595352976E-4
16-17	0.0	0.0	0.0	2.2558369781810434E-4	1.7545398719185894E-4
18-19	0.0	0.0	0.0	2.2558369781810434E-4	2.0051884250498164E-4
20-21	0.0	2.5064855313122705E-5	0.0	2.631809807877884E-4	2.3811612547466567E-4
22-23	0.0	2.5064855313122705E-5	0.0	3.7597282969684055E-4	2.8824583610091107E-4
24-25	0.0	2.5064855313122705E-5	0.0	5.263619615755768E-4	3.509079743837179E-4
26-27	0.0	2.5064855313122705E-5	0.0	6.892835211108743E-4	3.7597282969684055E-4
28-29	0.0	2.5064855313122705E-5	0.0	0.002844861078039427	3.7597282969684055E-4
30-31	0.0	2.5064855313122705E-5	0.0	0.011354379456844586	3.7597282969684055E-4
32-33	0.0	2.5064855313122705E-5	0.0	0.027884651535849007	4.26102540323086E-4
34-35	0.0	2.5064855313122705E-5	0.0	0.04956575138170015	5.012971062624541E-4
36-37	0.0	2.5064855313122705E-5	0.0	0.0840173950095873	5.138295339190155E-4
38-39	0.0	2.5064855313122705E-5	0.0	0.14040078703645684	5.263619615755768E-4
40-41	0.0	2.5064855313122705E-5	0.0	0.20579499454839395	5.263619615755768E-4
42-43	0.0	2.5064855313122705E-5	0.0	0.2754000977529357	5.388943892321381E-4
44-45	0.0	2.5064855313122705E-5	0.0	0.3590540523604827	5.639592445452609E-4
46-47	0.0	2.5064855313122705E-5	0.0	0.45659393681149973	5.890240998583836E-4
48-49	0.0	2.5064855313122705E-5	0.0	0.55969821914203	6.266213828280676E-4
50-51	0.0	2.5064855313122705E-5	0.0	0.6748837617334854	6.266213828280676E-4
52-53	0.0	2.5064855313122705E-5	0.0	0.7959595453235246	7.268808040805585E-4
54-55	0.0	3.759728296968406E-5	0.0	0.9354705299963656	7.770105147068038E-4
56-57	0.0	5.012971062624541E-5	0.0	1.0979283897083705	8.020753700199266E-4
58-59	0.0	5.012971062624541E-5	0.0	1.2725677691025528	8.271402253330493E-4
60-61	0.0	5.012971062624541E-5	0.0	1.4609802864912962	8.271402253330493E-4
62-63	0.0	5.012971062624541E-5	0.0	1.657952451969471	8.772699359592946E-4
64-65	0.0	5.012971062624541E-5	0.0	1.8772824683869511	9.148672189289787E-4
66-67	0.0	5.012971062624541E-5	0.0	2.1328312007318937	9.399320742421015E-4
68-69	0.0	5.012971062624541E-5	0.0	2.413946085496222	9.524645018986628E-4
70-71	0.0	5.012971062624541E-5	0.0	2.705901520183475	9.90061784868347E-4
72-73	0.0	5.012971062624541E-5	0.0	3.016630531500257	0.001140450916747083
74-75	0.0	5.012971062624541E-5	0.0	3.3460077951700025	0.0011780481997167671
76-77	0.0	5.012971062624541E-5	0.0	3.7193112177759957	0.0011780481997167671
78-79	0.0	6.266213828280676E-5	0.0	4.129447445264622	0.0011905806273733285
80-81	0.0	7.519456593936812E-5	0.0	4.566816638050957	0.001240710337999574
82-83	0.0	7.519456593936812E-5	0.0	5.022082137530861	0.0014036318975348715
84-85	0.0	7.519456593936812E-5	0.0	5.494003233366335	0.0014036318975348715
86-87	0.0	7.519456593936812E-5	0.0	6.009775293572117	0.001416164325191433
88-89	0.0	7.519456593936812E-5	0.0	6.576165829082751	0.001453761608161117
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3355	0.0	52.281796	1
GTATCAA	6625	0.0	46.4232	1
TCAACGC	8535	0.0	34.643803	4
CAACGCA	8650	0.0	34.08442	5
TATCAAC	8905	0.0	33.310955	2
ATCAACG	9000	0.0	32.85387	3
AACGCAG	9205	0.0	32.181103	6
GTACATG	12030	0.0	28.805708	1
TACATGG	12450	0.0	27.218855	2
ACATGGG	13040	0.0	25.586962	3
ACGCAGA	11450	0.0	25.248133	7
CGCAGAG	11825	0.0	24.367035	8
CATGGGG	10570	0.0	23.887882	4
ATGGGGG	6445	0.0	21.10199	5
GCAGAGT	13115	0.0	21.100166	9
AGAGTAC	11260	0.0	19.382307	10-11
CGTATAC	245	1.7738239E-7	19.37202	3
GAGTACA	9090	0.0	19.035057	1
ACCGTAT	365	1.1641532E-10	18.237545	8
TATACCG	425	3.6379788E-12	17.902403	5
>>END_MODULE