##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921383_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3860672
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.939963042703447	31.0	31.0	34.0	26.0	34.0
2	29.992804620542746	31.0	31.0	34.0	25.0	34.0
3	30.04217219178423	31.0	31.0	34.0	25.0	34.0
4	33.492849949438856	37.0	35.0	37.0	28.0	37.0
5	33.48027260539098	37.0	35.0	37.0	28.0	37.0
6	33.5222880887058	37.0	35.0	37.0	28.0	37.0
7	33.43142877716625	37.0	35.0	37.0	27.0	37.0
8	33.38852044410921	37.0	35.0	37.0	27.0	37.0
9	34.89082807345457	39.0	35.0	39.0	27.0	39.0
10-11	34.7703797421796	39.0	35.0	39.0	27.0	39.0
12-13	34.64807214909736	39.0	35.0	39.0	27.0	39.0
14-15	35.72087851545016	40.0	36.0	41.0	27.0	41.0
16-17	35.666934927390884	40.0	36.0	41.0	27.0	41.0
18-19	35.61758289748521	40.0	36.0	41.0	26.0	41.0
20-21	35.55209870198763	40.0	36.0	41.0	26.0	41.0
22-23	35.434064846741705	40.0	36.0	41.0	25.0	41.0
24-25	35.330107944938085	40.0	36.0	41.0	25.0	41.0
26-27	35.17576693901994	39.5	35.0	41.0	24.0	41.0
28-29	35.0321611108118	39.0	35.0	41.0	24.0	41.0
30-31	34.887743118296505	39.0	35.0	41.0	23.0	41.0
32-33	34.70659434419707	39.0	34.5	41.0	22.5	41.0
34-35	34.54454379962867	39.0	34.0	41.0	21.5	41.0
36-37	34.37072043416276	39.0	34.0	40.5	19.0	41.0
38-39	34.6058243228122	39.0	35.0	41.0	21.5	41.0
40-41	34.73181106812493	39.0	35.0	41.0	21.0	41.0
42-43	34.575647840583194	39.0	35.0	41.0	18.5	41.0
44-45	34.46639147277987	39.0	35.0	41.0	17.5	41.0
46-47	34.3192492136084	39.0	34.0	41.0	15.0	41.0
48-49	34.186913055550946	39.0	34.0	41.0	14.5	41.0
50-51	32.51160445642624	37.0	32.0	39.5	11.0	40.0
52-53	32.66883887053861	37.5	32.5	39.5	9.0	40.0
54-55	33.523971344885034	38.0	33.0	40.0	8.0	41.0
56-57	33.5538314314192	38.0	33.0	41.0	7.5	41.0
58-59	33.3887521395239	38.0	33.0	40.5	6.5	41.0
60-61	33.1552066583227	38.0	33.0	40.0	2.0	41.0
62-63	32.87482438290536	37.0	33.0	40.0	2.0	41.0
64-65	32.59634618014688	37.0	32.5	40.0	2.0	41.0
66-67	32.265312101105714	36.0	32.0	39.5	2.0	41.0
68-69	31.906212182749535	36.0	32.0	39.0	2.0	41.0
70-71	31.523454854491654	35.0	32.0	39.0	2.0	40.5
72-73	31.168761034348424	35.0	31.0	37.5	2.0	40.0
74-75	30.769023890141405	35.0	31.0	37.0	2.0	39.0
76-77	30.38230196711868	35.0	31.0	37.0	2.0	39.0
78-79	30.02640731976195	35.0	31.0	36.0	2.0	39.0
80-81	29.67313682695655	35.0	30.0	36.0	2.0	37.0
82-83	29.36179659914129	35.0	30.0	35.0	2.0	37.0
84-85	29.1151273664274	35.0	30.0	35.0	2.0	36.5
86-87	28.880218651053497	34.0	30.0	35.0	2.0	36.0
88-89	28.67045607604065	34.0	29.5	35.0	2.0	36.0
90-91	28.41034501247451	34.0	29.0	35.0	2.0	35.5
92-93	28.2054238225884	34.0	29.0	35.0	2.0	35.0
94-95	27.987877369535667	34.0	29.0	35.0	2.0	35.0
96-97	27.723242611648956	34.0	29.0	35.0	2.0	35.0
98-99	27.475814573214194	34.0	29.0	35.0	2.0	35.0
100-101	26.42758190802016	33.0	26.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	164614.0
3	26648.0
4	17913.0
5	13273.0
6	12181.0
7	12363.0
8	13129.0
9	13716.0
10	14864.0
11	15964.0
12	16083.0
13	16334.0
14	17231.0
15	17781.0
16	18328.0
17	18732.0
18	17647.0
19	17289.0
20	17319.0
21	18440.0
22	20162.0
23	23158.0
24	27067.0
25	32115.0
26	37709.0
27	44429.0
28	52581.0
29	61703.0
30	73564.0
31	88739.0
32	109465.0
33	141304.0
