FastQCFastQC Report
Mon 15 Aug 2016
SRR2921384_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921384_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497371
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36800.053.0047841
GTATCAA64000.041.156091
TCAACGC75200.034.083214
CAACGCA77950.032.8742035
ATCAACG79150.032.321853
TATCAAC80400.032.1742
AACGCAG83150.030.9789056
GTACATG127750.029.4600431
TACATGG129350.028.401362
ACATGGG136450.026.2622413
ACGCAGA97850.025.6010327
CGCAGAG99400.024.9175768
CATGGGG104200.024.0053184
ATGGGGG70150.020.835345
GCAGAGT111550.020.6300379
GAGTACA92300.019.2819371
CAGAGTA101400.018.5071510-11
AGAGTAC92550.018.09794410-11
GTGTAAG17900.017.4989511
GTATAGT8850.017.1604141
Produced by FastQC (version 0.11.5)