##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921384_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3497371 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.496095495730938 33.0 31.0 34.0 30.0 34.0 2 31.62037084427131 34.0 31.0 34.0 30.0 34.0 3 31.693587554766136 34.0 31.0 34.0 29.0 34.0 4 35.19680582929292 37.0 35.0 37.0 33.0 37.0 5 34.956039836780256 37.0 35.0 37.0 32.0 37.0 6 34.918906515779994 37.0 35.0 37.0 32.0 37.0 7 34.786961120224305 37.0 35.0 37.0 32.0 37.0 8 34.74828206672955 37.0 35.0 37.0 32.0 37.0 9 36.3704940082136 39.0 37.0 39.0 32.0 39.0 10-11 36.22571740315797 39.0 37.0 39.0 32.0 39.0 12-13 36.160920445671906 39.0 37.0 39.0 32.0 39.0 14-15 37.399760134112164 40.0 38.0 41.0 32.0 41.0 16-17 37.37708352931388 40.0 38.0 41.0 32.0 41.0 18-19 37.349526115473594 40.0 38.0 41.0 31.5 41.0 20-21 37.31179005601636 40.0 38.0 41.0 31.0 41.0 22-23 37.203335448255274 40.0 38.0 41.0 31.0 41.0 24-25 37.13622089277918 40.0 37.5 41.0 31.0 41.0 26-27 36.99794602860263 40.0 37.0 41.0 30.5 41.0 28-29 36.84844587548761 40.0 37.0 41.0 30.0 41.0 30-31 36.7122315590768 40.0 37.0 41.0 30.0 41.0 32-33 36.667376151972434 40.0 37.0 41.0 30.0 41.0 34-35 36.799534564677295 40.0 37.0 41.0 30.0 41.0 36-37 36.77843471567643 40.0 37.0 41.0 30.0 41.0 38-39 36.69089396006314 40.0 37.0 41.0 30.0 41.0 40-41 36.576476158806145 40.0 37.0 41.0 30.0 41.0 42-43 36.476636593601306 40.0 37.0 41.0 30.0 41.0 44-45 36.348010548494855 40.0 36.0 41.0 29.5 41.0 46-47 36.19452683172589 40.0 36.0 41.0 29.0 41.0 48-49 36.043368575996084 40.0 36.0 41.0 28.0 41.0 50-51 35.89437880625189 40.0 35.0 41.0 28.0 41.0 52-53 35.728524082803915 39.0 35.0 41.0 28.0 41.0 54-55 35.52105281367061 39.0 35.0 41.0 27.5 41.0 56-57 35.264308390502464 39.0 35.0 41.0 27.0 41.0 58-59 35.0374547052629 38.5 35.0 40.5 26.0 41.0 60-61 34.76740042734957 38.0 35.0 40.0 26.0 41.0 62-63 34.47100707931758 37.5 34.0 40.0 26.0 41.0 64-65 34.15626509169316 37.0 34.0 40.0 26.0 41.0 66-67 33.816813972552524 36.5 34.0 39.5 25.0 41.0 68-69 33.44889275401437 36.0 34.0 39.0 24.5 41.0 70-71 33.06542185544513 35.5 33.5 39.0 24.0 40.0 72-73 32.67119487752372 35.0 33.0 37.5 22.5 40.0 74-75 32.261890574377155 35.0 33.0 37.0 22.0 39.0 76-77 29.495999709496076 33.0 29.0 34.5 16.5 36.5 78-79 31.15734404499837 34.5 31.5 36.0 19.5 37.5 80-81 31.356250452125323 35.0 32.0 36.0 20.0 37.0 82-83 31.189881628228747 35.0 32.0 35.0 20.0 37.0 84-85 30.9582962459516 35.0 32.0 35.0 18.5 36.5 86-87 30.716288892428054 35.0 32.0 35.0 17.5 36.0 88-89 30.439876124094354 35.0 32.0 35.0 11.5 36.0 90-91 30.253354019347675 35.0 32.0 35.0 8.5 35.0 92-93 30.10830992765709 34.0 31.5 35.0 4.5 35.0 94-95 30.00574431480103 34.0 31.0 35.0 2.0 35.0 96-97 29.819133143152385 34.0 31.0 35.0 2.0 35.0 98-99 29.5537240687362 34.0 31.0 35.0 2.0 35.0 100-101 28.458565734089976 33.5 29.