FastQCFastQC Report
Mon 15 Aug 2016
SRR2921381_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921381_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431039
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.047.2756351
GTATCAA47700.041.0893971
TCAACGC54300.035.1342474
CAACGCA54900.034.4004825
ATCAACG56800.033.4202583
TATCAAC58700.032.8245472
AACGCAG60700.031.5719076
ACGCAGA68550.027.2004157
GTACATG83450.026.557591
CGCAGAG70200.026.1578678
TACATGG86750.025.1651272
ACATGGG92000.023.0062143
CATGGGG79250.021.6178444
GCAGAGT82900.020.490419
GTATATA12000.018.9827041
GAGTACA64900.018.9388311
AGAGTAC65800.018.49651510-11
GTGTAGC18850.018.378481
TAGCCCT21950.017.5127744
CAGAGTA74950.017.0930910-11
Produced by FastQC (version 0.11.5)