##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921383_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3860672
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.468026550818095	33.0	31.0	34.0	30.0	34.0
2	31.599524383319796	34.0	31.0	34.0	29.0	34.0
3	31.67651149851632	34.0	31.0	34.0	29.0	34.0
4	35.1722948750891	37.0	35.0	37.0	33.0	37.0
5	34.92772993924374	37.0	35.0	37.0	32.0	37.0
6	34.88520210989175	37.0	35.0	37.0	32.0	37.0
7	34.75587851026972	37.0	35.0	37.0	32.0	37.0
8	34.71688892503688	37.0	35.0	37.0	32.0	37.0
9	36.339875026938316	39.0	37.0	39.0	32.0	39.0
10-11	36.19464810789417	39.0	37.0	39.0	32.0	39.0
12-13	36.13106150949886	39.0	37.0	39.0	32.0	39.0
14-15	37.38417353248346	40.0	38.0	41.0	32.0	41.0
16-17	37.364057993012615	40.0	38.0	41.0	32.0	41.0
18-19	37.32612703177064	40.0	38.0	41.0	31.5	41.0
20-21	37.29241593173416	40.0	38.0	41.0	31.0	41.0
22-23	37.18694595137841	40.0	38.0	41.0	31.0	41.0
24-25	37.121841223496844	40.0	37.5	41.0	31.0	41.0
26-27	36.99020882892927	40.0	37.0	41.0	30.5	41.0
28-29	36.8436280005139	40.0	37.0	41.0	30.0	41.0
30-31	36.71521680681498	40.0	37.0	41.0	30.0	41.0
32-33	36.67029211494786	40.0	37.0	41.0	30.0	41.0
34-35	36.80039045015997	40.0	37.0	41.0	30.0	41.0
36-37	36.77823614645326	40.0	37.0	41.0	30.0	41.0
38-39	36.699169211992114	40.0	37.0	41.0	30.0	41.0
40-41	36.58430332853969	40.0	37.0	41.0	30.0	41.0
42-43	36.49358246439998	40.0	37.0	41.0	30.0	41.0
44-45	36.374613927316275	40.0	36.5	41.0	29.5	41.0
46-47	36.23744739257829	40.0	36.0	41.0	29.0	41.0
48-49	36.09537368105863	40.0	36.0	41.0	28.5	41.0
50-51	35.95495253157999	40.0	35.5	41.0	28.0	41.0
52-53	35.796102466099164	39.5	35.0	41.0	28.0	41.0
54-55	35.59930602755168	39.0	35.0	41.0	28.0	41.0
56-57	35.34568165852991	39.0	35.0	41.0	27.0	41.0
58-59	35.130394397659266	39.0	35.0	41.0	26.5	41.0
60-61	34.865808076935835	38.0	35.0	40.0	26.0	41.0
62-63	34.57713993833198	38.0	34.0	40.0	26.0	41.0
64-65	34.273621664829335	37.0	34.0	40.0	26.0	41.0
66-67	33.94576669553902	37.0	34.0	39.5	26.0	41.0
68-69	33.58290447880576	36.0	34.0	39.0	25.0	41.0
70-71	33.20057233559339	36.0	33.5	39.0	24.0	40.5
72-73	32.80347087242843	35.0	33.0	38.0	23.0	40.0
74-75	32.393429434046716	35.0	33.0	37.0	22.0	39.0
76-77	29.61018017070603	33.0	29.0	34.5	17.5	36.5
78-79	31.266599830288612	34.5	31.5	36.0	20.0	38.0
80-81	31.45607642918124	35.0	32.0	36.0	20.0	37.0
82-83	31.28040908422161	35.0	32.0	35.5	20.0	37.0
84-85	31.041078988321203	35.0	32.0	35.0	19.5	36.5
86-87	30.789047347197588	35.0	32.0	35.0	18.0	36.0
88-89	30.503669568406742	35.0	32.0	35.0	13.5	36.0
90-91	30.317059439392935	35.0	32.0	35.0	9.0	35.5
92-93	30.169176376547917	34.0	32.0	35.0	7.0	35.0
94-95	30.069602390464667	34.0	32.0	35.0	2.0	35.0
96-97	29.878906703288962	34.0	31.5	35.0	2.0	35.0
98-99	29.61797920154833	34.0	31.0	35.0	2.0	35.0
100-101	28.543348023349303	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65373.0
3	21853.0
4	12069.0
5	8677.0
6	7481.0
7	7637.0
8	8188.0
9	8437.0
10	9525.0
11	10481.0
12	11337.0
13	12123.0
14	13669.0
15	14366.0
16	15174.0
17	16163.0
18	15291.0
19	14129.0
20	13764.0
21	13507.0
22	14399.0
23	16325.0
24	19405.0
25	23164.0
26	28201.0
