##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921382_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1452574
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.28915291062624	31.0	31.0	34.0	26.0	34.0
2	30.347912739729612	31.0	31.0	34.0	26.0	34.0
3	30.379566204544485	31.0	31.0	34.0	26.0	34.0
4	33.85536984690625	37.0	35.0	37.0	30.0	37.0
5	33.8461104219131	37.0	35.0	37.0	30.0	37.0
6	33.89187676497032	37.0	35.0	37.0	30.0	37.0
7	33.80877256511544	37.0	35.0	37.0	29.0	37.0
8	33.77242811725943	37.0	35.0	37.0	29.0	37.0
9	35.30766418784861	39.0	35.0	39.0	29.0	39.0
10-11	35.205487982023634	39.0	35.0	39.0	28.5	39.0
12-13	35.0976032890579	39.0	35.0	39.0	27.5	39.0
14-15	36.201479236169725	40.0	36.0	41.0	27.0	41.0
16-17	36.143617468025724	40.0	36.0	41.0	27.0	41.0
18-19	36.104893795427984	40.0	36.0	41.0	27.0	41.0
20-21	36.049166514064	40.0	36.0	41.0	27.0	41.0
22-23	35.92690458455128	40.0	36.0	41.0	27.0	41.0
24-25	35.82409708558738	40.0	36.0	41.0	27.0	41.0
26-27	35.660044514083275	40.0	36.0	41.0	26.5	41.0
28-29	35.51042287690679	40.0	36.0	41.0	25.5	41.0
30-31	35.335975998468925	39.0	35.0	41.0	25.0	41.0
32-33	35.123229177997125	39.0	35.0	41.0	24.5	41.0
34-35	34.93757598580176	39.0	35.0	41.0	24.0	41.0
36-37	34.747067619274475	39.0	35.0	41.0	23.5	41.0
38-39	34.954514881857996	39.0	35.0	41.0	24.0	41.0
40-41	35.04648472298141	40.0	35.0	41.0	23.5	41.0
42-43	34.85176039224163	40.0	35.0	41.0	22.0	41.0
44-45	34.69860296274062	39.5	35.0	41.0	20.5	41.0
46-47	34.497343680941555	39.0	35.0	41.0	18.5	41.0
48-49	34.31248666160898	39.0	34.0	41.0	15.5	41.0
50-51	32.59133338473634	37.0	32.0	39.5	12.0	40.0
52-53	32.73636799226752	37.5	32.5	39.5	10.0	40.0
54-55	33.58986667804876	38.0	33.5	40.0	8.5	41.0
56-57	33.60009748212484	38.0	34.0	41.0	8.0	41.0
58-59	33.414318650891445	38.0	33.5	40.5	7.0	41.0
60-61	33.16716015844976	37.5	33.0	40.0	3.5	41.0
62-63	32.87431139480674	37.0	33.0	40.0	2.0	41.0
64-65	32.57395664523804	36.5	33.0	40.0	2.0	41.0
66-67	32.224597507596854	36.0	32.0	39.0	2.0	41.0
68-69	31.852339020249573	35.5	32.0	39.0	2.0	41.0
70-71	31.465937019387653	35.0	32.0	39.0	2.0	40.5
72-73	31.10121928383683	35.0	31.5	37.5	2.0	40.0
74-75	30.694301977042134	35.0	31.0	37.0	2.0	39.0
76-77	30.301887890048974	35.0	31.0	36.5	2.0	39.0
78-79	29.93992078888924	35.0	31.0	36.0	2.0	38.5
80-81	29.58624001255702	35.0	30.0	36.0	2.0	37.0
82-83	29.27451475794004	35.0	30.0	35.0	2.0	37.0
84-85	29.026782456522007	35.0	30.0	35.0	2.0	36.5
86-87	28.7756176277422	34.5	30.0	35.0	2.0	36.0
88-89	28.54644892446099	34.0	29.5	35.0	2.0	36.0
90-91	28.277040963145424	34.0	29.0	35.0	2.0	35.5
92-93	28.067918742866112	34.0	29.0	35.0	2.0	35.0
94-95	27.834860392654694	34.0	29.0	35.0	2.0	35.0
96-97	27.546170453278112	34.0	29.0	35.0	2.0	35.0
98-99	27.271514566555645	34.0	28.0	35.0	2.0	35.0
100-101	26.200844500865358	33.0	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51119.0
3	8624.0
4	6071.0
5	4660.0
6	4315.0
7	4542.0
8	4826.0
9	5163.0
10	5737.0
11	6153.0
12	6247.0
13	6522.0
14	7126.0
15	8099.0
16	8745.0
17	9607.0
18	8551.0
19	7305.0
20	7326.0
21	7552.0
