##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921384_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3497371 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.96472578974321 31.0 31.0 34.0 26.0 34.0 2 30.02176491999276 31.0 31.0 34.0 25.0 34.0 3 30.064788951472405 31.0 31.0 34.0 25.0 34.0 4 33.522131624011294 37.0 35.0 37.0 28.0 37.0 5 33.50928311580327 37.0 35.0 37.0 28.0 37.0 6 33.55263482198486 37.0 35.0 37.0 28.0 37.0 7 33.46294202130686 37.0 35.0 37.0 28.0 37.0 8 33.41742554621743 37.0 35.0 37.0 27.0 37.0 9 34.92062723685877 39.0 35.0 39.0 27.0 39.0 10-11 34.80034788988643 39.0 35.0 39.0 27.0 39.0 12-13 34.67785831128582 39.0 35.0 39.0 27.0 39.0 14-15 35.743088880190285 40.0 36.0 41.0 27.0 41.0 16-17 35.67977746713174 40.0 36.0 41.0 27.0 41.0 18-19 35.635636882675584 40.0 36.0 41.0 26.5 41.0 20-21 35.57310119515488 40.0 36.0 41.0 26.0 41.0 22-23 35.45790809725362 40.0 36.0 41.0 25.0 41.0 24-25 35.34753318993038 40.0 35.5 41.0 25.0 41.0 26-27 35.1894237128403 39.5 35.0 41.0 24.0 41.0 28-29 35.04459378201512 39.0 35.0 41.0 24.0 41.0 30-31 34.89703322867376 39.0 35.0 41.0 23.5 41.0 32-33 34.711051100955544 39.0 34.5 41.0 22.5 41.0 34-35 34.54404808640547 39.0 34.0 41.0 21.5 41.0 36-37 34.36602036215203 39.0 34.0 40.5 19.5 41.0 38-39 34.591916899865645 39.0 34.5 41.0 21.5 41.0 40-41 34.71668576196234 39.0 35.0 41.0 21.0 41.0 42-43 34.5571517862989 39.0 35.0 41.0 18.5 41.0 44-45 34.43846663679661 39.0 35.0 41.0 17.0 41.0 46-47 34.28098191470107 39.0 34.0 41.0 15.0 41.0 48-49 34.13367998419384 39.0 34.0 41.0 13.5 41.0 50-51 32.45249445940966 37.0 32.0 39.5 10.5 40.0 52-53 32.6059814643628 37.5 32.5 39.5 8.0 40.0 54-55 33.4587713170836 38.0 33.0 40.0 8.0 41.0 56-57 33.483563510991544 38.0 33.0 41.0 7.5 41.0 58-59 33.30887372257619 38.0 33.0 40.0 4.5 41.0 60-61 33.067454239198526 37.5 33.0 40.0 2.0 41.0 62-63 32.7817088893343 37.0 33.0 40.0 2.0 41.0 64-65 32.502524753593484 36.5 32.5 40.0 2.0 41.0 66-67 32.165365212898486 36.0 32.0 39.0 2.0 41.0 68-69 31.80207561622716 35.5 32.0 39.0 2.0 41.0 70-71 31.419115958815922 35.0 31.5 38.5 2.0 40.5 72-73 31.06317531082633 35.0 31.0 37.5 2.0 40.0 74-75 30.668332727640276 35.0 31.0 37.0 2.0 39.0 76-77 30.29488735967674 35.0 31.0 36.5 2.0 39.0 78-79 29.947022349073062 35.0 30.5 36.0 2.0 38.5 80-81 29.59832171079362 35.0 30.0 35.5 2.0 37.0 82-83 29.294306923686392 35.0 30.0 35.0 2.0 37.0 84-85 29.055014752509813 35.0 30.0 35.0 2.0 36.5 86-87 28.82690741130981 34.0 30.0 35.0 2.0 36.0 88-89 28.619041131181106 34.0 29.5 35.0 2.0 36.0 90-91 28.362164465823042 34.0 29.0 35.0 2.0 35.5 92-93 28.164376899105072 34.0 29.0 35.0 2.0 35.0 94-95 27.941571540451385 34.0 29.0 35.0 2.0 35.0 96-97 27.677213684221663 34.0 29.0 35.0 2.0 35.0 98-99 27.434335676712593 34.0 29.0 35.0 2.0 35.0 100-101 26.383260025888017 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 146736.0 3 24085.0 4 16447.0 5 12338.0 6 11130.0 7 11394.0 8 12212.0 9 12998.0 10 13626.0 11 14846.0 12 14734.0 13 14782.0 14 15686.0 15 16435.0 16 17097.0 17 17588.0 18 16477.0 19 15325.0 20 15899.0 21 16692.0 22 18503.0 23 21367.0 24 24713.0 25 29501.0 26 34534.0 27 40834.0 28 47920.0 29 56098.0 30 66497.0 31 80807.0 32 100325.0 33 129009.0 34 180423.0 35 254581.0 36 409294.0 37 710548.0 38 727341.0 39 128533.