27	33847.0
28	40887.0
29	49430.0
30	60030.0
31	75116.0
32	94868.0
33	125218.0
34	181191.0
35	267466.0
36	459299.0
37	902810.0
38	1024001.0
39	145768.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.65228665350651	19.678884480549417	13.884219819471106	25.784609046472973
2	14.686937110785664	20.651846755815452	38.74638598250766	25.91483015089122
3	17.715984415203597	24.75227494146376	29.50630616441852	28.02543447891413
4	12.911057762047056	16.885747234575167	36.03698768665841	34.16620731671937
5	13.776446254546812	37.810711184890145	34.64030865590571	13.772533904657328
6	33.26809349880214	35.960013749062	17.00161657678921	13.770276175346646
7	28.591920975251927	32.305712178008754	20.97947399721913	18.122892849520188
8	26.48622815913091	35.77606560646739	19.587861456588946	18.149844777812746
9	26.5694745809731	15.852347946768838	19.434549071910038	38.14362840034802
10-11	25.590201782387535	25.679052778113792	27.849750152763292	20.880995286735374
12-13	25.984361492360442	22.888001735453436	27.824732154131578	23.302904618054544
14-15	23.02916954575862	24.64259648654722	25.027279868460845	27.300954099233316
16-17	23.319691913460066	27.787441475485398	25.848722831292044	23.044143779762493
18-19	23.338838000255656	27.17440926928752	27.08324618294239	22.403506547514436
20-21	23.894997392924036	26.233530286503793	27.052292012193814	22.81918030837836
22-23	23.725483796686632	26.06451330484598	26.730008151453855	23.47999474701353
24-25	23.502407519220395	26.248396303903032	26.6825631059109	23.566633070965672
26-27	23.311161596204474	26.522443525144308	26.705319235325682	23.461075643325536
28-29	23.411489711627144	26.836263015647464	26.41081504087075	23.34143223185464
30-31	23.853608752459955	26.350486732077222	26.350007467460845	23.445897048001978
32-33	23.549592829530365	26.419565633019644	26.21035871518896	23.820482822261035
34-35	23.841890206987166	26.56477360835254	26.349810151881087	23.243526032779208
36-37	23.841922187573417	26.380553705690996	26.475783956195897	23.30174015053969
38-39	23.798169978251156	26.467334833161495	26.320507777870883	23.413987410716473
40-41	23.969316812613336	26.25419782045548	26.28915456359051	23.48733080334067
42-43	23.711911170771778	26.241318470781543	26.175365918798576	23.871404439648106
44-45	23.616284625669678	26.281360413673355	26.37437034375595	23.727984616901022
46-47	23.76911364991947	26.241721007253975	26.141780523392306	23.847384819434254
48-49	23.566010818630055	26.394120708472347	26.278958072821744	23.76091040007585
50-51	23.718731061908606	26.352407192909723	26.31697082237118	23.611890922810495
52-53	23.97445114474978	26.19027051081521	26.184468309164615	23.650810035270393
54-55	23.300436352747912	26.10190336415898	26.60582266454405	23.99183761854906
56-57	23.484045760169554	26.218532824633723	26.316086686164486	23.981334729032234
58-59	23.600897129326043	26.158591285214438	26.42925586603988	23.811255719419645
60-61	23.66754801272309	26.225040365165842	26.517161639143062	23.59024998296801
62-63	23.609406290722468	26.231835998725376	26.42371572836058	23.73504198219157
64-65	23.296931115973493	25.98869053718977	26.515413473488756	24.198964873347983