22	8135.0
23	9306.0
24	10691.0
25	12326.0
26	14581.0
27	17053.0
28	19640.0
29	23433.0
30	27500.0
31	33260.0
32	40379.0
33	52405.0
34	71882.0
35	104350.0
36	169546.0
37	295738.0
38	306161.0
39	57887.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.297105816538014	18.762703680769892	13.449870268341687	24.490320234350406
2	16.647918855155076	20.11586216397734	38.311250408996194	24.924968571871396
3	19.424418312322253	23.321575944672084	30.053731335059403	27.200274407946257
4	14.101006343892712	16.179130590512404	35.54694549487184	34.17291757072304
5	14.686335782330168	36.467576333419885	34.11429401874939	14.731793865500553
6	33.49640676136172	34.49564177369723	16.88515751791455	15.122793947026498
7	28.20053444086483	32.14921379631345	21.022625948543023	18.6276258142787
8	26.296617889784123	34.16429163117598	20.26346540114401	19.275625077895885
9	26.453011374551828	15.674489095399844	20.11062627859561	37.76187325145272
10-11	25.445270894645734	25.20977619740791	27.928397217581953	21.41655569036441
12-13	25.82879847677023	23.244231507511994	27.519122145880925	23.407847869836846
14-15	23.434843007354953	24.997090114170835	25.00180791723114	26.56625896124308
16-17	23.124261227025087	27.704558984598403	26.218387919467247	22.952791868909266
18-19	23.118103350330006	26.822884568375173	27.86148342738312	22.197528653911704
20-21	23.496627687701398	26.04466966695554	27.864743920145628	22.59395872519743
22-23	23.564653690998743	25.845187365219434	27.42422499667676	23.165933947105064
24-25	23.340209025637492	26.13650372768126	27.43260042356101	23.090686823120233
26-27	23.057567135030315	26.315086341061654	27.513414610631976	23.113931913276055
28-29	23.095477290050976	26.589606918163206	27.388722523622988	22.926193268162827
30-31	23.605707231395144	26.10935119247206	27.21438195872633	23.070559617406463
32-33	23.28296306088221	26.190200792595103	27.057359870673153	23.469476275849534
34-35	23.687740864988236	26.31716464794769	27.03960039863747	22.955494088426605
36-37	23.554086271194492	26.27339061614709	27.166400814011688	23.006122298646734
38-39	23.407242837678837	26.255751302756565	27.220503725653135	23.116502133911464
40-41	23.553882159850826	26.08827804897023	27.16832049887937	23.18951929229957
42-43	23.47562469250976	26.20223939145741	26.79030908073443	23.531826835298393
44-45	23.321171332446188	26.287340550511622	26.94524754121318	23.446240575829005
46-47	23.58862615413325	26.216015870097554	26.64657263774143	23.548785338027766
48-49	23.260945450732365	26.49063296797907	26.698628265836614	23.549793315451947
50-51	23.37144359070232	26.420114710196017	26.78912170953237	23.419319989569292
52-53	23.609225892242712	26.276821610098605	26.67268020344841	23.441272294210275
54-55	23.108392358579145	26.19337698453489	26.92151407257984	23.776716584306126
56-57	23.215271977858016	26.29342954041867	26.695085842262188	23.79621263946112
58-59	23.206810441269155	26.24440164576005	26.86040603095787	23.688381882012926
60-61	23.25572570042306	26.147598133056142	27.093489673264127	23.503186493256667
