FastQCFastQC Report
Mon 15 Aug 2016
SRR2921383_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921383_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3860672
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30450.048.6184461
GTATCAA56750.041.6387061
TCAACGC66050.035.565434
CAACGCA66650.035.242525
ATCAACG69450.033.7555163
TATCAAC70800.033.0452732
AACGCAG72400.032.4973146
GTACATG113950.028.8570981
TACATGG117750.027.3655742
ACGCAGA85750.026.5030427
ACATGGG125200.025.2441043
CGCAGAG90450.024.2878638
CATGGGG103050.022.7956964
GTATAGG12050.019.6886861
GCAGAGT104550.019.6508229
ATGGGGG68600.019.2993185
TAGCCCT22200.018.3840484
GAGTACA89500.018.290681
GTATAGC10000.017.0819031
AGAGTAC89000.016.9537310-11
Produced by FastQC (version 0.11.5)