66-67	23.352993099280933	26.183165033592793	26.305676344446976	24.158165522679298
68-69	23.494210960091678	26.098546981685505	26.38589384812654	24.021348210096278
70-71	23.39186340337654	25.895562156742148	26.33984012471397	24.372734315167342
72-73	23.3381787345484	26.20516994507437	26.148209168469823	24.308442151907407
74-75	23.310696453575655	26.322183472853943	26.171744494947735	24.195375578622663
76-77	23.29950188917547	25.884066867282012	26.329764789799203	24.486666453743307
78-79	23.140094633613895	26.034184380930558	26.397811884425632	24.42790910102992
80-81	23.444600475331967	26.12378197965488	26.094366910613047	24.337250634400107
82-83	23.398059296272418	26.16981034555791	26.22317976283512	24.208950595334557
84-85	23.25883856671765	26.20565364597436	26.094872780139816	24.440635007168172
86-87	23.111729240372856	26.39583618220786	25.99353081353487	24.498903763884417
88-89	22.979092633323646	26.162776353273028	26.11679484612362	24.74133616727971
90-91	22.996551036410633	26.10628316842103	26.19481674498133	24.702349050187
92-93	23.126822550331674	25.926824830949226	26.26589823677164	24.680454381947463
94-95	22.95278218642233	25.97548330242605	26.268802433692677	24.80293207745894
96-97	22.81211377347733	25.82575246738114	26.305330693446855	25.056803065694677
98-99	22.54572299084666	25.812199489070707	26.245006459467245	25.397071060615385
100-101	22.604807228676922	25.921123880637413	26.23718681710351	25.236882073582155
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	15.0
2	22.5
3	29.0
4	36.0
5	43.0
6	64.5
7	157.5
8	241.0
9	273.5
10	261.0
11	238.5
12	255.0
13	289.5
14	331.0
15	359.5
16	410.5
17	473.0
18	564.0
19	651.0
20	727.0
21	859.5
22	1079.0
23	1362.0
24	1711.0
25	2269.5
26	3258.5
27	4929.5
28	7593.5
29	11676.0
30	17626.5
31	25972.0
32	37146.0
33	46094.5
34	53461.5
35	63164.0
36	75535.0
37	88032.5
38	98519.5
39	108612.5
40	116913.5
41	124757.5
42	132660.0
43	139986.0
44	147125.0
45	155531.0
46	161136.0
47	164810.0
48	169926.0
49	172594.0
50	171656.5
51	168492.0
52	164539.5
53	160424.5
54	155581.0
55	146553.5
56	133686.0
57	118586.0
58	104544.0
59	89407.0
60	71893.5
61	58916.5
62	48534.5
63	36255.0
64	25115.5
65	17900.5
66	13182.5
67	9844.5
68	7276.0
69	5338.5
70	3945.5
71	2875.5
72	2020.5
73	1416.5
74	1033.0
75	735.5
76	481.0
77	258.5
78	133.0
79	73.5
80	48.5
81	38.0
82	27.0
83	19.0
84	10.0
85	7.0
86	5.5
87	4.5
88	4.5
89	5.0
90	4.0
91	2.5
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15823670076090382
2	2.590222634815908E-4
3	0.006294241002602655
4	0.0037299205941349074
5	0.02846654675662683
6	0.0011656001856671584
7	2.072178107852726E-4
8	0.0019944714288082487
9	2.5902226348159075E-5
10-11	0.1091908351706646
12-13	2.460711503075112E-4
14-15	0.0058150498151617125
16-17	9.065779221855677E-5
18-19	2.2016892395935217E-4
20-21	6.47555658703977E-4
22-23	2.849244898297498E-4
24-25	0.0015282313545413855
26-27	0.0032766316330421234
28-29	0.007006552227177031
30-31	0.015321166884936092
32-33	0.01600757588316231
34-35	0.012458970873464517
36-37	7.511645640966132E-4
38-39	1.9426669761119308E-4
40-41	0.004830765213931668
42-43	0.008314614657759064
44-45	0.008586588034414734