62-63	23.31264068442704	26.08463920104182	27.034247225120957	23.568472889410184
64-65	23.033442931958124	26.034934259798764	26.968885650721568	23.962737157521545
66-67	23.07638178405142	26.153605225450338	26.78446880038302	23.985544190115217
68-69	23.04758481667952	26.023498521597915	26.972938964000942	23.95597769772162
70-71	22.976855188872833	25.83024379476957	26.949582179426606	24.243318836930992
72-73	22.944273295798663	26.00922133267848	26.85695897445095	24.189546397071908
74-75	22.927377415458523	26.134824888095455	26.822152306071782	24.115645390374244
76-77	22.990523208430435	25.810348972321506	26.869568486434602	24.329559332813457
78-79	22.814569844860497	25.86260965143032	26.98652495310695	24.336295550602234
80-81	22.933811017558483	25.93567961667497	26.839686892343025	24.290822473423525
82-83	22.82634759462532	25.789391061635627	27.068646376196565	24.315614967542494
84-85	22.86723848965316	25.88162560383307	26.98458479123815	24.26655111527562
86-87	22.79346534004351	25.93122699989821	26.91277170959444	24.36253595046384
88-89	22.662054944546846	25.62380051633437	27.033161474913754	24.680983064205027
90-91	22.707093598846704	25.477496899729275	27.093295203051344	24.722114298372677
92-93	22.729332116416813	25.333268921872925	27.272030876093627	24.665368085616635
94-95	22.694206000437585	25.177294440253412	27.333954050802667	24.79454550850634
96-97	22.698577961246365	25.01296699739556	27.356827266481027	24.93162777487705
98-99	22.579126874911147	25.034057924005626	27.29540745975506	25.09140774132817
100-101	22.659092987502394	24.819040947216035	27.467194431551324	25.05467163373025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	151.0
1	111.0
2	96.0
3	140.0
4	151.0
5	173.0
6	247.5
7	422.0
8	632.5
9	749.0
10	683.0
11	581.5
12	601.0
13	630.5
14	711.5
15	804.0
16	912.5
17	1032.0
18	1117.5
19	1210.5
20	1319.0
21	1531.0
22	1772.0
23	2033.5
24	2401.0
25	2962.5
26	3769.5
27	4851.5
28	6382.0
29	8353.0
30	10689.0
31	13596.0
32	17168.0
33	19507.0
34	21090.5
35	23686.0
36	27199.5
37	30806.5
38	34183.0
39	37269.0
40	39758.5
41	42395.0
42	45089.0
43	48238.5
44	51435.5
45	54463.5
46	56529.5
47	58562.0
48	60839.0
49	61836.5
50	61583.0
51	60478.0
52	59637.0
53	58849.0
54	57588.0
55	54360.5
56	49449.5
57	44180.0
58	39559.5
59	34983.0
60	28803.0
61	23350.5
62	19459.0
63	15110.0
64	10973.5
65	8168.5
66	6188.5
67	4745.0
68	3654.5
69	2796.5
70	2112.0
71	1542.0
72	1088.0
73	798.5
74	631.0
75	466.5
76	333.0
77	220.0
78	154.5
79	113.0
80	68.5
81	57.5
82	44.0
83	33.5
84	37.5
85	32.5
86	22.5
87	18.0
88	11.5
89	8.5
90	9.5
91	9.5
92	7.0
93	3.5
94	7.0
95	8.5
96	6.0
97	4.0
98	3.5
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050324458512957006
2	0.05844796891586935
3	0.04963602542796443
4	0.05397315386341763
5	0.04729535293898968
6	0.01810579013530464
7	0.014801311327340293
8	0.021685642177266012
9	0.020446462624279384
10-11	0.02147911225176824
12-13	0.02729637181995547
14-15	0.04316475442903425
16-17	0.028879767915438385
18-19	0.022649448496255612
20-21	0.026194878883967355
22-23	0.04660691985399711
24-25	0.04123714179105505
