FastQCFastQC Report
Mon 15 Aug 2016
SRR2921384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497371
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35400.059.082681
GTATCAA62300.041.5070271
TCAACGC80250.032.0566864
CAACGCA83250.030.961715
TATCAAC85200.030.58422
ATCAACG84550.030.4263633
AACGCAG88150.029.075046
GTACATG137500.027.1379971
TACATGG139500.026.2327942
ACATGGG145850.024.6025283
CATGGGG108800.023.20854
ACGCAGA108850.022.8468937
CGCAGAG108400.022.3714838
ATGGGGG68950.021.2435135
GTAAGTA24550.019.7202782
GCAGAGT125100.018.5488249
AGAGTAC106050.017.80056210-11
CAGAGTA115700.017.363910-11
TAAGTAC31250.017.1629833
GAGTACA104500.017.0579091
Produced by FastQC (version 0.11.5)