46-47	0.009700383767385573
48-49	0.012407166420768197
50-51	0.005996365399598826
52-53	0.0017743025048488968
54-55	8.806756958374087E-4
56-57	0.006229485436732259
58-59	0.009298899258989109
60-61	0.007485743414617973
62-63	0.018183362896407672
64-65	0.03464422774066277
66-67	0.037208548149130515
68-69	0.02722323989191519
70-71	0.04148241549657676
72-73	0.02586337300863684
74-75	0.025060403991843908
76-77	0.24661509706082255
78-79	0.07098505130712994
80-81	0.05453713757604894
82-83	0.020294394343782638
84-85	0.032403685161547006
86-87	0.08170857301526781
88-89	0.2644876332410523
90-91	0.3215631889992209
92-93	0.05356580408799297
94-95	0.005206347495979975
96-97	0.010490401671004426
98-99	0.0015411824677154652
100-101	0.0012562579778857153
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3860672.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.4743971669298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.19303590796123	40.416209050705646
2	13.745004989922204	14.975017217649084
3	4.850548998130202	7.926921978053944
4	2.3480763194805956	5.116401680225949
5	1.2472180646189575	3.3970726102911297
6	0.80583630306132	2.6338468094695653
7	0.5286952881687967	2.0160249975594007
8	0.389801683739174	1.6987369389076583
9	0.28336754377243933	1.3892648511309504
>10	1.5078274951786863	14.383628852935699
>50	0.07467158589519153	2.743844595987209
>100	0.023909371264925608	2.1774216784486486
>500	0.0010987696059618246	0.4328283566028521
>1k	9.076792002964246E-4	0.6927803820323584
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.1804452696318156E-5
2	0.0	0.0	0.0	2.5902226348159078E-5	5.1804452696318156E-5
3	0.0	0.0	0.0	2.5902226348159078E-5	5.1804452696318156E-5
4	0.0	0.0	0.0	2.5902226348159078E-5	1.0360890539263631E-4
5	0.0	0.0	0.0	2.5902226348159078E-5	1.0360890539263631E-4
6	2.5902226348159078E-5	0.0	0.0	2.5902226348159078E-5	1.0360890539263631E-4
7	2.5902226348159078E-5	0.0	0.0	2.5902226348159078E-5	1.0360890539263631E-4
8	2.5902226348159078E-5	0.0	0.0	2.5902226348159078E-5	1.0360890539263631E-4
9	2.5902226348159078E-5	0.0	0.0	5.1804452696318156E-5	1.0360890539263631E-4
10-11	2.5902226348159078E-5	0.0	0.0	1.0360890539263631E-4	1.0360890539263631E-4
12-13	2.5902226348159078E-5	0.0	0.0	1.295111317407954E-4	1.0360890539263631E-4
14-15	2.5902226348159078E-5	0.0	0.0	1.295111317407954E-4	1.0360890539263631E-4
16-17	5.1804452696318156E-5	0.0	0.0	1.5541335808895446E-4	1.0360890539263631E-4
18-19	5.1804452696318156E-5	0.0	0.0	1.5541335808895446E-4	1.0360890539263631E-4
20-21	5.1804452696318156E-5	0.0	0.0	1.68364471263034E-4	1.0360890539263631E-4
22-23	5.1804452696318156E-5	0.0	0.0	2.0721781078527262E-4	1.4246224491487494E-4
24-25	5.1804452696318156E-5	0.0	0.0	3.496800557001475E-4	2.0721781078527262E-4
26-27	6.475556587039769E-5	0.0	0.0	5.30995640137261E-4	2.3312003713343168E-4
28-29	7.770667904447723E-5	0.0	0.0	0.002020373655156408	2.3312003713343168E-4
30-31	7.770667904447723E-5	0.0	0.0	0.009169388127248313	2.3312003713343168E-4
32-33	7.770667904447723E-5	0.0	0.0	0.026964217628433595	2.3312003713343168E-4
34-35	1.0360890539263631E-4	0.0	0.0	0.05227069277058502	2.849244898297498E-4