26-27	0.029327249420683557
28-29	0.019344969688291268
30-31	0.011772205753372978
32-33	0.008949630104903433
34-35	0.008708678525156034
36-37	0.0010326496274888578
38-39	0.005300934754442803
40-41	0.0026504673772214014
42-43	0.23000549369601825
44-45	0.20494652940228864
46-47	0.21035072911948033
48-49	0.2080444782847552
50-51	0.2070806719657656
52-53	0.21110800551297215
54-55	0.21017862084823216
56-57	0.2203330088518726
58-59	0.21702853004390826
60-61	0.22398170420233326
62-63	0.219369202532883
64-65	0.21991994900087708
66-67	0.21017862084823216
68-69	0.2090082846037448
70-71	0.19200398740442828
72-73	0.19909484817985176
74-75	0.19971443795634508
76-77	0.19789009028111476
78-79	0.22480782390432433
80-81	0.23131351655750412
82-83	0.23210521460524558
84-85	0.22656332827105538
86-87	0.2431201439651267
88-89	0.2418465427578905
90-91	0.24143348290689495
92-93	0.2448412266776082
94-95	0.2556152044577419
96-97	0.254961193026999
98-99	0.24590829795934666
100-101	0.2544792898675042
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1452574.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.7426230589295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.27834402068805	57.24771811986014
2	9.775776486010438	13.440250361723239
3	2.9145656256572017	6.01064658555198
4	1.30992374562026	3.601903771244583
5	0.7390114338480521	2.5400792216627828
6	0.478199603725318	1.9723617063491394
7	0.3268106554468973	1.572607518931942
8	0.23021938882783136	1.2660707733638987
9	0.16645137650128317	1.0298073809220896
>10	0.7477559177394972	8.375818416948002
>50	0.02070963397439878	0.9519972819534662
>100	0.011329785614570593	1.4391696125898155
>500	6.015508974976894E-4	0.2330884917875949
>1k	3.007754487488447E-4	0.31848075711131757
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1844	0.12694706087263025	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1759	0.12109537965019337	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.884330849925718E-5
2	0.0	0.0	0.0	0.0	6.884330849925718E-5
3	0.0	0.0	0.0	0.0	6.884330849925718E-5
4	0.0	0.0	0.0	6.884330849925718E-5	6.884330849925718E-5
5	0.0	0.0	0.0	6.884330849925718E-5	6.884330849925718E-5
6	0.0	0.0	0.0	6.884330849925718E-5	6.884330849925718E-5
7	0.0	0.0	0.0	6.884330849925718E-5	6.884330849925718E-5
8	0.0	6.884330849925718E-5	0.0	6.884330849925718E-5	6.884330849925718E-5
9	0.0	6.884330849925718E-5	0.0	6.884330849925718E-5	6.884330849925718E-5
10-11	0.0	6.884330849925718E-5	0.0	1.0326496274888576E-4	6.884330849925718E-5
12-13	0.0	6.884330849925718E-5	0.0	1.3768661699851436E-4	2.753732339970287E-4
14-15	0.0	6.884330849925718E-5	0.0	1.3768661699851436E-4	3.786381967459145E-4
16-17	0.0	6.884330849925718E-5	0.0	1.3768661699851436E-4	4.130598509955431E-4
18-19	0.0	6.884330849925718E-5	0.0	1.3768661699851436E-4	5.507464679940574E-4
20-21	0.0	6.884330849925718E-5	0.0	1.7210827124814295E-4	5.507464679940574E-4
22-23	0.0	6.884330849925718E-5	0.0	6.884330849925718E-4	5.507464679940574E-4
24-25	0.0	6.884330849925718E-5	0.0	0.0011359145902377435	5.507464679940574E-4