34	194649.0
35	277488.0
36	446432.0
37	778561.0
38	823272.0
39	148413.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.08311833711493	18.03848291930094	13.135169666679191	24.743229076904935
2	15.761629362614668	19.40448291395109	39.866142911867264	24.967744811566977
3	19.224157747576818	23.64124850239888	29.501530449214254	27.633063300810047
4	13.368532825487527	15.69250793530668	36.91392962192252	34.02502961728327
5	14.12457958704291	37.497648175320954	34.435067092439795	13.942705145196344
6	33.441158648033245	35.285743560727646	17.192230877781483	14.080866913457621
7	28.86081091303622	32.1448408360112	20.79893389816282	18.19541435278976
8	26.693036031568624	35.042626759749645	19.82052354252131	18.443813666160423
9	27.02697451104699	15.70877880859489	19.38619560607478	37.87805107428334
10-11	25.695877774388094	25.31046396169698	27.824592152725014	21.169066111189917
12-13	26.154439290111558	22.75834452638866	27.60962716761808	23.4775890158817
14-15	23.282624297863183	24.464286015078862	24.88568148167632	27.367408205381636
16-17	23.490595773529545	27.47616474474483	25.83238620206472	23.200853279660908
18-19	23.50759049024043	26.956305907871545	27.01544265898524	22.520660942902786
20-21	24.072018819691134	26.1240325844209	27.012809313565523	22.791139282322444
22-23	23.908236200846236	25.96325011344397	26.640493135959392	23.488020549750395
24-25	23.663015357473583	26.07770977255256	26.65570595828824	23.603568911685617
26-27	23.483152807105437	26.343498225053224	26.59343270340569	23.579916264435653
28-29	23.570972501037286	26.704706274475615	26.322384380789188	23.40193684369791
30-31	24.03889072675692	26.20439003779388	26.281718659046582	23.47500057640262
32-33	23.70677266603709	26.282845947872357	26.179912853347602	23.830468532742945
34-35	24.025006968399214	26.434894427456634	26.262562547211555	23.2775360569326
36-37	24.026176705845298	26.222177957461625	26.37768455124167	23.373960785451413
38-39	23.882661991507103	26.36334859339815	26.271350426367402	23.48263898872734
40-41	24.051790849825576	26.115874061251994	26.30676766322737	23.525567425695062
42-43	23.824066054801975	26.14859630430403	26.154982875680975	23.87235476521302
44-45	23.743174990562142	26.215251710202374	26.361559615799646	23.680013683435842
46-47	23.901978895433277	26.145022093644556	26.07700271389668	23.875996297025488
48-49	23.713301996246816	26.233540891783232	26.240133619537314	23.813023492432638
50-51	23.823399979054116	26.292652419918007	26.29424866761983	23.589698933408048
52-53	24.070934052848827	26.07454590642535	26.21449063195757	23.64002940876825
54-55	23.444866911269724	25.96671136765693	26.658614172398565	23.929807548674784
56-57	23.577835363657424	26.14144969747944	26.36238737817893	23.9183275606842
58-59	23.723636884687586	26.06566066652268	26.47261984366953	23.738082605120198
60-61	23.81504518279497	26.10737436171676	26.560149839179882	23.51743061630838
62-63	23.7435208193669	26.05413600000519	26.593886547900347	23.608456632727563
64-65	23.45779884507704	25.855983780717796	26.61003243596849	24.076184938236672
66-67	23.48336239347409	26.003814265143987	26.45623101546536	24.05659232591656
68-69	23.618412164376323	25.908700210854345	26.58323425675173	23.889653368017598
