##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921382_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1452574
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.476077638729592	33.0	31.0	34.0	30.0	34.0
2	31.606356027300503	34.0	31.0	34.0	29.0	34.0
3	31.67124222242722	34.0	31.0	34.0	28.0	34.0
4	35.17190242975573	37.0	35.0	37.0	33.0	37.0
5	34.91484220425259	37.0	35.0	37.0	32.0	37.0
6	34.87340817059923	37.0	35.0	37.0	32.0	37.0
7	34.74504431443768	37.0	35.0	37.0	32.0	37.0
8	34.71176339380988	37.0	35.0	37.0	32.0	37.0
9	36.33370554615462	39.0	37.0	39.0	32.0	39.0
10-11	36.19011217328686	39.0	37.0	39.0	32.0	39.0
12-13	36.137214696118754	39.0	37.0	39.0	32.0	39.0
14-15	37.374094538384966	40.0	38.0	41.0	32.0	41.0
16-17	37.34538928825657	40.0	38.0	41.0	32.0	41.0
18-19	37.3177356196655	40.0	38.0	41.0	31.5	41.0
20-21	37.286079401118286	40.0	38.0	41.0	31.0	41.0
22-23	37.178026386263284	40.0	38.0	41.0	31.0	41.0
24-25	37.10098418393831	40.0	37.5	41.0	31.0	41.0
26-27	36.95856252418122	40.0	37.0	41.0	30.5	41.0
28-29	36.79258991280307	40.0	37.0	41.0	30.0	41.0
30-31	36.63888621164911	40.0	37.0	41.0	30.0	41.0
32-33	36.555953431632396	40.0	36.5	41.0	30.0	41.0
34-35	36.6637617085257	40.0	37.0	41.0	30.0	41.0
36-37	36.61532871991375	40.0	37.0	41.0	30.0	41.0
38-39	36.50918094362146	40.0	37.0	41.0	30.0	41.0
40-41	36.37294278983377	40.0	36.5	41.0	29.5	41.0
42-43	36.24441646346416	40.0	36.0	41.0	29.0	41.0
44-45	36.06939336653417	40.0	36.0	41.0	28.0	41.0
46-47	35.865349028689764	40.0	35.5	41.0	27.5	41.0
48-49	35.669713900978536	40.0	35.0	41.0	27.0	41.0
50-51	35.502018485805195	39.5	35.0	41.0	26.0	41.0
52-53	35.317584508603346	39.0	35.0	41.0	26.0	41.0
54-55	35.10694842397014	39.0	35.0	41.0	26.0	41.0
56-57	34.83364978307473	39.0	35.0	41.0	24.5	41.0
58-59	34.600544963630085	38.5	34.5	40.5	24.0	41.0
60-61	34.32410087196934	38.0	34.0	40.0	23.5	41.0
62-63	34.018954283912564	37.0	34.0	40.0	23.0	41.0
64-65	33.69931824471593	37.0	34.0	40.0	22.0	41.0
66-67	33.3581865708735	36.0	34.0	39.0	22.0	41.0
68-69	32.98890521240226	36.0	33.0	39.0	21.0	41.0
70-71	32.60130637062208	35.0	33.0	38.5	20.0	40.0
72-73	32.202961776818256	35.0	33.0	37.5	18.0	40.0
74-75	31.799917594559727	35.0	32.5	37.0	15.5	39.0
76-77	29.044598416328533	33.0	28.5	34.5	7.5	36.5
78-79	30.688123290104325	34.5	31.5	36.0	10.0	37.5
80-81	30.879643997483086	35.0	32.0	36.0	7.5	37.0
82-83	30.71957401137567	35.0	32.0	35.0	6.5	37.0
84-85	30.480668798973408	35.0	32.0	35.0	2.0	36.5
86-87	30.225131731670814	35.0	32.0	35.0	2.0	36.0
88-89	29.93802312309046	35.0	31.0	35.0	2.0	36.0
90-91	29.74966335622144	34.5	31.0	35.0	2.0	35.0
92-93	29.60360745820867	34.0	31.0	35.0	2.0	35.0
94-95	29.499718086651697	34.0	31.0	35.0	2.0	35.0
96-97	29.296823087842682	34.0	31.0	35.0	2.0	35.0
98-99	29.014921098684127	34.0	31.0	35.0	2.0	35.0
100-101	27.92030836294743	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24530.0
3	8474.0
4	4636.0
5	3516.0
6	3044.0
7	3125.0
8	3389.0
9	3568.0
10	4100.0
11	4434.0
12	4974.0
13	5476.0
14	6123.0
15	6881.0
16	8007.0
17	8995.0
18	8192.0
19	6802.0
20	6354.0
21	6057.0
22	6131.0
23	6900.0
24	7951.0
25	9281.0
