##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921381_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3431039
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.85992435527547	31.0	31.0	34.0	25.0	34.0
2	29.923541527799596	31.0	31.0	34.0	25.0	34.0
3	29.96412865024268	31.0	31.0	34.0	25.0	34.0
4	33.40801284975193	37.0	35.0	37.0	28.0	37.0
5	33.392132820408044	37.0	35.0	37.0	27.0	37.0
6	33.428737767189475	37.0	35.0	37.0	28.0	37.0
7	33.33260537114268	37.0	35.0	37.0	27.0	37.0
8	33.29609981116507	37.0	35.0	37.0	27.0	37.0
9	34.791379520897316	39.0	35.0	39.0	27.0	39.0
10-11	34.673148133845174	39.0	35.0	39.0	27.0	39.0
12-13	34.55025052178072	39.0	35.0	39.0	27.0	39.0
14-15	35.62162379967118	40.0	36.0	41.0	26.5	41.0
16-17	35.5606782668457	40.0	36.0	41.0	26.0	41.0
18-19	35.50832182321449	40.0	36.0	41.0	25.5	41.0
20-21	35.4442509980213	40.0	36.0	41.0	25.0	41.0
22-23	35.31972355895692	40.0	36.0	41.0	25.0	41.0
24-25	35.21389890933912	40.0	35.5	41.0	24.0	41.0
26-27	35.061280853992045	39.0	35.0	41.0	23.5	41.0
28-29	34.9191511667457	39.0	35.0	41.0	23.0	41.0
30-31	34.77185409434286	39.0	35.0	41.0	22.0	41.0
32-33	34.59404702190794	39.0	34.5	41.0	20.5	41.0
34-35	34.4269789705101	39.0	34.0	41.0	19.0	41.0
36-37	34.25448909207969	39.0	34.0	40.5	18.0	41.0
38-39	34.48965998346274	39.0	34.5	41.0	19.0	41.0
40-41	34.62181761851148	39.0	35.0	41.0	19.0	41.0
42-43	34.46495041297986	39.0	35.0	41.0	16.0	41.0
44-45	34.351752195180524	39.0	34.5	41.0	15.0	41.0
46-47	34.20611496983858	39.0	34.0	41.0	14.0	41.0
48-49	34.06938874783994	39.0	34.0	41.0	10.5	41.0
50-51	32.392768779369746	37.0	32.0	39.5	8.5	40.0
52-53	32.54598461282428	37.5	32.0	39.5	8.0	40.0
54-55	33.39951760968033	38.0	33.0	40.0	7.0	41.0
56-57	33.4244902200179	38.0	33.0	41.0	4.0	41.0
58-59	33.257963841273735	38.0	33.0	40.0	2.0	41.0
60-61	33.018249719691326	37.5	33.0	40.0	2.0	41.0
62-63	32.74071061273276	37.0	33.0	40.0	2.0	41.0
64-65	32.46136010112389	37.0	32.0	40.0	2.0	41.0
66-67	32.12625679859658	36.0	32.0	39.0	2.0	41.0
68-69	31.762213137186723	36.0	32.0	39.0	2.0	41.0
70-71	31.379348792013147	35.0	31.5	39.0	2.0	40.5
72-73	31.025516177461114	35.0	31.0	37.5	2.0	40.0
74-75	30.6246912378437	35.0	31.0	37.0	2.0	39.0
76-77	30.244100547968124	35.0	31.0	36.5	2.0	39.0
78-79	29.895510951638848	35.0	30.5	36.0	2.0	38.5
80-81	29.538981049180727	35.0	30.0	36.0	2.0	37.0
82-83	29.23526138875134	35.0	30.0	35.0	2.0	37.0
84-85	28.996063437343615	34.5	30.0	35.0	2.0	36.5
86-87	28.76420393356065	34.0	30.0	35.0	2.0	36.0
88-89	28.556512764792238	34.0	29.0	35.0	2.0	36.0
90-91	28.300694046322413	34.0	29.0	35.0	2.0	35.5
92-93	28.107912501140326	34.0	29.0	35.0	2.0	35.0
94-95	27.89338827101645	34.0	29.0	35.0	2.0	35.0
96-97	27.632799568876948	34.0	29.0	35.0	2.0	35.0
98-99	27.392072634557636	34.0	29.0	35.0	2.0	35.0
100-101	26.34779275315728	33.0	25.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	153953.0
3	24625.0
4	16496.0
5	12280.0
6	10997.0
7	11248.0
8	11792.0
9	12642.0
10	13715.0
11	14503.0
12	14575.0
13	14239.0
14	15025.0
15	15821.0
16	16308.0
17	16825.0
18	15964.0
19	14968.0
20	15561.0
21	16346.0
22	18014.0