70-71	23.492731477829036	25.718729368483555	26.51459984719702	24.273939306490387
72-73	23.450996585050106	25.993483617428026	26.409880152336147	24.14563964518572
74-75	23.436231518450924	26.030110832196566	26.5356867007593	23.99797094859321
76-77	23.473643013306702	25.64419625153383	26.583337139796154	24.298823595363313
78-79	23.336323966576558	25.706413792011322	26.671321358858595	24.285940882553522
80-81	23.566055701487073	25.836317411112127	26.42465617387681	24.172970713523988
82-83	23.537271466921446	25.842048009471046	26.605209593586277	24.015470930021237
84-85	23.414443364465797	25.908324350818603	26.509867266470195	24.16736501824541
86-87	23.29385744147977	26.040664422635384	26.460125255322716	24.205352880562135
88-89	23.158543317288643	25.74771650075844	26.622259450381502	24.47148073157141
90-91	23.267727325188222	25.65189162915492	26.736018115208708	24.344362930448145
92-93	23.403411149007	25.424731804024464	26.85387262914498	24.31798441782356
94-95	23.246276413478753	25.465061215893552	26.851580981555184	24.43708138907251
96-97	23.141936504201915	25.20559019389245	26.985350619348008	24.667122682557626
98-99	22.882851746683418	25.20058013968066	26.94360879754743	24.972959316088488
100-101	23.140677542237643	25.173543168368102	27.041779425470896	24.643999863923355
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	29.5
2	40.5
3	64.5
4	80.5
5	98.0
6	148.5
7	275.0
8	413.5
9	456.0
10	425.0
11	454.0
12	546.5
13	637.0
14	714.5
15	812.5
16	917.0
17	1039.0
18	1162.0
19	1280.5
20	1431.5
21	1587.5
22	1749.0
23	1942.5
24	2222.5
25	2703.0
26	3575.0
27	5055.5
28	7381.0
29	11180.5
30	16845.0
31	24956.0
32	35734.0
33	44291.5
34	51723.0
35	61826.5
36	74170.5
37	86108.0
38	96828.0
39	107330.5
40	115725.5
41	123196.0
42	131172.5
43	139417.5
44	146659.5
45	154524.0
46	159783.5
47	163420.5
48	168338.0
49	170801.5
50	170284.0
51	167891.0
52	164221.5
53	159614.5
54	154623.0
55	145989.5
56	133855.5
57	119631.0
58	106043.0
59	91177.5
60	74074.0
61	60589.0
62	49995.5
63	38157.5
64	26956.5
65	19667.0
66	14538.0
67	10917.5
68	8111.5
69	5975.0
70	4569.0
71	3356.0
72	2363.5
73	1728.0
74	1316.0
75	1003.5
76	711.0
77	448.0
78	290.5
79	229.0
80	186.5
81	142.0
82	116.0
83	95.0
84	74.5
85	69.5
86	56.5
87	46.5
88	46.0
89	32.0
90	29.0
91	31.0
92	24.5
93	19.5
94	20.0
95	16.0
96	12.5
97	12.0
98	8.5
99	6.5
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0537989241251264
2	0.06159549425592229
3	0.05183035492266631
4	0.05913478275284717
5	0.05092377700048075
6	0.01877911410241533
7	0.016266598146643902
8	0.02305298144986158
9	0.0218355768114981
10-11	0.023946608258873067
12-13	0.029424929131508713
14-15	0.04731041642491256
16-17	0.03135464499444656
18-19	0.02522876846310694
20-21	0.028427693417104587
22-23	0.050224416889080456
24-25	0.04497921605357824
26-27	0.032649756311854516
28-29	0.021641310113886907
30-31	0.013546864380087197
32-33	0.010296134973393233
34-35	0.010050063823085723
36-37	0.0016577424862821808
38-39	0.006967698887654792
40-41	0.0037428717073089866
42-43	0.22897568091772624
44-45	0.20303460123004494
46-47	0.20963966894882552
48-49	0.2057154816570794
50-51	0.20427790809475657
52-53	0.20927703777995124
54-55	0.20813733982063226
56-57	0.21899037266051088