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.89815477041911 18.32653411529109 13.309884136747247 24.465426977542553 2 15.855683936319199 19.52060585166419 39.701680425835676 24.92202978618094 3 19.192476610275 23.975211926471125 29.437782644915593 27.394528818338276 4 13.442962422978736 15.786651850584544 36.65340982078511 34.116975905651614 5 14.14982096058501 37.718256584922926 34.11727146048503 14.014650994007036 6 33.32767085318419 35.094021479102736 17.236961353001014 14.341346314712066 7 28.994629406146156 32.23831938732906 20.678359194925676 18.08869201159911 8 26.997819294595427 34.60912464340104 19.904535152256138 18.488520909747397 9 27.48521989411404 15.48301889382576 19.44930949907796 37.58245171298224 10-11 25.926062038288133 25.350654706552845 27.436143428131572 21.287139827027456 12-13 26.2504792498576 22.842438949538654 27.363540172399816 23.543541628203933 14-15 23.590812583780593 24.346876110280576 24.76291129369535 27.29940001224348 16-17 23.58519491395505 27.527248712357476 25.687595958255443 23.199960415432034 18-19 23.680784940342836 26.925953959085895 26.90371756722147 22.4895435333498 20-21 24.212239506989906 26.034185457666588 26.93288151609699 22.820693519246515 22-23 24.205792165297872 25.751612073624013 26.512485650034485 23.53011011104363 24-25 23.88850048829773 26.00804732969508 26.508702684178637 23.59474949782855 26-27 23.560533406859875 26.27464681373692 26.546281251045766 23.618538528357437 28-29 23.585996329905807 26.60959855609978 26.365135334902174 23.439269779092246 30-31 24.18249422887059 26.108482219774043 26.226070992219608 23.482952559135757 32-33 23.87974576220296 26.189355515134082 26.045519626449902 23.885379096213054 34-35 24.199202673444546 26.31266278845825 26.1938630475901 23.2942714905071 36-37 24.24579607872291 26.094106075205943 26.262849087732643 23.397248758338502 38-39 23.98625454119134 26.33442040744548 26.166684061758772 23.512640989604407 40-41 24.165231732974803 26.07308354144876 26.216681754677523 23.545002970898913 42-43 23.901277584037988 26.055297540995703 26.059882909743177 23.983541965223132 44-45 23.78476619308968 26.095359274059998 26.281202045889255 23.838672486961066 46-47 24.024154208720443 26.044898748594225 25.994799538691858 23.936147503993467 48-49 23.751654468357483 26.260658687207027 26.04549721725549 23.942189627179996 50-51 23.919303527046836 26.178018571637935 26.22029391030265 23.682383991012586 52-53 24.148220438698047 26.039320848536757 26.17807317784954 23.63438553491566 54-55 23.510832341958864 25.891248512938297 26.529653557601723 24.068265587501116 56-57 23.648152109546523 25.998801328081488 26.301420854627573 24.051625707744414 58-59 23.747154235085613 