23	20596.0
24	23806.0
25	28384.0
26	33337.0
27	39789.0
28	46721.0
29	55075.0
30	64674.0
31	78760.0
32	97965.0
33	124790.0
34	171805.0
35	245719.0
36	397250.0
37	693979.0
38	726138.0
39	126342.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.257697926903596	18.52844857501961	13.458854474989575	24.75499902308722
2	15.775499556270786	19.631962852150824	39.560056263056325	25.03248132852206
3	18.894653717575753	23.727525527641237	29.629910440379515	27.74791031440349
4	13.369694010957781	15.997094178762833	36.773453555708564	33.85975825457082
5	14.393909349433228	37.05386824430722	34.44484751211341	14.107374894146135
6	33.34443995515374	35.31873672525482	17.2963625667782	14.04046075281324
7	28.795353201626238	31.770146022828804	21.157583564568455	18.276917210976503
8	26.439348334030555	34.858881987939775	20.053727314202753	18.64804236382691
9	26.675944796487993	15.67658001993418	19.600855787952558	38.046619395625264
10-11	25.505902560954063	25.319747560350603	28.066433910966282	21.107915967729053
12-13	25.921612130629928	22.769202287466893	27.809646952838126	23.499538629065057
14-15	23.310261310559323	24.431986180697184	24.914868639058778	27.34288386968472
16-17	23.573054601369016	27.431241716264605	25.77154995722925	23.224153725137132
18-19	23.58514699702363	26.961433752110125	26.892764056552576	22.560655194313664
20-21	24.065190971956493	26.11739406955389	26.816364389335362	23.00105056915426
22-23	23.989478848709606	25.888301707427136	26.482784664138926	23.63943477972433
24-25	23.77641086351787	26.06932286715693	26.482915677686865	23.671350591638333
26-27	23.64984027273027	26.280566044172016	26.460191801006967	23.60940188209075
28-29	23.752378081594365	26.473550601903167	26.30436645807376	23.469704858428713
30-31	24.090918895851164	26.101338793290406	26.235005328588517	23.57273698226991
32-33	23.816128098555648	26.148966193493926	26.15459191510932	23.88031379284111
34-35	24.111007147271096	26.247391187752868	26.19601653316544	23.445585131810603
36-37	24.144022071776412	26.094582668315947	26.220858248411243	23.540537011496397
38-39	23.925433511606144	26.237976349765457	26.225078447663886	23.61151169096452
40-41	24.098211959173465	26.03788911733519	26.246682363200858	23.617216560290487
42-43	23.896691410708343	26.04107115574666	26.13349980617443	23.928737627370566
44-45	23.879525602123753	26.059407791176152	26.232838706474343	23.82822790022575
46-47	24.051498014398202	26.0129022497451	26.020408312277905	23.91519142357879
48-49	23.765459907373806	26.12511326374078	26.141161850508652	23.96826497837676
50-51	23.86789799567822	26.14584303796877	26.223251347599668	23.763007618753342
52-53	24.075900587847528	26.000387867799002	26.16079223166222	23.76291931269125
54-55	23.65952250602632	25.857809653999336	26.464932124218937	24.01773571575541
56-57	23.690945265697287	26.04033352129725	26.270855852125706	23.997865360879757
58-59	23.79827646738905	25.96655164376432	26.3487589034678	23.88641298537883
60-61	23.887630508210698	25.947924801332206	26.418832937076083	23.745611753381016
62-63	23.847973025766866	25.96987028282081	26.410434624624312	23.771722066788012
