FastQCFastQC Report
Mon 15 Aug 2016
SRR2921383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3860672
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.049.1197971
GTATCAA57200.042.4637871
TCAACGC69400.034.1923264
ATCAACG71900.033.1354563
CAACGCA71600.033.074725
TATCAAC74850.032.0831382
AACGCAG76850.030.7501536
GTACATG115550.025.4632931
ACGCAGA92550.025.0196747
TACATGG117200.024.4178712
ACATGGG118350.023.8197023
CGCAGAG96850.023.3197088
CATGGGG99300.021.5070744
TAAGGTG18550.019.4827185
GCAGAGT111150.019.3356199
ATGGGGG64500.018.6526575
AGAGTAC92000.018.21798710-11
TAAGGCG13200.018.012585
GTACACT37750.016.8463544
GAGTACA87550.016.7220081
Produced by FastQC (version 0.11.5)