25.94896320313138 26.405732649251323 23.898149912531682 60-61 23.905161729412185 26.027338181036647 26.528925027760863 23.538575061790308 62-63 23.886066797737016 25.901094325662537 26.566332228089916 23.646506648510528 64-65 23.554033098445544 25.697209243081137 26.595271400920513 24.153486257552807 66-67 23.57430153271333 25.895885520346756 26.389588695299 24.140224251640912 68-69 23.612279882904108 25.817626902829723 26.493403829254547 24.076689385011623 70-71 23.59937525925824 25.629009192351855 26.465897658024346 24.305717890365564 72-73 23.52468626918716 25.947337773540823 26.309611719397413 24.218364237874603 74-75 23.370372773740833 26.06459562663293 26.457150379669663 24.107881219956575 76-77 23.40321999580293 25.598730279692283 26.543442870106492 24.45460685439829 78-79 23.338298625956998 25.582008140863767 26.65803328560997 24.42165994756926 80-81 23.58375346904829 25.807225880522445 26.284663793747047 24.324356856682215 82-83 23.5935907865156 25.778629392169623 26.500560782536315 24.127219038778467 84-85 23.49465194360669 25.842787431522986 26.414460901472193 24.248099723398134 86-87 23.209380041500726 26.036368097322487 26.45266519426362 24.301586666913167 88-89 23.167914595761687 25.66382055617028 26.597664287177082 24.57060056089095 90-91 23.329988980977603 25.50147729993968 26.730293913684754 24.43823980539796 92-93 23.483264085540025 25.308720091252017 26.77531339935127 24.432702423856686 94-95 23.295238570281096 25.3296491291438 26.764674180700297 24.610438119874807 96-97 23.1660637861318 25.10178401707492 26.93539414942388 24.796758047369398 98-99 22.9060949625721 25.051404153521528 26.890230365992412 25.152270517913955 100-101 23.087870304384744 25.037466883231176 27.076101059105945 24.798561753278136 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 25.0 1 24.5 2 32.5 3 48.5 4 56.0 5 87.0 6 143.0 7 241.5 8 356.5 9 422.0 10 433.5 11 443.5 12 506.5 13 574.5 14 652.0 15 772.5 16 893.5 17 991.0 18 1112.0 19 1247.5 20 1363.5 21 1478.5 22 1629.0 23 1828.5 24 2179.5 25 2750.5 26 3718.0 27 5462.5 28 8026.5 29 11783.0 30 17041.5 31 23922.5 32 33549.5 33 41671.0 34 49005.5 35 57912.0 36 69039.0 37 80186.0 38 88728.5 39 97030.5 40 102683.5 41 107139.0 42 112316.5 43 118435.5 44 124880.5 45 133310.5 46 138687.0 47 141546.5 48 146945.0 49 149751.5 50 149525.5 51 147644.5 52 145147.5 53 143559.5 54 140885.0 55 134205.0 56 124419.5 57 112420.5 58 101230.5 59 87854.5 60 71994.0 61 59456.5 62 49634.0 63 38842.0 64 27873.0 65 20153.0 66 14835.0 67 11256.5 68 8512.0 69 6262.5 70 4759.0 71 3597.5 72 2607.5 73 1962.5 74 1462.5 75 1078.5 76 796.5 77 537.5 78 385.0 79 280.0 80 191.0 81 148.0 82 123.5 83 104.0 84 92.0 85 73.5 86 56.0 87 59.0 88 62.5 89 48.5 90 38.0 91 29.5 92 26.0 93 21.5 94 15.5 95 14.5 96 11.5 97 9.0 98 9.5 99 5.