64-65	23.637093080588212	25.779240012057734	26.429767771725544	24.15389913562851
66-67	23.639549536222432	25.91146599624083	26.292070708390902	24.156913759145837
68-69	23.680461761169788	25.840885682830674	26.40183391358684	24.076818642412697
70-71	23.622987426314772	25.6828157299112	26.389744236659425	24.304452607114605
72-73	23.625626374563446	25.868324013095933	26.25201174323629	24.254037869104327
74-75	23.569230400820494	25.902015491741903	26.3400058873759	24.1887482200617
76-77	23.631585272066573	25.64003274794986	26.338284803494048	24.39009717648952
78-79	23.508944579884293	25.65954628917215	26.443661210709546	24.387847920234012
80-81	23.659411880442168	25.716964158036443	26.350308850480843	24.273315111040546
82-83	23.582875804640203	25.787908739549042	26.432468063793685	24.19674739201707
84-85	23.588304902596843	25.76307586518348	26.40074091189651	24.247878320323167
86-87	23.47613454042452	25.84194405704121	26.363736895102534	24.318184507431738
88-89	23.381302110388567	25.629298038846766	26.491798192230764	24.497601658533906
90-91	23.423433030433262	25.50660993729989	26.58276072725233	24.487196305014518
92-93	23.493457653666507	25.44242225389671	26.68823563870652	24.375884453730258
94-95	23.44245740123111	25.356969413975822	26.685951070075465	24.514622114717607
96-97	23.33440085183301	25.207735106582856	26.777498658053272	24.68036538353086
98-99	23.20980346383056	25.150418085289456	26.737167094164803	24.90261135671518
100-101	23.376288294542963	25.07923386734469	26.8662455762643	24.678232261848045
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	35.5
2	42.5
3	64.5
4	71.5
5	87.5
6	147.0
7	264.0
8	413.5
9	479.0
10	434.0
11	443.0
12	506.5
13	570.5
14	659.0
15	746.0
16	854.5
17	980.0
18	1100.0
19	1205.5
20	1300.0
21	1427.0
22	1576.0
23	1772.0
24	2091.5
25	2626.5
26	3489.5
27	4902.0
28	7168.0
29	10578.0
30	15583.0
31	22085.0
32	30289.0
33	37884.0
34	44582.0
35	52431.5
36	62285.0
37	72689.0
38	82112.0
39	91300.5
40	99199.5
41	106780.0
42	114820.0
43	122536.5
44	129872.0
45	137350.5
46	142721.5
47	146859.0
48	151472.5
49	153380.5
50	151706.5
51	148822.0
52	146105.0
53	142210.0
54	137409.5
55	130232.0
56	119835.0
57	107767.0
58	95831.0
59	82912.0
60	68260.5
61	55801.0
62	45467.0
63	34979.5
64	25582.5
65	18949.5
66	14305.0
67	10828.5
68	8153.5
69	6158.0
70	4698.0
71	3433.0
72	2401.0
73	1751.0
74	1314.5
75	1006.0
76	751.0
77	485.0
78	319.5
79	238.5
80	189.0
81	138.5
82	105.5
83	90.0
84	71.5
85	63.5
86	56.5
87	50.0
88	45.0
89	41.5
90	34.0
91	31.0
92	27.5
93	21.0
94	13.5
95	16.5
96	18.0
97	11.5
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05447329511556121
2	0.06254665132048921
3	0.0528994278409543
4	0.06004012195722637
5	0.051966765752298354
6	0.01903213574663535
7	0.015971838268233035
8	0.02197585046395567
9	0.021859267702873678
10-11	0.024278359995325034
12-13	0.030428100642400157
14-15	0.04759491221172362
16-17	0.032788901554310516
18-19	0.02478840957505875
20-21	0.028956243283739996
22-23	0.051194404960130155
24-25	0.046021044937116715
26-27	0.03293463000566301
28-29	0.021946704773685172