36-37	1.295111317407954E-4	0.0	0.0	0.09236733915753527	2.849244898297498E-4
38-39	1.295111317407954E-4	2.5902226348159078E-5	0.0	0.1604513411136714	2.9787560300382934E-4
40-41	1.295111317407954E-4	2.5902226348159078E-5	0.0	0.2366038865772591	3.108267161779089E-4
42-43	1.295111317407954E-4	2.5902226348159078E-5	0.0	0.3170562016146412	3.108267161779089E-4
44-45	1.295111317407954E-4	2.5902226348159078E-5	0.0	0.41798422658024303	3.237778293519885E-4
46-47	1.295111317407954E-4	2.5902226348159078E-5	0.0	0.534065053959518	3.36728942526068E-4
48-49	1.295111317407954E-4	2.5902226348159078E-5	0.0	0.6601829940487045	3.626311688742271E-4
50-51	1.8131558443711354E-4	2.5902226348159078E-5	0.0	0.7943176731926462	3.626311688742271E-4
52-53	1.8131558443711354E-4	2.5902226348159078E-5	0.0	0.933658699832568	4.4033784791870433E-4
54-55	1.8131558443711354E-4	2.5902226348159078E-5	0.0	1.0949389121893804	5.309956401372612E-4
56-57	1.8131558443711354E-4	2.5902226348159078E-5	0.0	1.2787929148086137	5.568978664854201E-4
58-59	1.8131558443711354E-4	2.5902226348159078E-5	0.0	1.4744842348689553	5.828000928335792E-4
60-61	1.8131558443711354E-4	2.5902226348159078E-5	0.0	1.6857039396250189	5.957512060076588E-4
62-63	2.0721781078527262E-4	2.5902226348159078E-5	0.0	1.9133456558858146	5.957512060076588E-4
64-65	2.0721781078527262E-4	2.5902226348159078E-5	0.0	2.161566690980223	5.957512060076588E-4
66-67	2.0721781078527262E-4	2.5902226348159078E-5	0.0	2.4496383013112744	6.087023191817384E-4
68-69	2.0721781078527262E-4	2.5902226348159078E-5	0.0	2.7656584138719893	6.216534323558178E-4
70-71	2.0721781078527262E-4	2.5902226348159078E-5	0.0	3.097336422260166	6.605067718780564E-4
72-73	2.460711503075112E-4	2.5902226348159078E-5	0.0	3.4515493675712414	7.511645640966132E-4
74-75	2.719733766556703E-4	2.5902226348159078E-5	0.0	3.822676466687665	7.511645640966132E-4
76-77	2.9787560300382934E-4	2.5902226348159078E-5	0.0	4.238148177312136	7.511645640966132E-4
78-79	3.108267161779089E-4	2.5902226348159078E-5	0.0	4.709154261227061	7.511645640966132E-4
80-81	4.1443562157054525E-4	2.5902226348159078E-5	0.0	5.19706154783416	7.770667904447722E-4
82-83	4.5328896109278385E-4	2.5902226348159078E-5	0.0	5.7046027220131625	9.065779221855677E-4
84-85	4.6624007426686337E-4	2.5902226348159078E-5	0.0	6.240584540722444	9.065779221855677E-4
86-87	5.05093413789102E-4	5.1804452696318156E-5	0.0	6.818696330587007	9.065779221855677E-4
88-89	5.439467533113406E-4	5.1804452696318156E-5	0.0	7.438070367024187	9.065779221855677E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3145	0.0	49.119797	1
GTATCAA	5720	0.0	42.463787	1
TCAACGC	6940	0.0	34.192326	4
ATCAACG	7190	0.0	33.135456	3
CAACGCA	7160	0.0	33.07472	5
TATCAAC	7485	0.0	32.083138	2
AACGCAG	7685	0.0	30.750153	6
GTACATG	11555	0.0	25.463293	1
ACGCAGA	9255	0.0	25.019674	7
TACATGG	11720	0.0	24.417871	2
ACATGGG	11835	0.0	23.819702	3
CGCAGAG	9685	0.0	23.319708	8
CATGGGG	9930	0.0	21.507074	4
TAAGGTG	1855	0.0	19.482718	5
GCAGAGT	11115	0.0	19.335619	9
ATGGGGG	6450	0.0	18.652657	5
AGAGTAC	9200	0.0	18.217987	10-11
TAAGGCG	1320	0.0	18.01258	5
GTACACT	3775	0.0	16.846354	4
GAGTACA	8755	0.0	16.722008	1
>>END_MODULE