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05349732699218928 2 0.06078851800395211 3 0.05201049588390823 4 0.05918731527195714 5 0.050666629305269585 6 0.019157246972082743 7 0.01575469116659342 8 0.02275995311907144 9 0.022273873718287252 10-11 0.02300299281946353 12-13 0.029207653405944067 14-15 0.04669221538121063 16-17 0.03027988737826213 18-19 0.024318266492173692 20-21 0.02779230456248422 22-23 0.04912261238513157 24-25 0.044404782906932096 26-27 0.032252797887327364 28-29 0.020887117780755887 30-31 0.012537989249639229 32-33 0.009435658956398963 34-35 0.008849504384865089 36-37 0.00117230914306775 38-39 0.006176067680552049 40-41 0.003230998369918433 42-43 0.22907206584603118 44-45 0.20172295132543844 46-47 0.20861384165420252 48-49 0.205911812043961 50-51 0.20462513127717935 52-53 0.2098576330620915 54-55 0.20778464738227656 56-57 0.21946484945406133 58-59 0.21547613907703816 60-61 0.2214806493220193 62-63 0.2185069871054572 64-65 0.2198937430429886 66-67 0.2092571820375934 68-69 0.2070126389222076 70-71 0.189942674082904 72-73 0.1978200196662007 74-75 0.19736253317134497 76-77 0.19553258719192218 78-79 0.22345355983108456 80-81 0.23221728549816417 82-83 0.23018718917724199 84-85 0.22528350581050735 86-87 0.2435114833399145 88-89 0.24182450189013405 90-91 0.2403090778759245 92-93 0.24508409316598098 94-95 0.2563354016488385 96-97 0.25674999878480154 98-99 0.245798915814193 100-101 0.25610665840141067 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3497371.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.00841582603476 #Duplication Level Percentage of deduplicated Percentage of total 1 75.33877668252983 40.689279788948426 2 12.941453761407601 13.97894832279007 3 4.632334839220682 7.505551987261754 4 2.255296967045588 4.872200656295726 5 1.230068786124704 3.3217033247824412 6 0.7682782167137306 2.489609363901577 7 0.552143541906633 2.087427860486716 8 0.38158125709060997 1.6486879363496594 9 0.27166366595519403 1.320491181215981 >10 1.5058302280625937 14.52745963859597 >50 0.08491642396978664 3.117730259110802 >100 0.03530604895058001 3.240606855389135 >500 0.0013883887901873543 0.5007822946739527 >1k 9.611922323494607E-4 0.6995205301977009 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.8592905928481707E-5 0.0 5 0.0 0.0 0.0 2.8592905928481707E-5 0.0 6 0.0 0.0 0.0 5.718581185696341E-5 2.8592905928481707E-5 7 0.0 0.0 0.0 5.718581185696341E-5 2.8592905928481707E-5 8 0.0 0.0 0.0 5.718581185696341E-5 2.8592905928481707E-5 9 0.0 0.0 0.0 5.718581185696341E-5 5.718581185696341E-5 10-11 0.0 0.0 0.0 8.577871778544513E-5 5.718581185696341E-5 12-13 0.0 0.0 0.0 8.577871778544513E-5 8.577871778544513E-5 14-15 0.0 0.0 0.0 1.1437162371392684E-4 1.858538885351311E-4 16-17 0.0 0.0 0.0 1.858538885351311E-4 2.0015034149937195E-4 18-19 0.0 0.0 0.0 2.573361533563354E-4 2.573361533563354E-4 20-21 0.0 0.0 0.0 2.573361533563354E-4 2.573361533563354E-4 22-23 0.0 0.0 0.0 2.573361533563354E-4 3.145219652132988E-4 24-25 0.0 0.0 0.0 4.003006829987439E-4 3.717077770702622E-4 26-27 0.0 0.0 0.0 5.861545715338751E-4 3.717077770702622E-4 28-29 0.0 0.0 0.0 0.002072985679814924 3.8600423003450307E-4 30-31 0.0 0.0 0.0 