26	11096.0
27	13398.0
28	15795.0
29	19232.0
30	23549.0
31	28685.0
32	36232.0
33	47246.0
34	68042.0
35	101364.0
36	175033.0
37	336799.0
38	362749.0
39	52412.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.873061569831066	19.92667253709298	13.928839124345696	25.271426768730258
2	15.904616087877226	21.11303260698953	37.03630525715765	25.946046047975592
3	18.32685588392227	24.050672128608067	29.838998950067985	27.78347303740168
4	13.829306779096248	17.12033673798161	34.64576144697093	34.40459503595121
5	14.437396105479019	36.93356397285762	34.05564270338536	14.573397218277998
6	33.587298571282034	34.90366325407332	16.617753058219243	14.891285116425404
7	28.251680467474245	32.27096488160311	20.990629035944515	18.486725614978123
8	26.380032742689004	34.629662012284705	19.9191346457029	19.07117059932339
9	26.354457673068637	15.733793940962732	19.930000123917953	37.98174826205068
10-11	25.390252945935927	25.516271049537487	27.825155653205897	21.26832035132069
12-13	25.829588540331034	23.316185594867036	27.576592562505486	23.277633302296444
14-15	23.320242052718307	25.150887077980038	24.885928921540785	26.642941947760868
16-17	23.119958088193783	27.95010787746442	26.13649975835999	22.79343427598181
18-19	23.107399532484287	27.01412632700008	27.750510043739673	22.12796409677596
20-21	23.450992811005342	26.13854433614961	27.839497992348033	22.570964860497018
22-23	23.491452753149066	25.987609572806324	27.383408613543004	23.137529060501606
24-25	23.307180556736427	26.27808457445142	27.353500333378193	23.061234535433954
26-27	23.063953766553315	26.408333255882877	27.548817021751187	22.97889595581262
28-29	23.062976166379574	26.735300718905382	27.38191624566433	22.81980686905072
30-31	23.571831610446043	26.206132989891955	27.187502259256235	23.03453314040576
32-33	23.274776733382428	26.293218154921437	27.005956874883598	23.426048236812544
34-35	23.63399138321293	26.428956260252445	27.038328005935014	22.89872435059961
36-37	23.52483365632519	26.37417258435939	27.229074086185474	22.871919673129945
38-39	23.431437477798003	26.32007225803609	27.19452116659277	23.05396909757313
40-41	23.549253671745	26.231987227635457	27.173150495934284	23.045608604685267
42-43	23.5008192996709	26.27277171144128	26.791202511601007	23.435206477286812
44-45	23.32682484855036	26.357160216817917	26.909635563866342	23.406379370765382
46-47	23.52709923966323	26.350503993773273	26.62965330612411	23.49274346043939
48-49	23.2358015885256	26.524046829558472	26.729669736494227	23.510481845421697
50-51	23.281647591456363	26.493635782817993	26.77181210826054	23.452904517465107
52-53	23.606490902932485	26.39357794122912	26.537599927300565	23.462331228537835
54-55	23.054178103363647	26.335107441270967	26.777739454659738	23.832975000705652
56-57	23.17982880397553	26.463197260973764	26.547087856896486	23.80988607815423
58-59	23.15605719020566	26.38611340895032	26.658101836791108	23.799727564052915
60-61	23.173427288390275	26.273565501465608	26.891754892966624	23.661252317177492
62-63	23.13069858586248	26.224546144344206	26.86847515791822	23.776280111875092
64-65	22.91772546783517	26.138994596842945	26.762396065322697	24.180883869999185