58-59	0.2155065232166835
60-61	0.22125681746597484
62-63	0.21834281700180694
64-65	0.2193141504898629
66-67	0.20798192646254332
68-69	0.2075415886146246
70-71	0.19022595030088027
72-73	0.1972195514148832
74-75	0.19789300929993534
76-77	0.1962352668136532
78-79	0.22392474677983523
80-81	0.23258904149329446
82-83	0.23135868574175686
84-85	0.22648906718830294
86-87	0.24396011886013627
88-89	0.24208220744989473
90-91	0.24147350513071297
92-93	0.24547539910150357
94-95	0.25683352535517134
96-97	0.2571832054108715
98-99	0.2468611682111301
100-101	0.25645794307312303
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3860672.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.22700058383667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.69819630867065	42.56282528043008
2	13.059157538912189	14.685545171296615
3	4.5437868991428525	7.664505258928039
4	2.142526912583811	4.818714478589429
5	1.2027278670833539	3.381289024234619
6	0.7297296425019766	2.461830542100094
7	0.5033721657404189	1.9812174939881566
8	0.36022713675163387	1.6203593142758346
9	0.2673741693053688	1.3530282816272234
>10	1.403514691572789	13.83735491087061
>50	0.066053056943002	2.5112962869155693
>100	0.021387584053289645	2.017498793764677
>500	0.001158349288379693	0.46115002513261477
>1k	7.876774502934428E-4	0.6433851378464814
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.5902226348159078E-5	0.0	2.5902226348159078E-5	0.0
3	0.0	2.5902226348159078E-5	0.0	2.5902226348159078E-5	0.0
4	0.0	2.5902226348159078E-5	0.0	2.5902226348159078E-5	2.5902226348159078E-5
5	2.5902226348159078E-5	2.5902226348159078E-5	0.0	2.5902226348159078E-5	2.5902226348159078E-5
6	5.1804452696318156E-5	2.5902226348159078E-5	0.0	2.5902226348159078E-5	2.5902226348159078E-5
7	5.1804452696318156E-5	2.5902226348159078E-5	0.0	2.5902226348159078E-5	2.5902226348159078E-5
8	5.1804452696318156E-5	2.5902226348159078E-5	0.0	2.5902226348159078E-5	2.5902226348159078E-5
9	5.1804452696318156E-5	2.5902226348159078E-5	0.0	5.1804452696318156E-5	5.1804452696318156E-5
10-11	5.1804452696318156E-5	2.5902226348159078E-5	0.0	7.770667904447723E-5	5.1804452696318156E-5
12-13	5.1804452696318156E-5	2.5902226348159078E-5	0.0	1.0360890539263631E-4	6.475556587039769E-5
14-15	5.1804452696318156E-5	2.5902226348159078E-5	0.0	1.0360890539263631E-4	9.065779221855677E-5
16-17	7.770667904447723E-5	2.5902226348159078E-5	0.0	1.0360890539263631E-4	1.295111317407954E-4
18-19	7.770667904447723E-5	2.5902226348159078E-5	0.0	1.2951113174079537E-4	1.295111317407954E-4
20-21	7.770667904447723E-5	2.5902226348159078E-5	0.0	1.68364471263034E-4	1.295111317407954E-4
22-23	7.770667904447723E-5	2.5902226348159078E-5	0.0	1.9426669761119308E-4	1.8131558443711354E-4
24-25	7.770667904447723E-5	2.5902226348159078E-5	0.0	3.237778293519885E-4	2.3312003713343168E-4
26-27	7.770667904447723E-5	2.5902226348159078E-5	0.0	5.180445269631815E-4	2.3312003713343168E-4
28-29	7.770667904447723E-5	2.5902226348159078E-5	0.0	0.00196856920246009	2.460711503075112E-4
30-31	7.770667904447723E-5	2.5902226348159078E-5	0.0	0.008832659184722244	2.590222634815908E-4
32-33	7.770667904447723E-5	2.5902226348159078E-5	0.0	0.025746812990070124	2.849244898297498E-4