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55702.0 3 19652.0 4 10864.0 5 7834.0 6 6876.0 7 7025.0 8 7443.0 9 7939.0 10 8821.0 11 9734.0 12 10693.0 13 11412.0 14 12307.0 15 13698.0 16 14526.0 17 15880.0 18 14514.0 19 13240.0 20 12584.0 21 12517.0 22 13403.0 23 15364.0 24 18012.0 25 21670.0 26 25771.0 27 31087.0 28 37795.0 29 45168.0 30 55396.0 31 69084.0 32 87560.0 33 115959.0 34 168679.0 35 247161.0 36 425185.0 37 828077.0 38 897660.0 39 121076.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.60573725515802 19.911513801876602 14.05927641670132 25.423472526264064 2 14.82882754085236 20.777703118075113 38.60928989091741 25.784179450155115 3 17.75828756020278 25.07512569560432 29.494538249694962 27.67204849449793 4 13.002653252571974 16.924085637236693 35.852822401355134 34.2204387088362 5 13.857660656373957 38.04903398961958 34.220543411763735 13.872761942242729 6 33.08737392149715 35.772368881931186 17.099526066350712 14.040731130220955 7 28.654699378132175 32.436715963752164 20.8207488662185 18.08783579189716 8 26.78112655638143 35.39529836630882 19.652560049981485 18.171015027328263 9 26.960866868780503 15.650993646938598 19.506663437572644 37.88147604670826 10-11 25.79137183330688 25.733179202531964 27.455142941910843 21.02030602225031 12-13 26.09347758489433 23.029440042317603 27.51750783626233 23.35957453652574 14-15 23.373706713828913 24.605396255090756 24.802128020050628 27.218769011029703 16-17 23.435346816152563 27.796616566560335 25.6890581464986 23.078978470788503 18-19 23.515536585045055 27.143303602319236 26.962610061652253 22.378549750983456 20-21 24.068764754053362 26.129943179738678 26.97724330234601 22.82404876386195 22-23 24.020894036833194 25.90219798553853 26.608759203187887 23.46814877444039 24-25 23.79219215870449 26.217536726362418 26.48957379477271 23.50069732016038 26-27 23.43982770580744 26.45607693306272 26.627581939533478 23.476513421596362 28-29 23.45593210960931 26.80525791379964 26.375681569636445 23.3631284069546 30-31 24.04502858361915 26.2567505484586 26.270705983435644 23.4275148844866 32-33 23.774432458610363 26.37023306198033 26.098045975252937 23.757288504156374 34-35 24.05149569756301 26.48531340566474 26.271710752925404 23.191480143846853 36-37 24.052045194283092 26.26605324896457 26.369517448235737 23.312384108516603 38-39 23.9710782053619 26.41546308110755 26.196969859429785 23.41648885410077 40-41 24.11306045950649 26.181567384386717 26.217038532303693 23.488333623803097 42-43 23.85488226695308 26.176987324223976 26.003527530522746 23.964602878300198 44-45 23.683827501200284 26.19878922224214 26.277597580388957 23.839785696168615 46-47 23.919368061832667 26.141583294038316 26.019094374088336 23.919954270040673 48-49 23.612949403907 26.342161857111257 26.137142067890302 23.907746671091438 50-51 23.79910453616655 26.288022756608036 26.196549107252636 23.716323599972778 52-53 24.04599191292852 26.119213607141834 26.143846821722004 23.69094765820764 54-55 23.388032047213024 26.042563777041984 26.492119725276925 24.07728445046807 56-57 23.58029167650798 26.082306167114933 26.22865340524688 24.1087487511302 58-59 