66-67	22.934336131982665	26.283314951522634	26.60485603478346	24.177492881711242
68-69	22.9453147836937	26.183479472514847	26.66622595951072	24.204979784280734
70-71	22.846823964192417	26.059430447877148	26.633231931778816	24.460513656151623
72-73	22.871477481497397	26.11807512319002	26.550341957323504	24.46010543798908
74-75	22.767097073844045	26.323181012367534	26.439519373487453	24.470202540300964
76-77	22.804744900846536	25.945467319454014	26.57719366776032	24.672594111939134
78-79	22.647744804944516	26.047390080317673	26.584919484261114	24.719945630476694
80-81	22.717285290779046	26.087080803182754	26.49653125998744	24.69910264605076
82-83	22.589516410839916	26.039588450872486	26.574423292427447	24.79647184586015
84-85	22.548775205372003	26.06463235501725	26.52850554464814	24.85808689496261
86-87	22.52455600754043	26.19414266186019	26.340968846115686	24.94033248448369
88-89	22.375987970240356	25.9232761146665	26.418637430236817	25.28209848485633
90-91	22.299302992463588	25.69645501359206	26.516053550511636	25.488188443432712
92-93	22.2411186306418	25.62828261955193	26.64359146561967	25.487007284186596
94-95	22.194063806694196	25.50629209777786	26.656002269196517	25.643641826331425
96-97	22.046656694786453	25.399790349537927	26.685405539385197	25.868147416290427
98-99	21.919022902192804	25.38658831231909	26.57581013757563	26.11857864791248
100-101	21.750178391881096	25.370784900181437	26.593832064137775	26.28520464379969
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	127.0
1	100.5
2	98.5
3	118.5
4	116.0
5	140.5
6	207.0
7	385.5
8	557.0
9	618.0
10	570.0
11	467.5
12	462.5
13	471.5
14	511.0
15	567.5
16	642.0
17	733.5
18	820.5
19	972.5
20	1075.0
21	1208.0
22	1423.5
23	1693.0
24	2074.5
25	2629.5
26	3433.5
27	4600.0
28	6134.5
29	8183.0
30	10749.0
31	13612.5
32	17225.0
33	19719.5
34	21346.0
35	23799.5
36	27232.0
37	30779.0
38	33890.5
39	36993.0
40	39561.0
41	42071.0
42	44948.5
43	48430.5
44	51600.0
45	54845.0
46	56969.5
47	58603.5
48	61227.0
49	62490.0
50	62208.0
51	61374.0
52	60682.0
53	60115.0
54	58667.0
55	55305.5
56	50326.5
57	44724.0
58	39883.0
59	34937.5
60	28872.5
61	23588.5
62	19318.5
63	14638.5
64	10498.5
65	7697.0
66	5715.5
67	4421.0
68	3375.0
69	2568.0
70	1911.5
71	1349.5
72	945.0
73	699.0
74	529.0
75	393.5
76	279.5
77	152.0
78	80.0
79	49.5
80	30.5
81	20.5
82	12.0
83	11.0
84	7.0
85	4.5
86	3.5
87	2.0
88	2.5
89	1.5
90	0.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16295211121774175
2	1.3768661699851436E-4
3	0.006815487541426461
4	0.003442165424962859
5	0.02616045722971773
6	8.949630104903433E-4
7	1.3768661699851436E-4
8	0.0024783591059732582
9	0.0
10-11	0.10959854713081744
12-13	1.0326496274888576E-4
14-15	0.005541886334190203
16-17	0.0
18-19	1.7210827124814295E-4
20-21	4.4748150524517166E-4
22-23	6.884330849925718E-5
24-25	0.001342444515735515
26-27	0.0022374075262258583
28-29	0.006058211147934632
30-31	0.014835732981589923
32-33	0.015042262907087696
34-35	0.012150843950118891
36-37	6.195897764933146E-4
38-39	1.3768661699851436E-4
40-41	0.004337128435453202
42-43	0.007848137168915317
