FastQCFastQC Report
Mon 15 Aug 2016
SRR2921382_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921382_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452574
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18440.12694706087263025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.12109537965019337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17450.060.370181
GTATCAA29600.037.994571
TCAACGC36550.030.7709474
ATCAACG37950.029.5127753
TATCAAC38350.029.452452
CAACGCA38100.029.3935455
AACGCAG39800.028.2465696
TAACCCG1056.9536334E-527.116085
GTACATG37400.025.2491281
TACATGG38500.023.9139212
ACGCAGA46900.023.2664537
ACATGGG40450.021.9398123
GTGGTAT8050.021.8107591
CGCAGAG48950.021.809718
TGGTATC8450.020.2188112
GTGTAAG7150.019.9104061
GTCTACG1200.00479137119.7721391
GAGTACA30800.019.7207831
CAGAGTA47950.019.6917610-11
GCAGAGT51750.019.5294199
Produced by FastQC (version 0.11.5)