30-31	0.014193951161732641
32-33	0.010725614019543351
34-35	0.010463302807108867
36-37	0.0014864302037954102
38-39	0.007242704032218813
40-41	0.0038763768059762657
42-43	0.22902683414557515
44-45	0.20136757407887232
46-47	0.20810022853135743
48-49	0.20581229184512329
50-51	0.20367008361024166
52-53	0.20961580442542335
54-55	0.20694897376567273
56-57	0.2188695610863065
58-59	0.2148911743643835
60-61	0.21997709731658546
62-63	0.2178786076171096
64-65	0.21891327962171225
66-67	0.20826052982784513
68-69	0.20706555652675473
70-71	0.18962186089986152
72-73	0.19731632313127306
74-75	0.19711230329937957
76-77	0.19534898903801443
78-79	0.22223588831254906
80-81	0.23148964497343225
82-83	0.22872080439773493
84-85	0.2241303581801314
86-87	0.2425067158956806
88-89	0.24065596456350394
90-91	0.24010219644836445
92-93	0.24410972886055796
94-95	0.25522880969875306
96-97	0.2556659950528105
98-99	0.24517354655543117
100-101	0.25454388597739636
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3431039.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.37384667059126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.62711974710466	49.66649757926716
2	11.47376418957174	14.31325616589737
3	3.709813513358326	6.94186017776099
4	1.6656472712224208	4.155713100100641
5	0.9140636405077526	2.8506832680096506
6	0.5817785457063188	2.17726594836753
7	0.39734413175651695	1.734871736474814
8	0.27730063149086526	1.3837045656215488
9	0.2071972050908198	1.163131803081886
>10	1.0846992923533727	11.761620188871696
>50	0.04748752553371753	1.9895039585821919
>100	0.012611863133104253	1.2904553264605567
>500	0.0010317499913310834	0.47508037040703666
>1k	1.4069317910596184E-4	0.09635581109706319
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.9145690270498237E-5
6	0.0	0.0	0.0	0.0	5.8291380540996475E-5
7	0.0	0.0	0.0	2.9145690270498237E-5	5.8291380540996475E-5
8	0.0	0.0	0.0	2.9145690270498237E-5	5.8291380540996475E-5
9	0.0	0.0	0.0	2.9145690270498237E-5	5.8291380540996475E-5
10-11	0.0	0.0	0.0	5.8291380540996475E-5	5.8291380540996475E-5
12-13	0.0	1.4572845135249119E-5	0.0	5.8291380540996475E-5	1.0200991594674383E-4
14-15	0.0	2.9145690270498237E-5	0.0	5.8291380540996475E-5	1.8944698675823853E-4
16-17	0.0	2.9145690270498237E-5	0.0	1.0200991594674383E-4	2.623112124344841E-4
18-19	0.0	2.9145690270498237E-5	0.0	1.457284513524912E-4	2.623112124344841E-4
20-21	0.0	2.9145690270498237E-5	0.0	1.603012964877403E-4	3.4974828324597885E-4
22-23	0.0	2.9145690270498237E-5	0.0	2.7688405756973324E-4	3.64321128381228E-4
24-25	0.0	2.9145690270498237E-5	0.0	4.8090388946322093E-4	4.226125089222244E-4
26-27	0.0	4.3718535405747356E-5	0.0	6.849237213567086E-4	4.663310443279718E-4
28-29	0.0	5.8291380540996475E-5	0.0	0.0019964797835291294	4.663310443279718E-4
30-31	0.0	5.8291380540996475E-5	0.0	0.00973466055034641	4.8090388946322093E-4
32-33	0.0	5.8291380540996475E-5	0.0	0.028154736801301296	5.246224248689682E-4
34-35	1.4572845135249119E-5	5.8291380540996475E-5	0.0	0.0512672691858064	5.537681151394665E-4
36-37	2.9145690270498237E-5	5.8291380540996475E-5	0.0	0.08937525921448283	5.537681151394665E-4