23.672504024469806 26.11076295997949 26.308272599905436 23.90846041564527 60-61 23.797622768396494 26.256622285995824 26.337417964006104 23.60833698160158 62-63 23.7264324149954 26.12903345733412 26.447815000892255 23.69671912677822 64-65 23.41234377428988 25.905019001433992 26.416834242253017 24.265802982023114 66-67 23.464338395451616 26.175588849171604 26.184927829560888 24.17514492581589 68-69 23.505643942887747 26.03767109330256 26.257737317663143 24.198947646146554 70-71 23.456474765198116 25.87855120503123 26.25559007827969 24.409383951490966 72-73 23.473150002795677 26.16911077353269 26.054824127695593 24.302915095976044 74-75 23.28863402171684 26.290651153465888 26.119325083103366 24.3013897417139 76-77 23.349016109767586 25.79077681910987 26.308655270235526 24.551551800887022 78-79 23.273580304634148 25.81998095231828 26.358334762791696 24.548103980255878 80-81 23.559686160367985 26.061975188239554 25.981013477550075 24.39732517384239 82-83 23.531297585269012 26.059879311353175 26.156728225474225 24.25209487790359 84-85 23.42697625462637 26.05116610320743 26.0652096812022 24.456647960964002 86-87 23.15357666385033 26.326383587888657 26.03883110351503 24.481208644745983 88-89 23.062036338637316 26.01378731831613 26.085917595458657 24.838258747587894 90-91 23.167537877820845 25.822793270385063 26.284091042736918 24.72557780905717 92-93 23.33818103691078 25.70268457499057 26.23620374888123 24.722930639217424 94-95 23.12857574225604 25.739582451669573 26.254712373640903 24.877129432433485 96-97 22.950469955706048 25.5645695276651 26.381474750273696 25.103485766355156 98-99 22.67594502523937 25.60090400804732 26.275321575357545 25.447829391355764 100-101 22.7218016713033 25.670665097279194 26.353843309918805 25.253689921498694 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 17.0 1 15.5 2 20.0 3 33.5 4 37.0 5 43.5 6 66.0 7 135.5 8 218.5 9 263.0 10 272.5 11 256.5 12 264.5 13 297.5 14 349.0 15 380.5 16 412.5 17 491.5 18 572.0 19 631.5 20 724.5 21 869.0 22 1033.5 23 1294.5 24 1685.0 25 2315.5 26 3439.5 27 5323.5 28 8068.5 29 12086.5 30 17719.5 31 25060.5 32 34988.5 33 42851.5 34 49844.5 35 58926.0 36 70537.5 37 82017.0 38 90038.5 39 98076.0 40 103893.5 41 107956.0 42 112706.5 43 118695.0 44 125908.0 45 133982.0 46 139464.0 47 143193.5 48 148255.5 49 151021.5 50 150831.5 51 148570.0 52 145564.0 53 144438.0 54 142081.0 55 135142.0 56 124859.0 57 112031.5 58 100109.5 59 86827.0 60 71063.0 61 58256.0 62 48106.0 63 36917.5 64 25886.0 65 18384.5 66 13459.0 67 10177.0 68 7643.5 69 5648.5 70 4253.5 71 3104.5 72 2205.5 73 1577.5 74 1208.5 75 875.0 76 570.5 77 315.5 78 180.0 79 102.5 80 73.5 81 55.0 82 29.5 83 22.0 84 17.5 85 11.5 86 9.0 87 8.0 88 4.5 89 2.5 90 1.0 91 1.5 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1580329910667184 2 6.004510244981159E-4 3 0.006633554175407756 4 0.004403307512986183 5 0.028021047809912075 6 0.0013152736727101587 7 1.1437162371392684E-4 8 0.00217306085056461 9 5.718581185696342E-5 10-11 0.10869593188712322 