44-45	0.007641607243417547
46-47	0.008639835216656776
48-49	0.010877242742882634
50-51	0.00536977806294206
52-53	0.0012391795529866291
54-55	8.949630104903433E-4
56-57	0.005989367839435374
58-59	0.008433305291159005
60-61	0.007331812355170889
62-63	0.016522394039821725
64-65	0.0316679219096583
66-67	0.034731449137875244
68-69	0.02495569933098073
70-71	0.040686395323060995
72-73	0.023819784740742984
74-75	0.023716519777994098
76-77	0.2483866570653199
78-79	0.07059881286598824
80-81	0.05163248137444289
82-83	0.017658308630059467
84-85	0.031186018750163504
86-87	0.0824742835821101
88-89	0.2639108231319024
90-91	0.32149825069153104
92-93	0.05500580349090649
94-95	0.005335356408692432
96-97	0.01077397778013375
98-99	0.0018931909837295725
100-101	9.29384664739972E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1452574.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.90100164975242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.69593641974188	56.15136915264715
2	10.141379738698062	13.772196847362055
3	2.9829459322598497	6.076350500024948
4	1.3587698659225866	3.6904733963057366
5	0.7718063865636263	2.620321336367312
6	0.47749523953989675	1.9453443028648474
7	0.32767098449180343	1.5574431640987756
8	0.23535484244963006	1.2784663636359639
9	0.18766460343068816	1.146835309243256
>10	0.7860396204861262	8.746713807718976
>50	0.02263317651917865	1.0389180776152993
>100	0.011388118073909171	1.4173946736002658
>500	6.100478817847613E-4	0.2508154307874825
>1k	3.0502394089238063E-4	0.3073576377280132
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1711	0.11779090084222904	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1631	0.11228343616228847	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.884330849925718E-5	0.0
5	0.0	0.0	0.0	6.884330849925718E-5	0.0
6	0.0	0.0	0.0	6.884330849925718E-5	0.0
7	0.0	0.0	0.0	6.884330849925718E-5	0.0
8	0.0	0.0	0.0	6.884330849925718E-5	0.0
9	0.0	0.0	0.0	1.3768661699851436E-4	0.0
10-11	0.0	0.0	0.0	1.3768661699851436E-4	0.0
12-13	0.0	0.0	0.0	1.3768661699851436E-4	1.7210827124814295E-4
14-15	0.0	3.442165424962859E-5	0.0	1.3768661699851436E-4	2.0652992549777155E-4
16-17	0.0	6.884330849925718E-5	0.0	1.3768661699851436E-4	2.0652992549777155E-4
18-19	6.884330849925718E-5	6.884330849925718E-5	0.0	1.3768661699851436E-4	4.130598509955431E-4
20-21	6.884330849925718E-5	6.884330849925718E-5	0.0	1.7210827124814295E-4	4.130598509955431E-4
22-23	6.884330849925718E-5	6.884330849925718E-5	0.0	6.195897764933146E-4	4.4748150524517166E-4
24-25	6.884330849925718E-5	6.884330849925718E-5	0.0	0.0011359145902377435	4.819031594948003E-4
26-27	6.884330849925718E-5	6.884330849925718E-5	0.0	0.0026160457229717727	5.163248137444289E-4
28-29	6.884330849925718E-5	6.884330849925718E-5	0.0	0.008502148599658262	5.507464679940574E-4
30-31	6.884330849925718E-5	6.884330849925718E-5	0.0	0.026745625351961413	5.507464679940574E-4
32-33	6.884330849925718E-5	6.884330849925718E-5	0.0	0.0740409782909511	5.507464679940574E-4
34-35	6.884330849925718E-5	6.884330849925718E-5	0.0	0.1478410050021548	5.507464679940574E-4