38-39	2.9145690270498237E-5	5.8291380540996475E-5	0.0	0.15202392045091878	5.974866505452139E-4
40-41	2.9145690270498237E-5	5.8291380540996475E-5	0.0	0.22367859998093872	6.12059495680463E-4
42-43	2.9145690270498237E-5	5.8291380540996475E-5	0.0	0.29922422916207014	6.12059495680463E-4
44-45	2.9145690270498237E-5	5.8291380540996475E-5	0.0	0.39638138767877606	6.412051859509612E-4
46-47	2.9145690270498237E-5	5.8291380540996475E-5	0.0	0.5062897856888249	6.994965664919577E-4
48-49	2.9145690270498237E-5	5.8291380540996475E-5	0.0	0.620948931212965	7.28642256762456E-4
50-51	2.9145690270498237E-5	5.8291380540996475E-5	0.0	0.7403151057157905	7.28642256762456E-4
52-53	2.9145690270498237E-5	7.28642256762456E-5	0.0	0.8669968484765112	8.306521727091998E-4
54-55	2.9145690270498237E-5	8.743707081149471E-5	0.0	1.0153338391082118	8.452250178444489E-4
56-57	2.9145690270498237E-5	8.743707081149471E-5	0.0	1.1856612530490036	8.452250178444489E-4
58-59	2.9145690270498237E-5	1.1658276108199295E-4	0.0	1.3636831292212068	8.452250178444489E-4
60-61	2.9145690270498237E-5	1.1658276108199295E-4	0.0	1.553523582798097	8.597978629796979E-4
62-63	2.9145690270498237E-5	1.1658276108199295E-4	0.0	1.7504318662655831	9.035163983854453E-4
64-65	2.9145690270498237E-5	1.1658276108199295E-4	0.0	1.9684124837986392	9.035163983854453E-4
66-67	2.9145690270498237E-5	1.457284513524912E-4	0.0	2.22349556504604	9.035163983854453E-4
68-69	2.9145690270498237E-5	1.7487414162298942E-4	0.0	2.503294191642823	9.326620886559436E-4
70-71	2.9145690270498237E-5	2.1859267702873677E-4	0.0	2.790466677878042	9.472349337911927E-4
72-73	5.8291380540996475E-5	2.623112124344841E-4	0.0	3.0930572342663547	0.001122109075414182
74-75	5.8291380540996475E-5	2.623112124344841E-4	0.0	3.422520117084067	0.0011658276108199295
76-77	5.8291380540996475E-5	2.7688405756973324E-4	0.0	3.7942296779488665	0.0011658276108199295
78-79	5.8291380540996475E-5	2.914569027049824E-4	0.0	4.208856267736974	0.0011949733010904278
80-81	5.8291380540996475E-5	2.914569027049824E-4	0.0	4.636408971160048	0.001224118991360926
82-83	5.8291380540996475E-5	2.914569027049824E-4	0.0	5.073317441159952	0.0012969832170371715
84-85	5.8291380540996475E-5	2.914569027049824E-4	0.0	5.542781647192003	0.0013115560621724206
86-87	5.8291380540996475E-5	2.914569027049824E-4	0.0	6.056168408461693	0.001355274597578168
88-89	5.8291380540996475E-5	2.914569027049824E-4	0.0	6.611772702088201	0.0013989931329839154
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2620	0.0	47.275635	1
GTATCAA	4770	0.0	41.089397	1
TCAACGC	5430	0.0	35.134247	4
CAACGCA	5490	0.0	34.400482	5
ATCAACG	5680	0.0	33.420258	3
TATCAAC	5870	0.0	32.824547	2
AACGCAG	6070	0.0	31.571907	6
ACGCAGA	6855	0.0	27.200415	7
GTACATG	8345	0.0	26.55759	1
CGCAGAG	7020	0.0	26.157867	8
TACATGG	8675	0.0	25.165127	2
ACATGGG	9200	0.0	23.006214	3
CATGGGG	7925	0.0	21.617844	4
GCAGAGT	8290	0.0	20.49041	9
GTATATA	1200	0.0	18.982704	1
GAGTACA	6490	0.0	18.938831	1
AGAGTAC	6580	0.0	18.496515	10-11
GTGTAGC	1885	0.0	18.37848	1
TAGCCCT	2195	0.0	17.512774	4
CAGAGTA	7495	0.0	17.09309	10-11
>>END_MODULE