26-27	0.0	6.884330849925718E-5	0.0	0.0027537323399702872	5.507464679940574E-4
28-29	0.0	6.884330849925718E-5	0.0	0.008433305291159005	5.507464679940574E-4
30-31	0.0	6.884330849925718E-5	0.0	0.026298143846716242	5.507464679940574E-4
32-33	0.0	2.0652992549777155E-4	0.0	0.0721133656529719	6.884330849925718E-4
34-35	0.0	2.0652992549777155E-4	0.0	0.1443644179229423	7.572763934918289E-4
36-37	0.0	2.4095157974740014E-4	0.0	0.23317228588698408	7.572763934918289E-4
38-39	0.0	2.753732339970287E-4	0.0	0.35147951154295753	7.572763934918289E-4
40-41	0.0	2.753732339970287E-4	0.0	0.4740206006716353	7.572763934918289E-4
42-43	0.0	2.753732339970287E-4	0.0	0.6121891208296445	7.572763934918289E-4
44-45	0.0	2.753732339970287E-4	0.0	0.8085646583237756	7.572763934918289E-4
46-47	0.0	2.753732339970287E-4	0.0	1.0234934674584566	7.916980477414576E-4
48-49	0.0	2.753732339970287E-4	0.0	1.2230357971435535	0.0010326496274888578
50-51	0.0	2.753732339970287E-4	0.0	1.4101863312987841	0.0010326496274888578
52-53	0.0	2.753732339970287E-4	0.0	1.619745362370523	0.0012736012072362577
54-55	0.0	2.753732339970287E-4	0.0	1.8863754961881458	0.0013080228614858864
56-57	0.0	2.753732339970287E-4	0.0	2.2061182425129457	0.0013768661699851436
58-59	0.0	2.753732339970287E-4	0.0	2.5124365436803906	0.0013768661699851436
60-61	0.0	2.753732339970287E-4	0.0	2.8059155678127246	0.0013768661699851436
62-63	0.0	2.753732339970287E-4	0.0	3.112371555597168	0.0014457094784844009
64-65	0.0	2.753732339970287E-4	0.0	3.490631114146336	0.0014457094784844009
66-67	0.0	2.753732339970287E-4	0.0	3.961691452552503	0.0014457094784844009
68-69	0.0	2.753732339970287E-4	0.0	4.442389854148567	0.0014457094784844009
70-71	0.0	2.753732339970287E-4	0.0	4.906428175087809	0.0014457094784844009
72-73	0.0	2.753732339970287E-4	0.0	5.363926381719623	0.0014457094784844009
74-75	0.0	2.753732339970287E-4	0.0	5.888168175941466	0.0014457094784844009
76-77	0.0	2.753732339970287E-4	0.0	6.5258637425700865	0.0014457094784844009
78-79	0.0	2.753732339970287E-4	0.0	7.217291511482376	0.0014457094784844009
80-81	0.0	2.753732339970287E-4	0.0	7.874194361182287	0.0015145527869836579
82-83	0.0	2.753732339970287E-4	0.0	8.531613535695943	0.0015833960954829151
84-85	0.0	2.753732339970287E-4	0.0	9.258943089990595	0.0015833960954829151
86-87	0.0	2.753732339970287E-4	0.0	10.076147583531029	0.0016178177497325438
88-89	0.0	2.753732339970287E-4	0.0	10.96846012664415	0.0016522394039821724
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1745	0.0	60.37018	1
GTATCAA	2960	0.0	37.99457	1
TCAACGC	3655	0.0	30.770947	4
ATCAACG	3795	0.0	29.512775	3
TATCAAC	3835	0.0	29.45245	2
CAACGCA	3810	0.0	29.393545	5
AACGCAG	3980	0.0	28.246569	6
TAACCCG	105	6.9536334E-5	27.11608	5
GTACATG	3740	0.0	25.249128	1
TACATGG	3850	0.0	23.913921	2
ACGCAGA	4690	0.0	23.266453	7
ACATGGG	4045	0.0	21.939812	3
GTGGTAT	805	0.0	21.810759	1
CGCAGAG	4895	0.0	21.80971	8
TGGTATC	845	0.0	20.218811	2
GTGTAAG	715	0.0	19.910406	1
GTCTACG	120	0.004791371	19.772139	1
GAGTACA	3080	0.0	19.720783	1
CAGAGTA	4795	0.0	19.69176	10-11
GCAGAGT	5175	0.0	19.529419	9
>>END_MODULE