12-13 2.4303970039209454E-4 14-15 0.006390514475015662 16-17 1.858538885351311E-4 18-19 3.717077770702622E-4 20-21 7.720084600690061E-4 22-23 2.7163260632057627E-4 24-25 0.0015297204671737715 26-27 0.0027735118750627256 28-29 0.005847249262374509 30-31 0.015025572065417136 32-33 0.014625271382418394 34-35 0.013081254462280381 36-37 7.434155541405244E-4 38-39 2.4303970039209454E-4 40-41 0.004646347213378277 42-43 0.008778022120043884 44-45 0.008006013659974878 46-47 0.009149729897114147 48-49 0.01119412267100059 50-51 0.0054040592204830425 52-53 0.0017298708086731433 54-55 8.863800837829329E-4 56-57 0.005975917339052677 58-59 0.009335583785649278 60-61 0.007948827848117915 62-63 0.017699008769730178 64-65 0.03395407579007203 66-67 0.03637017634102873 68-69 0.026405548624952858 70-71 0.04131674906665607 72-73 0.025719318882669297 74-75 0.023503368673211966 76-77 0.24641366329165537 78-79 0.0702670663192438 80-81 0.05408348156372315 82-83 0.019657622825831174 84-85 0.03176671848654318 86-87 0.0823046797151346 88-89 0.264141264967314 90-91 0.32104114776499265 92-93 0.05436941062300797 94-95 0.005575616656053934 96-97 0.01122271557692907 98-99 0.0016297956379234572 100-101 0.0011580126901035092 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3497371.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.92716903934338 #Duplication Level Percentage of deduplicated Percentage of total 1 73.53869070917042 38.186560233870836 2 13.732187929915202 14.261472878334752 3 5.045757628788739 7.860357279650078 4 2.434468405135802 5.056602095777099 5 1.3749435306553863 3.5698462567946927 6 0.8451275837972942 2.6331049742192363 7 0.5573898727810687 2.0260574701304455 8 0.3993662445327523 1.659036679076798 9 0.30382516534707554 1.4199102647445643 >10 1.628624786436967 15.158032063006416 >50 0.09929845142821372 3.4982688667230124 >100 0.03749877677878344 3.3410664752401535 >500 0.0018252980951894702 0.6414194510813288 >1k 9.956171369619497E-4 0.6882650113506081 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.8592905928481707E-5 5 0.0 0.0 0.0 0.0 2.8592905928481707E-5 6 0.0 0.0 0.0 0.0 2.8592905928481707E-5 7 0.0 0.0 0.0 0.0 2.8592905928481707E-5 8 0.0 0.0 0.0 0.0 5.718581185696341E-5 9 0.0 0.0 0.0 5.718581185696341E-5 5.718581185696341E-5 10-11 0.0 0.0 0.0 5.718581185696341E-5 5.718581185696341E-5 12-13 0.0 0.0 0.0 5.718581185696341E-5 1.1437162371392683E-4 14-15 0.0 0.0 0.0 5.718581185696341E-5 2.1444679446361282E-4 16-17 0.0 0.0 0.0 5.718581185696341E-5 2.573361533563354E-4 18-19 0.0 0.0 0.0 5.718581185696341E-5 2.573361533563354E-4 20-21 0.0 0.0 0.0 1.286680766781677E-4 2.7163260632057627E-4 22-23 0.0 0.0 0.0 1.4296452964240855E-4 3.717077770702622E-4 24-25 0.0 0.0 0.0 2.716326063205762E-4 4.574864948557073E-4 26-27 0.0 0.0 0.0 4.574864948557073E-4 5.003758537484299E-4 28-29 0.0 0.0 0.0 0.002058689226850683 5.146723067126708E-4 30-31 0.0 0.0 0.0 0.00853498241965179 5.146723067126708E-4 32-33 0.0 0.0 0.0 0.02476145653406516 