34-35	1.0360890539263631E-4	2.5902226348159078E-5	0.0	0.050302123568124926	2.849244898297498E-4
36-37	1.0360890539263631E-4	2.5902226348159078E-5	0.0	0.08934972978797473	2.849244898297498E-4
38-39	1.0360890539263631E-4	2.5902226348159078E-5	0.0	0.1561386204267029	2.849244898297498E-4
40-41	1.0360890539263631E-4	2.5902226348159078E-5	0.0	0.23099605457288264	2.849244898297498E-4
42-43	1.0360890539263631E-4	2.5902226348159078E-5	0.0	0.3100885027269864	2.9787560300382934E-4
44-45	1.295111317407954E-4	2.5902226348159078E-5	0.0	0.40860762064220946	3.108267161779089E-4
46-47	1.295111317407954E-4	2.5902226348159078E-5	0.0	0.5227587321585465	3.237778293519885E-4
48-49	1.295111317407954E-4	2.5902226348159078E-5	0.0	0.6467138363476618	3.626311688742271E-4
50-51	1.295111317407954E-4	2.5902226348159078E-5	0.0	0.77928143079754	3.626311688742271E-4
52-53	1.295111317407954E-4	2.5902226348159078E-5	0.0	0.9175993194967094	4.7919118744094293E-4
54-55	1.295111317407954E-4	2.5902226348159078E-5	0.0	1.0767296470666246	6.216534323558178E-4
56-57	1.295111317407954E-4	2.5902226348159078E-5	0.0	1.2588611516337052	6.47555658703977E-4
58-59	1.295111317407954E-4	2.5902226348159078E-5	0.0	1.4524802935861942	6.47555658703977E-4
60-61	1.295111317407954E-4	2.5902226348159078E-5	0.0	1.6608248512176118	6.47555658703977E-4
62-63	1.295111317407954E-4	2.5902226348159078E-5	0.0	1.886213073786118	6.73457885052136E-4
64-65	1.295111317407954E-4	2.5902226348159078E-5	0.0	2.1316366684349255	6.73457885052136E-4
66-67	1.5541335808895446E-4	2.5902226348159078E-5	0.0	2.4171569094706826	6.99360111400295E-4
68-69	1.8131558443711354E-4	2.5902226348159078E-5	0.0	2.7308976261127595	7.252623377484542E-4
70-71	2.3312003713343168E-4	3.8853339522238614E-5	0.0	3.058262913813968	7.641156772706928E-4
72-73	2.590222634815908E-4	5.1804452696318156E-5	0.0	3.4064794937254446	8.029690167929314E-4
74-75	2.590222634815908E-4	5.1804452696318156E-5	0.0	3.7704446272566017	8.029690167929314E-4
76-77	2.590222634815908E-4	5.1804452696318156E-5	0.0	4.177912549939492	8.029690167929314E-4
78-79	2.590222634815908E-4	5.1804452696318156E-5	0.0	4.639826952406214	8.029690167929314E-4
80-81	3.108267161779089E-4	5.1804452696318156E-5	0.0	5.117451055153092	8.806756958374086E-4
82-83	3.36728942526068E-4	5.1804452696318156E-5	0.0	5.615719232299455	9.84284601230045E-4
84-85	3.36728942526068E-4	5.1804452696318156E-5	0.0	6.14095162707425	9.972357144041244E-4
86-87	3.36728942526068E-4	5.1804452696318156E-5	0.0	6.707588730666578	0.001010186827578204
88-89	3.36728942526068E-4	5.1804452696318156E-5	0.0	7.315591689736916	0.0010231379407522837
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3045	0.0	48.618446	1
GTATCAA	5675	0.0	41.638706	1
TCAACGC	6605	0.0	35.56543	4
CAACGCA	6665	0.0	35.24252	5
ATCAACG	6945	0.0	33.755516	3
TATCAAC	7080	0.0	33.045273	2
AACGCAG	7240	0.0	32.497314	6
GTACATG	11395	0.0	28.857098	1
TACATGG	11775	0.0	27.365574	2
ACGCAGA	8575	0.0	26.503042	7
ACATGGG	12520	0.0	25.244104	3
CGCAGAG	9045	0.0	24.287863	8
CATGGGG	10305	0.0	22.795696	4
GTATAGG	1205	0.0	19.688686	1
GCAGAGT	10455	0.0	19.650822	9
ATGGGGG	6860	0.0	19.299318	5
TAGCCCT	2220	0.0	18.384048	4
GAGTACA	8950	0.0	18.29068	1
GTATAGC	1000	0.0	17.081903	1
AGAGTAC	8900	0.0	16.95373	10-11
>>END_MODULE