36-37	6.884330849925718E-5	6.884330849925718E-5	0.0	0.23840437733292763	5.507464679940574E-4
38-39	6.884330849925718E-5	6.884330849925718E-5	0.0	0.35815731246738547	5.85168122243686E-4
40-41	6.884330849925718E-5	6.884330849925718E-5	0.0	0.48328002566478545	6.195897764933147E-4
42-43	6.884330849925718E-5	6.884330849925718E-5	0.0	0.6245464947052611	6.195897764933147E-4
44-45	6.884330849925718E-5	6.884330849925718E-5	0.0	0.8252935822890951	6.195897764933147E-4
46-47	6.884330849925718E-5	6.884330849925718E-5	0.0	1.0428384371467478	6.195897764933147E-4
48-49	6.884330849925718E-5	6.884330849925718E-5	0.0	1.2455131373685608	7.228547392422004E-4
50-51	6.884330849925718E-5	6.884330849925718E-5	0.0	1.4343503325820233	7.572763934918289E-4
52-53	6.884330849925718E-5	6.884330849925718E-5	0.0	1.6456304463662437	8.261197019910861E-4
54-55	6.884330849925718E-5	6.884330849925718E-5	0.0	1.915289685757834	8.949630104903433E-4
56-57	6.884330849925718E-5	6.884330849925718E-5	0.0	2.237889429385353	8.949630104903433E-4
58-59	6.884330849925718E-5	6.884330849925718E-5	0.0	2.548372750717003	8.949630104903433E-4
60-61	6.884330849925718E-5	6.884330849925718E-5	0.0	2.8449841453860527	8.949630104903433E-4
62-63	6.884330849925718E-5	6.884330849925718E-5	0.0	3.1535742757339733	0.0010326496274888578
64-65	6.884330849925718E-5	6.884330849925718E-5	0.0	3.5350006264741074	0.0010326496274888578
66-67	6.884330849925718E-5	6.884330849925718E-5	0.0	4.010191563390229	0.0010326496274888578
68-69	6.884330849925718E-5	6.884330849925718E-5	0.0	4.494779611916501	0.0010326496274888578
70-71	6.884330849925718E-5	6.884330849925718E-5	0.0	4.962707579785953	0.0010326496274888578
72-73	6.884330849925718E-5	6.884330849925718E-5	0.0	5.426918008996443	0.0011014929359881148
74-75	6.884330849925718E-5	6.884330849925718E-5	0.0	5.960178276631689	0.0011014929359881148
76-77	6.884330849925718E-5	6.884330849925718E-5	0.0	6.6079938096097	0.0011014929359881148
78-79	6.884330849925718E-5	6.884330849925718E-5	0.0	7.309713653142628	0.0011014929359881148
80-81	6.884330849925718E-5	6.884330849925718E-5	0.0	7.980970332664635	0.0011359145902377435
82-83	6.884330849925718E-5	6.884330849925718E-5	0.0	8.649955183006167	0.0012391795529866291
84-85	1.3768661699851436E-4	6.884330849925718E-5	0.0	9.388127558389453	0.0013080228614858864
86-87	1.3768661699851436E-4	6.884330849925718E-5	0.0	10.223024782214193	0.0013768661699851436
88-89	1.3768661699851436E-4	6.884330849925718E-5	0.0	11.134923246595354	0.0013768661699851436
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1620	0.0	56.326317	1
GTATCAA	2890	0.0	39.138508	1
GTGGTAT	750	0.0	29.148867	1
TCAACGC	3735	0.0	28.589296	4
TATCAAC	3785	0.0	28.587786	2
ATCAACG	3835	0.0	27.967562	3
CAACGCA	3890	0.0	27.503168	5
AACGCAG	4145	0.0	25.922316	6
GTACATG	3560	0.0	23.495668	1
TACATGG	3730	0.0	21.375288	2
TGGTATC	870	0.0	21.274378	2
ACGCAGA	5035	0.0	20.584812	7
ACATGGG	3920	0.0	19.97604	3
CGCAGAG	5160	0.0	19.625458	8
GTATTAG	330	5.9480953E-10	18.7221	1
CATGGGG	3410	0.0	18.649277	4
ACGTACT	130	0.0075243586	18.285908	9
AGAGTAC	4720	0.0	18.282034	10-11
AGTACTT	3350	0.0	17.136318	12-13
GAGTACT	3090	0.0	17.04282	12-13
>>END_MODULE