FastQCFastQC Report
Mon 15 Aug 2016
SRR2921381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431039
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.048.1789971
GTATCAA44650.040.1341741
TCAACGC55150.031.668334
CAACGCA56800.030.7226095
ATCAACG57900.030.164223
TATCAAC60300.029.2784752
AACGCAG61700.028.4347766
GTACATG85150.024.8410451
TACATGG88150.023.3663162
ACGCAGA74600.023.0075657
ACATGGG91050.022.4135883
CGCAGAG76750.021.9294228
CATGGGG78150.020.7692094
GTATATA12000.018.617061
GTATACG2051.5814278E-518.549431
GCAGAGT89300.018.1553739
AGAGTAC73400.018.03978710-11
ATGGGGG48450.016.193125
GTATAGA9200.016.0165251
CAGAGTA82300.015.91563910-11
Produced by FastQC (version 0.11.5)