0.008434907248902104 4.1459713596298474E-4 32-33 0.0 0.0 0.0 0.02428967358624521 4.574864948557073E-4 34-35 0.0 0.0 0.0 0.0482219358483844 5.432652126411524E-4 36-37 0.0 0.0 1.4296452964240853E-5 0.08677946949294199 5.718581185696342E-4 38-39 0.0 0.0 2.8592905928481707E-5 0.14975534480042296 5.861545715338751E-4 40-41 0.0 0.0 2.8592905928481707E-5 0.22079441957973575 6.290439304265976E-4 42-43 0.0 0.0 2.8592905928481707E-5 0.29672288127281893 6.719332893193202E-4 44-45 0.0 0.0 2.8592905928481707E-5 0.3923947445095187 7.005261952478018E-4 46-47 0.0 0.0 2.8592905928481707E-5 0.5050650903207009 7.577120071047652E-4 48-49 0.0 0.0 2.8592905928481707E-5 0.6261560469278209 8.577871778544513E-4 50-51 0.0 0.0 2.8592905928481707E-5 0.7560822114668418 8.577871778544513E-4 52-53 0.0 0.0 2.8592905928481707E-5 0.8919414039860227 9.578623486041372E-4 54-55 0.0 0.0 2.8592905928481707E-5 1.051275372272487 0.0010007517074968597 56-57 0.0 0.0 2.8592905928481707E-5 1.2380299373443653 0.0010007517074968597 58-59 0.0 0.0 2.8592905928481707E-5 1.4360644038050294 0.0010007517074968597 60-61 0.0 0.0 2.8592905928481707E-5 1.645150028407052 0.0010293446134253415 62-63 0.0 0.0 2.8592905928481707E-5 1.8655012579448962 0.0010293446134253415 64-65 0.0 0.0 2.8592905928481707E-5 2.1084837725251337 0.0010436410663895823 66-67 0.0 0.0 2.8592905928481707E-5 2.397729608897655 0.001072233972318064 68-69 0.0 0.0 2.8592905928481707E-5 2.7134381797069853 0.0010865304252823049 70-71 0.0 0.0 2.8592905928481707E-5 3.0459450827492995 0.0011437162371392684 72-73 0.0 0.0 2.8592905928481707E-5 3.3916476118776075 0.0013867559375313628 74-75 0.0 0.0 2.8592905928481707E-5 3.7578226616507084 0.0014010523904956038 76-77 1.4296452964240853E-5 0.0 2.8592905928481707E-5 4.172005200477731 0.0014010523904956038 78-79 2.8592905928481707E-5 0.0 2.8592905928481707E-5 4.6351387942543125 0.0014010523904956038 80-81 2.8592905928481707E-5 0.0 2.8592905928481707E-5 5.118859280299402 0.0014296452964240856 82-83 2.8592905928481707E-5 0.0 2.8592905928481707E-5 5.622980804724463 0.0014868311082810487 84-85 2.8592905928481707E-5 0.0 2.8592905928481707E-5 6.149133163167419 0.0014868311082810487 86-87 2.8592905928481707E-5 0.0 2.8592905928481707E-5 6.7152155147395 0.0015297204671737713 88-89 2.8592905928481707E-5 0.0 2.8592905928481707E-5 7.327260962591615 0.0015726098260664938 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3680 0.0 53.004784 1 GTATCAA 6400 0.0 41.15609 1 TCAACGC 7520 0.0 34.08321 4 CAACGCA 7795 0.0 32.874203 5 ATCAACG 7915 0.0 32.32185 3 TATCAAC 8040 0.0 32.174 2 AACGCAG 8315 0.0 30.978905 6 GTACATG 12775 0.0 29.460043 1 TACATGG 12935 0.0 28.40136 2 ACATGGG 13645 0.0 26.262241 3 ACGCAGA 9785 0.0 25.601032 7 CGCAGAG 9940 0.0 24.917576 8 CATGGGG 10420 0.0 24.005318 4 ATGGGGG 7015 0.0 20.83534 5 GCAGAGT 11155 0.0 20.630037 9 GAGTACA 9230 0.0 19.281937 1 CAGAGTA 10140 0.0 18.50715 10-11 AGAGTAC 9255 0.0 18.097944 10-11 GTGTAAG 1790 0.0 17.498951 1 GTATAGT 885 0.0 17.160414 1 >>END_MODULE