FastQCFastQC Report
Mon 15 Aug 2016
SRR2921382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452574
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17110.11779090084222904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.11228343616228847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.056.3263171
GTATCAA28900.039.1385081
GTGGTAT7500.029.1488671
TCAACGC37350.028.5892964
TATCAAC37850.028.5877862
ATCAACG38350.027.9675623
CAACGCA38900.027.5031685
AACGCAG41450.025.9223166
GTACATG35600.023.4956681
TACATGG37300.021.3752882
TGGTATC8700.021.2743782
ACGCAGA50350.020.5848127
ACATGGG39200.019.976043
CGCAGAG51600.019.6254588
GTATTAG3305.9480953E-1018.72211
CATGGGG34100.018.6492774
ACGTACT1300.007524358618.2859089
AGAGTAC47200.018.28203410-11
AGTACTT33500.017.13631812-13
GAGTACT30900.017.0428212-13
Produced by FastQC (version 0.11.5)