5.861545715338751E-4 34-35 0.0 0.0 0.0 0.049622988238880006 6.147474774623567E-4 36-37 0.0 0.0 0.0 0.08953868491504047 6.576368363550793E-4 38-39 0.0 0.0 0.0 0.15417294876637339 7.005261952478018E-4 40-41 0.0 0.0 0.0 0.22625566461207575 7.148226482120427E-4 42-43 0.0 0.0 0.0 0.30369955031936846 7.863049130332469E-4 44-45 0.0 0.0 0.0 0.4004722404343148 8.291942719259695E-4 46-47 0.0 0.0 0.0 0.5150726073956695 8.434907248902104E-4 48-49 0.0 0.0 0.0 0.6378934348114627 8.72083630818692E-4 50-51 0.0 0.0 2.8592905928481707E-5 0.7698782885773343 8.863800837829329E-4 52-53 1.4296452964240853E-5 0.0 2.8592905928481707E-5 0.9076532057937234 9.578623486041372E-4 54-55 2.8592905928481707E-5 0.0 2.8592905928481707E-5 1.068302447752898 0.0010007517074968597 56-57 2.8592905928481707E-5 0.0 2.8592905928481707E-5 1.2569155517101274 0.0010007517074968597 58-59 2.8592905928481707E-5 0.0 2.8592905928481707E-5 1.4570658932094993 0.0010293446134253415 60-61 2.8592905928481707E-5 0.0 2.8592905928481707E-5 1.6682530963972653 0.001072233972318064 62-63 2.8592905928481707E-5 0.0 2.8592905928481707E-5 1.891592284604636 0.0011437162371392684 64-65 5.718581185696341E-5 0.0 2.8592905928481707E-5 2.138234691143719 0.0011437162371392684 66-67 5.718581185696341E-5 0.0 2.8592905928481707E-5 2.429853738708304 0.0011723091430677502 68-69 5.718581185696341E-5 0.0 2.8592905928481707E-5 2.749651095065408 0.0012009020489962318 70-71 5.718581185696341E-5 0.0 2.8592905928481707E-5 3.0873190176278125 0.001286680766781677 72-73 5.718581185696341E-5 0.0 2.8592905928481707E-5 3.4381396769173187 0.0015297204671737715 74-75 5.718581185696341E-5 0.0 2.8592905928481707E-5 3.810976873771756 0.001558313373102253 76-77 5.718581185696341E-5 0.0 2.8592905928481707E-5 4.233151129805789 0.0016012027319949756 78-79 5.718581185696341E-5 0.0 2.8592905928481707E-5 4.705949125786198 0.0016012027319949756 80-81 5.718581185696341E-5 0.0 2.8592905928481707E-5 5.20185018975682 0.001658388543851939 82-83 5.718581185696341E-5 0.0 2.8592905928481707E-5 5.717408876553273 0.0018013530734943477 84-85 5.718581185696341E-5 0.0 2.8592905928481707E-5 6.256285078134404 0.0018013530734943477 86-87 5.718581185696341E-5 0.0 2.8592905928481707E-5 6.834748157973518 0.0018299459794228292 88-89 5.718581185696341E-5 0.0 2.8592905928481707E-5 7.4587597369567025 0.0019157246972082743 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3540 0.0 59.08268 1 GTATCAA 6230 0.0 41.507027 1 TCAACGC 8025 0.0 32.056686 4 CAACGCA 8325 0.0 30.96171 5 TATCAAC 8520 0.0 30.5842 2 ATCAACG 8455 0.0 30.426363 3 AACGCAG 8815 0.0 29.07504 6 GTACATG 13750 0.0 27.137997 1 TACATGG 13950 0.0 26.232794 2 ACATGGG 14585 0.0 24.602528 3 CATGGGG 10880 0.0 23.2085 4 ACGCAGA 10885 0.0 22.846893 7 CGCAGAG 10840 0.0 22.371483 8 ATGGGGG 6895 0.0 21.243513 5 GTAAGTA 2455 0.0 19.720278 2 GCAGAGT 12510 0.0 18.548824 9 AGAGTAC 10605 0.0 17.800562 10-11 CAGAGTA 11570 0.0 17.3639 10-11 TAAGTAC 3125 0.0 17.162983 3 GAGTACA 10450 0.0 17.057909 1 >>END_MODULE